Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-ethyl-3-(dimethylaminopropyl) carbodiimide | inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose | Spodoptera frugiperda | |
1-ethyl-3-(dimethylaminopropyl) carbodiimide | inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose | Tenebrio molitor | |
D-galactose | - |
Spodoptera frugiperda | |
D-galactose | - |
Tenebrio molitor | |
gamma-1,4-galactonolactone | - |
Spodoptera frugiperda | |
gamma-1,4-galactonolactone | - |
Tenebrio molitor | |
melibiose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
melibiose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
raffinose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
raffinose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
stachyose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
stachyose | with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
Tris | - |
Spodoptera frugiperda | |
Tris | - |
Tenebrio molitor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
melibiose | 30°C, pH 5.8 | Spodoptera frugiperda | |
3.2 | - |
p-nitrophenyl alpha-D-galactopyranoside | 30°C, pH 5.0 | Tenebrio molitor | |
3.8 | - |
stachyose | 30°C, pH 5.0 | Tenebrio molitor | |
4.4 | - |
raffinose | 30°C, pH 5.0 | Tenebrio molitor | |
4.8 | - |
raffinose | 30°C, pH 5.8 | Spodoptera frugiperda | |
6.3 | - |
melibiose | 30°C, pH 5.0 | Tenebrio molitor | |
7.2 | - |
stachyose | 30°C, pH 5.8 | Spodoptera frugiperda | |
12 | - |
p-nitrophenyl alpha-D-galactopyranoside | 30°C, pH 5.8 | Spodoptera frugiperda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
2 * 46000 | Spodoptera frugiperda |
46000 | 49000 | gel filtration | Tenebrio molitor |
94000 | - |
ultracentrifugation | Spodoptera frugiperda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spodoptera frugiperda | - |
- |
- |
Tenebrio molitor | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Tenebrio molitor | - |
larva | - |
Spodoptera frugiperda | - |
midgut | of larva, three midgut alpha-galactosidases, SG1, SG2 and SG3 | Spodoptera frugiperda | - |
midgut | of larva, three midgut alpha-galactosidases, TG1, TG2 and TG3 | Tenebrio molitor | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
melibiose + H2O | 7.3% of Vmax with p-nitrophenyl alpha-D-galactopyranoside | Tenebrio molitor | D-galactose + D-glucose | - |
? | |
melibiose + H2O | 35.5% of Vmax with p-nitrophenyl alpha-D-galactopyranoside | Spodoptera frugiperda | alpha-D-galactose + D-glucose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Tenebrio molitor | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Spodoptera frugiperda | p-nitrophenol + alpha-D-galactose | - |
? | |
raffinose + H2O | 20% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside | Tenebrio molitor | sucrose + alpha-D-galactose | - |
? | |
raffinose + H2O | Vmax is 1.8fold higher than that with p-nitrophenyl alpha-D-galactopyranoside | Spodoptera frugiperda | sucrose + alpha-D-galactose | - |
? | |
stachyose + 2 H2O | 17.8% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside | Tenebrio molitor | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | Vmax is 2fold higher than that with p-nitrophenyl alpha-D-galactopyranoside | Spodoptera frugiperda | 2 alpha-D-galactose + sucrose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 46000 | Spodoptera frugiperda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Tenebrio molitor |
5.8 | - |
- |
Spodoptera frugiperda |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | pH 4.0: about 45% of maximal activity, pH 6.5: about 40% of maximal activity | Tenebrio molitor |
4.5 | 7.5 | pH 4.5: about 40% of maximal activity, pH 7.5: about 40% of maximal activity | Spodoptera frugiperda |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
30°C, 2 h, about 50% loss of activity | Tenebrio molitor |
4 | 6 | 30°C, 2 h, stable | Tenebrio molitor |
5 | 7 | stable | Spodoptera frugiperda |
7.5 | - |
30°C, 2 h, about 20% loss of activity | Tenebrio molitor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
D-galactose | 30°C, pH 5.0 | Tenebrio molitor | |
5.6 | - |
D-galactose | 30°C, pH 5.8 | Spodoptera frugiperda | |
6.6 | - |
stachyose | 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
7.7 | - |
melibiose | 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
9 | - |
raffinose | 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
9.2 | - |
gamma-1,4-galactonolactone | 30°C, pH 5.0 | Tenebrio molitor | |
9.6 | - |
Tris | 30°C, pH 5.0 | Tenebrio molitor | |
10.3 | - |
melibiose | 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Spodoptera frugiperda | |
11.6 | - |
stachyose | 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
12 | - |
Tris | 30°C, pH 5.8 | Spodoptera frugiperda | |
13.1 | - |
raffinose | 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate | Tenebrio molitor | |
43.1 | - |
gamma-1,4-galactonolactone | 30°C, pH 5.8 | Spodoptera frugiperda |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Tenebrio molitor | isoelectric focusing | - |
4.6 |
Tenebrio molitor | isoelectric focusing | - |
4.9 |
Spodoptera frugiperda | isoelectric focusing | - |
7.1 |
Spodoptera frugiperda | isoelectric focusing | - |
7.2 |