Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | silver inhibits competitively | Oncorhynchus mykiss | |
Cd2+ | cadmium inhibits competitively | Oncorhynchus mykiss | |
Co2+ | cobalt inhibits competitively | Oncorhynchus mykiss | |
Cu2+ | copper inhibits noncompetitively | Oncorhynchus mykiss | |
Zn2+ | zinc inhibits uncompetitively | Oncorhynchus mykiss |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27300 | - |
native enzyme, estimated by gel filtration | Oncorhynchus mykiss |
29000 | - |
subunit, SDS-PAGE | Oncorhynchus mykiss |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | Oncorhynchus mykiss | - |
H2CO3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oncorhynchus mykiss | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography | Oncorhynchus mykiss |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Oncorhynchus mykiss | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme from lysate shows a specific activity of 1.77 EU/mg, the 1280fold purified enzyme shows a specific activity of 2275 EU/mg | Oncorhynchus mykiss |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + H2O | - |
Oncorhynchus mykiss | H2CO3 | - |
r |
Synonyms | Comment | Organism |
---|---|---|
carbonate hydrolyase | - |
Oncorhynchus mykiss |
carbonic anhydrase | - |
Oncorhynchus mykiss |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
in 1 M Tris-SO4 at 4°C | Oncorhynchus mykiss |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
stable at pH 9.0 in 1 M Tris-SO4 at 4°C | Oncorhynchus mykiss |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
Co2+ | in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | |
2.15 | - |
Zn2+ | in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | |
27.68 | - |
Cu2+ | in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | |
94.18 | - |
Cd2+ | in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | |
193.9 | - |
Ag+ | in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.05 | - |
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | Co2+ | |
0.31 | - |
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | Zn2+ | |
30 | - |
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | Cu2+ | |
82.5 | - |
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | Cd2+ | |
159 | - |
in 0.05 M Tris-SO4 buffer, at pH 9.0 and 25°C | Oncorhynchus mykiss | Ag+ |