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(5R,6R)-6-[(4-carboxybutanoyl)amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid + H2O
?
-
118% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
(6R,7R)-7-[(3-carboxypropanoyl)amino]-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + H2O
?
-
26.9% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
(6R,7R)-7-[(3-carboxypropanoyl)amino]-8-oxo-3-(propionyloxy)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + H2O
?
-
2.2% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
(6R,7R)-7-[(4-carboxybutanoyl)amino]-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + H2O
?
-
111% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
(6R,7R)-7-[(5-carboxypentanoyl)amino]-3-methyl-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + H2O
?
-
8.3% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
(6R,7R)-7-[(5-carboxypentanoyl)amino]-8-oxo-3-(propionyloxy)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid + H2O
?
-
6.9% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
2-amino-5-[(4-nitrophenyl)amino]-5-oxopentanoic acid + H2O
?
-
-
-
-
?
4-(glutarylamino)benzoic acid + H2O
glutarate + 4-aminobenzoate
-
-
-
-
?
5-oxo-5-(1-phenylethoxy)pentanoic acid + H2O
glutarate + ?
-
-
-
-
?
5-oxo-5-[(1-phenylethyl)amino]pentanoic acid + H2O
?
-
-
-
-
?
5-[(2-methoxy-1-phenylprop-2-en-1-yl)amino]-5-oxopentanoic acid + H2O
?
-
-
-
-
?
5-[(2-methoxy-1-phenylprop-2-en-1-yl)oxy]-5-oxopentanoic acid + H2O
?
-
-
-
-
?
5-[(4-nitrophenyl)amino]-5-oxopentanoic acid + H2O
?
-
-
-
-
?
5-[[(1S)-1-benzyl-2-methoxy-2-oxoethyl]amino]-5-oxopentanoic acid + H2O
?
-
-
-
-
?
7beta-(4-carboxylbutanamino)cephalosporanic acid + H2O
7-aminocephalosporanic acid + pentanoate
aculeacin A + H2O
cyclo-hexapeptide + palmitic acid
enzyme binding site mutants CAAC1 and CAAC2, no activity with the wild-type enzyme
-
-
?
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + adipate
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + adipate
adipyl-7-aminocephalosporanic acid + H2O
adipate + 7-aminocephalosporanate
-
-
-
-
?
adipyl-7-aminodeacetoxycephalosporanic acid + H2O
adipate + 7-aminodeacetoxycephalosporanic acid
-
-
-
-
?
adipyl-7-aminodesacetoxycephalosporanic acid + H2O
?
adipyl-7-aminodesacetoxycephalosporanic acid + H2O
adipate + 7-aminodesacetoxycephalosporanate
-
57.4% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
adipyl-7-aminodesacetoxycephalosporanic acid + H2O
deacetoxycephalosporanic acid + adipate
adipyl-aminopenicillanic acid + H2O
adipate + aminopenicillanic acid
wild-type enzyme shows 70.6% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
cephalosporin C + H2O
(7R)-7-aminocephalosporanate + 2-aminoadipate
cephalosporin C + H2O
7-aminocephalosporanate + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
cephalosporin C + H2O
7-aminocephalosporanic acid + ?
-
-
-
-
?
cephalosporin C + H2O
cephalosporamic acid + 2-amino-5-hydroxypentanoate
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
D-glutamyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + D-glutamate
-
activity is 0.85% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
D-glutamyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutamate
DGDPP + H2O
L-Asp + GDPP
-
20% activity compared to E-GDPPDLADQG
-
-
?
dimethyl glutarate + (2S)-2-amino-3-(4-hydroxyphenyl)propanamide
5-[[(1S)-2-amino-1-(4-hydroxybenzyl)-2-oxoethyl]amino]-5-oxopentanoic acid + ?
-
the enzyme catalyzes the synthesis of the amide bond when working at very high substrate concentrations, namely in heterogeneous substrate mixtures, whereas no enzymatic activity is detected using the enzyme in diluted organic solvent solutions. A negative charge on the acyl moiety is crucial for substrate recognition
-
-
?
dimethyl glutarate + ethyl (2S)-2-amino-3-(4-hydroxyphenyl)propanoate
5-[[(1S)-2-ethoxy-1-(4-hydroxybenzyl)-2-oxoethyl]amino]-5-oxopentanoic acid + ?
-
the enzyme catalyzes the synthesis of the amide bond when working at very high substrate concentrations, namely in heterogeneous substrate mixtures, whereas no enzymatic activity is detected using the enzyme in diluted organic solvent solutions. A negative charge on the acyl moiety is crucial for substrate recognition
-
-
?
EGDPP + H2O
L-Glu + GDPP
-
67% activity compared to E-GDPPDLADQG
-
-
?
EGDPPDLADQG + H2O
L-Glu + GDPPDLADQG
-
100% activity
-
-
?
gamma-Glu-Cys-Gly
L-glutamate + Cys-Gly
-
-
-
-
?
GEGDPP + H2O
Gly-L-Glu + GDPP
-
5% activity compared to E-GDPPDLADQG
-
-
?
glutaryl 7-aminodeacetoxycephalosporanic acid + H2O
7-aminodeacetoxycephalosporanic acid + glutarate
-
-
-
?
glutaryl deacetoxy 7-aminocephalosporanic acid + H2O
deacetoxycephalosporanic acid + glutarate
glutaryl deacetyl-7-aminocephalosporanic acid + H2O
deacetyl-7-aminocephalosporanic acid + glutarate
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid + H2O
glutarate + 3-chloro-7-aminodesacetoxycephalosporanate
-
81.9% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-7-amino cephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
glutaryl-7-aminocephalosporanic acid + H2O
glutarate + 7-aminocephalosporanate
-
9.9% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-7-aminodesacetoxycephalosporanic acid + H2O
glutarate + 7-aminodesacetoxycephalosporanate
-
71.5% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-alanyl-proline + H2O
glutarate + alanyl-proline
-
-
-
-
?
glutaryl-aminopenicillanic acid + H2O
glutarate + aminopenicillanic acid
wild-type enzyme shows 90.6% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-D-Phe-Gly + H2O
glutarate + D-Phe-Gly
-
about 25% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-deacetoxy-7-aminocephalosporanic acid + H2O
deacetoxycephalosporanic acid + glutarate
glutaryl-EGDPPDLADQG + H2O
glutarate + EGDPPDLADQG
-
-
-
-
?
glutaryl-gamma-Glu-Cys-Gly + H2O
glutarate + gamma-Glu-Cys-Gly
-
-
-
-
?
glutaryl-GDAADAADKG + H2O
glutarate + GDAADAADKG
-
-
-
-
?
glutaryl-GDPP + H2O
glutarate + GDPP
-
substrate with highest catalytic efficiency
-
-
?
glutaryl-GDPPDLADQG + H2O
glutarate + GDPPDLADQG
-
-
-
-
?
glutaryl-GGGGAA + H2O
glutarate + GGGGAA
-
-
-
-
?
glutaryl-GGGGGK + H2O
glutarate + GGGGGK
-
-
-
-
?
glutaryl-glycine + H2O
glutarate + glycine
glutaryl-glycyl-L-proline + H2O
glutarate + glycyl-L-proline
-
-
-
-
?
glutaryl-glycyl-proline + H2O
glutarate + glycylproline
-
-
-
-
?
glutaryl-L-Ala + H2O
glutarate + L-Ala
-
about 25% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-L-Phe + H2O
glutarate + L-Phe
-
about 10% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-L-Phe-Gly + H2O
glutarate + L-Phe-Gly
-
about 50% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-tryptophan + H2O
glutarate + tryptophan
-
-
-
-
?
glutarylaniline + H2O
glutarate + aniline
-
-
-
-
?
L-gamma-glutamyl p-nitroanilide + H2O
L-glutamate + p-nitroaniline
N-acetyl cephalosporin C + H2O
acetate + cephalosporin C
N-glutaryl-D-alanine methyl ester + H2O
glutarate + D-alanine methyl ester
-
2.2% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-D-phenylalanine + H2O
glutarate + D-Phe
-
2% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-D-phenylalanine methyl ester + H2O
glutarate + D-phenylalanine methyl ester
-
4.5% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-D-phenylglycine + H2O
glutarate + D-phenylglycine
-
1.1% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-D-phenylglycine methyl ester + H2O
glutarate + D-phenylglycine methyl ester
-
0.23% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-L-Ala + H2O
glutarate + L-Ala
-
2.7% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-L-alanine methyl ester + H2O
glutarate + L-alanine methyl ester
-
25% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-L-phenylalanine + H2O
glutarate + L-Phe
-
2.5% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-L-phenylalanine methyl ester + H2O
glutarate + L-phenylalanine methyl ester
-
15.7% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-L-phenylglycine + H2O
glutarate + L-phenylglycine
-
7.5% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutaryl-L-phenylglycine methyl ester + H2O
glutarate + L-phenylglycine methyl ester
-
59.3% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-glutarylglycine methyl ester + H2O
glutarate + glycine methyl ester
-
9.7% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
penicillin G + H2O
(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
low activity
-
-
?
pentanoyl-7-aminocephalosporanic acid + H2O
pentanoate + 7-aminocephalosporanate
-
about 2.4% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
succinyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + succinate
succinyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + succinate
succinyl-7-aminocephalosporanic acid + H2O
succinate + 7-aminocephalosporanate
-
25.8% activity with 5 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
additional information
?
-
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
-
-
-
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
-
-
-
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
-
-
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
-
-
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
-
-
-
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
-
-
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
-
-
-
?
(7R)-7-(4-carboxybutanamido)cephalosporanate + H2O
(7R)-7-aminocephalosporanate + glutarate
GL-7-ACA
-
-
?
7beta-(4-carboxylbutanamino)cephalosporanic acid + H2O
7-aminocephalosporanic acid + pentanoate
-
-
-
-
?
7beta-(4-carboxylbutanamino)cephalosporanic acid + H2O
7-aminocephalosporanic acid + pentanoate
-
-
-
-
?
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + adipate
-
activity is 19% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + adipate
-
activity is 19% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + adipate
-
activity is 68% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + adipate
-
activity is 68% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + adipate
-
activity is 0.56% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + adipate
-
-
-
-
?
adipyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + adipate
-
-
-
?
adipyl-7-aminodesacetoxycephalosporanic acid + H2O
?
-
-
-
-
?
adipyl-7-aminodesacetoxycephalosporanic acid + H2O
?
-
-
-
-
?
adipyl-7-aminodesacetoxycephalosporanic acid + H2O
deacetoxycephalosporanic acid + adipate
-
-
-
?
adipyl-7-aminodesacetoxycephalosporanic acid + H2O
deacetoxycephalosporanic acid + adipate
-
-
-
?
cephalosporin C + H2O
(7R)-7-aminocephalosporanate + 2-aminoadipate
-
-
-
-
?
cephalosporin C + H2O
(7R)-7-aminocephalosporanate + 2-aminoadipate
-
-
-
?
cephalosporin C + H2O
(7R)-7-aminocephalosporanate + 2-aminoadipate
-
the wild-type enzyme shows weak activity with cephalosporin C, but some enzyme mutants show increased activity
-
-
?
cephalosporin C + H2O
(7R)-7-aminocephalosporanate + 2-aminoadipate
-
the wild-type enzyme shows weak activity with cephalosporin C, while the activity of double mutant H57betaS/H70betaS with this substrate is enhanced
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
activity determined using a biological and colorimetic method. For the spectrophotometric assay p-dimethylaminobenzaldehyde is added to produce a yellow condensation product which can be measured at a wavelength of 414 nm
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
low activity
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
CPC, low activity
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
7-aminocephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
?
cephalosporin C + H2O
cephalosporamic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporamic acid + 2-amino-5-hydroxypentanoate
the recombinant enzyme exhibits 2.3times more cephalosporin C specific deacylation activity compared to glutaryl-7-aminocephalosporanic acid
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
weak activity
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
weak activity
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
activity is 4% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
activity is 4% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
weak activity
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
low activity
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
wild-type enzyme shows 2.3% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
double mutant H296S/H309S exhibits 22fold enhanced specificity and reactivity for cephalosporin C over the natural substrate glutaryl-7-aminocephalosporanic acid
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
low activity
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
low activity
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
low activity
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
cephalosporin C + H2O
cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
-
-
-
?
D-glutamyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutamate
-
activity is 0.55% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
D-glutamyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutamate
-
activity is 0.55% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl deacetoxy 7-aminocephalosporanic acid + H2O
deacetoxycephalosporanic acid + glutarate
-
-
-
-
?
glutaryl deacetoxy 7-aminocephalosporanic acid + H2O
deacetoxycephalosporanic acid + glutarate
-
-
-
-
?
glutaryl deacetyl-7-aminocephalosporanic acid + H2O
deacetyl-7-aminocephalosporanic acid + glutarate
-
-
-
-
?
glutaryl deacetyl-7-aminocephalosporanic acid + H2O
deacetyl-7-aminocephalosporanic acid + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
rate of synthesis is slower than rate of hydrolysis
-
-
r
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
rate of synthesis is slower than rate of hydrolysis
-
-
r
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
rate of synthesis is fold slower than rate of synthesis
-
-
r
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
rate of synthesis is fold slower than rate of synthesis
-
-
r
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
660711, 660734, 660735, 660933, 661225, 671010, 686238, 686445, 686677, 687871, 719942, 720077 -
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
100% activity
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
rate of synthesis is slower than rate of hydrolysis
-
-
r
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
-
activity assay
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
-
double mutant H296S/H309S exhibits 22fold enhanced specificity and reactivity for cephalosporin C over the natural substrate glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
GL-7-ACA
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
-
-
-
-
?
glutaryl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + glutarate
-
-
-
?
glutaryl-deacetoxy-7-aminocephalosporanic acid + H2O
deacetoxycephalosporanic acid + glutarate
-
activity is 2.94fold higher than with glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-deacetoxy-7-aminocephalosporanic acid + H2O
deacetoxycephalosporanic acid + glutarate
-
activity is 2.94fold higher than with glutaryl-7-aminocephalosporanic acid
-
-
?
glutaryl-glycine + H2O
glutarate + glycine
-
-
-
-
?
glutaryl-glycine + H2O
glutarate + glycine
-
less than 5% activity with 50 mM substrate compared to glutaryl-7-aminocephalosporanic acid
-
-
?
L-gamma-glutamyl p-nitroanilide + H2O
L-glutamate + p-nitroaniline
-
-
-
-
?
L-gamma-glutamyl p-nitroanilide + H2O
L-glutamate + p-nitroaniline
-
-
-
-
?
L-gamma-glutamyl p-nitroanilide + H2O
L-glutamate + p-nitroaniline
-
-
-
-
?
L-gamma-glutamyl p-nitroanilide + H2O
L-glutamate + p-nitroaniline
-
-
-
-
?
N-acetyl cephalosporin C + H2O
acetate + cephalosporin C
-
activity is 1% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
N-acetyl cephalosporin C + H2O
acetate + cephalosporin C
-
activity is 1% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
succinyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + succinate
-
activity is 32% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
succinyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + succinate
-
activity is 32% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
succinyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanate + succinate
-
-
-
-
?
succinyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + succinate
-
activity is 6.1% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
succinyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + succinate
-
activity is 6.1% of the activity with glutaryl-7-aminocephalosporanic acid
-
-
?
succinyl-7-aminocephalosporanic acid + H2O
7-aminocephalosporanic acid + succinate
-
activity is 9.9fold higher than with glutaryl-7-aminocephalosporanic acid
-
-
?
additional information
?
-
-
enzyme also exhibits L-gamma-glutamyltranspeptidase activity
-
-
?
additional information
?
-
-
no activity with cephalosporin C and succinyl-4-aminocephalosporanic acid
-
-
?
additional information
?
-
-
enzyme also exhibits L-gamma-glutamyltranspeptidase activity
-
-
?
additional information
?
-
-
no activity with cephalosporin C and succinyl-4-aminocephalosporanic acid
-
-
?
additional information
?
-
-
purified enzyme exhibits also gamma-glutamyltranspeptidase activity with L-gamma-glutamyl-p-nitroanilide
-
-
?
additional information
?
-
-
the enzyme has transacylase activity 10times that of its hydrolytic activity
-
-
?
additional information
?
-
-
less than 1% activity with TLGEGDPP compared to EGDPPDLADQG, no activity with adipyl-7-aminocephalosporanic acid
-
-
?
additional information
?
-
-
the enzyme also removes N-terminal Glu or Asp from peptides. Cephalosporin acylase is an acylpeptide hydrolase rather than a previously expected acylamino acid acylase. It only exhibits exopeptidase activity for the hydrolysis of an externally added peptide, supporting the intra-molecular interaction
-
-
?
additional information
?
-
-
the precursor cephalosporin acylase (without signal peptide) is activated by two peptide bond cleavages, which excise an internal spacer. The first cleavage occurs between Gly169 and Ser170 and the second cleavage occurs between Glu159 and Gly160
-
-
?
additional information
?
-
-
the recombinant enzyme shows no activity towards cephalosporin C, butanoyl-7-aminodesacetoxycephalosporanic acid and pentanoyl-7-aminodesacetoxycephalosporanic acid
-
-
?
additional information
?
-
-
the amino acid moiety of cephalosporin C that are potentially involved in the enzyme substrate selectivity are Met165alpha, His23beta, Arg24beta, Val25beta, Tyr32beta, Phe55beta, Phe58beta, Phe72beta, Leu154beta, Leu175beta, Ile176beta and His178beta, substrate docking in the active site, overview
-
-
?
additional information
?
-
substrate chephalosporin has a similar negative charge in pH 8.5 solution
-
-
-
additional information
?
-
-
the enzyme has transacylase activity 10times that of its hydrolytic activity
-
-
?
additional information
?
-
the amount of product 7-aminocephalosporanic acid formed is measured by glutaryl p-nitroanilide (GpNA), 4-dimethyl aminobenzaldehyde (p-DAB), and HPLC assays
-
-
-
additional information
?
-
one-pot conversion of cephalosporin C to 7-aminocephalosporanic acid (7-ACA) using an optimized two-enzyme process, involving D-amino acid oxidase (DAAO, EC 1.4.3.3) from Rhodotorula gracilis and glutaryl-7-aminocephalosporanic acid acylase (GA, EC 3.5.1.93) from Pseudomonas sp. N176. The flavoenzyme DAAO oxidizes the D-2-aminoadipyl moiety of CephC to give 2-oxoadipyl-7-ACA, which is converted to glutaryl-7-ACA (Gl-7-ACA) nonenzymatically. Concerning the simultaneous action of DAAO and GA for 7-ACA production in a single reactor, the main problems are the presence of H2O2 during the reaction process (produced by the DAAO reaction and required to push decarboxylation of oxo-7-ACA into Gl-7-ACA), which can inactivate the enzymes being employed, especially DAAO, and the substrate/product inhibition effects observed with GA
-
-
-
additional information
?
-
the D-2-aminoadipyl side chain of CPC is much larger than the glutaryl side chain of GL-7-ACA, and the activity of N176 acylase toward CPC is only 4% compared with the activity toward GL-7-ACA
-
-
-
additional information
?
-
a cephalosporin acylase enzyme mutant with binding pocket altered for the binding of long acyl chain shows lower enzymatic activity with cephalosporin but higher activity with aculeacin A, in comparison with the wild-type enzyme
-
-
-
additional information
?
-
-
purified enzyme exhibits also gamma-glutamyltranspeptidase activity with L-gamma-glutamyl-p-nitroanilide
-
-
?
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0.041 - 21.9
(7R)-7-(4-carboxybutanamido)cephalosporanate
4.5
4-(glutarylamino)benzoic acid
-
at 37°C, pH not specified in the publication
0.16 - 12
adipyl-7-aminocephalosporanic acid
0.14 - 13.8
adipyl-7-aminodeacetoxycephalosporanic acid
0.14 - 7.9
adipyl-7-aminodesacetoxycephalosporanic acid
1.5 - 121
cephalosporin C
3.3
gamma-Glu-Cys-Gly
-
at 37°C, pH not specified in the publication
1.6
glutaryl deacetoxy 7-aminocephalosporanic acid
-
-
1 - 8.7
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid
0.7 - 37.2
glutaryl-7-amino cephalosporanic acid
0.016 - 17.9
glutaryl-7-aminocephalosporanic acid
1.3 - 14.1
glutaryl-7-aminodesacetoxycephalosporanic acid
0.73
glutaryl-alanyl-proline
-
at 37°C, pH not specified in the publication
2.7
glutaryl-EGDPPDLADQG
-
at 37°C, pH not specified in the publication
6.4
glutaryl-gamma-Glu-Cys-Gly
-
at 37°C, pH not specified in the publication
0.4
glutaryl-GDAADAADKG
-
at 37°C, pH not specified in the publication
0.35
glutaryl-GDPP
-
at 37°C, pH not specified in the publication
0.67
glutaryl-GDPPDLADQG
-
at 37°C, pH not specified in the publication
1.68
glutaryl-GGGGAA
-
at 37°C, pH not specified in the publication
0.43
glutaryl-GGGGGK
-
at 37°C, pH not specified in the publication
3.1
glutaryl-glycine
-
at 37°C, pH not specified in the publication
0.85
glutaryl-glycyl-proline
-
at 37°C, pH not specified in the publication
6.1
glutaryl-tryptophan
-
at 37°C, pH not specified in the publication
40
glutarylaniline
-
at 37°C, pH not specified in the publication
5.92
L-gamma-glutamyl p-nitroanilide
-
-
3.8
L-gamma-glutamyl-p-nitroanilide
-
-
16.1 - 17.4
pentanoyl-7-aminocephalosporanic acid
2.8 - 5.4
succinyl-7-aminocephalosporanic acid
additional information
7-aminocephalosporanic acid
0.041
(7R)-7-(4-carboxybutanamido)cephalosporanate
pH 8.5, 37°C, free mutant enzyme
0.043
(7R)-7-(4-carboxybutanamido)cephalosporanate
pH 8.5, 37°C, free wild-type enzyme
0.0438
(7R)-7-(4-carboxybutanamido)cephalosporanate
pH 8.5, 37°C, immobilized mutant enzyme
0.0484
(7R)-7-(4-carboxybutanamido)cephalosporanate
pH 8.5, 37°C, immobilized wild-type enzyme
0.4
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/F58betaD, pH 8.0, 37°C
0.41
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/M165alphaS/I176betaS, pH 8.0, 37°C
0.6
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L176betaK, pH 8.0, 37°C
1.5
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant wild-type enzyme, pH 8.0, 25°C
1.5
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant wild-type enzyme, pH 8.0, 25°C
1.8
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L176betaT, pH 8.0, 37°C
2
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/F72betaR, pH 8.0, 37°C
2
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L154betaY, pH 8.0, 37°C
3.2
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L176betaQ, pH 8.0, 37°C
3.6
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/V25betaR, pH 8.0, 37°C
3.8
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L175betaT, pH 8.0, 37°C
6.3
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/F58betaI, pH 8.0, 37°C
6.6
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/L154betaY/M165alphaS, pH 8.0, 37°C
6.9
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS, pH 8.0, 25°C
9.2
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/R24betaP, pH 8.0, 37°C
21.9
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant H57betaS/H70betaS/M165alphaS, pH 8.0, 37°C
21.9
(7R)-7-(4-carboxybutanamido)cephalosporanate
-
recombinant mutant M165alphaS/H57betaS/H70betaS, pH 8.0, 25°C
0.16
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375G
0.17
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375C
0.51
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375A
0.65
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375M
0.7
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375L
0.7
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Y
0.83
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375N
0.9
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375H
0.9
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375T
0.99
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375S
1
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, wild-type enzyme
1.37
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375V
1.5
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375W
2.07
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375I
2.1
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Q
2.16
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375K
4.3
adipyl-7-aminocephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
4.4
adipyl-7-aminocephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
7.5
adipyl-7-aminocephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
10
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375R
11
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375D
12
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375E
0.14
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266H
0.33
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266M
0.59
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266W
0.6
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266F
0.62
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266Q
0.65
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266Y
0.8
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266C
0.99
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266S
1
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266L
1.2
adipyl-7-aminodeacetoxycephalosporanic acid
-
wild-type enzyme
1.4
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266G
2.2
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266A
2.2
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266D
4.4
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266P
5.7
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266T
13.8
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266E
0.14
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266H
0.14
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266H
0.17
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266Q/F375L
0.17
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266M
0.19
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266H
0.28
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266Q/F375L
0.33
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266M
0.42
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266S
0.47
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H
0.52
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme F229L
0.62
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266Q
0.65
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme F375M
0.7
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S
0.7
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme F375L
0.7
adipyl-7-aminodesacetoxycephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
0.8
adipyl-7-aminodesacetoxycephalosporanic acid
-
wild type enzyme
0.82
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme F375L
0.96
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, wild-type enzyme
3.8
adipyl-7-aminodesacetoxycephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
7.9
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
1.5
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/F58betaD, pH 8.0, 37°C
1.5
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L176betaC, pH 8.0, 37°C
1.7
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H296S/H417M
1.8
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/M165alphaS/I176betaS, pH 8.0, 37°C
2
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/R24betaP, pH 8.0, 37°C
2.3
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/V25betaR, pH 8.0, 37°C
2.7
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme Y271V
3.1
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L154betaY/M165alphaS, pH 8.0, 37°C
3.2
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme A215E
3.8
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L175betaT, pH 8.0, 37°C
4.1
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H296F
4.5
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L154betaY, pH 8.0, 37°C
4.5
cephalosporin C
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/L154betaY
4.8
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H296T
5.2
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H296N
5.4
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L176betaT, pH 8.0, 37°C
6.3
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H309Y
6.5
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme F270M
6.7
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H296S
6.9
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme A215Y
7.2
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme D416Y
7.5
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/L176betaK, pH 8.0, 37°C
7.7
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme A215F
8
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme Y271F
8.1
cephalosporin C
recombinant enzyme mutant M6, pH and temperature not specified in the publication
8.3
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme A215V
9.3
cephalosporin C
recombinant mutant L154betaF/L180betaF, pH 8.0, 37°C
9.5
cephalosporin C
-
recombinant wild-type enzyme, pH 8.0, 25°C
9.5
cephalosporin C
pH 8.0, 25°C, recombinant wild-type enzyme
9.5
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme A215L
9.5
cephalosporin C
-
wild type enzyme, at pH 8.0 and 25°C
9.5
cephalosporin C
-
recombinant mutant wild-type enzyme, pH 8.0, 25°C
9.73
cephalosporin C
recombinant mutant L154betaF, pH 8.0, 37°C
10
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme A215Y/H296S
10.1
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme Y215Y/F270S
10.14
cephalosporin C
recombinant mutant L180betaF, pH 8.0, 37°C
11
cephalosporin C
-
pH 8.0, 37°C, wild-type enzyme
11
cephalosporin C
recombinant wild-type enzyme, pH and temperature not specified in the publication
12.2
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H296S/H309S
12.2
cephalosporin C
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
12.2
cephalosporin C
-
recombinant mutant H57betaS/H70betaS, pH 8.0, 25°C
12.2
cephalosporin C
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS
12.3
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H417Y
12.38
cephalosporin C
recombinant mutant M31betaF/H57betaS/V68betaA/H70betaS, pH 8.0, 37°C
12.65
cephalosporin C
recombinant mutant M31betaF/H57betaS/H70betaS, pH 8.0, 37°C
13
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme D416Y/H417Y
16.4
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/F72betaR, pH 8.0, 37°C
16.4
cephalosporin C
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/F72betaR
17.2
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme A215Y/H309S
17.8
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme A215Y/H296S/H309S
18.9
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme E89A/A215Y
19.2
cephalosporin C
recombinant free enzyme, pH 8.5, 37°C
21.3
cephalosporin C
-
37°C, mutant enzyme Y269F
21.6
cephalosporin C
-
37°C, wild-type enzyme
22
cephalosporin C
-
at 37°C, pH not specified in the publication
22.1
cephalosporin C
-
37°C, mutant enzyme Y269Y
24.4
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme H309S
24.5
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme S22P/T394P/D416Y/H417Y
27.7
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/M165alphaS, pH 8.0, 37°C
27.7
cephalosporin C
-
recombinant mutant M165alphaS/H57betaS/H70betaS, pH 8.0, 25°C
27.8
cephalosporin C
-
37°C, mutant enzyme Y270F
32
cephalosporin C
-
pH 8.0, 37°C, mutant enzyme I44V/E49stop/D416Y/H417Y
33.7
cephalosporin C
-
recombinant mutant H57betaS/H70betaS/F58betaI, pH 8.0, 37°C
37
cephalosporin C
mutant enzyme L677F, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
37
cephalosporin C
pH 8.5, 37°C, recombinant mutant L677F
43
cephalosporin C
wild type enzyme, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
43
cephalosporin C
pH 8.5, 37°C, recombinant wild-type enzyme
51
cephalosporin C
-
deletion mutant D4(212-ADLA-215), pH 8.5, 37°C
51.6
cephalosporin C
-
wild-type enzyme, pH 8.5, 37°C
74.8
cephalosporin C
-
deletion mutant D2(227-AM-228), pH 8.5, 37°C
86
cephalosporin C
mutant enzyme P295A, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
86
cephalosporin C
pH 8.5, 37°C, recombinant mutant P295A
95.1
cephalosporin C
recombinant immobilized enzyme, pH 8.5, 37°C
105
cephalosporin C
mutant enzyme A675G, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
105
cephalosporin C
pH 8.5, 37°C, recombinant mutant A675G
121
cephalosporin C
mutant enzyme H309V, in 100 mM Tris-HCl buffer, pH 8.0, temperature not specified in the publication
121
cephalosporin C
pH 8.5, 37°C, recombinant mutant H309V
1
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
2.2
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
8.7
glutaryl-3-chloro-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
0.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296S
0.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215F
0.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215Y/H309S
1
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215E
1
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H309Y
1.1
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme E89A/A215Y
1.2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215V
1.2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y271F
1.6
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, wild-type enzyme
1.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215L
1.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215Y
1.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme D416Y
1.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296F
1.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y27V
2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296T
2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y215Y/F270S
2.5
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215Y/H296S/H309S
2.7
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme F270M
2.8
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296N
4
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme A215Y/H296S
4.3
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H417Y
4.6
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H309S
5.5
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296S/H417M
6.9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme H296S/H309S
9
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme D416Y/H417Y
25
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme I44V/E49stop/D416Y/H417Y
37.2
glutaryl-7-amino cephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme S22P/T394P/D416Y/H417Y
0.016
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266H
0.031
glutaryl-7-aminocephalosporanic acid
-
wild-type enzyme
0.031
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, wild-type enzyme
0.033
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, wild-type enzyme
0.038
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375C
0.04
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266H
0.042
glutaryl-7-aminocephalosporanic acid
-
wild type enzyme
0.043
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S
0.044
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266H
0.048
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme F229L
0.066
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375G
0.07
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375A
0.07
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375S
0.075
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266S
0.08
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Y
0.08
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme Y178H
0.08
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme Y178H/N266H
0.08
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme Y178H/N266Q/F375L
0.09
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375T
0.094
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266S
0.1
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266F
0.1
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375I
0.1
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375V
0.11
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266Y
0.11
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375H
0.12
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266Q
0.12
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266Q
0.15
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y126F
0.16
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266C
0.17
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266M
0.17
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266M
0.17
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375M
0.17
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme F375M
0.17
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266Q/F375L
0.18
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266W
0.18
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375N
0.19
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, wild-type enzyme
0.2
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375E
0.21
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, wild-type enzyme
0.22
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375D
0.26
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266A
0.26
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375L
0.26
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme F375L
0.27
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme F375L
0.29
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266E
0.3
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme Y178H/N266M
0.33
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4A
0.33
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4Y
0.36
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4T
0.37
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4F
0.37
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4L
0.4
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4H
0.43
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266L
0.46
glutaryl-7-aminocephalosporanic acid
-
-
0.5
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, wild-type enzyme
0.5
glutaryl-7-aminocephalosporanic acid
-
at 37°C, pH not specified in the publication
0.54
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266G
0.6
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375R
0.7
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375K
0.7
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Q
0.8
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375W
0.82
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme H23Q
1.18
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme E455Q
1.2
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme W173A
1.24
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme E455D
1.3
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266P
1.5
glutaryl-7-aminocephalosporanic acid
pH 8.0, 25°C, recombinant wild-type enzyme
1.5
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266T
1.5
glutaryl-7-aminocephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
1.69
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y126A
1.76
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme H23D
2
glutaryl-7-aminocephalosporanic acid
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/F72betaR
2
glutaryl-7-aminocephalosporanic acid
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS/L154betaY
2.1
glutaryl-7-aminocephalosporanic acid
-
-
2.5
glutaryl-7-aminocephalosporanic acid
-
37°C, mutant enzyme M164A
2.5
glutaryl-7-aminocephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
2.57
glutaryl-7-aminocephalosporanic acid
-
37°C, mutant enzyme M164G
2.6
glutaryl-7-aminocephalosporanic acid
-
-
3
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
3.1
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunit mutant enzyme E455L
3.2
glutaryl-7-aminocephalosporanic acid
-
-
3.3
glutaryl-7-aminocephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
3.68
glutaryl-7-aminocephalosporanic acid
-
37°C, mutant enzyme M164N
5.4
glutaryl-7-aminocephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
5.85
glutaryl-7-aminocephalosporanic acid
-
37°C, wild-type enzyme
6.1
glutaryl-7-aminocephalosporanic acid
-
-
6.9
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
6.9
glutaryl-7-aminocephalosporanic acid
pH 8.0, 25°C, recombinant mutant H57betaS/H70betaS
9.32
glutaryl-7-aminocephalosporanic acid
-
37°C, mutant enzyme M164Q
9.9
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, soluble enzyme
10.41
glutaryl-7-aminocephalosporanic acid
-
-
11.7
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme K198A
12.2
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme R121A
12.3
glutaryl-7-aminocephalosporanic acid
wild-type enzyme
12.7
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme D286A
13.4
glutaryl-7-aminocephalosporanic acid
mutant enzyme with mutation Y151F in alpha-subunit and mutation Q50N in beta-subunit
14.4
glutaryl-7-aminocephalosporanic acid
alpha-subunit mutant enzyme Y151F
15.4
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, enzyme immobilized on silica gel modified with 3-aminopropyltriethoxysilane
17.8
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme Q50N/K198A
17.9
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme Q50N
1.3
glutaryl-7-aminodesacetoxycephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
1.5
glutaryl-7-aminodesacetoxycephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
14.1
glutaryl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
16.1
pentanoyl-7-aminocephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
17.4
pentanoyl-7-aminocephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
2.8
succinyl-7-aminocephalosporanic acid
-
recombinant enzyme, at pH 8.0 and 25°C
2.8
succinyl-7-aminocephalosporanic acid
-
wild type enzyme, at pH 8.0 and 25°C
3.2
succinyl-7-aminocephalosporanic acid
-
at 37°C, pH not specified in the publication
5.4
succinyl-7-aminocephalosporanic acid
-
mutant enzyme H296S/H309S, at pH 8.0 and 25°C
additional information
7-aminocephalosporanic acid
-
estimated value: 9.43 mg/ml, pH 7.6, 34°C, Vmax: 7.65 U/ml
additional information
additional information
-
the apparent Km value of CPC acylase increases and Vmax is almost unaffected in the presence of 7-aminocephalosporanic acid
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes with cephalosporin C, overview
-
additional information
additional information
Michaelis-Menten kinetics of free and immobilized wild-type and mutant enzymes, overview
-
additional information
additional information
Michaelis constant (Km) and maximum reaction velocity (Vmax) for the free and immobilized enzyme are calculated according to Lineweaver-Burk plots
-
additional information
additional information
substrate binding to the protein surface follows a Langmuir model resulting in binding constants K = 7.4 and 9.2 mM for wild-type and mutant M6 enzymes, respectively, which are similar to the experimentally determined Michaelis constants, thermodynamics, overview
-
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0.16
4-(glutarylamino)benzoic acid
-
at 37°C, pH not specified in the publication
0.0485 - 0.508
7-aminocephalosporanic acid
0.015 - 1.16
adipyl-7-aminocephalosporanic acid
0.002 - 1.9
adipyl-7-aminodeacetoxycephalosporanic acid
0.34 - 1.9
adipyl-7-aminodesacetoxycephalosporanic acid
0.015 - 10.4
cephalosporin C
0.33
gamma-Glu-Cys-Gly
-
at 37°C, pH not specified in the publication
0.008 - 49.8
glutaryl-7-aminocephalosporanic acid
6.2
glutaryl-alanyl-proline
-
at 37°C, pH not specified in the publication
12.8
glutaryl-EGDPPDLADQG
-
at 37°C, pH not specified in the publication
3.2
glutaryl-gamma-Glu-Cys-Gly
-
at 37°C, pH not specified in the publication
4.8
glutaryl-GDAADAADKG
-
at 37°C, pH not specified in the publication
13.8
glutaryl-GDPP
-
at 37°C, pH not specified in the publication
13.2
glutaryl-GDPPDLADQG
-
at 37°C, pH not specified in the publication
1.36
glutaryl-GGGGAA
-
at 37°C, pH not specified in the publication
1.73
glutaryl-GGGGGK
-
at 37°C, pH not specified in the publication
1.65
glutaryl-glycine
-
at 37°C, pH not specified in the publication
5.4
glutaryl-glycyl-proline
-
at 37°C, pH not specified in the publication
3.1
glutaryl-tryptophan
-
at 37°C, pH not specified in the publication
0.35
glutarylaniline
-
at 37°C, pH not specified in the publication
1.28
succinyl-7-aminocephalosporanic acid
-
at 37°C, pH not specified in the publication
additional information
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y126F
0.0485
7-aminocephalosporanic acid
-
pH and temperature not specified in the publication
0.508
7-aminocephalosporanic acid
-
D445G mutant, pH and temperature not specified in the publication
0.015
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375D
0.018
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375R
0.026
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375W
0.036
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375K
0.073
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375G
0.08
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375E
0.09
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375V
0.097
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375I
0.11
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375S
0.2
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375A
0.24
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375T
0.43
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Y
0.49
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, wild-type enzyme
0.55
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375C
0.65
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375M
0.67
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375L
0.68
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Q
0.9
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375N
1.16
adipyl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375H
0.002
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266D
0.023
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266E
0.061
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266P
0.15
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266T
0.18
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266A
0.23
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266G
0.24
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266S
0.25
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266L
0.31
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266C
0.33
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266W
0.33
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266Y
0.37
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266F
0.41
adipyl-7-aminodeacetoxycephalosporanic acid
-
wild-type enzyme
0.46
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266Q
0.47
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266H
1.9
adipyl-7-aminodeacetoxycephalosporanic acid
-
mutant enzyme N266M
0.34
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266S
0.38
adipyl-7-aminodesacetoxycephalosporanic acid
-
wild type enzyme
0.46
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, wild-type enzyme
0.46
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266Q
0.47
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266H
0.47
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme F229L
0.48
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266Q/F375L
0.55
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266H
0.61
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S
0.65
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme F375M
0.66
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H
0.66
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266Q/F375L
0.67
adipyl-7-aminodesacetoxycephalosporanic acid
pH 7.5, 37°C, mutant enzyme F375L
0.67
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme F375L
0.95
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266H
1.2
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme Y178H/N266M
1.9
adipyl-7-aminodesacetoxycephalosporanic acid
-
mutant enzyme N266M
0.015
cephalosporin C
-
at 37°C, pH not specified in the publication
0.022
cephalosporin C
-
wild-type enzyme and deletion mutant D4(212-ADLA-215), pH 8.5, 37°C
0.023
cephalosporin C
-
deletion mutant D2(227-AM-228), pH 8.5, 37°C
2.91
cephalosporin C
-
37°C, mutant enzyme Y270F
5.93
cephalosporin C
-
37°C, wild-type enzyme
9.4
cephalosporin C
-
37°C, mutant enzyme Y269Y
10.4
cephalosporin C
-
37°C, mutant enzyme Y269F
0.008
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375E
0.01
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375D
0.025
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266E
0.031 - 0.51
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, wild-type enzyme
0.082
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266L
0.13
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266P
0.21
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme Q50N/K198A
0.21
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375R
0.22
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme Q50N
0.23
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme W173A
0.24
glutaryl-7-aminocephalosporanic acid
alpha-subunit mutant enzyme Y151F
0.38
glutaryl-7-aminocephalosporanic acid
mutant enzyme with mutation Y151F in the alpha-subunit and Q50N in the beta-subunit
0.41
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme D286A
0.45
glutaryl-7-aminocephalosporanic acid
wild-type enzyme
0.45
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme R121A
0.47
glutaryl-7-aminocephalosporanic acid
beta-subunit mutant enzyme K198betaA
0.47
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375W
0.49
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266T
0.61
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266Y
0.67
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375I
0.75
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266Q/F375L
0.8
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Q
0.89
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme Y178H/N266M
0.9
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266F
1.02
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375V
1.1
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375L
1.1
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme F375L
1.1
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme F375L
1.3
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266W
1.334
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375H
1.4
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266A
1.6
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266S
1.8
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266M
1.8
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266M
1.8
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375T
1.96
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375N
2
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266C
2
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266G
2.02
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375M
2.02
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme F375M
2.2
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375K
2.2
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266S
2.2
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme Y178H/N266H
2.308
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375C
2.35
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375A
2.4
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266Q
2.4
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266Q
2.46
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375G
2.5
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme Y178H
2.5
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme Y178H/N266Q/F375L
3.1
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266H
3.1
glutaryl-7-aminocephalosporanic acid
-
mutant enzyme N266H
3.1
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme F229L
3.1
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375S
3.3
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme N266H
3.3
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4T
3.4
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme E455L
3.5
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, mutant enzyme M271V/Q291K/T374S
3.6
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4A
3.64
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, mutant enzyme F375Y
3.8
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4L
4
glutaryl-7-aminocephalosporanic acid
-
wild-type enzyme
4
glutaryl-7-aminocephalosporanic acid
-
pH 7.5, 37°C, wild-type enzyme
4.1
glutaryl-7-aminocephalosporanic acid
-
wild type enzyme
4.3
glutaryl-7-aminocephalosporanic acid
pH 7.5, 37°C, wild-type enzyme
5
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4F
5.5
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4H
5.9
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, beta-subunit mutant W4Y
6.05
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y126F
6.1
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme H23D
6.5
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunit mutant enzyme H23Q
6.6
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme E455Q
7.1
glutaryl-7-aminocephalosporanic acid
-
at 37°C, pH not specified in the publication
7.16
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, mutant enzyme Y126A
7.54
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, wild-type enzyme
7.8
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 37°C, wild-type enzyme
8.29
glutaryl-7-aminocephalosporanic acid
-
37°C, mutant enzyme M164Q
9.4
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, beta-subunitmutant enzyme E455D
15.1
glutaryl-7-aminocephalosporanic acid
-
37°C, mutant enzyme M164N
16.9
glutaryl-7-aminocephalosporanic acid
-
pH 8.0, 30°C, wild-type enzyme
18.1
glutaryl-7-aminocephalosporanic acid
-
37°C, mutant enzyme M164A
18.6
glutaryl-7-aminocephalosporanic acid
-
37°C, mutant enzyme M164G
49.8
glutaryl-7-aminocephalosporanic acid
-
37°C, wild-type enzyme
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D445G
-
kcat increased, turnover rate improved up to about 10fold compared to the glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase
E423Y/E442Q/D445N
-
kcat/Km is increased, the catalytic efficiency improved up to 1000fold compared to the glutaryl-7-aminocephalosporanic acid acylase activity of gamma-glutamyltranspeptidase
D264A
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
H309A
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
S125A
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
W173A
-
kcat/KM is 2.1fold lower than wild-type value
Y126A
-
kcat/KM is 9.2 fold lower than wild-type value
Y126F
-
kcat/KM is identical to wild-type value
Y57A
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
Y57F
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
D264A
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
-
H309A
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
-
S125A
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
-
Y57A
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
-
Y57F
-
no hydrolysis of glutaryl-7-aminocephalosporanic acid
-
L24F
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 25.9% of the wild-type activity
L24G
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 27% of the wild-type activity, no activity with cephalosporin C
L24K
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 1.1% of the wild-type activity, no activity with cephalosporin C
L24R
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 87.7% of the wild-type activity
L24W
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 11.3% of the wild-type activity, activity with cephalosporin C is 98.8% of wild-type activity
Q50M/F58A
-
beta-subunit mutation, activity with cephalosporin C is 180% of wild-type activity
Q50M/F58D
-
beta-subunit mutation, activity with cephalosporin C is 130% of wild-type activity
Q50M/F58G
-
beta-subunit mutation, activity with cephalosporin C is 290% of wild-type activity
Q50M/F58H
-
beta-subunit mutation, activity with cephalosporin C is 160% of wild-type activity
Q50M/F58I
-
beta-subunit mutation, activity with cephalosporin C is 150% of wild-type activity
Q50M/F58L
-
beta-subunit mutation, activity with cephalosporin C is 120% of wild-type activity
Q50M/F58P
-
beta-subunit mutation, activity with cephalosporin C is 240% of wild-type activity
Q50M/F58S
-
beta-subunit mutation, activity with cephalosporin C is 260% of wild-type activity
Q50M/F58T
-
beta-subunit mutation, activity with cephalosporin C is 230% of wild-type activity
Q50M/Y149F
-
Q50M mutation in beta-subunit, Y149F mutation in alpha-subunit, activity with cephalosporin C is 157% of wild-type activity
Q50M/Y149K
-
Q50M mutation in beta-subunit, Y149K mutation in alpha-subunit, activity with cephalosporin C is 600% of wild-type activity
Q50M/Y149K/F177G
-
beta-subunit mutation, activity with cephalosporin C is 787% of wild-type activity
Q50M/Y149K/F177P
-
beta-subunit mutation, activity with cephalosporin C is 301% of wild-type activity
Q50M/Y149K/F177S
-
beta-subunit mutation, activity with cephalosporin C is 456% of wild-type activity
Q50M/Y149K/M145A
-
Q50M and Y149K are mutation in the beta-subunit, M145A is a mutation in the alpha-subunit, activity with cephalosporin C is 335% of wild-type activity
Q50M/Y149K/M145L
-
Q50M and Y149K are mutations in the beta-subunit, M145L is a mutation in the alpha-subunit, activity with cephalosporin C is 704% of wild-type activity
Q50M/Y149K/M145P
-
Q50M and Y149K are mutations in the beta-subunit, M145P is a mutation in the alpha-subunit, activity with cephalosporin C is 442% of wild-type activity
Q50M/Y149K/M145T
-
Q50M and Y149K are mutation in the beta-subunit, M145T is a mutation in the alpha-subunit, activity with cephalosporin C is 433% of wild-type activity
Q50M/Y149K/Y33N
-
beta-subunit mutation, activity with cephalosporin C is 460% of wild-type activity
Q50M/Y149K/Y33S
-
beta-subunit mutation, activity with cephalosporin C is 568% of wild-type activity
Q50M/Y149K/Y33T
-
beta-subunit mutation, activity with cephalosporin C is 424% of wild-type activity
Q50M/Y33D
-
beta-subunit mutant, activity with cephalosporin C is 450% of wild-type activity
Q50M/Y33G
-
beta-subunit mutant, activity with cephalosporin C is 70% of wild-type activity
Q50M/Y33H
-
beta-subunit mutant, activity with cephalosporin C is 101% of wild-type activity
Q50M/Y33L
-
beta-subunit mutant, activity with cephalosporin C is 202% of wild-type activity
Q50M/Y33N
-
beta-subunit mutant, activity with cephalosporin C is 350% of wild-type activity
Q50M/Y33P
-
beta-subunit mutant, activity with cephalosporin C is 99% of wild-type activity
Q50M/Y33S
-
beta-subunit mutant, activity with cephalosporin C is 60% of wild-type activity
Q50M/Y33T
-
beta-subunit mutant, activity with cephalosporin C is 109% of wild-type activity
Q50M/Y33V
-
beta-subunit mutant, activity with cephalosporin C is 135% of wild-type activity
R155G
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 88% of the wild-type activity
R57S
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
S152G
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 75.4% of the wild-type activity, activity with cephalosporin C is 38.6% of wild-type activity
S152H
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 63.7% of the wild-type activity, activity with cephalosporin C is 11.9% of wild-type activity
S152N
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 78% of the wild-type activity, activity with cephalosporin C is 24.8% of wild-type activity
S152P
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 76.2% of the wild-type activity, activity with cephalosporin C is 60.4% of wild-type activity
S152T
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 81.4% of the wild-type activity, activity with cephalosporin C is 34.7% of wild-type activity
S1betaC
-
no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
S1C
beta-subunit mutant, no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
V70G
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 32.1% of the wild-type activity
V70S
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 28.8% of the wild-type activity, no activity with cephalosporin C
A215E
-
Vmax/Km for cephalosporin C is 1.2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 7.6fold lower than wild-type value
A215F
-
Vmax/Km for cephalosporin C is 2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 3.9fold lower than wild-type value
A215L
-
Vmax/Km for cephalosporin C is 1.3fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 4.2fold lower than wild-type value
A215V
-
Vmax/Km for cephalosporin C is 1.5fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 1.5fold lower than wild-type value
A215Y
-
Vmax/Km for cephalosporin C is 4.3fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 1.6fold lower than wild-type value
A215Y/H296S
-
Vmax/Km for cephalosporin C is 1.2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 25.1fold lower than wild-type value
A215Y/H296S/H309S
-
Vmax/Km for cephalosporin C is 3.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 13.7fold lower than wild-type value
A215Y/H309S
-
Vmax/Km for cephalosporin C is 3.8fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 2.9fold higher than wild-type value
A271F
-
118% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 56.2% of wild-type activity with cephalosporin C as substrate
A271L
-
104% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 100% of wild-type activity with cephalosporin C as substrate
A271Y
-
101% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 122% of wild-type activity with cephalosporin C as substrate
A677A
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme
A677F
site-directed mutagenesis, the mutant shows 24.6% decreased activity compared to the wild-type enzyme
C102S
-
mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C
C199S
-
mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C
C277S
-
mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C
C305S
-
mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C. Expression in Escherichia coli is 2-3fold higher than that of the wild-type enzyme
C391S
-
mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C
C493S
-
mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C
C496S
-
mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C
C748S
-
mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C
D286betaA
beta-subunit mutant enzyme, KM-value is 1.1fold lower than the wild-type value, turnover-number is 1.03fold higher than the wild-type value. Half-life at 37°C is 53.9 h compared to 68.1 h for wild-type enzyme. Optimal pH is pH 9.0 compared to pH 7.0 for wild-type enzyme
D416Y
-
Vmax/Km for cephalosporin C is 1.5fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 5.2fold lower than wild-type value
D416Y/H417Y
-
Vmax/Km for cephalosporin C is 5.3fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 151fold lower than wild-type value
E159D
-
the mutant is still active, but significantly retarded in the second autocleavage compared to the wild type enzyme
E89A/A215Y
-
Vmax/Km for cephalosporin C is 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 25.2fold lower than wild-type value
F229L
turnover number for adipyl-7-aminodesacetoxycephalosporanic acid is nealy identical to wild-type value, 1.8fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.4fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 1.45fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme
F270M
-
Vmax/Km for cephalosporin C is 1.8fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 1.7fold lower than wild-type value
F375M
-
decreased activity compared to the wild type enzyme
G160A
-
the mutant performs the second autocleavage more slowly than the wild type enzyme
G160L
-
the mutant performs the second autocleavage more slowly than the wild type enzyme
H296A
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme
H296F
-
Vmax/Km for cephalosporin C is 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 50.3fold lower than wild-type value
H296N
-
Vmax/Km for cephalosporin C is 1.2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is fold lower than wild-type value
H296S
-
Vmax/Km for cephalosporin C is 1.7fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 10.1fold lower than wild-type value
H296S/H417M
-
Vmax/Km for cephalosporin C is 2fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 151fold lower than wild-type value
H296T
-
Vmax/Km for cephalosporin C is 1.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 5.2fold lower than wild-type value
H309A
site-directed mutagenesis
H309L
site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme
H309Y
-
Vmax/Km for cephalosporin C is 1.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 16.8fold lower than wild-type value
H417Y
-
Vmax/Km for cephalosporin C is more than 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 4.4fold lower than wild-type value
H57betaS/H70betaS/A215alphaY
-
site-directed mutagenesis, the engineered mutant shows increased Vmax on cephalosporin C compared to the wild-type enzyme
H57betaS/H70betaS/F58betaD
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/F58betaI
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/F72betaR
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/I176betaK
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/I176betaQ
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/I176betaT
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/L154betaY
H57betaS/H70betaS/L154betaY/M165alphaS
-
site-directed mutagenesis, the engineered mutant shows increased Vmax on cephalosporin C compared to the wild-type enzyme
H57betaS/H70betaS/L175betaT
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/M165alphaS
-
site-directed mutagenesis, the engineered mutant shows increased Vmax on cephalosporin C compared to the wild-type enzyme
H57betaS/H70betaS/M165alphaS/I176betaS
-
site-directed mutagenesis, the engineered mutant shows increased Vmax on cephalosporin C compared to the wild-type enzyme
H57betaS/H70betaS/R24betaP
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/V25betaR
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
I44V/E49stop/D416Y/H417Y
-
Vmax/Km for cephalosporin C is more than 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is more than 1510fold lower than wild-type value
K100Q
-
81% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 106% of wild-type activity with cephalosporin C as substrate
K114Q
-
86% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 101% of wild-type activity with cephalosporin C as substrate
K170Q
-
130% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 95.6% of wild-type activity with cephalosporin C as substrate
K187Q
-
113% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 91.1% of wild-type activity with cephalosporin C as substrate
K198betaA
beta-subunit mutant enzyme, KM-value is 1.04fold higher than the wild-type value, turnover-number is 1.1fold lower than the wild-type value. Half-life at 37°C is 107.5 h compared to 68.1 h for wild-type enzyme. Optimal pH is pH 8.0 compared to pH 7.0 for wild-type enzyme. Mutant enzyme shows higher stability at alkaline pH than wild-type enzyme
K255Q
-
107% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 97% of wild-type activity with cephalosporin C as substrate
K301Q
-
101% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
K44Q
-
102% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 111% of wild-type activity with cephalosporin C as substrate
K507Q
-
102% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 113.9% of wild-type activity with cephalosporin C as substrate
K629Q
-
94.2% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
K73Q
-
46.9% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 47% of wild-type activity with cephalosporin C as substrate
L677A
the mutant exhibits significantly reduced specific enzymatic activity compared to the wild type enzyme
M116A
-
at pH 7.5, 76.9% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 95.8% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 108% of the specific activity measured with wild-type enzyme with cephalosporin C as substrate
M157A
-
at pH 7.5, 70.9% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 58% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 65% of the specific activity measured with wild-type enzyme with cephalosporin C as substrate. Stability after treatment with H2O2 is lower than that of wild-type enzyme
M164A
-
at pH 7.5, 167% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 104% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 86.6% of the specific activity measured with wild-type enzyme with cephalosporin C as substrate. Stability after treatment with H2O2 is higher than that of wild-type enzyme. The ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 1.2fold lower than the wild-type ratio. The ratio of turnover number to KM-value for glutaryl-7-aminocephalosporanic acid is 2.16fold higher than the wild-type ratio
M164F
-
the ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 1.3fold lower than the wild-type ratio
M164G
-
the ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 1.3fold lower than the wild-type ratio. The ratio of turnover number to KM-value for glutaryl-7-aminocephalosporanic acid is 2.23fold higher than the wild-type ratio
M164L
-
the ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 2.1fold lower than the wild-type ratio
M164N
-
the ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 1.4fold lower than the wild-type ratio. The ratio of turnover number to KM-value for glutaryl-7-aminocephalosporanic acid is 1.81fold higher than the wild-type ratio
M164P
-
the ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 1.3fold lower than the wild-type ratio
M164Q
-
the ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 5.3fold lower than the wild-type ratio. The ratio of turnover number to KM-value for glutaryl-7-aminocephalosporanic acid is nearly identical to the wild-type ratio
M164S
-
the ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 1.5fold lower than the wild-type ratio
M164T
-
the ratio of specific activity with cephalosporin C as substrate to that with glutaryl-7-aminocephalosporanic acid as substrate is 1.4fold lower than the wild-type ratio
M165alphaS/H57betaS/H70betaS
-
site-directed mutagenesis, the engineered mutant shows higher activity on both cephalosporin C and (7R)-7-(4-carboxybutanamido)cephalosporanate compared to the double H57betaS/H70betaS mutant
M174A
-
at pH 7.5, 96.5% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 109% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 122% of the specific activity measured with wild-type enzyme with cephalosporin C as substrate
M227A
-
at pH 7.5, 106% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 74.2% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 105% of the specific activity measured with wild-type enzyme with cephalosporin C as substrate. Stability after treatment with H2O2 is lower than that of wild-type enzyme
M269F
-
98.2% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 165% of wild-type activity with cephalosporin C as substrate. The ratio of turnover number to Km-value with cephalosporin C as substrate is 1.8fold higher than the wild-type ratio
M269L
-
92.5% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 107.9% of wild-type activity with cephalosporin C as substrate
M269Y
-
91.5% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 155% of wild-type activity with cephalosporin C as substrate. The ratio of turnover number to Km-value with cephalosporin C as substrate is 1.6fold higher than the wild-type ratio
M269Y/C305S
-
1.6fold higher activity with cephalosporin C than wild-type enzyme
M270F
site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme
M271V/Q291K/T374S
1.3fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 1.4fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.2fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 1.3fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme
M98A
-
at pH 7.5, 126% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 83.1% of the specific activity measured with wild-type enzyme with glutaryl-7-aminocephalosporanic acid as substrate. At pH 8.7, 91.2% of the specific activity measured with wild-type enzyme with cephalosporin C as substrate
N266M
-
decreased activity compared to the wild type enzyme
N266Q
-
decreased activity compared to the wild type enzyme
N266Q/F375L
-
decreased activity compared to the wild type enzyme
N266S
1.4fold decrease in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 2.3fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.95fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 2.8fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme
Q50betaN/K198betaA
beta-subunit mutant enzyme, KM-value is 2.14fold lower than the wild-type value, turnover-number is 1.45fold higher than the wild-type value, immobilized mutant enzyme shows 34.2% increase in specific activity compared to immobilized wild-type enzyme. Ability of the mutant enzyme to hydrolyze adipoyl 6-aminopenicillinic acid is improved, compared to activity of wild-type enzyme
Q50N
beta-subunit mutant enzyme, KM-value is 2.05fold lower than the wild-type value, turnover-number is 1.45fold higher than the wild-type value. Ability of the mutant enzyme to hydrolyze adipoyl 6-aminopenicillinic acid is improved, compared to activity of wild-type enzyme
R121A
beta-subunit mutant enzyme, KM-value is identical to the wild-type value, turnover-number is nearly identical to wild-type value. Half-life at 37°C is 88.3 h compared to 68.1 h for wild-type enzyme. Optimal pH is pH 6.0 compared to pH 7.0 for wild-type enzyme
R263A
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
R263L
site-directed mutagenesis, the mutant shows decreased activity compared to the wild-type enzyme
S170C
-
the mutant simultaneously loses the activities for both the second autocleavage and substrate hydrolysis
S22P/T394P/D416Y/H417Y
-
Vmax/Km for cephalosporin C is more than 6fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is more than 1510fold lower than wild-type value
S293C
-
1.21% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, no activity with cephalosporin C as substrate
Y151F
alpha-subunit mutant enzyme, KM-value is 1.9fold lower than the wild-type value, turnover-number is 1.17fold higher than the wild-type value. Ability of the mutant enzyme to hydrolyze adipoyl 6-aminopenicillinic acid is improved, compared to activity of wild-type enzyme
Y151F/Q50N
mutant enzyme with mutation Y151F in alpha-subunit and mutation Q50N in beta-subunit, KM-value is 1.2fold lower than the wild-type value, turnover-number is 1.09fold higher than the wild-type value
Y178F/F375H
-
the mutation synergistically improve the catalytic efficiency towards adipyl-7-ADCA 36fold, The activity of this double mutant towards adipyl-7-ADCA is 50% of the activity of the wild-type enzyme towards the preferred substrate glutaryl-7-aminocephalosporanic acid
Y178H
-
decreased activity compared to the wild type enzyme
Y178H/N266H
-
decreased activity compared to the wild type enzyme
Y178H/N266M
-
decreased activity compared to the wild type enzyme
Y178H/N266Q/F375L
-
decreased activity compared to the wild type enzyme
Y215Y/F270S
-
Vmax/Km for cephalosporin C is 2.5fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 1.2fold lower than wild-type value
Y270A
-
70.6% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 24.3% of wild-type activity with cephalosporin C as substrate
Y270E
-
no activity with glutaryl-7-aminocephalosporanic acid or cephalosporin C as substrate
Y270F
-
50.4% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 46.1% of wild-type activity with cephalosporin C as substrate. The ratio of turnover number to Km-value with cephalosporin C as substrate is 2.6fold lower than the wild-type ratio
Y270L
-
28.1% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 32.3% of wild-type activity with cephalosporin C as substrate
Y270S
-
61.7% of wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, 28.3% of wild-type activity with cephalosporin C as substrate
Y271F
-
Vmax/Km for cephalosporin C is 1.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 5.4fold lower than wild-type value
Y27V
-
Vmax/Km for cephalosporin C is 1.5fold lower than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 2.1fold lower than wild-type value
E455D
-
the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 4.5fold lower than the wild-type ratio
E455L
-
the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 34fold lower than the wild-type ratio
E455Q
-
the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 6.1fold lower than the wild-type ratio
H23D
-
the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 9.7fold lower than to the wild-type ratio
H23Q
-
the ratio of turnover number to Km-value of the beta-subunit mutant enzyme is 4.3fold lower than the wild-type ratio
W4A
-
beta-subunit mutant, KM-value for glutaryl-7-aminocephalosporanic acid is 1.6fold higher than wild-type value, turnover number for glutaryl-7-aminocephalosporanic acid is 2.2fold lower than wild-type value, t1/2 at 37°C is 9.8fold lower than wild-type value
W4F
-
beta-subunit mutant, KM-value for glutaryl-7-aminocephalosporanic acid is 1.8fold higher than wild-type value, turnover number for glutaryl-7-aminocephalosporanic acid is 1.6fold lower than wild-type value, t1/2 at 37°C is 37.5fold lower than wild-type value
W4H
-
beta-subunit mutant, KM-value for glutaryl-7-aminocephalosporanic acid is 1.9fold higher than wild-type value, turnover number for glutaryl-7-aminocephalosporanic acid is 1.4fold lower than wild-type value, t1/2 at 37°C is 1.9fold lower than wild-type value
W4L
-
beta-subunit mutant, KM-value for glutaryl-7-aminocephalosporanic acid is 1.8fold higher than wild-type value, turnover number for glutaryl-7-aminocephalosporanic acid is 2.1fold lower than wild-type value, t1/2 at 37°C is 6.1fold lower than wild-type value
W4T
-
beta-subunit mutant, KM-value for glutaryl-7-aminocephalosporanic acid is 1.7fold higher than wild-type value, turnover number for glutaryl-7-aminocephalosporanic acid is 2.4fold lower than wild-type value, t1/2 at 37°C is 50fold lower than wild-type value
W4Y
-
beta-subunit mutant, KM-value for glutaryl-7-aminocephalosporanic acid is 1.6fold higher than wild-type value, turnover number for glutaryl-7-aminocephalosporanic acid is 1.3fold lower than wild-type value, t1/2 at 37°C is 12fold lower than wild-type value
F58betaN/H70betaS/I176betaT
site-directed mutagenesis, mutation of wild-type variant M31betaF, comparison of substrate binding structures and abilities with the wild-type, molecular dynamics simulations and modeling, overview
H57betaS/H70betaS/F72betaR
site-directed mutagenesis, the mutant enzyme shows increased activity on cephalosporin compared to the wild-type enzyme, this mutant is the most active acylase on glutaryl-7-aminocephalosporanic acid
H57betaS/H70betaS/L154betaY
site-directed mutagenesis, the mutant enzyme shows increased activity on cephalosporin compared to the wild-type enzyme
L154betaF
site-directed mutagenesis, mutant M8, the mutant displays improved stability and activity compared to the wild-type
L154betaF/L180betaF
site-directed mutagenesis, mutant M10, the mutant displays improved stability and activity compared to the wild-type
L154betaF/Y167betaF/L180betaF
site-directed mutagenesis, the mutant displays improved stability and activity compared to the wild-type
L180betaF
site-directed mutagenesis, mutant M9, the mutant displays improved stability and activity compared to the wild-type
M31betaF/H57betaS/H70betaS
site-directed mutagenesis, mutant M1, the mutant displays improved stability and activity compared to the mutant M2
M31betaF/H57betaS/V68betaA/H70betaS
site-directed mutagenesis, mutant M2, the mutant shows a decrease in stability compared to wild-type, which is largely owing to the mutation V68betaA at the active site
M31betaF/H57betaS/V68betaA/H70betaS/L154betaF/L180betaF
site-directed mutagenesis
Y167betaF
site-directed mutagenesis, the mutant displays improved stability and activity compared to the wild-type
C471betaS
site-directed mutagenesis, mutant M3, mutation within the hydrophobic core regions of the alphabetabetaalpha structural motif
C471betaS/A114alphaS
site-directed mutagenesis, mutant M10, the mutant shows altered catalytic efficiency compared to wild-type
C471betaS/A114alphaS/A38betaS
site-directed mutagenesis, mutant M12
C471betaS/A114alphaS/A38betaS/L180betaF
site-directed mutagenesis, mutant M13, the mutant shows altered catalytic efficiency compared to wild-type
C471betaS/A38betaS/L154betaF
site-directed mutagenesis, mutant M14, the mutant shows altered catalytic efficiency compared to wild-type
C471betaS/A38betaS/L154betaF/L180betaF
site-directed mutagenesis, mutant M15, the mutant shows altered catalytic efficiency compared to wild-type
C47C471betaS/A38betaS
site-directed mutagenesis, mutant M11, the mutant shows altered catalytic efficiency compared to wild-type
L154betaF
site-directed mutagenesis, mutant M4, mutation within the hydrophobic core regions of the alphabetabetaalpha structural motif
L180betaF
site-directed mutagenesis, mutant M5, mutation within the hydrophobic core regions of the alphabetabetaalpha structural motif
N3betaT
site-directed mutagenesis, mutant M1, mutation within the hydrophobic core regions of the alphabetabetaalpha structural motif
N3betaV
site-directed mutagenesis, mutant M2, mutation within the hydrophobic core regions of the alphabetabetaalpha structural motif
F229L
-
turnover number for adipyl-7-aminodesacetoxycephalosporanic acid is nealy identical to wild-type value, 1.8fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.4fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 1.45fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme
-
F375M
-
decreased activity compared to the wild type enzyme
-
M271V/Q291K/T374S
-
1.3fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 1.4fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.2fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 1.3fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme
-
N266M
-
decreased activity compared to the wild type enzyme
-
N266Q
-
decreased activity compared to the wild type enzyme
-
N266S
-
1.4fold decrease in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 2.3fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.95fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 2.8fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme
-
F177betaG
site-directed mutagenesis
F177betaG/M145alphaA/Y149alphaV
site-directed mutagenesis, mutant CAAC2, the mutant has an altered substrate binding site with increased affinity for binding of long acyl chains, it shows altered substrate specificity, lower enzymatic activity with cephalosporin but higher activity with aculeacin A, in comparison with the wild-type enzyme
F375A
-
turnover number is 2.45fold lower than wild-type value, KM-value is 1.9fold lower than wild-type value, turnover number is 1.7fold lower than wild-type value, KM-value is 2.2fold higher than wild-type value
F375C
-
turnover number is 1.1fold higher than wild-type value, KM-value is 5.9fold lower than wild-type value, turnover number is 1.7fold lower than wild-type value, KM-value is 1.2fold higher than wild-type value
F375D
-
turnover number is 32.7fold lower than wild-type value, KM-value is 11fold higher than wild-type value, turnover number is 400fold lower than wild-type value, KM-value is 7fold higher than wild-type value
F375E
-
turnover number is 6.1fold lower than wild-type value, KM-value is 12fold higher than wild-type value, turnover number is 500fold lower than wild-type value, KM-value is 6.7fold higher than wild-type value
F375G
-
turnover number is 6.7fold lower than wild-type value, KM-value is 6.25fold lower than wild-type value, turnover number is 1.6fold lower than wild-type value, KM-value is 2.1fold higher than wild-type value
F375H
-
turnover number is 2.4fold higher than wild-type value, KM-value is 1.1fold lower than wild-type value, turnover number is 3fold lower than wild-type value, KM-value is 3.5fold higher than wild-type value
F375I
-
turnover number is 5fold lower than wild-type value, KM-value is 2.1fold higher than wild-type value, turnover number is 6fold lower than wild-type value, KM-value is 3.2fold higher than wild-type value
F375K
-
turnover number is 13.6fold lower than wild-type value, KM-value is 2.2fold higher than wild-type value, turnover number is 1.8fold lower than wild-type value, KM-value is 22.6fold higher than wild-type value
F375L
-
turnover number is 1.4fold higher than wild-type value, KM-value is 1.4fold lower than wild-type value, turnover number is 3.6fold lower than wild-type value, KM-value is 8.4fold higher than wild-type value
F375M
-
turnover number is 1.3fold higher than wild-type value, KM-value is 1.5fold lower than wild-type value, turnover number is 2fold lower than wild-type value, KM-value is 5.5fold higher than wild-type value
F375N
-
turnover number is 1.8fold higher than wild-type value, KM-value is 1.2fold lower than wild-type value, turnover number is 2fold lower than wild-type value, KM-value is 5.8fold higher than wild-type value
F375Q
-
turnover number is 1.4fold higher than wild-type value, KM-value is 2.1fold higher than wild-type value, turnover number is 5fold lower than wild-type value, KM-value is 22.6fold higher than wild-type value
F375R
-
turnover number is 27.2fold lower than wild-type value, KM-value is 10fold higher than wild-type value, turnover number is 19fold lower than wild-type value, KM-value is 19fold higher than wild-type value
F375S
-
turnover number is 4.5fold lower than wild-type value, KM-value is nearly identical to wild-type value, turnover number is 1.3fold lower than wild-type value, KM-value is 2.3fold higher than wild-type value
F375T
-
turnover number is 2fold lower than wild-type value, KM-value is 1.1fold lower than wild-type value, turnover number is 2.2fold lower than wild-type value, KM-value is 2.9fold higher than wild-type value
F375V
-
turnover number is 5.4fold lower than wild-type value, KM-value is 1.4fold higher than wild-type value, turnover number is 3.9fold lower than wild-type value, KM-value is 3.2fold higher than wild-type value
F375W
-
turnover number is 18.8fold lower than wild-type value, KM-value is 1.5fold higher than wild-type value, turnover number is 8.5fold lower than wild-type value, KM-value is 25.8fold higher than wild-type value
F375Y
-
turnover number is 1.14fold lower than wild-type value, KM-value is 2.4fold lower than wild-type value, turnover number is 1.1fold lower than wild-type value, KM-value is 2.6fold higher than wild-type value
N266A
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 2.3fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.9fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 8.4fold higher than wild-type value
N266C
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.3fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.5fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.2fold higher than wild-type value
N266D
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 205fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold higher than wild-type value. Less efficient cleavage of the spacer from the alpha-subunit compared to wild-type enzyme
N266E
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 17.8fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 11.5fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 160fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 9.4fold higher than wild-type value. Less efficient cleavage of the spacer from the alpha-subunit compared to wild-type enzyme
N266F
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.1fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 4.4fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.2fold higher than wild-type value
N266G
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 17.4fold higher than wild-type value
N266H
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.15fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 8.6fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 1.3fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 1.4fold higher than wild-type value. 100% improved conversion of cephalosporin C compared to wild type enzyme
N266L
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.64fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 48.8fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 13.9fold higher than wild-type value
N266M
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 4.6fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 3.6fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.4fold higher than wild-type value
N266P
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 6.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 3.7fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 30.8fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 41.9fold higher than wild-type value
N266Q
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.12fold higher than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.9fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 1.7fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.9fold higher wild-type value. 100% improved conversion of cephalosporin C compared to wild type enzyme
N266S
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 2.5fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 2.4fold higher than wild-type value
N266T
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 2.7fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 4.75fold higher than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 8.2fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 48.4fold higher than wild-type value
N266W
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 2fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 3fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 5.8fold higher than wild-type value. 30% improved conversion of cephalosporin C compared to wild type enzyme
N266Y
-
turnover number for adipyl-7-aminodeacetoxycephalosporanic acid is 1.2fold lower than wild-type value, KM-value for adipyl-7-aminodeacetoxycephalosporanic acid is 1.8fold lower than wild-type value, turnover-number for glutaryl-7-aminocephalosporanic acid is 6.6fold lower than wild-type value, KM-value for glutaryl-7-aminocephalosporanic acid is 3.5fold higher than wild-type value
F177H
beta-subunit mutant, 17.6% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
F177H
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 17.6% of the wild-type activity, no activity with cephalosporin C
F177P
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
F177P
beta-subunit mutant, only partial intramolecular cleavage and no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
F177T
beta-subunit mutant, 4.3% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate. Only partial intermolecular cleavage in posttranslational modification
F177T
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 4.3% of the wild-type activity, no activity with cephalosporin C
Q50L
beta-subunit mutant, 9.9% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50L
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 9.9% of the wild-type activity, no activity with cephalosporin C
Q50M
beta-subunit mutant, 7.5% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50M
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 7.5% of the wild-type activity, activity with cephalosporin C is 180% of wild-type activity
Q50R
beta-subunit mutant, 2.1% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50R
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 2.1% of the wild-type activity, no activity with cephalosporin C
Q50T
beta-subunit mutant, 72.5% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50T
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 72.5% of the wild-type activity, no activity with cephalosporin C
Q50Y
beta-subunit mutant, 15.7% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Q50Y
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 15.7% of the wild-type activity, no activity with cephalosporin C
R57C
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
R57C
beta-subunit mutant, no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
R57I
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
R57I
beta-subunit mutant, no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
R57K
beta-subunit mutant, 0.3% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate, only partial intermolecular cleavage in posttranslational modification
R57K
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 0.3% of the wild-type activity, no activity with cephalosporin C
S1A
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid and cephalosporin C
S1A
beta-subunit mutant, no processing takes place, no activity with glutaryl-7-aminocephalosporanic acid
Y149C
alpha-subunit mutant, 16.4% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149C
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 16.4% of the wild-type activity, activity with cephalosporin C is 24% of wild-type activity
Y149G
alpha-subunit mutant, 0.7% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149G
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 0.7% of the wild-type activity, no activity with cephalosporin C
Y149L
alpha-subunit mutant, 6.3% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149L
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 6.3% of the wild-type activity, activity with cephalosporin C is 48% of wild-type activity
Y149N
alpha-subunit mutant, 30.8% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149N
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 30.8% of the wild-type activity, activity with cephalosporin C is 48% of wild-type activity
Y149P
alpha-subunit mutant, 1.1% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149P
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 1.1% of the wild-type activity, no activity with cephalosporin C
Y149R
alpha-subunit mutant, 13.6% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y149R
-
alpha-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 13.6% of the wild-type activity, no activity with cephalosporin C
Y33F
beta-subunit mutant, 65.4% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate
Y33F
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 65.4% of the wild-type activity, no activity with cephalosporin C
Y33I
beta-subunit mutant, 9.3% of the wild-type activity with glutaryl-7-aminocephalosporanic acid as substrate only partial intermolecular cleavage in posttranslational modification
Y33I
-
beta-subunit mutant, activity with glutaryl-7-aminocephalosporanic acid is 9.3% of the wild-type activity, activity with cephalosporin C is 90% of wild-type activity
Y33S
-
beta-subunit mutant, no activity with glutaryl-7-aminocephalosporanic acid, activity with cephalosporin C is 120% of the wild-type activity
Y33S
beta-subunit mutant, no intermolecular cleavage in posttranslational modification, no activity with glutaryl-7-aminocephalosporanic acid
A675G
the mutant exhibits 112.8% activity with cephalosporin C compared to the wild type enzyme
A675G
the mutant exhibits significantly enhanced specific enzymatic activity compared to the wild type enzyme (35% increase)
A675G
site-directed mutagenesis, the mutant shows 13% increased activity compared to the wild-type enzyme
F375L
1.5fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 1.2fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 3.9fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 8.2fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme. Improved activity ratio for adipyl-7-aminodesacetoxycephalosporanic acid to glutaryl-7-aminocephalosporanic acid of the mutant enzyme is a consequence of a decreased catalytic efficiency towards glutaryl-7-aminocephalosporanic acid
F375L
-
decreased activity compared to the wild type enzyme
H296S/H309S
-
Vmax/Km for cephalosporin C is 4fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 21.6fold lower than wild-type value
H296S/H309S
-
the mutant also shows activity with cephalosporin C (3fold increase compared to the wild type enzyme)
H309S
-
Vmax/Km for cephalosporin C is 1.5fold higher than wild-type value, Vmax/Km for glutaryl-7-amino cephalosporanic acid is 8.9fold lower than wild-type value
H309S
site-directed mutagenesis, the mutant shows slightly decreased activity compared to the wild-type enzyme
H309V
the mutant exhibits 88.9% activity with cephalosporin C compared to the wild type enzyme
H309V
site-directed mutagenesis, the mutant shows 11.1% decreased activity compared to the wild-type enzyme
H57betaS/H70betaS
-
site-directed mutagenesis, interest in designing a single step enzymatic conversion of cephalosporin C to (7R)-7-aminocephalosporanate catalyzed by a true cephalosporin C acylase, the engineered mutant displays enhanced catalytic efficiency on cephalosporin C over glutaryl-7-aminocephalosporanic acid compared to the wild-type enzyme. The nucleophilic catalytic serine residue, Ser1beta, is situated at the base of the active site cavity
H57betaS/H70betaS
-
site-directed mutagenesis, the engineered mutant shows increased Vmax on cephalosporin C compared to the wild-type enzyme
H57betaS/H70betaS/L154betaY
-
random mutagenesis of mutant H57betaS/H70betaS, the mutant shows altered substrate specificity compared to the wild-type enzyme
H57betaS/H70betaS/L154betaY
-
site-directed mutagenesis, interest in designing a single step enzymatic conversion of cephalosporin C to (7R)-7-aminocephalosporanate catalyzed by a true cephalosporin C acylase, the engineered mutant displays highly enhanced catalytic efficiency, with a 11000fold increase in specificity constant for CephC versus glutaryl-7-amino cephalosporanic acid, on cephalosporin C over glutaryl-7-aminocephalosporanic acid compared to the wild-type enzyme under conditions resembling those used at industrial level because of its high kinetic efficiency and the absence of substrate or product inhibition effects
L666F
the mutant exhibits significantly reduced specific enzymatic activity (75.4%) with cephalosporin C compared to the wild type enzyme
L666F
site-directed mutagenesis, the mutant shows highly decreased activity compared to the wild-type enzyme
N266H
1.2fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 6.9fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.3fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 2fold decrease in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme. Nearly 10fold improved catalytic efficiency on adipyl-7-aminodesacetoxycephalosporanic acid, resulting from a 50% increase in turnover-number and a 6fold decrease in KM-value without decreasing the catalytic efficiency on glutaryl-7-aminocephalosporanic acid
N266H
-
decreased activity compared to the wild type enzyme
P295A
the mutant exhibits 20% activity with cephalosporin C compared to the wild type enzyme
P295A
site-directed mutagenesis, the mutant shows 80% decreased activity compared to the wild-type enzyme
H57betaS/H70betaS
naturally occuring mutations, the two mutations increase the activity by fourfold. Structure-activity modelling using wild-type and mutant enzymes, overview. Design of N176 cephalosporin acylase at active site and structure model of predicted mutation V68betaA
H57betaS/H70betaS
site-directed mutagenesis, the mutant enzyme shows increased activity on cephalosporin compared to the wild-type enzyme
F375L
-
decreased activity compared to the wild type enzyme
-
F375L
-
1.5fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 1.2fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 3.9fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 8.2fold increase in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme. Improved activity ratio for adipyl-7-aminodesacetoxycephalosporanic acid to glutaryl-7-aminocephalosporanic acid of the mutant enzyme is a consequence of a decreased catalytic efficiency towards glutaryl-7-aminocephalosporanic acid
-
N266H
-
decreased activity compared to the wild type enzyme
-
N266H
-
1.2fold increase in turnover number for adipyl-7-aminodesacetoxycephalosporanic acid, 6.9fold decrease in KM-value for adipyl-7-aminodesacetoxycephalosporanic acid, 1.3fold decrease in turnover number for glutaryl-7-aminocephalosporanic acid, 2fold decrease in Km-value for glutaryl-7-aminocephalosporanic acid as compared to wild-type enzyme. Nearly 10fold improved catalytic efficiency on adipyl-7-aminodesacetoxycephalosporanic acid, resulting from a 50% increase in turnover-number and a 6fold decrease in KM-value without decreasing the catalytic efficiency on glutaryl-7-aminocephalosporanic acid
-
F177betaG/M145alphaA
site-directed mutagenesis, mutant CAAC1, the mutant has an altered substrate binding site with increased affinity for binding of long acyl chains, it shows altered substrate specificity, lower enzymatic activity with cephalosporin but higher activity with aculeacin A, in comparison with the wild-type enzyme
F177betaG/M145alphaA
site-directed mutagenesis, mutant CAAC2, the mutant has an altered substrate binding site with increased affinity for binding of long acyl chains, it shows altered substrate specificity, lower enzymatic activity with cephalosporin but higher activity with aculeacin A, in comparison with the wild-type enzyme
additional information
-
construction of 14 mutants with deletion in the alpha-C-terminal region, the majority of the fragment-deleted mutant proteins completely lose their activity due to failure of the first autocleavage, while mutant proteins D2 (227-AM-228 deletion) and D4 (212-ADLA-215 deletion) formally activate into mature enzyme with high activity. The Kcat/Kmvalues of mutant proteins D2 and D4 are 46% and 102% higher than that of wild-type control, respectively
additional information
-
mutant screening of H57betaS/H70betaS triple mutants generated by site-saturation mutagenesis, introduction of multiple mutations, overview
additional information
the enzyme is mutated by PCR method to add a 3G3K tag to the C-terminal of the beta-subunit, the wild-type and mutated enzyme have nearly equal specific activity. The mutant enzyme has a 20% higher expressed activity than its wild-type counterpart. Immobilization of mutated and wild-type enzyme on glyoxyl agarose support via surface lysine or the poly-lysine tag, respectively
additional information
-
three-dimensional structure model of the wild-type enzyme for semi-rational determination of mutation residues, overview
additional information
three-dimensional structure model of the wild-type enzyme for semi-rational determination of mutation residues, overview
additional information
proton-producing enzyme cephalosporin C acylase (CCA) is covalently bound on an epoxy-activated porous support. The microenvironmental pH change in immobilized CCA during the reaction is detected using pH-sensitive fluorescein labeling (pH-sensitive fluorescein molecule co-immobilized on the carrier). The high catalytic velocity of the initial stage of conversion results in a sharp intraparticle pH gradient, which is likely the key factor relating to low operational stability. Mass transfer in the immobilized CCA is relatively severe during CPC hydrolysis. Reducing the intraparticle pH gradient, i.e. diminishing the diffusion limitation, is important for the catalysis of CCA. Accordingly, another strategy for a two-stage catalytic process is developed to reduce the reaction rate of stage I at a low temperature to preserve enzymatic activity and to shorten the duration of catalysis at a high reaction temperature in stage II. The reaction using the two-stage catalytic process (10-37°C shift at 30 min) shows significantly improved stability compared with that of the single-temperature reaction at 37°C (29 batches versus five batches, respectively) and a shorter catalytic period than the reaction at 10°C (40 min versus 70 min, respectively)
additional information
the purified recombinant His-tagged enzyme is immobilized on different supports, i.e. an epoxy-activated support LX1000-EPC4 (EP) or its derivatives, EP-polyethyleneimine (EP-PEI) and EP-ethylenediamine (EP-EDA) with cationic groups on the surface, validation and optimization. The CCA immobilized on EP-PEI has the best stability and its activity declines very slowly at 45°C. The enzyme immobilized on the cationic support EP-EDA has a half-life of 100 min at 45°C and pH 8.0, a value much lower than on EP-PEI. Stabilization factors of enzyme immobilized on EP-PEI and EP-EDA are 35.6fold and 5.5fold, respectively. The Km values of CCA immobilized on EP-PEI and EP-EDA are even lower than that of the free enzyme, indicating that a higher substrate affinity is obtained after the immobilization on the cationic supports. The reusability of the EP-PEI support significantly reduces the cost of the biocatalyst preparation
additional information
-
deletion of the signal peptide and mutation in the alpha-subunit of the acylase has little influence on its posttranslational processing and its kinetic parameters
additional information
substitution of the first residue of the beta-subunit, Ser, results in a complete loss of enzyme activity, and substitution of the last residue of the spacer, Gly, leads to an inactive and unprocessed precursor
additional information
cephalosporin C acylase has the potential to transform cephalosporin C to 7-aminocephalosporanic acid which is an important intermediate for production of broad-spectrum semisynthetic cephalosporins. Selection of medium components and parameters like temperature, pH, carbon sources, nitrogen sources, and inoculum level for the optimal production of cephalosporin C acylase by Pseudomonas species, overview. The biotransformation is conducted utilizing all optimized parameters for the production of cephalosporin C acylase. The maximum CCA acylase activity is detected at 72 h of incubation. The highest CCA activity for Pseudomonas species of 42.9 U/ml is detected at 72 h of incubation at pH 8.0 and 30°C
additional information
mutation V68betaA at the active site causes a loss in protein stability. The lost stability because is recovered by introducing mutations L154betaF and L180betaF at hydrophobic core regions. Mutation V68betaA in the six-residue mutant provides more space to accommodate the bulky side chain of cephalosporin C, which can help in designing cephalosporin C acylase mutants with higher activities and the practical one-step enzymatic route to prepare 7-aminocephalosporanic acid at industrial-scale levels
additional information
one-pot conversion of cephalosporin C by using an optimized two-enzyme process, producing 7-aminocephalosporanic acid (7-ACA) and involving D-amino acid oxidase (DAAO, EC 1.4.3.3) and glutaryl-7-aminocephalosporanic acid acylase (GA, EC 3.5.1.93). Stability of the employed biocatalysts when incubated at 25°C in 20 mM phosphate buffer, pH 8.0, overview. The two enzymes are used in one-pot and in a soluble form. By adding 4.5 kU/l of both RgDAAO and wild-type VAC, 50 mM cephalosporin C is fully converted in about 7 hours although only 30 mM 7-ACA is produced
additional information
optimization of enzyme protein engineering for biocatalytic production of cephalosporins, molecular dynamics simulations with wild-type enzyme and mutant M6, analysis of access of the substrate cephalosporin C from the bulk to the active site and stability of the enzyme-substrate complex. In both variants, cephalosporin C is binding to a non-productive substrate binding site (E86alpha, S369beta, S460beta) at the entrance to the binding pocket, preventing substrate access. A second non-productive binding site (G372beta, W376beta, L457beta) is identified within the binding pocket, which competes with the active site for substrate binding
additional information
computational design of thermostable mutants for cephalosporin C acylase from Pseudomonas strain SE83, overview. Redesign to enhance its stability by repacking the hydrophobic core regions and reconstructing the protein-protein interactions in the segment interface regions. Single point mutations Asn2betaThr, Asn2betaVal, Cys470betaSer, Leu154betaPhe, and Leu180betaPhe in hydrophobic core regions, and Ala100alphaSer and Ala37betaSer in segment-segment interface regions, increase the Tm by 4.7-19.7°C, while combining these confirmed single mutations increases the Tm by up to 20.5°C. Construction of six multiple-point variants with negative calculated folding free energy changes. At a reaction temperature of 37°C, the catalytic efficiencies of the design template, and mutants M1, M11, and M13 are 2.72, 2.48, 0.42, and 0.64 U/mg/ mM, respectively. At 50°C, the catalytic efficiencies are 0.475, 0.70, 0.80, and 0.92 U/mg/mM, respectively
additional information
in a stirred tank reactor, during catalysis with immobilized cephalosporin C acylase (CCA), the microenvironmental pH drops to pH 7.2 in a nonbuffered system (with the pH maintained at pH 8.5 by adding alkali) due to the existence of diffusional resistance. The immobilized CCA only catalyzes only five batch reactions, suggesting that the sharp pH gradient impairs the enzyme stability. To buffer the protons produced in the hydrolysis of cephalosporin C by CCA, phosphate and bicarbonate buffers are introduced. When CCA is catalyzed with 0.1 M ammonium bicarbonate buffer, no obvious gradient between the bulk solution and intraparticle pH is detected, and the catalysis of 15 batch reactions is achieved. Accordingly, with 0.2 M ammonium bicarbonate buffer in a packed bed reactor, the immobilized CCA exhibits continuous catalysis with high conversion rates (over 95%) for 21 days. Reactions with ammonium bicarbonate buffer shows significant increases in the stability and catalytic efficiency of the immobilized CCA in different reactors compared to those in nonbuffered systems. Set-up for measurement of internal pH at different positions in the packed-bed reactor, overview
additional information
the recombinant enzyme is immobilized, microenvironment with an acidic pH (about pH 6-7) during the catalysis of cephalosporin C with the immobilized enzyme. The immobilization efficiency is increased when the enzyme is immobilized in the presence of inhibitors sodium acetate and sodium bicarbobate. And the immobilized enzyme, in the presence of inhibitors, has a higher thermostability than the control at 50°C
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