Information on EC 3.8.1.2 - (S)-2-haloacid dehalogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
3.8.1.2
-
RECOMMENDED NAME
GeneOntology No.
(S)-2-haloacid dehalogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
acts on acids of short chain lengths, C2 to C4 with inversion at C2
-
-
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product; mechanism
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
the stereochemistry of the product obtained from racemic 2-brompropionic acid by the action of celite-immobilized disrupted cells in dimethylsulfoxid differs from that of the enantiomer obtained in an aqueous buffer
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
functional residue: Asp 10
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
reaction mechanism, overview
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
the stereochemistry of the product obtained from racemic 2-brompropionic acid by the action of celite-immobilized disrupted cells in dimethylsulfoxid differs from that of the enantiomer obtained in an aqueous buffer
Pseudomonas putida No. 109
-
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product
Pseudomonas putida YL
-
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product; Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product; mechanism; reaction mechanism, overview
-
-
(S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide
show the reaction diagram
Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product; Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product; Asp10 of the enzyme functions as a catalytic nucleophile: the residue attacks the alpha-carbon of the substrate to form an ester intermediate, which is subsequently hydrolysed to release the product; functional residue: Asp 10; functional residue: Asp 10; mechanism; reaction mechanism, overview
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
C-halide hydrolysis
-
-
-
-
C-halide hydrolysis
-
-
C-halide hydrolysis
Pseudomonas putida AJ1
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
2-chloroacrylate degradation I
-
Chloroalkane and chloroalkene degradation
-
Chlorocyclohexane and chlorobenzene degradation
-
Metabolic pathways
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
(S)-2-haloacid halidohydrolase
Acts on acids of short chain lengths, C2 to C4, with inversion of configuration at C-2. [See also EC 3.8.1.9 (R)-2-haloacid dehalogenase, EC 3.8.1.10 2-haloacid dehalogenase (configuration-inverting) and EC 3.8.1.11 2-haloacid dehalogenase (configuration-retaining)]
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2-halo acid dehalogeanse
-
-
-
-
2-haloacid dehalogenase[ambiguous]
-
-
-
-
2-haloacid halidohydrolase[ambiguous]
-
-
-
-
2-haloalkanoic acid dehalogenase
-
-
-
-
2-haloalkanoid acid halidohydrolase
-
-
-
-
2-halocarboxylic acid dehalogenase II
-
-
-
-
dehalogenase IVa
-
-
-
-
DL-2-haloacid dehalogenase [ambiguous]
-
-
-
-
L-2-haloacid dehalogenase
-
-
-
-
L-2-haloacid dehalogenase
-
-
L-2-haloacid dehalogenase
-
-
-
L-2-haloacid dehalogenase
-
-
L-2-haloacid dehalogenase
Pyrococcus horikoshii OT-3
-
-
-
L-2-MCPA dehalogenase
-
-
L-2_HAD
Q96XE7
-
L-2_HAD
Sulfolobus tokodaii 7
Q96XE7
-
-
L-DEX
-
-
-
-
L-DEX YL
-
-
-
-
L-DEX YL
-
-
L-DEXs
-
-
-
-
L-haloacid dehalogenase
Q96XE7
-
L-haloacid dehalogenase
Sulfolobus tokodaii 7
Q96XE7
-
-
PH1421
Pyrococcus horikoshii OT-3
-
-
-
S-2-haloacid dehalogenase
-
-
STK_25700
Q96XE7
locus name
STK_25700
Sulfolobus tokodaii 7
Q96XE7
locus name
-
CAS REGISTRY NUMBER
COMMENTARY
37289-39-7
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain RS5, cryptic gene
-
-
Manually annotated by BRENDA team
Agrobacterium tumefaciens RS5
strain RS5, cryptic gene
-
-
Manually annotated by BRENDA team
Azotobacter sp.
strain RC26
-
-
Manually annotated by BRENDA team
Azotobacter sp. RC26
strain RC26
-
-
Manually annotated by BRENDA team
strain MBA4
-
-
Manually annotated by BRENDA team
strain MBA4, i.e. Pseudomonas cepacia MBA4
-
-
Manually annotated by BRENDA team
Burkholderia cepacia MBA4
MBA4
-
-
Manually annotated by BRENDA team
Burkholderia cepacia MBA4
strain MBA4
-
-
Manually annotated by BRENDA team
Burkholderia cepacia MBA4
strain MBA4, i.e. Pseudomonas cepacia MBA4
-
-
Manually annotated by BRENDA team
from the intertidal coast of the Chinese Yellow Sea near Dalian City
-
-
Manually annotated by BRENDA team
strain B
-
-
Manually annotated by BRENDA team
strain B
-
-
Manually annotated by BRENDA team
AJ1; No. 109
-
-
Manually annotated by BRENDA team
Pseudomonas putida AJ1
AJ1
SwissProt
Manually annotated by BRENDA team
Pseudomonas putida AJ1
AJ1
-
-
Manually annotated by BRENDA team
Pseudomonas putida No. 109
No. 109
-
-
Manually annotated by BRENDA team
Pseudomonas putida YL
YL
-
-
Manually annotated by BRENDA team
strain 113
-
-
Manually annotated by BRENDA team
strain 113; strain YL
-
-
Manually annotated by BRENDA team
strain CBS3 stain CBS3 <31,41>
-
-
Manually annotated by BRENDA team
strain CBS3 stain CBS3 <31,41>
-
-
Manually annotated by BRENDA team
strain OT3
-
-
Manually annotated by BRENDA team
Pyrococcus horikoshii OT-3
strain OT3
-
-
Manually annotated by BRENDA team
201 amino acids long hypothetical 2-haloalkanoic acid dehalogenase; strain 7
UniProt
Manually annotated by BRENDA team
Sulfolobus tokodaii 7
-
UniProt
Manually annotated by BRENDA team
Sulfolobus tokodaii 7
201 amino acids long hypothetical 2-haloalkanoic acid dehalogenase; strain 7
UniProt
Manually annotated by BRENDA team
Xanthobacter autotrophicus GJ10
GJ10
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
evolution
-
the enzyme belongs to the HAD superfamily
evolution
-
the enzyme belongs to the Group II family
evolution
-
the enzyme belongs to the HAD superfamily
-
physiological function
-
DEH99 is a (S)-2-haloacid dehalogenase, which can degrade (S)-2-chloropropionic acid, (S)-2-bromopropionic acid, and iodoacetic acid
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R,S)-2-bromopropionic acid + H2O
(S)-lactic acid + (S)-2-bromopropionic acid + HCl
show the reaction diagram
-
-
-
-
?
(R,S)-2-chloropropionic acid + H2O
(S)-lactic acid + (S)-2-chloropropionic acid + HCl
show the reaction diagram
-
-
-
-
-
(R,S)-2-chloropropionic acid + H2O
(S)-lactic acid + (S)-2-chloropropionic acid + HCl
show the reaction diagram
-
-
-
-
?
(R,S)-2-chloropropionic acid + H2O
(S)-lactic acid + (S)-2-chloropropionic acid + HCl
show the reaction diagram
Pseudomonas putida AJ1
-
-
-
-
-
(S)-2-bromopropionic acid + H2O
(R)-2-hydroxypropionic acid + bromide
show the reaction diagram
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
high activity
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
enantioselective
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
the substrate interacts strongly with Asp10, Arg41, Lys151, and Asp180
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
?
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Q52087
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Q51645
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Azotobacter sp.
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Q60099
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
enzyme of 2-chloropropionate grown cells
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
substrate specificity towards chlorinated and bromated substrates is limited to short chain monosubstituated 2-halocarboxylic acids, fluoro compounds are not converted
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
reaction by inversion of the configuration in a mixture of dimethylsulfoxide and buffer, but in presence of stoichiometric amounts of water proceeds without inversion of the configuration contrary to the behavior in an aqueous environment
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
substrate specificity towards chlorinated and bromated substrates is limited to short chain monosubstituated 2-halocarboxylic acids, fluoro compounds are not converted
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Pseudomonas putida YL
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Pseudomonas putida AJ1
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Pseudomonas putida AJ1
Q52087
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Burkholderia cepacia MBA4
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Azotobacter sp. RC26
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Xanthobacter autotrophicus GJ10
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Pseudomonas putida No. 109
-
-
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Pseudomonas putida No. 109
-
reaction by inversion of the configuration in a mixture of dimethylsulfoxide and buffer, but in presence of stoichiometric amounts of water proceeds without inversion of the configuration contrary to the behavior in an aqueous environment
-
-
?
(S)-2-haloacid + H2O
(R)-2-hydroxyacid + halide
show the reaction diagram
Pseudomonas putida No. 109, Moraxella sp. B
-
-
-
-
?
1,2-dichloroethane + H2O
?
show the reaction diagram
Xanthobacter autotrophicus, Xanthobacter autotrophicus GJ10
-
-
-
-
?
2,2-dichloropropionate + H2O
propionate + HCl
show the reaction diagram
-
-
-
-
?
2,2-dichloropropionic acid + H2O
propionate + HCl
show the reaction diagram
-
-
-
-
?
2,2-dichloropropionic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2,3-dibromopropionate + H2O
?
show the reaction diagram
Q52087
-
-
-
?
2,3-dibromopropionate + H2O
?
show the reaction diagram
-
-
-
-
?
2,3-dibromopropionate + H2O
?
show the reaction diagram
Pseudomonas putida AJ1
Q52087
-
-
-
?
2,3-dibromopropionate + H2O
?
show the reaction diagram
Pseudomonas putida No. 109
-
-
-
-
?
2,3-dichloropropionate + H2O
?
show the reaction diagram
Pseudomonas putida, Pseudomonas putida AJ1
Q52087
-
-
-
?
2,3-dichloropropionic acid + H2O
3-chlorlactic acid + HCl
show the reaction diagram
Pseudomonas putida, Pseudomonas putida No. 109
-
-
-
?
2-bromohexadecanoate + H2O
D-2-hydroxyhexadecanoate + HBr
show the reaction diagram
-
-
-
-
?
2-bromopropionic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2-bromopropionic acid + H2O
?
show the reaction diagram
Pseudomonas putida, Pseudomonas putida No. 109
-
-
-
-
?
2-chloro-3-hydroxypropionate + H2O
?
show the reaction diagram
Pseudomonas putida, Pseudomonas putida No. 109
-
-
-
-
?
2-chloro-n-butyrate + H2O
?
show the reaction diagram
Pseudomonas putida, Pseudomonas putida No. 109
-
-
-
-
?
2-chloroacrylate + H2O
2-hydroxyacrylate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Q51645
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Azotobacter sp.
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Burkholderia cepacia MBA4
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Agrobacterium tumefaciens RS5
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Azotobacter sp. RC26
-
-
-
-
?
2-chloropropionic acid + H2O
?
show the reaction diagram
Pseudomonas putida, Pseudomonas putida No. 109
-
-
-
-
?
2-monobromobutyrate + H2O
?
show the reaction diagram
Q52087
-
-
-
?
2-monochlorobutyrate + H2O
?
show the reaction diagram
Pseudomonas putida, Pseudomonas putida AJ1
Q52087
-
-
-
?
alpha-bromophenylacetic acid + H2O
?
show the reaction diagram
-
-
-
-
?
dichloroacetate + H2O
?
show the reaction diagram
-
-
-
-
?
dichloroacetate + H2O
?
show the reaction diagram
-
-
-
-
?
dichloroacetic acid + H2O
?
show the reaction diagram
-
-
-
-
?
dichloroacetic acid + H2O
?
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens RS5
-
-
-
-
?
DL-2-bromo-n-butyrate + H2O
?
show the reaction diagram
-
-
-
-
?
DL-2-bromo-n-butyrate + H2O
?
show the reaction diagram
-
-
-
-
?
DL-2-bromo-n-butyrate + H2O
?
show the reaction diagram
-
-
-
-
?
DL-2-bromopropionic acid + H2O
?
show the reaction diagram
-
-
-
-
?
DL-2-chloropropionate + H2O
?
show the reaction diagram
-
-
-
-
?
DL-2-haloalkanoic acid + H2O
L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
DL-2-haloalkanoic acid + H2O
L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
fatty acids + H2O
?
show the reaction diagram
-
monohaloacids, short chain length C2 to C4
-
-
?
fatty acids + H2O
?
show the reaction diagram
-
monohaloacids, short chain length C2 to C4
-
-
?
iodoacetic acid + H2O
acetic acid + iodide + H+
show the reaction diagram
-
-, best substrate
-
-
?
L-2-bromobutanoate + H2O
2-hydroxybutanoate + HBr
show the reaction diagram
Sulfolobus tokodaii, Sulfolobus tokodaii 7
Q96XE7
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-bromohexanoate + H2O
2-hydroxyhexanoate + HBr
show the reaction diagram
Sulfolobus tokodaii, Sulfolobus tokodaii 7
Q96XE7
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-bromopropionate + H2O
lactate + HBr
show the reaction diagram
Sulfolobus tokodaii, Sulfolobus tokodaii 7
Q96XE7
the enzyme catalyzes the stereospecific removal of bromide from the L-conformer but not the D-conformer
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
-
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
-
-
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
Q52087
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
Q52087
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
-
-
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
Pseudomonas putida AJ1
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
Pseudomonas putida AJ1
Q52087
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
Xanthobacter autotrophicus GJ10
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
Xanthobacter autotrophicus GJ10
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
Pseudomonas putida No. 109
-
-
-
?
L-2-chloropropionate + H2O
D-lactate + HCl
show the reaction diagram
Pseudomonas putida No. 109
-
-
-
?
L-2-chloropropionate + H2O
?
show the reaction diagram
-
-
-
-
?
L-2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Sulfolobus tokodaii, Sulfolobus tokodaii 7
Q96XE7
the enzyme catalyzes the stereospecific removal of chloride from the L-conformer but not the D-conformer
-
-
?
L-2-chloropropionic acid + H2O
D-2-chloropropionic acid + H2O
show the reaction diagram
-
-
-
-
?
L-2-chloropropionic acid + H2O
D-2-chloropropionic acid + H2O
show the reaction diagram
-
-
-
-
?
L-2-chloropropionic acid + H2O
D-2-chloropropionic acid + H2O
show the reaction diagram
-
-
-
-
?
L-2-chloropropionic acid + H2O
D-2-chloropropionic acid + H2O
show the reaction diagram
-
-
-
-
?
L-2-chloropropionic acid + H2O
D-2-hydroxypropionate + HCl
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Q52087
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Q51645
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Azotobacter sp.
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Q60099
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Pseudomonas putida YL
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Burkholderia cepacia MBA4
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Azotobacter sp. RC26
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Xanthobacter autotrophicus GJ10
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Pseudomonas putida No. 109
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
Q51645
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
Agrobacterium tumefaciens RS5
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-, ?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Q52087
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Q51645
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-, ?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Azotobacter sp.
-
-
-
-
-
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-, ?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-, ?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Burkholderia cepacia MBA4
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Agrobacterium tumefaciens RS5
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-, ?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-, ?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Azotobacter sp. RC26
-
-
-
-
-
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Xanthobacter autotrophicus GJ10
-
-
-
-
?
monoiodoacetate + H2O
glycolate + HI
show the reaction diagram
-
-
-
-
?
monoiodoacetate + H2O
glycolate + HI
show the reaction diagram
-
-
-
-
?
monoiodoacetate + H2O
glycolate + HI
show the reaction diagram
Agrobacterium tumefaciens, Agrobacterium tumefaciens RS5
-
-
-
-
-
additional information
?
-
-
PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+
-
-
-
additional information
?
-
-
no activity with 2-fluoropropionic acid
-
-
-
additional information
?
-
-
the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview
-
-
-
additional information
?
-
Q96XE7
the enzyme is not active toward 1,4-dibromobutane
-
-
-
additional information
?
-
-
the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview
-
-
-
additional information
?
-
Sulfolobus tokodaii 7
Q96XE7
the enzyme is not active toward 1,4-dibromobutane
-
-
-
additional information
?
-
-
the enzyme reaction mechanism proceeds via an ester intermediate and a nucleophilic aspartate attacking the C-atom of the substrate to displace the halo-atom, mass spectrometral analysis of the structural changes during catalysis, overview
-
-
-
additional information
?
-
Pyrococcus horikoshii OT-3
-
PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-2-bromopropionic acid + H2O
(R)-2-hydroxypropionic acid + bromide
show the reaction diagram
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
-
-
-
?
(S)-2-chloropropionic acid + H2O
(R)-2-hydroxypropionic acid + chloride
show the reaction diagram
-
-
-
-
?
1,2-dichloroethane + H2O
?
show the reaction diagram
Xanthobacter autotrophicus, Xanthobacter autotrophicus GJ10
-
-
-
-
?
2,2-dichloropropionic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Q51645
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Azotobacter sp.
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Agrobacterium tumefaciens RS5
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
-
-
-
-
?
2-chloropropionate + H2O
lactate + HCl
show the reaction diagram
Azotobacter sp. RC26
-
-
-
-
?
DL-2-haloalkanoic acid + H2O
L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
DL-2-haloalkanoic acid + H2O
L-2-hydroxyalkanoic acid + D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
iodoacetic acid + H2O
acetic acid + iodide + H+
show the reaction diagram
-
-
-
-
?
L-2-chloropropionic acid + H2O
D-2-chloropropionic acid + H2O
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Q52087
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Q51645
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Azotobacter sp.
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Q60099
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Pseudomonas putida YL
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Burkholderia cepacia MBA4
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Azotobacter sp. RC26
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Xanthobacter autotrophicus GJ10
-
-
-
-
?
L-2-haloalkanoic acid + H2O
D-2-hydroxyalkanoic acid + halide
show the reaction diagram
Pseudomonas putida No. 109
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
-
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
Q51645
-
-
-
?
monobromoacetate + H2O
glycolate + HBr
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Q51645
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
?
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
-
-
-
-
-
monochloroacetate + H2O
glycolate + HCl
show the reaction diagram
Xanthobacter autotrophicus GJ10
-
-
-
-
?
monoiodoacetate + H2O
glycolate + HI
show the reaction diagram
-
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Mg2+
-
dependent on
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
-
Cu2+
Azotobacter sp.
-
-
CuSO4
Q51645
41% inhibition
FeSO4
Azotobacter sp.
-
above 10 mM
FeSO4
-
above 10 mM
HgCl2
-
high concentrations
iodoacetamide
-
-
mercury(II)-acetate
-
-
N-ethylmaleimide
-
-
p-Chloromercuriphenylsulfonic acid
-
-
p-mercuric chlorobenzoate
-
-
-
p-mercuric chlorobenzoate
-
-
-
p-mercuric chlorobenzoate
Q51645
-
-
thiol reagents
-
not
thiol reagents
-
only when recombinant in E.coli
Woodward reagent K
-
-
MnSO4
-
above 10 mM
additional information
Q96XE7, -
no inhibition with up to 100 mM of D/L-lactate or pyruvate
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.2
-
(S)-2-chloropropionic acid
-
pH 9.0, 50C, soluble enzyme
1.6
-
(S)-2-chloropropionic acid
-
pH 9.0, 22C, immobilized enzyme
2.7
-
(S)-2-chloropropionic acid
-
pH 9.0, 37C, immobilized enzyme
4.9
-
(S)-2-chloropropionic acid
-
pH 9.0, 50C, immobilized enzyme
7.5
-
2-monochloropropionic acid
-
pH 8.5, 37C
1.3
-
Dichloroacetate
-
pH 9.4, 30C
1.7
-
L-2-bromobutanoate
Q96XE7
pH 9.5, 70C
-
0.41
-
L-2-bromohexanoate
Q96XE7
pH 9.5, 70C
-
2.1
-
L-2-bromopropionate
Q96XE7
pH 9.5, 70C
-
0.37
-
L-2-chloropropionate
-
pH 9.5, 30C
0.37
-
L-2-chloropropionate
-
comparison with mutant enzymes
0.57
-
L-2-chloropropionate
-
pH 9.5, 37C
1.7
-
L-2-chloropropionate
Q96XE7
pH 9.5, 70C
3.8
-
L-2-chloropropionate
-
pH 10.5, 30C
19.5
-
L-2-chloropropionate
-
-
1.13
-
monobromoacetate
Q51645
pH 7.9, 30C
0.1
-
Monochloroacetate
Azotobacter sp.
-
pH 8.0, 30C
1
-
Monochloroacetate
-
pH 10.5, 30C
1.1
-
Monochloroacetate
-
pH 9.5, 30C
1.6
-
Monochloroacetate
-
pH 9.5, 37C
8
-
Monochloroacetate
-
pH 9.4, 30C
additional information
-
additional information
-
covalently immobilized L-2-haloacid dehalogenase subtype shows KM-value of 23.3 mM
-
additional information
-
additional information
-
comparison of KM of wild-type and mutant enzymes
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
47
-
L-2-chloropropionate
-
comparison with mutant enzymes
additional information
-
additional information
-
comparison of kcat of wild-type and mutant enzymes
-
additional information
-
additional information
-
single turnover
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.06
-
-
substrate (S)-2-chloropropionic acid, pH 7.3, 30C
0.7
-
Azotobacter sp.
-
coupled assay with lactate dehydrogenase
0.9
-
-
substrate: (S)-2-chloropropionic acid, pH 9.0, 22C, immobilized enzyme
3
-
-
substrate: (S)-2-chloropropionic acid, pH 9.0, 37C, immobilized enzyme
4.56
-
Q51645
-
6.3
-
-
substrate: (S)-2-chloropropionic acid, pH 9.0, 50C, immobilized enzyme
13.8
-
-
-
20
-
-
substrate: (S)-2-chloropropionic acid, pH 9.0, 50C, soluble enzyme
additional information
-
-
-
additional information
-
-
-
additional information
-
-
-
additional information
-
-
specific activities of diverse mutants
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
Q51645
-
8
9
Azotobacter sp.
-
-
9.5
10
Q96XE7
-
9.5
-
-
soluble and immobilized enzyme
10
11
Q52087
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
9
-
the enzyme reaction is retarded below pH 6.0 and the reaction is spontaneously over pH 9.0
8
10
Azotobacter sp.
-
-
8
10.5
-
pH 8.0: about 50% of maximal activity, pH 10.5: about 80% of maximal activity
9
11.5
Q96XE7
pH 9.0: about 45% of maximal activity, pH 11.5: about 70% of maximal activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
Azotobacter sp.
-
-
60
-
Q96XE7, -
-
70
-
Q96XE7
-
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
20
60
Azotobacter sp.
-
-
50
90
Q96XE7
50C: about 40% of maximal activity, 90C: about 80% of maximal activity
additional information
-
-
enzyme covalently immobilized is stable between 30C and 85C
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
-
Q52087
-
5.1
-
-
the mutation from an acidic to a basic amino acid leads to an alteration in the enzyme pI valiue from 5.1 to 6.0
5.5
-
Azotobacter sp.
-
isoelectric focusing
6.2
-
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
additional information
-
the growth of DEH 99 strain culture is not affected by salinity of 0-15%. Growth on 1,2-dichloroethane, 3-chloro-1,2-propanediol, 2,2-dichloropropionic acid, and 2,4,6-trichlorophenol medium
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Pseudomonas putida AJ1
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Polaromonas sp. (strain JS666 / ATCC BAA-500)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas sp. (strain YL)
Pseudomonas sp. (strain YL)
Pseudomonas sp. (strain YL)
Pseudomonas sp. (strain YL)
Ralstonia solanacearum (strain GMI1000)
Rhodococcus sp. (strain RHA1)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25230
-
-
calculated from amino acid sequence
25400
-
-
calculated from amino acid sequence
25600
-
-
SDS-PAGE
25680
-
-
calculated from amino acid sequence
25900
-
-
calculated from amino acid sequence
26180
-
-
calculated from amino acid sequence
27430
-
-
calculated from amino acid sequence
28000
-
Azotobacter sp.
-
gel filtration
28000
-
-
SDS-PAGE
31000
-
-
gel filtration
33600
-
-
calculated from amino acid sequence
34000
-
-
gel filtration
36000
-
-
gel filtration
38000
-
-
gel filtration
41000
-
-
gel filtration
49700
-
-
calculated apparent molecular weight
50000
-
-
gel filtration
54000
-
-
gel filtration
54000
-
-
SDS-PAGE
58000
-
Q51645
gel filtration
60000
-
-
gel filtration
64000
-
-
gel filtration
79000
-
Q52087
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 28000, SDS-PAGE
dimer
-
2 * 27000, SDS-PAGE
dimer
Q51645
2 * 27000, SDS-PAGE
dimer
-
2 * 31000 SDS-PAGE, 2 * 30868, calculated from amino acid sequence
dimer
-
2 * 29000 SDS-PAGE
dimer
-
the subunit consists of two structurally distinct domains: the core domain and the subdomain
dimer
-
2 * 24000, SDS-PAGE
dimer
-
2 * 28000, SDS-PAGE
-
dimer
-
2 * 29000 SDS-PAGE
-
dimer
-
2 * 27000, SDS-PAGE; 2 * 27000, SDS-PAGE; 2 * 28000, SDS-PAGE; the subunit consists of two structurally distinct domains: the core domain and the subdomain
-
homodimer
-
2 * 25600, SDS-PAGE
homodimer
Q96XE7, -
x-ray crystallography
homodimer
Pyrococcus horikoshii OT-3
-
2 * 25600, SDS-PAGE
-
homodimer
Sulfolobus tokodaii 7
-
x-ray crystallography
-
monomer
-
1 * 25000
monomer
Azotobacter sp.
-
1 * 29000 SDS-PAGE
monomer
-
1 * 31000, SDS-PAGE
tetramer
Q52087
1 * 26000, SDS-PAGE, 1 * 25687, calculated from amino acid sequence
tetramer
Pseudomonas putida AJ1
-
1 * 26000, SDS-PAGE, 1 * 25687, calculated from amino acid sequence
-
monomer
Azotobacter sp. RC26
-
1 * 29000 SDS-PAGE
-
additional information
-
docking and structure analysis of enzyme mutants K151A and D180A in complex with substrate (S)-2-chloropropionic acid, molecular dynamics, overview
additional information
-
docking and structure analysis of enzyme mutants K151A and D180A in complex with substrate (S)-2-chloropropionic acid, molecular dynamics, overview
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
; alpha/beta structure; mutant S175A
-
alpha/beta structure
-
crystal structures of enzyme complexed with monochloroacetate, L-2-chlorobutyrate, L-2-chloro-3-methylbutyrate, or L-2-chloro-4-methylvalerate are determined using the complex crystals prepared with the S175A mutant; mutant S175A
-
mutant S175A in complex with chloroacetate, 2-L-chlorobutyrate, 2-L-chloro-3-methylbutyrate, 2-L-chloro-4-methylvalerate, and L-2-chloropropionamide, all substrates bound have D-configuration, except for chloroacetate
-
oil-microbatch method, in 200 mM NaCl and 20 mM Tris-HCl pH 8.0, 25.5% (w/v) polyethylene glycol 4000, 0.17 M ammonium acetate, 15% (w/v) glycerol and 85 mM sodium acetate buffer, pH 4.6, at 20C
-
refined to 1.9 A resolution. Crystal is an orderdisorder twin by reticular merohedry with a twin index of 10
-
apoenzyme and enzyme in intermediate state with substrate L-2-monochloropropionate and monochloroacetate
Q60099
apoenzyme and enzyme in intermediate state with substrate L-2-monochloropropionate and monochloroacetate; three-dimensional structure of enzyme
-
three-dimensional structure of enzyme
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
10
Q96XE7
the enzyme tolerates extreme pH conditions ranging from 4 to 10
4.5
10.5
-
at 30C for 30 min
7.5
11
-
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
50
90
Q96XE7, -
the enzyme is stable to prolonged exposure to 50C, losing only 8% activity after 90 min incubation, as the temperature is increased the stability decreases, the enzyme is thermostable with a half-life of over 1 h at 70C
55
-
-
after 15 min loss of 50% activity
60
-
-
enzyme retains its full activity for 30 min
60
-
Azotobacter sp.
-
activity after 30 min reduced by 50%
60
-
-
recombinant in Escherichia coli
70
-
Q96XE7
4 h, enzyme remains fully active
80
-
Q96XE7
4 h, 10% loss of activity
90
-
Q96XE7
1 h, 70% loss of activity
additional information
-
Azotobacter sp.
-
activity and stability at different temperatures
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the enzyme retains approximately 30% of the overall activity after immobilization
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Acetone
Azotobacter sp.
-
100% activity at 10 mM
Acetone
Azotobacter sp. RC26
-
100% activity at 10 mM
-
acetonitrile
Q96XE7, -
in acetonitrile the activity is reduced by over 70% for any concentration tested
acetonitrile
Sulfolobus tokodaii 7
-
in acetonitrile the activity is reduced by over 70% for any concentration tested
-
chloroform
Azotobacter sp.
-
100% activity at 10 mM
chloroform
Azotobacter sp. RC26
-
100% activity at 10 mM
-
dithiothreitol
-
increases enzyme activity slight
dithiothreitol
-
increases enzyme activity slight
-
DMSO
-
enzyme active in 3 M DMSO at 30C for 5 min
DMSO
-
the stereochemistry of the product obtained from racemic 2-bromopropionic acid by the action of celite-immobilized disrupted cells in dimethylsulfoxide differs from that of the enantiomer obtained in an aqueous buffer
DMSO
Q96XE7, -
the enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% (v/v) DMSO, at higher concentrations of DMSO the activity is reduced to below 40% of normal
DMSO
Pseudomonas putida No. 109
-
the stereochemistry of the product obtained from racemic 2-bromopropionic acid by the action of celite-immobilized disrupted cells in dimethylsulfoxide differs from that of the enantiomer obtained in an aqueous buffer
-
DMSO
-
enzyme active in 3 M DMSO at 30C for 5 min
-
DMSO
Sulfolobus tokodaii 7
-
the enzyme is relatively stable to solvents with 25% activity lost when incubated for 1 h in 20% (v/v) DMSO, at higher concentrations of DMSO the activity is reduced to below 40% of normal
-
Ethanol
Q96XE7, -
in 10% (v/v) ethanol activity is still 40% of normal, further increases in ethanol concentration reduce the activity until at 50% (v/v) ethanol less than 10% activity is observed
Ethanol
Sulfolobus tokodaii 7
-
in 10% (v/v) ethanol activity is still 40% of normal, further increases in ethanol concentration reduce the activity until at 50% (v/v) ethanol less than 10% activity is observed
-
Methanol
Q96XE7, -
the activity in 10% (v/v) methanol is only 25% of normal, similar activity is observed for the higher concentrations of methanol
Methanol
Sulfolobus tokodaii 7
-
the activity in 10% (v/v) methanol is only 25% of normal, similar activity is observed for the higher concentrations of methanol
-
n-heptane
-
retaining 3.3% activity of that in water with substrate 2-chloropropionate
n-heptane
-
retaining 3.3% activity of that in water with substrate 2-chloropropionate
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-18C, pH 7.5, 6 months
-
-20C, pH 7.5 + 50% glycerol, 6 months, strain YL
-
50 mM Tris sulfate, pH 7.5 + 0.01% NaN3 at 25C or -30C, 30 days with remaining 60% activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
-
Azotobacter sp.
-
HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration
-
Ni-NTA column chromatography and gel filtration
Q96XE7, -
two-step method: heat treatment step that removes 90% of the impurities, followed by an acidification step that eliminates the remaining impurities. The easy purification procedure renders the production of this protein fast, cost effective, and suitable for large-scale production
Q96XE7
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli
Q51645
expression in Escherichia coli and Pseudomonas putida
-
gene dehII, DNA and amino acid sequence determination and analysis
-
phylogenetic analysis
-
expression in Escherichia coli
-
expression in Escherichia coli and Pseudomonas putida hosts by using broad-host-range vectors
-
expression in Escherichia coli
-
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells
-
expression in Escherichia coli
-
expressed in Escherichia coli
Q96XE7, -
expressed in Escherichia coli in a tag-free form
Q96XE7
expression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D11E
-
totally inactive in catalysis
D11N
-
totally inactive in catalysis
D11S
-
totally inactive in catalysis
N178D
-
defective in catalysis
D11E
Burkholderia cepacia MBA4
-
totally inactive in catalysis
-
D11N
Burkholderia cepacia MBA4
-
totally inactive in catalysis
-
D11S
Burkholderia cepacia MBA4
-
totally inactive in catalysis
-
D10N
-
Asn10 of the mutant enzyme is spontaneously deaminated to yield Asp, though slowly, causing increasing activity of the mutant preparation
D10N
Pseudomonas putida YL
-
Asn10 of the mutant enzyme is spontaneously deaminated to yield Asp, though slowly, causing increasing activity of the mutant preparation
-
D180A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
S118A
-
mutant with lower specific activity and higher KM-value
S175A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
D180A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
-
S118A
-
mutant with lower specific activity and higher KM-value
-
D180A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
-
S118A
-
mutant with lower specific activity and higher KM-value
-
S175A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
-
D181N
-
totally inactive in catalysis
additional information
-
mutant library with reduced activity
additional information
-
point mutations on 29 conserved charged and/or polar residues are generated and characterized
D181N
Burkholderia cepacia MBA4
-
totally inactive in catalysis
-
additional information
Burkholderia cepacia MBA4
-
mutant library with reduced activity; point mutations on 29 conserved charged and/or polar residues are generated and characterized
-
K151A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
additional information
-
diverse mutant types with higher KM-values and lower specific activity
additional information
-
mutant enzyme is constructed by introducing three lysyl residues at positions 11, 176 and 185 of enzyme by site-directed mutagenesis
additional information
-
-
additional information
-
mutants of all the 36 highly conserved charged and polar amino acid residues of enzyme, D10, D180, R41, K151, S118, S175, T14, Y157, and N177 play indispensable roles in the catalysis
K151A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
-
additional information
-
diverse mutant types with higher KM-values and lower specific activity; mutant enzyme is constructed by introducing three lysyl residues at positions 11, 176 and 185 of enzyme by site-directed mutagenesis
-
S175A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
-
additional information
-
-
-
K151A
-
site-directed mutagenesis of a residue that strongly interacts with the substrate
-
additional information
-
diverse mutant types with higher KM-values and lower specific activity; mutant enzyme is constructed by introducing three lysyl residues at positions 11, 176 and 185 of enzyme by site-directed mutagenesis; mutants of all the 36 highly conserved charged and polar amino acid residues of enzyme, D10, D180, R41, K151, S118, S175, T14, Y157, and N177 play indispensable roles in the catalysis
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
environmental protection
Azotobacter sp.
-
detoxification of halogenated herbicides, solvents and other xenobiotic compounds by immobilized enzyme
synthesis
Azotobacter sp.
-
production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry
environmental protection
Azotobacter sp. RC26
-
detoxification of halogenated herbicides, solvents and other xenobiotic compounds by immobilized enzyme
-
synthesis
Azotobacter sp. RC26
-
production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry; production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry
-
synthesis
-
production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry
synthesis
-
production of D-lactate in industry
synthesis
-
production of (S)-2-chloropropionic acid, which is used in the synthesis of optically active phenoxypropionic acid herbicides
synthesis
Pseudomonas putida AJ1
-
production of (S)-2-chloropropionic acid, which is used in the synthesis of optically active phenoxypropionic acid herbicides
-
synthesis
-
production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry
synthesis
-
L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents
synthesis
-
His-tagged L-2-haloacid dehalogenase subtype immobilized for production of D-lactate and D-chloropropionic acid
synthesis
-
L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents; production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry
-
synthesis
-
His-tagged L-2-haloacid dehalogenase subtype immobilized for production of D-lactate and D-chloropropionic acid
-
synthesis
-
L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents; L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents; L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents; production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry
-
analysis
-
the enzyme is covalently linked to an N-hydroxysuccinimidyl Sepharose resin to construct a highly specific sensor with long shelf life for the detection of L-2-haloacids