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Literature summary for 3.4.14.5 extracted from
- Catalytic properties and inhibition of proline-specific dipeptidyl peptidases II, IV and VII (2003), Biochem. J., 371, 525-532.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is a potential target for treatment of metabolic diseases | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in insect cells via baculovirus infection as secreted soluble enzyme with Asp29 to the end without any fusion tag | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyclohexylglycine thiazolidide | - |
Homo sapiens |  |
| Diprotin A | - |
Homo sapiens | |
| Ile cyanothiazolidide | - |
Homo sapiens | |
| Lys piperidide | - |
Homo sapiens | |
| Lys thiazolidide | - |
Homo sapiens | |
| threo-Ile thiazolidide | - |
Homo sapiens | |
| Val pyrrolidide | - |
Homo sapiens | |
| Val thiazolidide | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
Ala-Pro-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
| 0.034 | - |
norleucine-Pro-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
| 0.035 | - |
Lys-Pro-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
| 0.035 | - |
norleucine-Pro-rhodamine | pH 7.5, 37°C | Homo sapiens | |
| 0.063 | - |
Gly-Pro-7-amido-4-methylcoumarin | pH 7.5, 37°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | enzyme is involved in glucose metabolism in contributing to the regulation of glucagon family peptides | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from insect cells, rapidly to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Ala-Pro + 7-amino-4-methylcoumarin | - |
? | |
| Gly-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Gly-Pro + 7-amino-4-methylcoumarin | - |
? | |
| Lys-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | Lys-Pro + 7-amino-4-methylcoumarin | - |
? | |
| additional information | substrate specificity, positional scanning combinatorial dipeptide library profile shows that the enzyme is specific for Pro at P1 position and accepts a broad amino acid spectrum at P2 position with isoleucine and norleucine resulting in highest activity, norleucine-norleucine-rhodamine is no substrate, Gly-Ala-7-amido-4-methylcoumarin and Lys-Ala-7-amido-4-methylcoumarin are poor substrates | Homo sapiens | ? | - |
? | |
| additional information | enzyme is involved in glucose metabolism in contributing to the regulation of glucagon family peptides | Homo sapiens | ? | - |
? | |
| norleucine-Pro-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | norleucine-Pro + 7-amino-4-methylcoumarin | - |
? | |
| norleucine-Pro-rhodamine + H2O | - |
Homo sapiens | norleucine-Pro + rhodamine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dipeptidyl peptidase IV | - |
Homo sapiens |
| DPP-IV | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is essentially inactive at acidic pH | Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000001 | - |
Ile cyanothiazolidide | pH 7.5, 37° | Homo sapiens | |
| 0.000044 | - |
cyclohexylglycine thiazolidide | pH 7.5, 37° | Homo sapiens | |
| 0.0002 | - |
Lys thiazolidide | pH 7.5, 37° | Homo sapiens | |
| 0.00022 | - |
threo-Ile thiazolidide | pH 7.5, 37° | Homo sapiens | |
| 0.00028 | - |
Val thiazolidide | pH 7.5, 37° | Homo sapiens | |
| 0.00067 | - |
Lys piperidide | pH 7.5, 37° | Homo sapiens | |
| 0.00089 | - |
Val pyrrolidide | pH 7.5, 37° | Homo sapiens | |
| 0.0035 | - |
Diprotin A | pH 7.5, 37° | Homo sapiens | |
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