General Stability | Organism |
---|---|
glycerol, 25%, is required for stability during purification for the enzyme form PO-FAT | Diploknema butyracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0034 | - |
stearoyl-[acyl-carrier protein] | enzyme form PO-FAT, pH 9.0, 37°C | Diploknema butyracea | |
0.0036 | - |
palmitoyl-[acyl-carrier protein] | enzyme form PO-FAT, pH 9.0, 37°C | Diploknema butyracea | |
0.008 | - |
oleoyl-[acyl-carrier protein] | enzyme form PO-FAT, pH 9.0, 37°C | Diploknema butyracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Diploknema butyracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleoyl-[acyl-carrier protein] + H2O | Diploknema butyracea | - |
oleic acid + acyl-carrier protein | - |
? | |
palmitoyl-[acyl-carrier protein] + H2O | Diploknema butyracea | - |
palmitic acid + acyl-carrier protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Diploknema butyracea | - |
i.e. Brassica butyracea, 2 enzyme forms: a palmitoyl-/oleoyl-[acyl carrier protein] specific and a oleoyl-[acyl carrier protein] specific one, i.e. PO-FAT and LC-FAT, respectively | - |
Purification (Comment) | Organism |
---|---|
enzyme form PO-FAT, 499fold | Diploknema butyracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower bud | - |
Diploknema butyracea | - |
leaf | young | Diploknema butyracea | - |
seed | developing, period from 45 to 93 days after flowering | Diploknema butyracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Diploknema butyracea |
0.69 | - |
seed 65 days after flowering, enzyme form PO-FAT | Diploknema butyracea |
192 | - |
purified enzyme form PO-FAT | Diploknema butyracea |
Storage Stability | Organism |
---|---|
-20°C, enzyme form PO-FAT, ammonium sulfate pellet, stable | Diploknema butyracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleoyl-[acyl-carrier protein] + H2O | - |
Diploknema butyracea | oleic acid + acyl-carrier protein | - |
? | |
oleoyl-[acyl-carrier protein] + H2O | preferred substrate of the oleoyl-[acyl carrier protein] specific enzyme form LC-FAT | Diploknema butyracea | oleic acid + acyl-carrier protein | - |
? | |
palmitoyl-[acyl-carrier protein] + H2O | - |
Diploknema butyracea | palmitic acid + acyl-carrier protein | - |
? | |
palmitoyl-[acyl-carrier protein] + H2O | preferred substrate of the palmitoyl-/oleoyl-[acyl carrier protein] specific enzyme form PO-FAT | Diploknema butyracea | palmitic acid + acyl-carrier protein | - |
? | |
stearoyl-[acyl-carrier protein] + H2O | - |
Diploknema butyracea | stearic acid + acyl-carrier protein | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42500-44000, SDS-PAGE | Diploknema butyracea |
Synonyms | Comment | Organism |
---|---|---|
12:0-ACP thioesterase | - |
Diploknema butyracea |
12:0-acyl-carrier protein thioesterase | - |
Diploknema butyracea |
14:0-ACP thioesterase | - |
Diploknema butyracea |
14:0-acyl-carrier protein thioesterase | - |
Diploknema butyracea |
16:0-ACP thioesterase | - |
Diploknema butyracea |
16:0-acyl-carrier protein thioesterase | - |
Diploknema butyracea |
18:0-ACP thioesterase | - |
Diploknema butyracea |
18:0-acyl-carrier protein thioesterase | - |
Diploknema butyracea |
acyl-ACP-hydrolase | - |
Diploknema butyracea |
acyl-acyl carrier protein hydrolase | - |
Diploknema butyracea |
acyl-acyl carrier protein thioesterase | - |
Diploknema butyracea |
acyl-[acyl-carrier-protein] hydrolase | - |
Diploknema butyracea |
AH | - |
Diploknema butyracea |
BTE | - |
Diploknema butyracea |
C12:0 ACP TE | - |
Diploknema butyracea |
C18:1-ACP thioesterase | - |
Diploknema butyracea |
Deubiquitinating enzyme 14 | - |
Diploknema butyracea |
FAT | - |
Diploknema butyracea |
fatty acyl-ACP thioesterase | - |
Diploknema butyracea |
hydrolase, oleoyl-[acyl carrier protein] | - |
Diploknema butyracea |
LC-FAT | oleoyl-[acyl carrier protein] specific | Diploknema butyracea |
oleoyl-ACP thioesterase | - |
Diploknema butyracea |
oleoyl-acyl carrier protein thioesterase | - |
Diploknema butyracea |
OTE | - |
Diploknema butyracea |
PATE | - |
Diploknema butyracea |
PO-FAT | palmitoyl-/oleoyl-[acyl carrier protein] specific | Diploknema butyracea |
STE | - |
Diploknema butyracea |
Ubiquitin thiolesterase 14 | - |
Diploknema butyracea |
Ubiquitin-specific processing protease 14 | - |
Diploknema butyracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Diploknema butyracea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
15 min, enzyme form LC-FAT is mostly inactivated, while enzyme form PO-FAT is activated by 15-20% | Diploknema butyracea |
70 | - |
15 min, enzyme form PO-FAT is heat-stable, after 45 min the enzyme retains 30% of the original activity | Diploknema butyracea |
80 | - |
15 min, 30% remaining activity | Diploknema butyracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
enzyme form PO-FAT | Diploknema butyracea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Diploknema butyracea | enzyme form LC-FAT, chromatofocusing | - |
8.2 |
Diploknema butyracea | enzyme form PO-FAT, chromatofocusing | - |
9.7 |