Cloned (Comment) | Organism |
---|---|
overexpression of native and selenomethionyl beta-PGM in escherichia coli | Lactococcus lactis |
Crystallization (Comment) | Organism |
---|---|
beta-PGM is transferred to 1 mM HEPES, pH 7.5, 10 mM MgCl2 and 0.1 mM dithiothreitol, final beta-PGM concentration at 15 mg/ml, crystals are obtained from either 150 mM ammonium acetate, 100 mM trisodium citrate dihydrate, pH 4.5, 25% polyethylene glycol 4000 or 100 mM ammonium fluoride, 16% polyethylene glycol 3350 by hanging-drop vapor diffusion, crystals of selenium methionyl-substituted beta-PGM diffract to 2.3 A | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
beta-D-glucose 1-phosphate | in the presence of beta-D-glucose 1,6-diphosphate and Mg2+ | Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Lactococcus lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-Glucose 1-phosphate | Lactococcus lactis | - |
beta-D-Glucose 6-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant native and selenomethionyl beta-PGM | Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
native beta-PGM | Lactococcus lactis |
40 | - |
recombinant beta-PGM | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-Glucose 1-phosphate | - |
Lactococcus lactis | beta-D-Glucose 6-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
beta-PGM | - |
Lactococcus lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
beta-D-glucose 1-phosphate | in the presence of beta-D-glucose 1,6-diphosphate and Mg2+ | Lactococcus lactis |