Application | Comment | Organism |
---|---|---|
biotechnology | the enzyme might be useful in biotechnological processes | Thermochaetoides thermophila |
industry | the enzyme is used in industrial starch degradation | Thermochaetoides thermophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Thermochaetoides thermophila | |
Cu2+ | 12% inhibition at 1 mM | Thermochaetoides thermophila | |
Fe2+ | 80% inhibition at 1 mM | Thermochaetoides thermophila | |
Hg2+ | complete inhibition at 1 mM | Thermochaetoides thermophila | |
Mn2+ | 11% inhibition at 1 mM | Thermochaetoides thermophila | |
additional information | poor inhibition by Ba2+ at 1 mM | Thermochaetoides thermophila | |
Zn2+ | 25% inhibition at 1 mM | Thermochaetoides thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted to the culture medium | Thermochaetoides thermophila | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 20% activation at 1 mM | Thermochaetoides thermophila | |
K+ | 10% activation at 1 mM | Thermochaetoides thermophila | |
additional information | no effect by Mg2+ at 1 mM | Thermochaetoides thermophila | |
Na+ | 12% activation at 1 mM | Thermochaetoides thermophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
1 * 64000, SDS-PAGE | Thermochaetoides thermophila |
65400 | - |
gel filtration and native PAGE | Thermochaetoides thermophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermochaetoides thermophila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native soluble enzyme from culture medium 16.3 fold to homogeneity by ammonium sulfate fractionation, ion exchange and hydrophobic interaction chromatography, and gel filtration | Thermochaetoides thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
71.2 | - |
purified enzyme | Thermochaetoides thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Thermochaetoides thermophila | starch + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, SDS-PAGE | Thermochaetoides thermophila |
Synonyms | Comment | Organism |
---|---|---|
exo-1,4-alpha-D-glucanohydrolase | - |
Thermochaetoides thermophila |
glucoamylase | - |
Thermochaetoides thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermochaetoides thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | stable at, purified enzyme | Thermochaetoides thermophila |
65 | - |
60 min, 50% activity remaining of purified enzyme | Thermochaetoides thermophila |
70 | - |
30 min, 40% activity remaining of purified enzyme | Thermochaetoides thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Thermochaetoides thermophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 9 | 30% of maximal activity at pH 3.0 and pH 8.5, 50% of maximal activity at pH 3.5 and pH 7.0 | Thermochaetoides thermophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | stable at pH 3.0-8.0, about 50% remaining activity at pH 3.0 and pH 10.0, 30% at pH 2.5 and pH 11.0 | Thermochaetoides thermophila |