EC Number   |
|---|
   3.5.1.93 | - |
| 3.5.1.93 | a modified chitin-binding domain-GLA fusion protein is affinity purified on chitin column by changing the salt concentration of binding and elution buffer |
| 3.5.1.93 | acetone precipitation and Sepharose CM column chromatography |
| 3.5.1.93 | ammonium sulfate precipitation, chitin granula column chromatography, and Co2+-iminodiacetic acid-Sepharose column chromatography |
| 3.5.1.93 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, TEAE-cellulose chromatography, Toyopearl HW65F column chromatography, CM Toyopearl 650 column chromatography, Sephadex G-200 gel filtration, Q-Sepharose column chromatography, hydroxyapatite column chromatography, SP-Trisacryl gel filtration, and Sephacryl S-300gel filtration |
| 3.5.1.93 | ammonium sulfate precipitation, Toyopearl HW65F column chromatography, and CM Toyopearl 650 column chromatography |
| 3.5.1.93 | by HiTrap chelating affinity chromatography |
| 3.5.1.93 | Cu2+-immobilized iminodiacetic acid column chromatography |
| 3.5.1.93 | Ni2+-HiTrap chelating column chromatography and Cu2+-immobilized iminodiacetic acid affinity chromatography |
| 3.5.1.93 | overexpressed mutant protein |
