EC Number   |
General Stability |
Reference |
|---|
   2.7.3.2 | 2-mercaptoethanol enhances pH-stability |
642407 |
| 2.7.3.2 | a His6-tag portion has no effect on the enzyme activity |
761260 |
| 2.7.3.2 | dimeric enzyme stable to 1-2 M urea |
642376 |
| 2.7.3.2 | in the presence of cryoprotectant (sucrose or trehalose), the activities of wild-type and mutant M1-CK enzymes with a hydrophobic residue at 268 are higher, and the effect is more profound at pH 8.0 |
739256 |
| 2.7.3.2 | in the presence of cryoprotectant (sucrose or trehalose), the activities of wild-type and mutant RM-CK enzymes with a hydrophobic residue at 268 are higher, and the effect is more profound at pH 8.0 |
739256 |
| 2.7.3.2 | isozyme CK-MM is more stable against unfolding by urea up to 3 M, while isozymes CK-BB and CK-MB unfold at lower urea concentrations of 2 M, at 25°C and pH 8.0 |
660931 |
| 2.7.3.2 | polyethylene glycol 2000 (PEG 2000) and dextran 70 are used as model crowding agents to examine the effects of macromolecular crowding on the inactivation of recombinant HBCK (rHBCK) during denaturation by GdnHCl. Both PEG 2000 and dextran 70 have a protective effect on the inactivation of rHBCK induced by GdnHCl at 25°C. The presence of PEG 2000 results in the retention of 35.33% of rHBCK activity after 4 h of inactivation, while no rHBCK activity is observed after denaturation in the absence of macromolecular crowding agents. The presence of PEG 2000 and dextran 70 at a concentration of 100 g/l can decelerate the k2 value of the slow track to 21 and 33%, respectively, in comparison to values obtained in the absence of crowding agents |
721310 |
| 2.7.3.2 | sensitive to denaturation |
642396 |
| 2.7.3.2 | the activity of the 3-(4-chloro-6-p-glyoxal-phenoxy-1,3,5-triazinylamino)-7-(dimethylamino)-2-methylphenazine-labeled creatine kinase is found to be about 40% of that of the native creatine kinase, which supports that the modified arginine residue by 3-(4-chloro-6-p-glyoxal-phenoxy-1,3,5-triazinylamino)-7-(dimethylamino)-2-methylphenazine is at the active site |
702414 |
| 2.7.3.2 | when incubated with 0.8 M guanidine hydrochloride, MM-CK accumulates as a monomeric molten globule which totally lost its enzymatic activity |
671618 |
