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Literature summary for 3.4.22.15 extracted from

  • Larson, E.T.; Parussini, F.; Huynh, M.H.; Giebel, J.D.; Kelley, A.M.; Zhang, L.; Bogyo, M.; Merritt, E.A.; Carruthers, V.B.
    Toxoplasma gondii cathepsin L is the primary target of the invasion-inhibitory compound morpholinurea-leucyl-homophenyl-vinyl sulfone phenyl (2009), J. Biol. Chem., 284, 26839-26850.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15[pRep4] cell Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with propeptide, sitting drop vapor diffusion method, using 40% (w/v) polyethylene glycol 8000, 0.1 M ammonium bromide, and 0.1 M sodium citrate, at pH 4.0 and 25°C Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
morpholinurea-leucyl-homophenyl-vinyl sulfone phenyl moderate inhibitor Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm CPL is associated with a discrete vesicular structure in the apical region of extracellular parasites but is found in multiple puncta throughout the cytoplasm of intracellular replicating parasites Toxoplasma gondii 5737
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vesicle CPL is associated with a discrete vesicular structure in the apical region of extracellular parasites but is found in multiple puncta throughout the cytoplasm of intracellular replicating parasites Toxoplasma gondii 31982
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Organism

Organism UniProt Comment Textmining
Toxoplasma gondii Q6DMN0 strain RH
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Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Toxoplasma gondii

Renatured (Commentary)

Renatured (Comment) Organism
using 100 mM Tris-HCl, pH 8.0, 1 mM EDTA, 20% (v/v) glycerol, 250 mM L-arginine, 2 mM reduced glutathione, 1 mM oxidized glutathione Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O
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Toxoplasma gondii benzyloxycarbonyl-L-Phe-L-Arg + 7-amino-4-methylcoumarin
-
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Synonyms

Synonyms Comment Organism
CPL
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Toxoplasma gondii