Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.1 extracted from

  • Kato, K.; Sugimoto, T.; Amemura, A.; Harada, T.
    A Pseudomonas intracellular amylase with high activity on maltodextrins and cyclodextrins (1975), Biochim. Biophys. Acta, 391, 96-108.
    View publication on PubMed
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 2 mM, complete inhibition Pseudomonas sp.
Cd2+ 2 mM, 65% inhibition Pseudomonas sp.
Cu2+ 2 mM, complete inhibition Pseudomonas sp.
Fe2+ 2 mM, 49% inhibition Pseudomonas sp.
Hg2+ 2 mM, complete inhibition Pseudomonas sp.
Pb2+ 2 mM, 53% inhibition Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas sp.
2
-
 maltotriose
-
 Pseudomonas sp.
2.1
-
 maltotetraose
-
 Pseudomonas sp.
3.6
-
 cyclomaltohexaose
-
 Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Pseudomonas sp. 5622
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
 x * 96000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 MS1
-
Pseudomonas sp. MS1
-
 MS1
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Pseudomonas sp. additional information
-
 ?
amylopectin + H2O
-
 Pseudomonas sp. MS1 additional information
-
 ?
amylose + H2O
-
 Pseudomonas sp. additional information
-
 ?
amylose + H2O
-
 Pseudomonas sp. MS1 additional information
-
 ?
cyclomaltohexaose + H2O
-
 Pseudomonas sp. ?
-
 ?
cyclomaltohexaose + H2O
-
 Pseudomonas sp. MS1 ?
-
 ?
maltopentaose + H2O
-
 Pseudomonas sp. additional information glucose + maltose + maltotriose + a slight amount of maltotetraose ?
maltotetraose + H2O
-
 Pseudomonas sp. additional information glucose + maltose + maltotriose ?
maltotriose + H2O
-
 Pseudomonas sp. maltose + D-glucose
-
 ?
maltotriose + H2O
-
 Pseudomonas sp. MS1 maltose + D-glucose
-
 ?
starch + H2O soluble starch Pseudomonas sp. additional information
-
 ?
starch + H2O soluble starch Pseudomonas sp. MS1 additional information
-
 ?

Subunits

Subunits Comment Organism
? x * 96000, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 30 min, stable Pseudomonas sp.
60
-
 30 min, complete loss of activity Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Pseudomonas sp.