Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose | if D-xylose is the carbon source D-glucose supplementation stimulates formation of xylose isomerase | Glutamicibacter nicotianae | |
sucrose | - |
Glutamicibacter nicotianae | |
xylitol | - |
Escherichia coli | |
xylitol | - |
Glutamicibacter nicotianae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | suppressed enzyme synthesis by increased content from 0.1 to 1.5%, productivity maxima at 0.1%, if D-xylose is the carbon source: D-glucose supplementation suppresses formation of xylose isomerase | Escherichia coli | |
D-glucose | suppressed enzyme synthesis by increased D-glucose content from 0.1 to 1.5%, productivity maxima at 1.0% | Glutamicibacter nicotianae | |
D-glucose | suppressed enzyme synthesis by increased D-glucose content from 0.1 to 1.5%, productivity maxima at 0.1% D-glucose, if D-xylose is the carbon source D-glucose supplementation suppresses formation of xylose isomerase | Pectobacterium atrosepticum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Xylose | Escherichia coli | - |
D-Xylulose | - |
r | |
D-Xylose | Glutamicibacter nicotianae | - |
D-Xylulose | - |
r | |
D-Xylose | Pectobacterium atrosepticum | - |
D-Xylulose | - |
r | |
D-Xylose | Glutamicibacter nicotianae BIM B-5 | - |
D-Xylulose | - |
r | |
D-Xylose | Escherichia coli HB101xylA | - |
D-Xylulose | - |
r | |
D-Xylose | Pectobacterium atrosepticum jn42xylA | - |
D-Xylulose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli HB101xylA | - |
- |
- |
Glutamicibacter nicotianae | - |
- |
- |
Glutamicibacter nicotianae BIM B-5 | - |
- |
- |
Pectobacterium atrosepticum | - |
- |
- |
Pectobacterium atrosepticum jn42xylA | - |
- |
- |
Purification (Comment) | Organism |
---|---|
PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Arthrobacter nicotianae and Erwinia carotovora subsp. atroseptica | Escherichia coli |
PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from Escherichia coli and Erwinia carotovora subsp. atroseptica | Glutamicibacter nicotianae |
PAGE and SDS-PAGE, identical electrophoretic mobility of intact and denatured enzyme compared with xylose isomerase from from Arthrobacter nicotianae and Escherichia coli | Pectobacterium atrosepticum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.22 | - |
enzyme production in the presence of D-glucose | Escherichia coli |
0.46 | - |
crude extract | Pectobacterium atrosepticum |
0.46 | - |
enzyme production in the presence of D-xylose | Escherichia coli |
0.47 | - |
enzyme production in the presence of D-xylose | Glutamicibacter nicotianae |
0.71 | - |
enzyme production in the presence of D-xylose | Pectobacterium atrosepticum |
0.73 | - |
enzyme production in the presence of sucrose | Glutamicibacter nicotianae |
0.81 | - |
enzyme production in the presence of D-glucose | Pectobacterium atrosepticum |
1.39 | - |
enzyme production in the presence of xylitol | Escherichia coli |
1.63 | - |
crude extract | Glutamicibacter nicotianae |
1.69 | - |
crude extract | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose | lower efficiency | Escherichia coli | ? | - |
? | |
D-glucose | lower efficiency | Glutamicibacter nicotianae | ? | - |
? | |
D-glucose | lower efficiency | Pectobacterium atrosepticum | ? | - |
? | |
D-glucose | lower efficiency | Glutamicibacter nicotianae BIM B-5 | ? | - |
? | |
D-glucose | lower efficiency | Escherichia coli HB101xylA | ? | - |
? | |
D-glucose | lower efficiency | Pectobacterium atrosepticum jn42xylA | ? | - |
? | |
D-Ribose | weak effects | Escherichia coli | ? | - |
? | |
D-Ribose | weak effects | Glutamicibacter nicotianae | ? | - |
? | |
D-Ribose | weak effects | Pectobacterium atrosepticum | ? | - |
? | |
D-Ribose | weak effects | Glutamicibacter nicotianae BIM B-5 | ? | - |
? | |
D-Ribose | weak effects | Escherichia coli HB101xylA | ? | - |
? | |
D-Ribose | weak effects | Pectobacterium atrosepticum jn42xylA | ? | - |
? | |
D-Xylose | - |
Escherichia coli | D-Xylulose | - |
r | |
D-Xylose | - |
Glutamicibacter nicotianae | D-Xylulose | - |
r | |
D-Xylose | - |
Pectobacterium atrosepticum | D-Xylulose | - |
r | |
D-Xylose | - |
Glutamicibacter nicotianae BIM B-5 | D-Xylulose | - |
r | |
D-Xylose | - |
Escherichia coli HB101xylA | D-Xylulose | - |
r | |
D-Xylose | - |
Pectobacterium atrosepticum jn42xylA | D-Xylulose | - |
r | |
L-Arabinose | weak effects | Escherichia coli | ? | - |
? | |
L-Arabinose | weak effects | Glutamicibacter nicotianae | ? | - |
? | |
L-Arabinose | weak effects | Pectobacterium atrosepticum | ? | - |
? | |
L-Arabinose | weak effects | Glutamicibacter nicotianae BIM B-5 | ? | - |
? | |
L-Arabinose | weak effects | Escherichia coli HB101xylA | ? | - |
? | |
L-Arabinose | weak effects | Pectobacterium atrosepticum jn42xylA | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-xylose: ketol-isomerase | - |
Escherichia coli |
D-xylose: ketol-isomerase | - |
Glutamicibacter nicotianae |
D-xylose: ketol-isomerase | - |
Pectobacterium atrosepticum |
xylose isomerase | - |
Escherichia coli |
xylose isomerase | - |
Glutamicibacter nicotianae |
xylose isomerase | - |
Pectobacterium atrosepticum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Escherichia coli |
70 | - |
- |
Glutamicibacter nicotianae |
75 | - |
- |
Pectobacterium atrosepticum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
pH 7.5, 2 h | Escherichia coli |
70 | - |
pH 7.5, 2 h | Glutamicibacter nicotianae |
70 | 80 | pH 7.8, 2 h, after following 2 h incubation at 80°C 37.6% loss of activity, 70-75% loss of activity at 80°C | Pectobacterium atrosepticum |
80 | - |
2 h leads to 30.1% loss of activity | Escherichia coli |
80 | - |
2 h leads to 30.5% loss of activity | Glutamicibacter nicotianae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |
7.5 | - |
- |
Glutamicibacter nicotianae |
8 | - |
- |
Pectobacterium atrosepticum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 70°C for 1 h, 70-75% loss of activity at 80°C | Escherichia coli |
7 | 8 | 70°C for 1 h, 70-75% loss of activity at 80°C | Glutamicibacter nicotianae |
7 | 8 | 70°C for 1 h, 70-75% loss of activity at 80°C | Pectobacterium atrosepticum |