General Stability | Organism |
---|---|
Ca2+ stabilizes | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 2 mM, 61.2% inhibition | Bacillus subtilis | |
Al3+ | 2 mM, 38.7% inhibition | Bacillus subtilis | |
Cd2+ | 2 mM, 43% inhibition | Bacillus subtilis | |
Cu2+ | 2 mM, almost complete inhibition | Bacillus subtilis | |
EDTA | 0.1 mM, 61.3% inhibition | Bacillus subtilis | |
Fe3+ | 2 mM, almost complete inhibition | Bacillus subtilis | |
Hg2+ | 2 mM, almost complete inhibition | Bacillus subtilis | |
Mn2+ | 2 mM, almost complete inhibition | Bacillus subtilis | |
Pb2+ | 2 mM, 38% inhibition | Bacillus subtilis | |
Zn2+ | 2 mM 30.3% inhibition | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
amylose | - |
Bacillus subtilis | |
0.12 | - |
maltopentaose | - |
Bacillus subtilis | |
0.198 | - |
maltotriose | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
x * 68000, SDS-PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 65 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.8 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + H2O | - |
Bacillus subtilis | additional information | - |
? | |
glycogen + H2O | - |
Bacillus subtilis | additional information | - |
? | |
maltopentaose + H2O | - |
Bacillus subtilis | additional information | - |
? | |
maltotriose + H2O | - |
Bacillus subtilis | maltose + D-glucose | - |
? | |
maltotriose + H2O | - |
Bacillus subtilis 65 | maltose + D-glucose | - |
? | |
p-nitrophenyl alpha-D-maltoside + H2O | - |
Bacillus subtilis | p-nitrophenol + maltose | - |
? | |
starch + H2O | digestion of raw potato starch is almost as far as that of corn starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
starch + H2O | sweet potato starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
starch + H2O | corn starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
starch + H2O | waxy corn starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
starch + H2O | wheat starch | Bacillus subtilis | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
starch + H2O | digestion of raw potato starch is almost as far as that of corn starch | Bacillus subtilis 65 | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
starch + H2O | sweet potato starch | Bacillus subtilis 65 | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
starch + H2O | corn starch | Bacillus subtilis 65 | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? | |
starch + H2O | waxy corn starch | Bacillus subtilis 65 | additional information | glucose + maltose + small amounts of maltooligosaccharides ranging from maltotriose to maltoheptaose | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 68000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 80 | 40°C: about 30% of maximal activity, 80°C: about 40% of maximal activity | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, unstable above | Bacillus subtilis |
65 | - |
5 min, 95% loss of activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | pH 4.0: about 70% of maximal activity, pH 10.0: about 40% of maximal activity | Bacillus subtilis |
6 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 4°C, 24 h, stable | Bacillus subtilis |