Activating Compound | Comment | Organism | Structure |
---|---|---|---|
guanidinium hydrochloride | 2.5fold activation at 500 mM, inhibition above, less than 5% activity at 2 M guanidinium hydrochloride | Mus musculus | |
guanidinium hydrochloride | activation between 100-1000 mM, 3fold activation at 500 mM guanidinium hydrochloride, inhibition above 500 mM, less than 5% activity at 3 M | Homo sapiens | |
guanidinium hydrochloride | activation up to 750 mM, 1.5fold activition at 500 mM, inhibition above 1 M, complete deactivation at 4 M | Mus musculus | |
Urea | 1.6fold activation at 1M, inhibition above, less than 5% activity at 4 M urea | Mus musculus | |
Urea | activation between 250-2000 mM, 1.7fold activation at 1 M urea, inhibition above 500 mM, less than 5% activity at 4 M | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Mus musculus |
expression of cDNA in Escherichia coli | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | in the presence of 1 M urea | Mus musculus | |
0.0028 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Mus musculus | |
0.0083 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | in the presence of 500 mM guanidinium hydrochloride | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | Homo sapiens | - |
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate | - |
? | |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | Mus musculus | - |
(5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | O09114 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Renatured (Comment) | Organism |
---|---|
complete recovery of activity after denaturation with 2 M guanidinium hydrochloride or 4 M urea | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Homo sapiens | (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate | - |
? | |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Mus musculus | (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipocalin-type prostaglandin d synthase | - |
Mus musculus |
lipocalin-type prostaglandin d synthase | i.e. L-PGDS | Homo sapiens |
lipocalin-type prostaglandin d synthase | i.e. L-PGDS | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Mus musculus | |
0.5 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | in the presence of 1 M urea | Mus musculus | |
0.95 | - |
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | in the presence of 500 mM guanidinium hydrochloride | Mus musculus |