Information on EC 3.1.2.14 - oleoyl-[acyl-carrier-protein] hydrolase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.1.2.14
-
RECOMMENDED NAME
GeneOntology No.
oleoyl-[acyl-carrier-protein] hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an oleoyl-[acyl-carrier protein] + H2O = an [acyl-carrier protein] + oleate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of thioester
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acyl-ACP thioesterase pathway
-
-
bacterial bioluminescence
-
-
cis-vaccenate biosynthesis
-
-
mycobacterial sulfolipid biosynthesis
-
-
oleate biosynthesis I (plants)
-
-
palmitate biosynthesis I (animals and fungi)
-
-
palmitate biosynthesis II (bacteria and plants)
-
-
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)
-
-
palmitoleate biosynthesis II (plants and bacteria)
-
-
petroselinate biosynthesis
-
-
stearate biosynthesis II (bacteria and plants)
-
-
arachidonate biosynthesis
-
-
cis-vaccenate biosynthesis
-
-
palmitate biosynthesis
-
-
Fatty acid biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
oleoyl-[acyl-carrier protein] hydrolase
Acts on acyl-carrier-protein thioesters of fatty acids from C12 to C18, but the derivative of oleic acid is hydrolysed much more rapidly than any other compound tested.
CAS REGISTRY NUMBER
COMMENTARY hide
105669-84-9
-
68009-83-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Automatic Mining of ENzyme DAta
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-
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Automatic Mining of ENzyme DAta
Br FatA1
-
-
Manually annotated by BRENDA team
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-
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Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Manually annotated by BRENDA team
Coriandrum sativum Cs FatA1
Cs FatA1
-
-
Manually annotated by BRENDA team
isozymes AH1, AH2, AH3
-
-
Manually annotated by BRENDA team
var. mesostemon
SwissProt
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
SwissProt
Manually annotated by BRENDA team
Elaeis sp.
oil palm
-
-
Manually annotated by BRENDA team
strain RHA-274, gene fatA1
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-
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
var. Fuerte, avocado
-
-
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
Carr
SwissProt
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Manually annotated by BRENDA team
Um FatB1
-
-
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
-
the main change in the Escherichia coli fatty acid composition when CsFatA is expressed is the reduction in unsaturated fatty acids, mainly cis-vaccenic acid (18:1 omega7). This reduction is compensated for by an increase of palmitoleic acid (16:1 omega7); the main change in the Escherichia coli fatty acid composition when CsFatB is expressed is a decrease in saturated fatty acids and in particular, that of palmitic acid (16:0) that is compensated for with an increase in stearic acid (18.0)
physiological function
-
deduced amino acid sequence displays high homology to choroplastic palmitoyl-acyl carrier protein thioesterases from other plants
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
decanoyl-[acyl-carrier protein] + H2O
decanoic acid + acyl-carrier protein
show the reaction diagram
dodecenoyl-[acyl-carrier protein] + H2O
dodecenoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-CoA + H2O
lauric acid + CoA
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
myristoleoyl-[acyl-carrier protein] + H2O
myristoleic acid + acyl-carrier protein
show the reaction diagram
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
oleoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
endpoint of de novo fatty acid synthesis in plastids
-
-
-
oleoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + oleic acid
show the reaction diagram
oleoyl-[acyl-carrier protein] + H2O
oleate + acyl-carrier protein
show the reaction diagram
-
substrates are long chain acyl-acyl carrier proteins
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
oleoyl-[acyl-carrier-protein] + H2O
oleic acid + acyl-carrier-protein
show the reaction diagram
palmitoleoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + palmitoleic acid
show the reaction diagram
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
show the reaction diagram
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleic acid + acyl-carrier protein
show the reaction diagram
palmitoleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + palmitoleate
show the reaction diagram
palmitoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + palmitic acid
show the reaction diagram
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
palmitoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + palmitate
show the reaction diagram
-
-
-
-
?
palmitoyl-[acyl-carrier-protein] + H2O
palmitic acid + acyl-carrier-protein
show the reaction diagram
12% of the activity with stearoyl-[acyl-carrier-protein]
-
-
?
stearoyl-ACP + H2O
stearate + acyl-carrier protein
show the reaction diagram
-
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
synthesis of fatty acids for wax biosynthesis
-
-
-
stearoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + stearic acid
show the reaction diagram
stearoyl-[acyl-carrier protein] + H2O
stearate + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
stearoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + stearate
show the reaction diagram
stearoyl-[acyl-carrier-protein] + H2O
stearic acid + acyl-carrier-protein
show the reaction diagram
-
-
-
?
undecanoyl-[acyl-carrier protein] + H2O
undecanoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
oleoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
endpoint of de novo fatty acid synthesis in plastids
-
-
-
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
stearoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
synthesis of fatty acids for wax biosynthesis
-
-
-
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(NH4)2SO4
5,5'-dithiobis(2-nitrobenzoate)
diethyldicarbonate
-
-
iodoacetamide
-
-
N-ethylmaleimide
-
-
oleoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
palmitoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
stearoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0006
(9Z)-hexadec-9-enoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.0023 - 0.004
decanoyl-[acyl-carrier protein]
0.0049
dodecanoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.0146 - 0.0166
lauroyl-[acyl-carrier protein]
0.0029 - 0.0139
myristoleoyl-[acyl-carrier protein]
0.0028 - 0.0089
myristoyl-[acyl-carrier protein]
0.0024 - 0.029
octanoyl-[acyl-carrier protein]
0.00008 - 3
oleoyl-[acyl-carrier protein]
0.0006 - 0.0212
oleoyl-[acyl-carrier-protein]
0.0001 - 0.00551
palmitoleoyl-[acyl-carrier protein]
0.00028 - 0.0066
palmitoyl-[acyl-carrier protein]
0.00034 - 0.01
stearoyl-[acyl-carrier protein]
0.0067
undecanoyl-[acyl-carrier protein]
-
-
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
(9Z)-hexadec-9-enoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.004 - 0.22
myristoleoyl-[acyl-carrier protein]
0.008 - 0.02
myristoyl-[acyl-carrier protein]
0.25 - 11.4
oleoyl-[acyl-carrier protein]
0.032 - 35.6
oleoyl-[acyl-carrier-protein]
0.0016 - 8
palmitoleoyl-[acyl-carrier protein]
0.0005 - 4.4
palmitoyl-[acyl-carrier protein]
0.0002 - 6.08
stearoyl-[acyl-carrier protein]
additional information
additional information
Arabidopsis thaliana
-
recombinant chimeric mutants FatAB and FatBA
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
80 - 19030
oleoyl-[acyl-carrier protein]
1933
50 - 39300
oleoyl-[acyl-carrier-protein]
67097
28 - 48000
palmitoleoyl-[acyl-carrier protein]
3103
3 - 2800
palmitoyl-[acyl-carrier protein]
1598
2 - 1600
stearoyl-[acyl-carrier protein]
1770
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.043
palmitoyl-[acyl-carrier protein], pH 8.0, 22C
0.144
-
enzyme from leaf epidermis
0.18
-
substrate stearoyl-[acyl-carrier protein], pH 9, 37C
0.262
palmitoleoyl-[acyl-carrier protein], pH 8.0, 22C
0.324
stearoyl-[acyl-carrier protein], pH 8.0, 22C
0.437
oleoyl-[acyl-carrier protein], pH 8.0, 22C
0.69
-
seed 65 days after flowering, enzyme form PO-FAT
0.744
palmitoleoyl-[acyl-carrier protein], pH 8.0, 22C
0.778
palmitoyl-[acyl-carrier protein], pH 8.0, 22C
1.45
stearoyl-[acyl-carrier protein], pH 8.0, 22C
1.61
oleoyl-[acyl-carrier protein], pH 8.0, 22C
11
-
isozyme AH1
15
-
recombinant enzyme in Escherichia coli, substrate lauroyl-CoA
192
-
purified enzyme form PO-FAT
1500
-
recombinant enzyme in Escherichia coli, substrate lauroyl-[acyl-carrier-protein]
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
medium-chain acyl-ACP thioesterase
9.3
-
oleoyl-ACP thioesterase
9.8
-
isozymes AH1, AH2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8 - 9
-
medium-chain acyl-ACP thioesterase
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36
-
around 35-37C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.57
-
sequence analysis
6.7
calculated from sequence
8.1
-
amino acid sequence calculation
8.2
-
enzyme form LC-FAT, chromatofocusing
9.7
-
enzyme form PO-FAT, chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
developing fruit
Manually annotated by BRENDA team
-
stearoyl-ACP thioestersae is predominantly expressed in epidermis
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
Elaeis sp.
-
the gene contains a putative transit peptide sequence
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
-
medium-chain acyl-ACP thioesterase, gel filtration
33400
-
calculated from cDNA
34800
-
calculated from cDNA
36800
recombinant histidine-tagged FatB, gel filtration
37000
-
SDS-PAGE
37250
-
calculation from sequence of cDNA
37520
recombinant histidine-tagged FatA, gel filtration
40500
-
SDS-PAGE
41960
-
precusor form, encoded by the pNL2 gene, calculation from sequence of cDNA
41980
-
precusor form, encoded by the pNL3 gene, calculation from sequence of cDNA
42200
FatA open reading frames, calculated from sequence
45000
-
gel filtration
46500
FatB open reading frames, calculated from sequence
48080
-
sequence analysis
51000
-
isozymes AH1, AH2, TSKgel G3000SW gel filtration
53000
-
oleoyl-ACP thioesterase, gel filtration
57000
-
isozyme AH3, TSKgel G3000SW gel filtration
59000
-
isozyme AH1, gel filtration
61000
-
SDS-PAGE, recombinant protein
70000 - 80000
-
gel filtration
78000
-
gel filtration
84000
-
enzyme from leaf epidermis, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
-
2 * 40000, Western blot analysis
monomer
-
1 * 42500-44000, SDS-PAGE
additional information