Crystallization (Comment) | Organism |
---|---|
mutant S175A in complex with chloroacetate, 2-L-chlorobutyrate, 2-L-chloro-3-methylbutyrate, 2-L-chloro-4-methylvalerate, and L-2-chloropropionamide, all substrates bound have D-configuration, except for chloroacetate | Pseudomonas sp. |
Protein Variants | Comment | Organism |
---|---|---|
D180A | site-directed mutagenesis of a residue that strongly interacts with the substrate | Pseudomonas sp. |
K151A | site-directed mutagenesis of a residue that strongly interacts with the substrate | Pseudomonas sp. |
S175A | site-directed mutagenesis of a residue that strongly interacts with the substrate | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-chloropropionic acid + H2O | Pseudomonas sp. | - |
(R)-2-hydroxypropionic acid + chloride | - |
? | |
(S)-2-chloropropionic acid + H2O | Pseudomonas sp. 113 | - |
(R)-2-hydroxypropionic acid + chloride | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide | reaction mechanism, overview | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-chloropropionic acid + H2O | - |
Pseudomonas sp. | (R)-2-hydroxypropionic acid + chloride | - |
? | |
(S)-2-chloropropionic acid + H2O | the substrate interacts strongly with Asp10, Arg41, Lys151, and Asp180 | Pseudomonas sp. | (R)-2-hydroxypropionic acid + chloride | - |
? | |
(S)-2-chloropropionic acid + H2O | - |
Pseudomonas sp. 113 | (R)-2-hydroxypropionic acid + chloride | - |
? | |
additional information | the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview | Pseudomonas sp. | ? | - |
? | |
additional information | the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview | Pseudomonas sp. 113 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | docking and structure analysis of enzyme mutants K151A and D180A in complex with substrate (S)-2-chloropropionic acid, molecular dynamics, overview | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
L-2-haloacid dehalogenase | - |
Pseudomonas sp. |
L-DEX YL | - |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | single turnover | Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the HAD superfamily | Pseudomonas sp. |