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Literature summary for 3.2.1.3 extracted from
- Purification and biochemical characterisation of glucoamylase from a newly isolated Aspergillus niger: relation to starch processing (2014), Food Chem., 161, 270-278.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 5 mM, about 30% residual activity | Aspergillus niger |  |
| Fe3+ | 5 mM, about 40% residual activity | Aspergillus niger | |
| Hg2+ | 5 mM, about 30% residual activity | Aspergillus niger | |
| additional information | not inhibitory at 5 mM: Ag2+, Ca2+, Zn2+, Mg2+ and Cd2+ and EDTA | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
starch | Km value is 0.33 mg/ml, pH 5.0, 40°C | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57151 | - |
x * 62000, SDS-PAGE, x * 57151, mass spectrometry | Aspergillus niger |
| 62000 | - |
x * 62000, SDS-PAGE, x * 57151, mass spectrometry | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 95 | - |
pH 5.0, 40°C | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Aspergillus niger | D-glucose + 4-nitrophenol | - |
? | |
| starch + H2O | - |
Aspergillus niger | D-glucose + ? | sole product | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE, x * 57151, mass spectrometry | Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life 20-25 min | Aspergillus niger |
| 80 | - |
half-life less than 3 min | Aspergillus niger |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 158.3 | - |
starch | pH 5.0, 40°C | Aspergillus niger | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus niger | isoelectric focusing | - |
4.4 |
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