EC Number   |
Reference |
|---|
   3.4.22.15 | recombinant His6-tagged mature domain of CsCPL (CsCPL-m) solubilized from Escherichia coli inclusion bodies by dialysis through stepwise dilution of urea (6 to 1 M) in 20 mM Tris-HCl and 5 mM EDTA buffer (pH 8.0). Finally, the urea is removed by dialysis using phosphate-buffered saline, pH 7.4 |
754985 |
| 3.4.22.15 | the optimum cathepsin L renaturation buffer consists of 50 mM Tris, 4 mM GSH, 0.5 mM GSSG, 300 mM MgSO4, 0.05% (w/v) 3-(3-cholamidopropyl)-dimethylammonio-1-propane sulfonate, 0.04 mM propetide, at pH 7.75 and 16°C |
711345 |
| 3.4.22.15 | using 100 mM Tris-HCl, pH 8.0, 1 mM EDTA, 20% (v/v) glycerol, 250 mM L-arginine, 2 mM reduced glutathione, 1 mM oxidized glutathione |
712384 |
