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Literature summary for 2.7.3.2 extracted from
- The activity of carp muscle-specific creatine kinase at low temperature is enhanced by decreased hydrophobicity of residue 268 (2014), Physiol. Biochem. Zool., 87, 507-516.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G268D | site-directed mutagenesis, the mutant shows increased activity at 10°C and pH 8.0 compared to the wild-type | Oryctolagus cuniculus |
| G268D | site-directed mutagenesis, the mutant shows increased activity at 10°C and pH 8.0 compared to the wild-type | Cyprinus carpio |
| G268K | site-directed mutagenesis, the mutant shows increased activity at 10°C and pH 8.0 compared to the wild-type | Oryctolagus cuniculus |
| G268K | site-directed mutagenesis, the mutant shows increased activity at 10°C and pH 8.0 compared to the wild-type | Cyprinus carpio |
| G268L | site-directed mutagenesis, the mutant does not show altered activity at 10°C and pH 8.0 compared to the wild-type | Oryctolagus cuniculus |
| G268L | site-directed mutagenesis, the mutant does not show altered activity at 10°C and pH 8.0 compared to the wild-type | Cyprinus carpio |
| G268N | site-directed mutagenesis, the mutant shows increased activity at 10°C compared to the wild-type | Oryctolagus cuniculus |
General Stability
| General Stability | Organism |
|---|---|
| in the presence of cryoprotectant (sucrose or trehalose), the activities of wild-type and mutant M1-CK enzymes with a hydrophobic residue at 268 are higher, and the effect is more profound at pH 8.0 | Cyprinus carpio |
| in the presence of cryoprotectant (sucrose or trehalose), the activities of wild-type and mutant RM-CK enzymes with a hydrophobic residue at 268 are higher, and the effect is more profound at pH 8.0 | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyprinus carpio | Q9YI16 | - |
- |
| Oryctolagus cuniculus | P00563 | gene CKM | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Oryctolagus cuniculus | - |
| skeletal muscle | - |
Cyprinus carpio | - |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| M1-CK | - |
Cyprinus carpio |
| RM-CK | - |
Oryctolagus cuniculus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the hydrophobicity of enzyme residue 268 affects the biochemical properties of enzyme M1-CK at low temperatures | Cyprinus carpio |
| additional information | - |
the hydrophobicity of enzyme residue 268 affects the biochemical properties of enzyme RM-CK at low temperatures | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Cyprinus carpio |
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Release 2023.1 (January 2023)
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