Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | complete inhibition at 5 and 10 mM | Carthamus tinctorius | |
Zn2+ | 22% residual activity at 10 mM | Carthamus tinctorius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 128% activity at 10 mM | Carthamus tinctorius | |
additional information | not affected by Mg2+ and Cu2+ | Carthamus tinctorius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
SDS-PAGE | Carthamus tinctorius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carthamus tinctorius | - |
safflower | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, beta-cyclodextrin-Sepharose column chromatography | Carthamus tinctorius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Carthamus tinctorius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.89 | - |
crude extract | Carthamus tinctorius |
246.5 | - |
after 131.52fold purification | Carthamus tinctorius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Carthamus tinctorius | malto-oligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-1-4 D-glucan glucanohydrolase | - |
Carthamus tinctorius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Carthamus tinctorius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | - |
Carthamus tinctorius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | the enzyme loses 50% of its activity after 10 min incubation at 70°C and is completely denatured at 100°C | Carthamus tinctorius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Carthamus tinctorius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | - |
Carthamus tinctorius |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
at pH values higher than 9.0 enzymatic activity drops sharply to 50% of maximal activity | Carthamus tinctorius |