Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli M15[pRep4] cell | Toxoplasma gondii |
Crystallization (Comment) | Organism |
---|---|
in complex with propeptide, sitting drop vapor diffusion method, using 40% (w/v) polyethylene glycol 8000, 0.1 M ammonium bromide, and 0.1 M sodium citrate, at pH 4.0 and 25°C | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
morpholinurea-leucyl-homophenyl-vinyl sulfone phenyl | moderate inhibitor | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | CPL is associated with a discrete vesicular structure in the apical region of extracellular parasites but is found in multiple puncta throughout the cytoplasm of intracellular replicating parasites | Toxoplasma gondii | 5737 | - |
vesicle | CPL is associated with a discrete vesicular structure in the apical region of extracellular parasites but is found in multiple puncta throughout the cytoplasm of intracellular replicating parasites | Toxoplasma gondii | 31982 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q6DMN0 | strain RH | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Toxoplasma gondii |
Renatured (Comment) | Organism |
---|---|
using 100 mM Tris-HCl, pH 8.0, 1 mM EDTA, 20% (v/v) glycerol, 250 mM L-arginine, 2 mM reduced glutathione, 1 mM oxidized glutathione | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Toxoplasma gondii | benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CPL | - |
Toxoplasma gondii |