Information on EC 5.3.1.5 - Xylose isomerase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
5.3.1.5
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RECOMMENDED NAME
GeneOntology No.
Xylose isomerase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-xylopyranose = D-xylulose
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isomerization
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
d-xylose degradation
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Fructose and mannose metabolism
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Metabolic pathways
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Pentose and glucuronate interconversions
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xylose degradation I
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SYSTEMATIC NAME
IUBMB Comments
D-xylose aldose-ketose-isomerase
Contains two divalent metal ions, preferably magnesium, located at different metal-binding sites within the active site. The enzyme catalyses the interconversion of aldose and ketose sugars with broad substrate specificity. The enzyme binds the closed form of its sugar substrate (in the case of glucose, only the alpha anomer) and catalyses ring opening to generate a form of open-chain conformation that is coordinated to one of the metal sites. Isomerization proceeds via a hydride-shift mechanism.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-82-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Actinomyces olivocinereus
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
Aerobacter levanicum
-
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Arthrobacter nicotianae
Arthrobacter nicotianae BIM B-5
strain BIM B-5
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-
Manually annotated by BRENDA team
Arthrobacter nicotianae BIM V-5
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-
Manually annotated by BRENDA team
Arthrobacter ureafaciens
induced by presence of xylose and xylite. The synthesis of enzyme is controlled by catabolite repression occuring at the transcriptional level and mediated by cyclic 3',5'-AMP
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-
Manually annotated by BRENDA team
strain HN-68
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Bacillus sp. No. KX-6
No. KX-6
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-
Manually annotated by BRENDA team
TX-3
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-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
Brevibacterium pentosoaminoacidicum
-
-
-
Manually annotated by BRENDA team
Burkholderia sacchari
strain IPT101, gene xylA
Uniprot
Manually annotated by BRENDA team
Burkholderia sacchari IPT101
strain IPT101, gene xylA
Uniprot
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
Escherichia coli DH5-alpha
strain DH5alpha
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-
Manually annotated by BRENDA team
Escherichia coli HB101xylA
strain HB101xylA
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-
Manually annotated by BRENDA team
barley
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Manually annotated by BRENDA team
Lactobacillus gayonii
-
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-
Manually annotated by BRENDA team
Lactobacillus lycopersici
-
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-
Manually annotated by BRENDA team
Lactobacillus mannitopoeus
-
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
strain 210 (Xyl-); strain IO-1 (Xyl+)
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-
Manually annotated by BRENDA team
Lactococcus lactis 210 (Xyl-)
strain 210 (Xyl-)
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-
Manually annotated by BRENDA team
Lactococcus lactis IO-1 (Xyl+)
strain IO-1 (Xyl+)
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Corynebacterium glutamicum
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-
Manually annotated by BRENDA team
no activity in Hansenula polymorpha
strain CBS4732s leu2-2
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-
Manually annotated by BRENDA team
no activity in Hansenula polymorpha CBS4732s
strain CBS4732s leu2-2
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-
Manually annotated by BRENDA team
no activity in Saccharomyces cerevisiae
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Opuntia vulgaris
-
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Manually annotated by BRENDA team
Orpinomyces sp.
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UniProt
Manually annotated by BRENDA team
Paracolobacterium aerogenoides
-
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Manually annotated by BRENDA team
strain jn42xylA
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-
Manually annotated by BRENDA team
strain jn42xylA
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
Sarcina sp.
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Manually annotated by BRENDA team
gene XYL1
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Manually annotated by BRENDA team
Streptococcus venuceus
-
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Manually annotated by BRENDA team
NRRL 5778
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
recombinant enzyme from Hansenula polymorpha transformants
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-
Manually annotated by BRENDA team
strain J-59
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Manually annotated by BRENDA team
strain J-59
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Manually annotated by BRENDA team
Streptomyces diastaticus No. 7
strain M1033
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Manually annotated by BRENDA team
Streptomyces diastaticus No. 7 M1033
strain M1033
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Streptomyces nivens
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Streptomyces sp. NCIM 2730
NCIM 2730
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Manually annotated by BRENDA team
Thermoanaerobacter ethanolicus JW200 (ATCC 31550)
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UniProt
Manually annotated by BRENDA team
B6A-RI
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Manually annotated by BRENDA team
strain JW/SL-YS 489
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Manually annotated by BRENDA team
strain JW/SL-YS 489
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Manually annotated by BRENDA team
Thermopolyspora sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain HB8
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Manually annotated by BRENDA team
strain HB8
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-glucose
alpha-D-fructose
show the reaction diagram
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-
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r
D-Arabinose
?
show the reaction diagram
D-Fructose
?
show the reaction diagram
D-fructose
D-glucose
show the reaction diagram
D-fructose
D-mannose
show the reaction diagram
-
-
-
-
r
D-glucose
?
show the reaction diagram
D-Glucose
D-Fructose
show the reaction diagram
D-glyceraldehyde
dihydroxyacetone
show the reaction diagram
-
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-
-
?
D-Lyxose
?
show the reaction diagram
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-
-
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D-Lyxose
D-Xylulose
show the reaction diagram
-
-
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r
D-Mannose
D-Fructose
show the reaction diagram
-
-
-
-
r
D-Ribose
?
show the reaction diagram
D-Ribose
D-Ribulose
show the reaction diagram
-
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-
r
D-ribose
L-arabinose
show the reaction diagram
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-
r
D-Xylose
?
show the reaction diagram
D-Xylose
D-Xylulose
show the reaction diagram
L-Arabinose
?
show the reaction diagram
L-arabinose
D-ribose
show the reaction diagram
-
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-
r
L-arabinose
D-ribulose
show the reaction diagram
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r
L-Arabinose
L-Ribulose
show the reaction diagram
L-Rhamnose
?
show the reaction diagram
L-ribose
?
show the reaction diagram
-
no increased reaction rate observed for mutant F26W
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?
L-ribose
L-ribulose
show the reaction diagram
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?
L-ribulose
L-ribose
show the reaction diagram
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r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-Glucose
D-Fructose
show the reaction diagram
D-Xylose
?
show the reaction diagram
D-Xylose
D-Xylulose
show the reaction diagram
L-Arabinose
L-Ribulose
show the reaction diagram
-
-
-
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-O-Methyl-D-xylose
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-
5-Thio-alpha-D-glucopyranose
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competitive
8-hydroxyquinoline
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above 5 mM
arsenate
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-
Co2+
-
about 58% residual activity at 1 mM
D-glucose
D-glyceraldehyde
-
competitive inhibition of the isomerization of D-xylose
D-Lyxose
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-
D-mannitol
-
competitive
D-ribose
-
inhibits D-glucose isomerization
D-sorbitol
D-Threonohydroxamic acid
D-xylitol
D-xylose
Dideoxyimino-D-glucitol
-
-
L-arabitol
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Mg2+
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about 35% residual activity at 1 mM
PCMB
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above 5 mM
ribose
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Tris(hydroxymethyl)aminomethane
xylitol
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glucose
Arthrobacter nicotianae
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if D-xylose is the carbon source D-glucose supplementation stimulates formation of xylose isomerase
Sucrose
Arthrobacter nicotianae
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xylitol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
151
D-arabinose
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-
50 - 800
D-fructose
0.249 - 5800
D-glucose
3
D-glyceraldehyde
-
in D2O, 24 mM imidazole buffer, 10 mM MgCl2, at pH 7.5 and 25C
215
D-Lyxose
-
mutant enzyme D256R, at pH 7.0 and 85C
185 - 1005
D-mannose
77 - 670
D-ribose
0.076 - 605
D-xylose
8 - 100
D-xylulose
138 - 1500
L-arabinose
312
L-rhamnose
-
-
438 - 1312
L-ribose
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3
D-arabinose
Thermus aquaticus
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-
1.67 - 20.5
D-fructose
0.03 - 172.6
D-glucose
0.0001
D-glyceraldehyde
Streptomyces rubiginosus
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in D2O, 24 mM imidazole buffer, 10 mM MgCl2, at pH 7.5 and 25C
0.0617 - 38
D-Lyxose
2 - 18
D-mannose
0.515
D-ribose
Arthrobacter sp.
-
30C
0.007 - 258
D-xylose
7.7
D-xylulose
Thermus aquaticus
-
-
0.1 - 13
L-arabinose
0.0226 - 0.0252
L-ribose
additional information
additional information
Actinoplanes missouriensis
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turnover numbers for wild-type enzyme and mutant enzymes E186D and E186Q, activated by Mg2+, Mn2+ or Co2+
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