Cloned (Comment) | Organism |
---|---|
cloning and expression of the sso1354 gene in different hosts, Escherichia coli and Sulfolobus solfataricus itself | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Saccharolobus solfataricus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35154 | - |
x * 35154, enzyme recombinantly expressed in Escherichia coli, calculated from sequence | Saccharolobus solfataricus |
35500 | - |
x * 35500, enzyme recombinantly expressed in Escherichia coli, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97YG7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
pH 5.0, specific activity of the enzyme recombinantly expressed in Sulfolobus solfataricus is about 200fold higher compared to the specific activity of the enzyme recombinantly expressed in Escherichia coli | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl-cellulose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
cellulose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
curdlan + H2O | activity is 26% compared to activity with carboxymethyl-cellulose | Saccharolobus solfataricus | ? | - |
? | |
debranched arabinan | activity is 25% compared to activity with carboxymethyl-cellulose | Saccharolobus solfataricus | ? | - |
? | |
lichenan + H2O | activity is 120% compared to activity with carboxymethyl-cellulose | Saccharolobus solfataricus | ? | - |
? | |
additional information | no hydrolysis of p-nitrophenyl-beta-D-cellobioside and p-nitrophenyl-beta-D-cellotrioside, no exocellulase activity | Saccharolobus solfataricus | ? | - |
? | |
oat spelt xylan + H2O | activity is 25% compared to activity with carboxymethyl-cellulose | Saccharolobus solfataricus | ? | - |
? | |
pachyman + H2O | activity is 22% compared to activity with carboxymethyl-cellulose | Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35154, enzyme recombinantly expressed in Escherichia coli, calculated from sequence | Saccharolobus solfataricus |
? | x * 35500, enzyme recombinantly expressed in Escherichia coli, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
SSO1354 enzyme | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
enzyme recombinantly expressed in Sulfolobus solfataricus | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 180 min | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
recombinant enzyme from Sulfolobus solfataricus | Saccharolobus solfataricus |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is responsible for the main cellulase activity of Sulfolobus solfataricus | Saccharolobus solfataricus |