Information on EC 6.3.1.2 - glutamate-ammonia ligase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
6.3.1.2
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RECOMMENDED NAME
GeneOntology No.
glutamate-ammonia ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid amide hydrolysis
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carboxylic
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Alanine, aspartate and glutamate metabolism
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ammonia assimilation cycle I
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ammonia assimilation cycle II
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Arginine biosynthesis
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glutamate and glutamine metabolism
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Glyoxylate and dicarboxylate metabolism
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L-glutamine biosynthesis I
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L-glutamine biosynthesis III
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Metabolic pathways
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Microbial metabolism in diverse environments
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nitrate reduction II (assimilatory)
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nitrate reduction V (assimilatory)
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nitrate reduction VI (assimilatory)
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Nitrogen metabolism
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SYSTEMATIC NAME
IUBMB Comments
L-glutamate:ammonia ligase (ADP-forming)
Also acts, more slowly, on 4-methylene-L-glutamate (cf. EC 6.3.1.7 4-methyleneglutamate---ammonia ligase).
CAS REGISTRY NUMBER
COMMENTARY hide
9023-70-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
spiny mouse
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain U32
UniProt
Manually annotated by BRENDA team
-
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-
Manually annotated by BRENDA team
Anabaena sp. PCC 7120
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-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
Azolla sp.
a genus of aquatic ferns, which harbors the cyanobacterium Anabaena azollae in specialized cavities formed in its leaves
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
isozyme GSI-alpha
UniProt
Manually annotated by BRENDA team
strain KN2
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Manually annotated by BRENDA team
isozyme GS2
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-
Manually annotated by BRENDA team
strain a
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-
Manually annotated by BRENDA team
strain 203
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Manually annotated by BRENDA team
subspecies animalis a, subspecies animalis b
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Manually annotated by BRENDA team
subspecies infantis a, subspecies liberorum
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Manually annotated by BRENDA team
strain B
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Manually annotated by BRENDA team
strain B
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
oxygen-resistant mutant strain, isoforms GSI and GSII
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain NCIB 10338
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Manually annotated by BRENDA team
strain NCIB 10338
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Manually annotated by BRENDA team
gene glnA1 or cg2429 encoding a GSI-beta-subtype enzyme GlnA1 as the functional enzyme form in the organism, gene glnA2 encodes a GSI-alpha-subtype
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
i.e. Spiniraja whitleyi
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Manually annotated by BRENDA team
a highly regenerative oligochaete annelid, gene gs
UniProt
Manually annotated by BRENDA team
strain CpI1, 2 enzyme forms GSI and GSII
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Manually annotated by BRENDA team
strain CpI1, 2 enzyme forms GSI and GSII
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Manually annotated by BRENDA team
strain PAL 5, nitrogen fixing bacterium, major supplier of fixed nitrogen to sugarcane
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Manually annotated by BRENDA team
strain PAL 5, nitrogen fixing bacterium, major supplier of fixed nitrogen to sugarcane
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Manually annotated by BRENDA team
gene GhGS; allotetraploid, strains 7235 and TM-1, gene GhGS, isozymes GS1 and GS2
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
strain ATCC 43504
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain VT301
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Manually annotated by BRENDA team
isozymes GS1a and GS1b
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
isozymes GS1a and GS1b
UniProt
Manually annotated by BRENDA team
genes GSI, GSIII-1, and GSIII-2
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Manually annotated by BRENDA team
genes GSI, GSIII-1, and GSIII-2
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
pv. Tabaci
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Manually annotated by BRENDA team
srain Y-30
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Manually annotated by BRENDA team
srain Y-30
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
3 enzyme forms: GSI, GSII and GSIII
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain E1F1
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Manually annotated by BRENDA team
strain E1F1
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Manually annotated by BRENDA team
8
Uniprot
Manually annotated by BRENDA team
Chinese mainland strain, Anhui isolate
UniProt
Manually annotated by BRENDA team
isozyme GS2
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Manually annotated by BRENDA team
3 enzyme forms: GSI, GSII and GSIII
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Manually annotated by BRENDA team
strain CCMP 1332, isoform GS1 and GS2
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Manually annotated by BRENDA team
strain CCMP 1332, isoform GS1 and GS2
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Manually annotated by BRENDA team
cultivar Micro-Tom
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Manually annotated by BRENDA team
Sorghum sp.
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Manually annotated by BRENDA team
two isoforms
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Manually annotated by BRENDA team
2 enzyme forms GS1 and GS2
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Manually annotated by BRENDA team
four highly homolgous GSI protein sequences GlnA1, GlnA2, GlnA3, and GlnA4
UniProt
Manually annotated by BRENDA team
strain NRRL B-2682
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Manually annotated by BRENDA team
strain NRRL B-2682
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Manually annotated by BRENDA team
two isoforms GSI and GSII encoded by the two genes glnA and glnB
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Manually annotated by BRENDA team
strain DSM 639, hypothermoacidophilic crenarcheon
SwissProt
Manually annotated by BRENDA team
strain 111
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Manually annotated by BRENDA team
strain 111
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
cyanobacterium
SwissProt
Manually annotated by BRENDA team
strain ATCC 95640
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ATP + 2 L-glutamate + 2 NH3
2 ADP + 2 phosphate + D-glutamine + D-isoglutamine
show the reaction diagram
ATP + 3-aminopentanedioate + hydroxylamine
ADP + phosphate + ?
show the reaction diagram
ATP + 3-aminopentanedioate + hydroxylamine
ADP + phosphate + gamma-glutamylhydroxamate
show the reaction diagram
the enzyme is more selective for L-glutamate (alpha-glutamate) than 3-aminopentanedioate (beta-glutamate) as a substrate
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?
ATP + glutamate + NH4+
ADP + phosphate + L-glutamine
show the reaction diagram
ATP + L-Glu + ethylamine
ADP + phosphate + ?
show the reaction diagram
ATP + L-Glu + ethylamine
ADP + phosphate + L-Gln + ?
show the reaction diagram
ATP + L-Glu + hydroxylamine
ADP + phosphate + gamma-glutamyl hydroxamate
show the reaction diagram
ATP + L-Glu + hydroxylamine
ADP + phosphate + L-Gln + ?
show the reaction diagram
ATP + L-Glu + methylamine
ADP + phosphate + ?
show the reaction diagram
ATP + L-Glu + methylamine
ADP + phosphate + L-Gln + ?
show the reaction diagram
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7% of the activity with NH4+, at pH 8.0, activated with 30 mM Mg2+
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?
ATP + L-Glu + NH4+
?
show the reaction diagram
ATP + L-Glu + NH4+
ADP + phosphate + L-Gln
show the reaction diagram
ATP + L-glutamate + hydroxylamine
?
show the reaction diagram
ATP + L-glutamate + hydroxylamine
ADP + phosphate + gamma-L-glutamyl hydroxamate + NH3
show the reaction diagram
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in presence of Mn2+
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?
ATP + L-glutamate + hydroxylamine
ADP + phosphate + gamma-L-glutamylhydroxamate
show the reaction diagram
ATP + L-glutamate + hydroxylamine
ADP + phosphate + L-gamma-glutamylhydroxamate
show the reaction diagram
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photometric determination of GS activity based on formation of an L-gamma-glutamylhydroxamate ferric chloride complex using hydroxylamine instead of ammonia
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?
ATP + L-glutamate + NH3
ADP + phosphate + L-glutamine
show the reaction diagram
ATP + L-glutamate + NH4+
ADP + phosphate + L-glutamine
show the reaction diagram
Gln + hydroxylamine + ADP
gamma-Glutamylhydroxamate + NH4+ + ?
show the reaction diagram
GTP + L-Glu + NH4+
GDP + phosphate + L-Gln
show the reaction diagram
hydroxylamine + L-glutamine + ATP
L-gamma-glutamyl-hydroxamate + ammonia + ADP
show the reaction diagram
hydroxylamine + L-glutamine + ATP
L-gamma-glutamyl-hydroxamate + ammonium + ADP
show the reaction diagram
ITP + L-Glu + NH4+
IDP + phosphate + L-Gln
show the reaction diagram
L-Gln + hydroxylamine + ADP
gamma-glutamylhydroxamate + NH4+ + ?
show the reaction diagram
L-Gln + hydroxylamine + ADP
L-gamma-glutamylhydroxamate + NH4+ + ?
show the reaction diagram
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?
L-glutamine + hydroxylamine + ADP
gamma-glutamylhydroxamate + NH3 + ?
show the reaction diagram
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partial reverse reaction
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?
L-glutamine + hydroxylamine + ADP
gamma-glutamylhydroxamate + NH4+ + ?
show the reaction diagram
TTP + L-Glu + NH4+
TDP + phosphate + L-Gln
show the reaction diagram
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10% of the activity relative to ATP
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UTP + L-Glu + NH4+
UDP + phosphate + L-Gln
show the reaction diagram
additional information
?
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