Information on EC 3.1.2.14 - oleoyl-[acyl-carrier-protein] hydrolase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.1.2.14
-
RECOMMENDED NAME
GeneOntology No.
oleoyl-[acyl-carrier-protein] hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
oleoyl-[acyl-carrier protein] + H2O = [acyl-carrier protein] + oleate
show the reaction diagram
-
-
-
-
oleoyl-[acyl-carrier protein] + H2O = [acyl-carrier protein] + oleate
show the reaction diagram
catalytic residues are Asn227, Cys264 and His229, forming a papain-like catalytic triad
Q42562
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of thioester
-
-
-
-
hydrolysis of thioester
-
-
hydrolysis of thioester
-
-
hydrolysis of thioester
Elaeis sp.
-
-
hydrolysis of thioester
-, Q9FT16
-
hydrolysis of thioester
-
-
hydrolysis of thioester
-
-
hydrolysis of thioester
Q9SQI3
-
hydrolysis of thioester
-
-
hydrolysis of thioester
-
-
PATHWAY
KEGG Link
MetaCyc Link
acyl-ACP thioesterase pathway
-
cis-vaccenate biosynthesis
-
Fatty acid biosynthesis
-
Metabolic pathways
-
oleate biosynthesis I (plants)
-
palmitate biosynthesis I (animals and fungi)
-
palmitate biosynthesis II (bacteria and plants)
-
palmitoleate biosynthesis I
-
palmitoleate biosynthesis II
-
petroselinate biosynthesis
-
stearate biosynthesis II (bacteria and plants)
-
SYSTEMATIC NAME
IUBMB Comments
oleoyl-[acyl-carrier protein] hydrolase
Acts on acyl-carrier-protein thioesters of fatty acids from C12 to C18, but the derivative of oleic acid is hydrolysed much more rapidly than any other compound tested.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
12:0-ACP thioesterase
-
-
-
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
Q9FT16
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
Elaeis sp.
-
-
12:0-ACP thioesterase
Q9SQI3
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
-
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
Q9FT16
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
Elaeis sp.
-
-
12:0-acyl-carrier protein thioesterase
Q9SQI3
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
14:0-ACP thioesterase
-
-
-
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
Q9FT16
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
Elaeis sp.
-
-
14:0-ACP thioesterase
Q9SQI3
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
-
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
Q9FT16
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
Elaeis sp.
-
-
14:0-acyl-carrier protein thioesterase
Q9SQI3
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
16:0-ACP thioesterase
-
-
-
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
Q9FT16
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
Elaeis sp.
-
-
16:0-ACP thioesterase
Q9SQI3
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
-
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
Q9FT16
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
Elaeis sp.
-
-
16:0-acyl-carrier protein thioesterase
Q9SQI3
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
18:0-ACP thioesterase
-
-
-
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
Q9FT16
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
Elaeis sp.
-
-
18:0-ACP thioesterase
Q9SQI3
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
-
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
Q9FT16
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
Elaeis sp.
-
-
18:0-acyl-carrier protein thioesterase
Q9SQI3
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
acyl-ACP TE
A7XZV1
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
Q9FT16
-
acyl-ACP thioesterase
Q39473
myristic acid biased
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
A0PGT6
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
Helianthus annuus RHA-274
-
-
-
acyl-ACP thioesterase
A7XZV1
-
acyl-ACP thioesterase
A9XK92
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
Q41634
lauric acid biased
acyl-ACP thioesterase FatA type
D5FFZ2
-
acyl-ACP thioesterase FatB type
D2E6T0
-
acyl-ACP-hydrolase
-
-
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
Q9FT16
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
Elaeis sp.
-
-
acyl-ACP-hydrolase
Q9SQI3
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-thioesterase
-
-
acyl-acyl carrier protein (ACP) thioesterase
A9XK92
-
acyl-acyl carrier protein hydrolase
-
-
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
Q9FT16
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
Elaeis sp.
-
-
acyl-acyl carrier protein hydrolase
Q9SQI3
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
Q9FT16
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
Helianthus annuus RHA-274
-
-
-
acyl-acyl carrier protein thioesterase
A7XZV1
-
acyl-acyl carrier protein thioesterase
A1XAM4
-
acyl-acyl carrier protein thioesterase
Populus tomentosa Carr
A1XAM4
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase B1
-
-
acyl-acyl carrier protein-thioesterase
-
-
acyl-acyl-carrier protewin thioesterase
-
-
Acyl-[acyl-carrier protein] hydrolase
-
-
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
Q9FT16
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
Elaeis sp.
-
-
acyl-[acyl-carrier-protein] hydrolase
Q9SQI3
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acylACP thioesterase
A9XUG5
-
acylacyl carrier protein (ACP) thioesterase
A9XUG5
-
AH
-
-
-
-
AH
Q9FT16
-
AH
Elaeis sp.
-
-
BTE
-
-
-
-
BTE
Q9FT16
-
BTE
Elaeis sp.
-
-
BTE
Q9SQI3
-
C12:0 ACP TE
-
-
-
-
C12:0 ACP TE
Q9FT16
-
C12:0 ACP TE
-
-
C12:0 ACP TE
-
-
C12:0 ACP TE
-
-
C12:0 ACP TE
Elaeis sp.
-
-
C12:0 ACP TE
Q9SQI3
-
C12:0 ACP TE
-
-
C12:0 ACP TE
-
-
C12:0 ACP TE
-
-
C18:1-ACP thioesterase
-
-
-
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
Q9FT16
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
Elaeis sp.
-
-
C18:1-ACP thioesterase
Q9SQI3
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
-
CcFATB1
Q2Q1N8
-
CcFATB2
Q2Q1N7
-
Deubiquitinating enzyme 14
-
-
-
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
Q9FT16
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
Elaeis sp.
-
-
Deubiquitinating enzyme 14
Q9SQI3
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
-
FatA
A9XK92
-
FatA-type thioesterase
-
-
FatA-type thioesterase
Helianthus annuus RHA-274
-
-
-
FatA1 thioesterase
-
-
FatA1 thioesterase
Helianthus annuus RHA-274
-
-
-
FatB
A9XUG5
-
FATB1
Q2Q1N8
-
FATB1
A7XZV1
-
fatty acyl-ACP thioesterase
-
-
fatty acyl-ACP thioesterase
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
Q9FT16
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
Elaeis sp.
-
-
hydrolase, oleoyl-[acyl carrier protein]
Q9SQI3
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
LC-FAT
-
oleoyl-[acyl carrier protein] specific
MbFatB
A0PGT6
-
MlFatB
A0PGT7
-
oleoyl-ACP thioesterase
-
-
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
Q9FT16
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
Q9XEL9
-
oleoyl-ACP thioesterase
Elaeis sp.
-
-
oleoyl-ACP thioesterase
Q9SQI3
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
-
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
Q9FT16
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
Elaeis sp.
-
-
oleoyl-acyl carrier protein thioesterase
Q9SQI3
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
OTE
-
-
-
-
OTE
Q9FT16
-
OTE
Elaeis sp.
-
-
OTE
Q9SQI3
-
palmitoyl-ACP thioesterase
Q9SQI3
-
palmitoyl-acyl carrier protein thioesterase
Elaeis sp.
-
-
palmitoyl-acyl carrier protein thioesterase
Q9SQI3
-
PATE
-
-
-
-
PATE
Q9FT16
-
PATE
Elaeis sp.
-
-
PATE
Q9SQI3
-
PO-FAT
-
palmitoyl-/oleoyl-[acyl carrier protein] specific
PtFATB
A1XAM4
-
PtFATB
Populus tomentosa Carr
A1XAM4
-
-
saturated acyl ACP thioesterase type B
A7XZV1
-
seed-specific acyl-acyl carrier protein thioesterase
Q2Q1N8
-
SO-Fat
A0PGT7
-
STE
-
-
-
-
STE
Q9FT16
-
STE
Elaeis sp.
-
-
STE
Q9SQI3
-
stearoyl/oleoyl specific fatty acyl-acyl carrier protein thioesterase
A0PGT7
-
stearoyl/oleoyl-specific Fat
A0PGT7
-
Ubiquitin thiolesterase 14
-
-
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
Q9FT16
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
Elaeis sp.
-
-
Ubiquitin thiolesterase 14
Q9SQI3
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin-specific processing protease 14
-
-
-
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
Q9FT16
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
Elaeis sp.
-
-
Ubiquitin-specific processing protease 14
Q9SQI3
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
-
CAS REGISTRY NUMBER
COMMENTARY
105669-84-9
-
68009-83-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
At FatA1 and At FatB1
-
-
Manually annotated by BRENDA team
ecotype Col-2
-
-
Manually annotated by BRENDA team
gene fatB
Q42562
Uniprot
Manually annotated by BRENDA team
isozyme FatA and FatB
-
-
Manually annotated by BRENDA team
cv. Pusa Bold
SwissProt
Manually annotated by BRENDA team
Bn FatA1
-
-
Manually annotated by BRENDA team
cv. Quantum
-
-
Manually annotated by BRENDA team
Br FatA1
-
-
Manually annotated by BRENDA team
safflower, Ct FatA1 and Ct FatA2
-
-
Manually annotated by BRENDA team
Cs FatA1
-
-
Manually annotated by BRENDA team
isozyme FatA
-
-
Manually annotated by BRENDA team
Coriandrum sativum Cs FatA1
Cs FatA1
-
-
Manually annotated by BRENDA team
isozymes AH1, AH2, AH3
-
-
Manually annotated by BRENDA team
var. mesostemon
SwissProt
Manually annotated by BRENDA team
Ch FatA1 and Ch FatB1 and Ch FatB2
-
-
Manually annotated by BRENDA team
Cl FatB1
-
-
Manually annotated by BRENDA team
fragment of precursor
SwissProt
Manually annotated by BRENDA team
i.e. Brassica butyracea, 2 enzyme forms: a palmitoyl-/oleoyl-[acyl carrier protein] specific and a oleoyl-[acyl carrier protein] specific one, i.e. PO-FAT and LC-FAT, respectively
-
-
Manually annotated by BRENDA team
Elaeis sp.
oil palm
-
-
Manually annotated by BRENDA team
Gm FatA1 and Gm FatA2
-
-
Manually annotated by BRENDA team
cv. Paymaster HS26, gene FatB
SwissProt
Manually annotated by BRENDA team
line RHA-274 with normal fatty acid content and high-palmitic seed oil line CAS-5, 2 isozymes: FatA type and FatB type
-
-
Manually annotated by BRENDA team
strain RHA-274, gene fatA1
-
-
Manually annotated by BRENDA team
Helianthus annuus RHA-274
strain RHA-274, gene fatA1
-
-
Manually annotated by BRENDA team
fragment of precursor
UniProt
Manually annotated by BRENDA team
var. Fuerte, avocado
-
-
Manually annotated by BRENDA team
Populus tomentosa Carr
Carr
SwissProt
Manually annotated by BRENDA team
Um FatB1
-
-
Manually annotated by BRENDA team
bay, UcFatB1
-
-
Manually annotated by BRENDA team
Uc FatB1 and Uc FatB2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
deduced amino acid sequence displays high homology to choroplastic palmitoyl-acyl carrier protein thioesterases from other plants
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
role in controlling the acyl flux through the eucaryotic and procaryotic pathway in plants
-
-
-
decanoyl-[acyl-carrier protein] + H2O
decanoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
decanoyl-[acyl-carrier protein] + H2O
decanoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
-
-
dodecenoyl-[acyl-carrier protein] + H2O
dodecenoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-CoA + H2O
lauric acid + CoA
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
low activity
-
?
myristoleoyl-[acyl-carrier protein] + H2O
myristoleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
myristoleoyl-[acyl-carrier protein] + H2O
myristoleic acid + acyl-carrier protein
show the reaction diagram
-
low activity
-
?
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
-
-
-
-
-
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
-
-
?
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
-
low activity
-
?
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
-
activity of the TE 3-2 clone expressed in E. coli
-
?
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
-
activity of the medium-chain acyl-ACP thioesterase
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-, Q9FT16
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
activity of the TE 3-2 clone expressed in E. coli
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
activity of the oleoyl-ACP thioesterase
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
activity of the clones pCGN3270 and pCGN3271 expressed in E. coli
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
most reactive substrate
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of FatA type isozyme, low activity with FatB type isozyme
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of recombinant chimeric mutant FatAB
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of the oleoyl-[acyl carrier protein] specific enzyme form LC-FAT
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
substrates are long chain acyl-acyl carrier proteins, role in fatty acid biosynthesis
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
A9XK92, A9XUG5
high efficiency of FatB
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
A9XK92, A9XUG5
maximum catalytic efficiency of FatA
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleate + acyl-carrier protein
show the reaction diagram
-
substrates are long chain acyl-acyl carrier proteins
-
?
oleoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
endpoint of de novo fatty acid synthesis in plastids
-
-
-
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
-
preferred substrate
-
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
-
preferred substrate
-
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
Q9XEL9
preferred substrate
-
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
-
preferred substrate of wild-type and recombinant enzyme
-
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
Helianthus annuus RHA-274
-
preferred substrate, preferred substrate of wild-type and recombinant enzyme
-
-
?
oleoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + oleic acid
show the reaction diagram
-
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + oleic acid
show the reaction diagram
D2E6T0, D5FFZ2
-
-
-
?
oleoyl-[acyl-carrier-protein] + H2O
oleic acid + acyl-carrier-protein
show the reaction diagram
A0PGT7, -
88.2% of the activity with stearoyl-[acyl-carrier-protein]
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + palmitoleate
show the reaction diagram
Helianthus annuus, Helianthus annuus RHA-274
-
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
show the reaction diagram
A9XK92, A9XUG5
high catalytic efficiency of FatA
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
show the reaction diagram
A9XK92, A9XUG5
high efficiency of FatB
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + palmitoleic acid
show the reaction diagram
D2E6T0, D5FFZ2
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
best substrate
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
activity of the TE 3-2 clone expressed in E. coli
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
10% of the activity with oleoyl-[acyl-carrier-protein]
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
activity of the clone pCGN3271 expressed in E. coli
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of FatB type isozyme
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of recombinant chimeric mutant FatBA, isozyme FatB prefers [acyl-carrier-protein]-substrate of spinach before the Escherichia coli derived
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of the palmitoyl-/oleoyl-[acyl carrier protein] specific enzyme form PO-FAT
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
enzyme plays an important role in the control of seed-oil composition, determination of fatty acid chain growth
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
A9XK92, A9XUG5
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
best substrate
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
best substrate, role in fatty acid biosynthesis in flowers and seeds, overview about the fatty acid composition of the plants
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
A7XZV1, -
FATB1 is a palmitoyl-acyl-acyl carrier protein thioesterase. Seed-specific expression of FATB1 in Arabidopsis leads to a 3-4fold increase in the proportion of accumulated palmitate (up to 40.9 mol%) and moderate increases in the proportions of other saturated fatty acids, with associated reductions in the proportions of unsaturated fatty acids. Residues H317 and C352 are essential for catalytic activity
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + palmitate
show the reaction diagram
-
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + palmitic acid
show the reaction diagram
D2E6T0, D5FFZ2
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + palmitic acid
show the reaction diagram
-
relative specific activity to oleoyl-[acyl-carrier protein]: 1.8%
-
-
?
palmitoyl-[acyl-carrier-protein] + H2O
palmitic acid + acyl-carrier-protein
show the reaction diagram
A0PGT7, -
12% of the activity with stearoyl-[acyl-carrier-protein]
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
activity of the TE 3-2 clone expressed in E. coli
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
10% of the activity with oleoyl-[acyl-carrier-protein]
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
activity of the clone pCGN3271 expressed in E. coli
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
highly specific for, leaf epidermis enzyme
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of FatB type isozyme, low activity with FatA type isozyme
-
?
stearoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
synthesis of fatty acids for wax biosynthesis
-
-
-
stearoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + stearate
show the reaction diagram
-
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + stearate
show the reaction diagram
-
low activity
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearate + acyl-carrier protein
show the reaction diagram
A9XK92, A9XUG5
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + stearic acid
show the reaction diagram
D2E6T0, D5FFZ2
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + stearic acid
show the reaction diagram
-
relative specific activity to oleoyl-[acyl-carrier protein]: 35%
-
-
?
stearoyl-[acyl-carrier-protein] + H2O
stearic acid + acyl-carrier-protein
show the reaction diagram
A0PGT7, -
-
-
-
?
undecanoyl-[acyl-carrier protein] + H2O
undecanoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
activity of the medium-chain acyl-ACP thioesterase
-
?
additional information
?
-
Q9SQI3
substrate specificity
-
?
additional information
?
-
-
Fat A thioesterases are specific for unsaturated substrates e.d. 18:1-ACP, FatB thioesterases form two groups are either specific for saturated acyl chain of C-8 to C-14, i.e. medium-chain length, or specific for C-14 to C-18 acyl chain length
-
-
-
additional information
?
-
-
the reaction rate increases with increasing chain length of acyl from C-12 to C-18
-
-
-
additional information
?
-
-
substrate specificities of isozymes and chimeric mutants, kcat is strongly influenced by substrate chain length and double bonds
-
?
additional information
?
-
Elaeis sp.
-
the recombinant enzyme hydrolyzes medium- and long-chain acyl-CoAs with a 10fold lower activity compared to acyl-[acyl-carrier-protein]s
-
?
additional information
?
-
-
the substrate specificity determinants are located at the N-terminus of the isozymes, kcat is strongly influenced by substrate chain length and double bonds
-
?
additional information
?
-
-, Q9FT16
enzyme is the chain-length-determining enzyme in de novo biosynthesis of plant fatty acids
-
?
additional information
?
-
-
the recombinant enzyme mediates polyhydroxyalkanoate biosynthesis to expressing Escherichia coli, biosynthesis pathway overview
-
?
additional information
?
-
-
the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds, substrate specificity of recombinant enzyme, the enzyme shows preference for monounsaturated acyl-ACPs
-
-
-
additional information
?
-
-
the enzyme is long-chain fatty acid specific
-
-
-
additional information
?
-
A9XK92, A9XUG5
FatA enzyme displays low levels of activity on saturated stearoyl- and palmitoyl-[acyl-carrier protein] derivatives
-
-
-
additional information
?
-
Helianthus annuus RHA-274
-
the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds, substrate specificity of recombinant enzyme, the enzyme shows preference for monounsaturated acyl-ACPs
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-, Q9FT16
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
role in fatty acid biosynthesis
-
?
oleoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
endpoint of de novo fatty acid synthesis in plastids
-
-
-
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
-
preferred substrate
-
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
Q9XEL9
preferred substrate
-
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
Helianthus annuus RHA-274
-
preferred substrate
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
enzyme plays an important role in the control of seed-oil composition, determination of fatty acid chain growth
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
best substrate
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
best substrate, role in fatty acid biosynthesis in flowers and seeds, overview about the fatty acid composition of the plants
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
stearoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
synthesis of fatty acids for wax biosynthesis
-
-
-
acyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
role in controlling the acyl flux through the eucaryotic and procaryotic pathway in plants
-
-
-
additional information
?
-
-, Q9FT16
enzyme is the chain-length-determining enzyme in de novo biosynthesis of plant fatty acids
-
?
additional information
?
-
-
the recombinant enzyme mediates polyhydroxyalkanoate biosynthesis to expressing Escherichia coli, biosynthesis pathway overview
-
?
additional information
?
-
Helianthus annuus, Helianthus annuus RHA-274
-
the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds
-
-
-
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
-
diethyldicarbonate
-
-
N-ethylmaleimide
-
-
oleoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
palmitoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
stearoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
additional information
-
no inhibition by phenylmethylsulfonylfluoride
-
additional information
-
no inhibition by phenylmethylsulfonylfluoride
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0006
-
(9Z)-hexadec-9-enoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.0023
-
decanoyl-[acyl-carrier protein]
-
-
0.0031
-
decanoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.004
-
decanoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0049
-
dodecanoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.0146
-
lauroyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0029
-
myristoleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.0139
-
myristoleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.0028
-
myristoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.004
-
myristoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.0059
-
myristoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0067
-
myristoyl-[acyl-carrier protein]
-
-
0.0089
-
myristoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.0024
-
octanoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.029
-
octanoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.00008
-
oleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.00042
-
oleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00067
-
oleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00074
-
oleoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 30 days after flowering
0.0008
-
oleoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 15 days after flowering
0.0013
-
oleoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0014
-
oleoyl-[acyl-carrier protein]
-
pH 8.0, 22C
0.0021
-
oleoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.0026
-
oleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.00297
-
oleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.0031
-
oleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.004
-
oleoyl-[acyl-carrier protein]
-
pH 9, 37C
0.0048
-
oleoyl-[acyl-carrier protein]
-
-
0.0074
-
oleoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.008
-
oleoyl-[acyl-carrier protein]
-
enzyme form PO-FAT, pH 9.0, 37C
3
-
oleoyl-[acyl-carrier protein]
-
pH 9.0, 35C
0.00035
-
palmitoleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00035
-
palmitoleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.00086
-
palmitoleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.0026
-
palmitoleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.0049
-
palmitoleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.00551
-
palmitoleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.00028
-
palmitoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 15 days after flowering
0.00053
-
palmitoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 30 days after flowering
0.00054
-
palmitoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00056
-
palmitoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00096
-
palmitoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.0013
-
palmitoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.0036
-
palmitoyl-[acyl-carrier protein]
-
-
0.0036
-
palmitoyl-[acyl-carrier protein]
-
enzyme form PO-FAT, pH 9.0, 37C
0.0036
-
palmitoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.0038
-
palmitoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.0041
-
palmitoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0043
-
palmitoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.0049
-
palmitoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.00034
-
stearoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 15 days after flowering
0.00054
-
stearoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00088
-
stearoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.0009
-
stearoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.00092
-
stearoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 30 days after flowering
0.0025
-
stearoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.003
-
stearoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.003
-
stearoyl-[acyl-carrier protein]
-
pH 9, 37C
0.0034
-
stearoyl-[acyl-carrier protein]
-
enzyme form PO-FAT, pH 9.0, 37C
0.005
-
stearoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.00563
-
stearoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.0068
-
stearoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.00954
-
stearoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.01
-
stearoyl-[acyl-carrier protein]
-
-
0.0067
-
undecanoyl-[acyl-carrier protein]
-
-
0.0166
-
lauroyl-[acyl-carrier protein]
-
-
additional information
-
additional information
-
Km is slightly influenced by substrate chain length and double bonds
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.004
-
myristoleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.22
-
myristoleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.008
-
myristoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.02
-
myristoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.25
-
oleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.67
-
oleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.98
-
oleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
1.23
-
oleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
1.94
-
oleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
2.94
-
oleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
6.08
-
oleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
7.99
-
oleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
11.4
-
oleoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.12
-
palmitoleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.15
-
palmitoleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.22
-
palmitoleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.45
-
palmitoleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.74
-
palmitoleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
1.62
-
palmitoleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
6.08
-
palmitoleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.03
-
palmitoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.04
-
palmitoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.41
-
palmitoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.44
-
palmitoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.46
-
palmitoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.5
-
palmitoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
2.4
-
palmitoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.15
-
stearoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.18
-
stearoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.38
-
stearoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.4
-
stearoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.56
-
stearoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.88
-
stearoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
0.91
-
stearoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
6.08
-
stearoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.9
-
(9Z)-hexadec-9-enoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
additional information
-
additional information
-
recombinant chimeric mutants FatAB and FatBA
-
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
80
-
oleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
14802
990
-
oleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
14802
12910
-
oleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
14802
19030
-
oleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
14802
30
-
palmitoleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
22673
250
-
palmitoleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
22673
1300
-
palmitoleoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
22673
4630
-
palmitoleoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
22673
30
-
palmitoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
22675
120
-
palmitoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
22675
760
-
palmitoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
22675
830
-
palmitoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
22675
30
-
stearoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
23240
90
-
stearoyl-[acyl-carrier protein]
D2E6T0, D5FFZ2
pH 8.0, 22C
23240
440
-
stearoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
23240
1030
-
stearoyl-[acyl-carrier protein]
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
23240
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0018
-
-
-
0.043
-
D2E6T0, D5FFZ2
palmitoyl-[acyl-carrier protein], pH 8.0, 22C
0.144
-
-
enzyme from leaf epidermis
0.18
-
-
substrate stearoyl-[acyl-carrier protein], pH 9, 37C
0.262
-
D2E6T0, D5FFZ2
palmitoleoyl-[acyl-carrier protein], pH 8.0, 22C
0.324
-
D2E6T0, D5FFZ2
stearoyl-[acyl-carrier protein], pH 8.0, 22C
0.437
-
D2E6T0, D5FFZ2
oleoyl-[acyl-carrier protein], pH 8.0, 22C
0.69
-
-
seed 65 days after flowering, enzyme form PO-FAT
0.744
-
D2E6T0, D5FFZ2
palmitoleoyl-[acyl-carrier protein], pH 8.0, 22C
0.778
-
D2E6T0, D5FFZ2
palmitoyl-[acyl-carrier protein], pH 8.0, 22C
1.45
-
D2E6T0, D5FFZ2
stearoyl-[acyl-carrier protein], pH 8.0, 22C
1.61
-
D2E6T0, D5FFZ2
oleoyl-[acyl-carrier protein], pH 8.0, 22C
11
-
-
isozyme AH1
15
-
-
recombinant enzyme in Escherichia coli, substrate lauroyl-CoA
192
-
-
purified enzyme form PO-FAT
1500
-
-
recombinant enzyme in Escherichia coli, substrate lauroyl-[acyl-carrier-protein]
additional information
-
-
-
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
9
-
medium-chain acyl-ACP thioesterase
8.5
-
-
assay at
8.5
-
-
enzyme from leaf epidermis
9
-
-
enzyme form PO-FAT
9.3
-
-
oleoyl-ACP thioesterase
9.8
-
-
isozymes AH1, AH2
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.8
9
-
medium-chain acyl-ACP thioesterase
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
-
room temperature, assay at
22
-
-
assay at room temperature
25
-
-
assay at
36
-
-
around 35-37C
37
-
-
assay at
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.2
-
D2E6T0, D5FFZ2
calculated
6.57
-
-
sequence analysis
6.7
-
A9XK92, A9XUG5
calculated from sequence
7
-
A9XK92, A9XUG5
calculated from sequence
7
-
-
chromatofocusing
7.1
-
D2E6T0, D5FFZ2
calculated
8.1
-
-
amino acid sequence calculation
8.2
-
-
enzyme form LC-FAT, chromatofocusing
9.7
-
-
enzyme form PO-FAT, chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
developing fruit
Manually annotated by BRENDA team
Elaeis sp.
-
-
Manually annotated by BRENDA team
Q2Q1N7, Q2Q1N8
-
Manually annotated by BRENDA team
D2E6T0, D5FFZ2
developing kernel; developing kernel
Manually annotated by BRENDA team
Elaeis sp.
-
high level expression
Manually annotated by BRENDA team
Q2Q1N7, Q2Q1N8
-
Manually annotated by BRENDA team
Populus tomentosa Carr
-
-
-
Manually annotated by BRENDA team
-
stearoyl-ACP thioestersae is predominantly expressed in epidermis
Manually annotated by BRENDA team
Elaeis sp.
-
15-18 weeks old tissue, high level expression, 8-12 weeks old tissue, low level expression
Manually annotated by BRENDA team
Q2Q1N7, Q2Q1N8
-
Manually annotated by BRENDA team
-
lowest level
Manually annotated by BRENDA team
Populus tomentosa Carr
-
-
-
Manually annotated by BRENDA team
-
developing, period from 45 to 93 days after flowering
Manually annotated by BRENDA team
-
highest enzyme activity during seed development 15 days after flowering
Manually annotated by BRENDA team
-
developing, fatty acid composition, overview
Manually annotated by BRENDA team
Q2Q1N7, Q2Q1N8
; CcFATB1 is seed specific
Manually annotated by BRENDA team
A9XK92, A9XUG5
;
Manually annotated by BRENDA team
-
highest level
Manually annotated by BRENDA team
Helianthus annuus RHA-274
-
-
-
Manually annotated by BRENDA team
Elaeis sp.
-
germinating, low level expression
Manually annotated by BRENDA team
-, Q9FT16
15-60 days after flowering
Manually annotated by BRENDA team
A1XAM4, -
expression of PtFATB is in a constitutive fashion
Manually annotated by BRENDA team
Populus tomentosa Carr
-
expression of PtFATB is in a constitutive fashion
-
Manually annotated by BRENDA team
additional information
Elaeis sp.
-
no expression in kernel tissues
Manually annotated by BRENDA team
additional information
-, Q9FT16
no detectable mRNA in root, leaf, and hypocotyl
Manually annotated by BRENDA team
additional information
-
tissue-specific expression pattern
Manually annotated by BRENDA team
additional information
Q2Q1N7, Q2Q1N8
CcFATB2 is expressed ubiquitously
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
putative chloroplast transit peptide at the N-terminal
Manually annotated by BRENDA team
-
nuclear gene encoded, targeted to the plastids, soluble
Manually annotated by BRENDA team
additional information
Elaeis sp.
-
the gene contains a putative transit peptide sequence
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
28000
-
-
medium-chain acyl-ACP thioesterase, gel filtration
36800
-
A9XK92, A9XUG5
recombinant histidine-tagged FatB, gel filtration
37250
-
-
calculation from sequence of cDNA
37520
-
A9XK92, A9XUG5
recombinant histidine-tagged FatA, gel filtration
41960
-
-
precusor form, encoded by the pNL2 gene, calculation from sequence of cDNA
41980
-
-
precusor form, encoded by the pNL3 gene, calculation from sequence of cDNA
42200
-
A9XK92, A9XUG5
FatA open reading frames, calculated from sequence
45000
-
-
gel filtration
46500
-
A9XK92, A9XUG5
FatB open reading frames, calculated from sequence
48080
-
-
sequence analysis
51000
-
-
isozymes AH1, AH2, TSKgel G3000SW gel filtration
53000
-
-
oleoyl-ACP thioesterase, gel filtration
57000
-
-
isozyme AH3, TSKgel G3000SW gel filtration
59000
-
-
isozyme AH1, gel filtration
70000
80000
-
gel filtration
72000
-
-
gel filtration
78000
-
-
gel filtration
84000
-
-
enzyme from leaf epidermis, gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Elaeis sp.
-
x * 66000, recombinant glutathione S-transferase fusion protein, SDS-PAGE
?
-
x * 41200, about, amino acid sequence calculation, x * 36900, recombinant His-tagged enzyme without signal peptide, SDS-PAGE
?
A9XK92, A9XUG5
x * 42200, FatA open reading frames, calculated from sequence; x * 46500, FatA open reading frames, calculated from sequence
?
-
x * 48080, calculated from sequence
?
D2E6T0, D5FFZ2
x * 45900, calculated; x * 46200, calculated
?
Helianthus annuus RHA-274
-
x * 41200, about, amino acid sequence calculation, x * 36900, recombinant His-tagged enzyme without signal peptide, SDS-PAGE
-
dimer
-
2 * 38000, SDS-PAGE
dimer
-
2 * 40000, enzyme from leaf epidermis, SDS-PAGE
dimer
-
2 * 35000, SDS-PAGE
homodimer
-
2 * 40000, Western blot analysis
monomer
-
1 * 42500-44000, SDS-PAGE
additional information
Q42562
sequence-predicted three-dimensional structure, comprises two helix/4-stranded sheet domains, the N-terminal domain contains residues that affect specificity, and the C-terminal domain contains the catalytic residues, modelling, PDB code 1XXY, structure comparisons to other plant thioesterases, overview
additional information
-
secondary structure and predicted three-dimensional structure, modeling and analysis
additional information
Helianthus annuus RHA-274
-
secondary structure and predicted three-dimensional structure, modeling and analysis
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
proteolytic modification
-
the 40 kDa protein is cleaved to a 34 kDa protein within 3 h. This degradation does not change activity, it is unknown whether this degradation is of physiological significance
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35
-
-
activity relatively stable at 35C
50
-
-
20 min stable
60
-
-
15 min, enzyme form LC-FAT is mostly inactivated, while enzyme form PO-FAT is activated by 15-20%
60
-
-
60% activity lost after 15 min at 60C, 75% activity lost after 30 min at 60C
70
-
-
15 min, enzyme form PO-FAT is heat-stable, after 45 min the enzyme retains 30% of the original activity
80
-
-
15 min, 30% remaining activity
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
glycerol, 25%, is required for stability during purification for the enzyme form PO-FAT
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C, enzyme form PO-FAT, ammonium sulfate pellet, stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
from leaf epidermis
-
native enzyme to homogeneity from developing seeds by hydroxyapatite, ion exchange, and [acyl carrier protein] affinity chromatographies, and preparative native PAGE
-
partial purification of the isozymes and the recombinant chimeric mutants
-
isozyme AH1
-
two thioesterase activities: medium-chain acyl-ACP thioesterase and oleoyl-ACP thioesterase
-
enzyme form PO-FAT, 499fold
-
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography
-
immobilized metal ion affinity chromatography (Ni2+); immobilized metal ion affinity chromatography (Ni2+)
D2E6T0, D5FFZ2
ammonium sulfate precipitation, ion exchange chromatography (DEAE), [acyl-carrier protein]-affinity chromatography
-
var. Fuerte
-
to homogeneity in a single step by gel filtration; to homogeneity in a single step by gel filtration
A9XK92, A9XUG5
partial purification
-
Uc FatB1 and mutants
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
overexpression in seeds of Arabidopsis thaliana under control of a seed-specific promotor via Agrobacterium tumefaciens infection resulting in accumulation of high amounts of palmitate, anti-sense expression of the cDNA in seeds under control of the S35 promotor from cauliflower mosaic virus leading to reduced enzyme activity in flowers and seeds but not in leaves, tissue-specific expression pattern
-
overexpression in transgenic wheat
-
DNA sequence determination and analysis
-, Q9FT16
expression in Escherichia coli
-
expressed in Nicotiana tabacum
-
expressed in Phaeodactylum tricornutum
Q39473
expression in Escherichia coli
-
overexpression of wild-type isozymes and chimeric mutants in Escherichia coli Bl21(DE3), the chimeric mutants are constructed by combination of the N-terminus of FatA with the C-terminus of FatB resulting in FatAB, or vice versa resulting in FatBA
-
overexpression of CcFATB1 in developing seeds of Arabidopsis partially redirects fatty acid synthesis toward medium-chain FA (12:0, 14:0, and 16:0). Overexpression of CcFATB1 cDNA complemented the low-palmitate (16:0) but not the low-stearate (18:0) phenotype in leaves and seeds of an Arabidopsis fatb-2 mutant; overexpression of CcFATB2 in developing seeds of Arabidopsis complements the low-palmitate (16:0) and the low-stearate (18:0) phenotype in leaves and seeds of an Arabidopsis fatb-2 mutant
Q2Q1N7, Q2Q1N8
expression in Escherichia coli
A0PGT6
DNA and amino acid sequence determination and analysis, construction of a cDNA library from kernel DNA
Q9XEL9
single gene, partial DNA sequence determination and DNA sequence analysis, expression in Escherichia coli BL21 (DE3) as glutathione S-transferase fusion protein
Elaeis sp.
-
expression of FatB as soluble enzyme in diverse Escherichia coli strains and in vitro transcription and translation lead to 2 different polypeptides by alternative initiation, a full-length 46 kDa form and a truncated 35 kDa form
Q9SQI3
gene fatA1, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression as soluble His-tagged enzyme in Escherichia coli strain BL21 and DH5alpha, lipid analysis in recombinant Escherichia coli cells
-
coding region (including the chloroplast targeting peptide) of FATB1 cDNA amplified, then ligated into the 2S::GUS plasmid vector, using XbaI/SacI to place the gusA gene behind the seed-specific promoter. The 2S::JcFATB1 construct transferred into Agrobacterium tumefaciens GV3101, and Arabidopsis plants for overexpression
-
expressed in Escherichia coli K27fadD88
-
a His-tagged version expressed in Escherichia coli XL-1 blue; a His-tagged version expressed in Escherichia coli XL-1 blue
D2E6T0, D5FFZ2
expression in Escherichia coli. The ectopically expressed MlFatB gene causes alteration in the fatty acid profile of Escherichia coli cells
A0PGT7, -
PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatA overexpressed in Escherichia coli XL1-Blue; PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatB overexpressed in Escherichia coli XL1-Blue
A9XK92, A9XUG5
expressed in Escherichia coli MG1655
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expressed in Phaeodactylum tricornutum
Q41634
expression of medium-chain acyl-acyl carrier protein thioesterase in Escherichia coli
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expression of UcFatB1 and mutants in Escherichia coli
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functional expression in Escherichia coli, diverse recombinant mutant strains
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EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
is most strongly expressed in seeds, in which its expression peaks in late developmental stages
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transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation; transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation
A9XK92, A9XUG5
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
K216A
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
K216E
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
R143A/K144A
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
R143E/K144E
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
R212A/R213A
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
R212E/R213E
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
K216A
Helianthus annuus RHA-274
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
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R143A/K144A
Helianthus annuus RHA-274
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
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R143E/K144E
Helianthus annuus RHA-274
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
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R212A/R213A
Helianthus annuus RHA-274
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
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R212E/R213E
Helianthus annuus RHA-274
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site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
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C320A
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no activity
C320S
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slightly reduced activity
H140A
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reduced activity
H140R
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reduced activity
H285A
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no activity
H285R
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reduced activity
H345A
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reduced activity
H345R
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reduced activity
additional information
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metabolic and morphological effects of transgenic expression of the enzyme together with the glycerol-3-phosphate acyltransferase from Pisum sativum in wheat plants
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
agriculture
A0PGT6
the new MbFatB gene isolated from Madhuca butyracea can be used in future for transgenic development of oil-seed Brassica, a widely cultivated crop that expresses predominantly oleoyl-ACP thioesterase (FatA) in its seed tissue and has high amount of unwanted erucic acid in edible oil in order to alter the fatty acid profile in a desirable way
industry
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FATB1 can function as a saturated acyl-ACP TE and may potentially modify the seed oil to increase its levels of palmitate
industry
A9XK92, A9XUG5
contribution of FatA to the synthesis of castor oil; contribution of FatB to the synthesis of castor oil