Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dihydroxyacetone phosphate | activates | Chlamydomonas reinhardtii | |
glutamine | activates | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Chlamydomonas reinhardtii | |
Asp | - |
Chlamydomonas reinhardtii | |
Glu | - |
Chlamydomonas reinhardtii | |
malate | - |
Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Chlamydomonas reinhardtii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
sequencing of the N-terminus, peptide mapping and immunological data suggest that the catalytic subunit of the enzyme is not related to the prokaryotic enzyme and is only distantly related to higher plant C4 and C3 enzymes | Chlamydomonas reinhardtii |
100000 | - |
4 * 100000, isoenzyme PEPC1, SDS-PAGE | Chlamydomonas reinhardtii |
400000 | - |
low-molecular-mass isoenzyme PEPC1, gel filtration | Chlamydomonas reinhardtii |
650000 | - |
high-molecular-weight isoenzyme PEPC2, gel filtration | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Phosphoenolpyruvate + CO2 | Chlamydomonas reinhardtii | key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae | ? | - |
? | |
Phosphoenolpyruvate + CO2 | Chlamydomonas reinhardtii CW-15 cc1883 | key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | - |
- |
- |
Chlamydomonas reinhardtii CW-15 cc1883 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
a high-molecular-mass isoform, PEPC2, and a low-molecular-mass isoform, PEPC1 | Chlamydomonas reinhardtii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.11 | - |
- |
Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Chlamydomonas reinhardtii | phosphate + oxaloacetate | - |
? | |
phosphoenolpyruvate + CO2 | - |
Chlamydomonas reinhardtii CW-15 cc1883 | phosphate + oxaloacetate | - |
? | |
Phosphoenolpyruvate + CO2 | key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae | Chlamydomonas reinhardtii | ? | - |
? | |
Phosphoenolpyruvate + CO2 | key enzyme in the supply of carbon skeleton for the assimilation of nitrogen by green algae | Chlamydomonas reinhardtii CW-15 cc1883 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | isoenzyme PEPC2 is a complex between the PEPC catalytic subunit of MW 100000 Da and other immunologically unrelated polypeptides of 50000-700000 Da | Chlamydomonas reinhardtii |
tetramer | 4 * 100000, isoenzyme PEPC1, SDS-PAGE | Chlamydomonas reinhardtii |