Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ET8894 | Phaseolus vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
E297A | Km for ammonium strongly increased, minor effects on activity in forward reaction, reverse reaction activity strongly decreased, strong effect on binding behavior to a 2',5'-ADP-sepharose | Phaseolus vulgaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-methionine sulfoximine | forward reaction: complete inhibition of wild type enzyme, only slight inhibition of mutant enzyme, reverse reaction: slight inhibition of mutant and wild-type enzyme | Phaseolus vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ATP | wild-type and mutant enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
2 | - |
L-glutamate | mutant enzyme, pH 7.5, 37°C | Phaseolus vulgaris | |
7 | - |
L-glutamate | wild-type enzyme, pH 7.5, 37°C | Phaseolus vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
SDS-PAGE | Phaseolus vulgaris |
350000 | - |
gel filtration | Phaseolus vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glutamate + NH4+ | Phaseolus vulgaris | central enzyme of nitrogen metabolism, ammonia assimilation | ADP + phosphate + L-glutamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaseolus vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant and wild-type enzyme | Phaseolus vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glutamate + NH4+ | - |
Phaseolus vulgaris | ADP + phosphate + L-glutamine | - |
r | |
ATP + glutamate + NH4+ | central enzyme of nitrogen metabolism, ammonia assimilation | Phaseolus vulgaris | ADP + phosphate + L-glutamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 43000, subunits by SDS-PAGE, native mass by gel filtration | Phaseolus vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
forward reaction of wild-type and mutant enzyme, reverse reaction of wild-type enzyme | Phaseolus vulgaris |
45 | - |
reverse reaction of mutant enzyme | Phaseolus vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
wild-type and mutant enzyme, forward and reverse reaction | Phaseolus vulgaris |