Information on EC 3.1.2.14 - oleoyl-[acyl-carrier-protein] hydrolase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.1.2.14
-
RECOMMENDED NAME
GeneOntology No.
oleoyl-[acyl-carrier-protein] hydrolase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an oleoyl-[acyl-carrier protein] + H2O = an [acyl-carrier protein] + oleate
show the reaction diagram
-
-
-
-
an oleoyl-[acyl-carrier protein] + H2O = an [acyl-carrier protein] + oleate
show the reaction diagram
catalytic residues are Asn227, Cys264 and His229, forming a papain-like catalytic triad
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of thioester
-
-
-
-
hydrolysis of thioester
-
-
hydrolysis of thioester
-
-
hydrolysis of thioester
Elaeis sp.
-
-
hydrolysis of thioester
-
hydrolysis of thioester
-
-
hydrolysis of thioester
-
-
hydrolysis of thioester
-
hydrolysis of thioester
-
-
hydrolysis of thioester
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
acyl-ACP thioesterase pathway
-
-
cis-vaccenate biosynthesis
-
-
oleate biosynthesis I (plants)
-
-
palmitate biosynthesis I (animals and fungi)
-
-
palmitate biosynthesis II (bacteria and plants)
-
-
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)
-
-
palmitoleate biosynthesis II (plants and bacteria)
-
-
petroselinate biosynthesis
-
-
stearate biosynthesis II (bacteria and plants)
-
-
arachidonate biosynthesis
-
-
cis-vaccenate biosynthesis
-
-
palmitate biosynthesis
-
-
Fatty acid biosynthesis
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
oleoyl-[acyl-carrier protein] hydrolase
Acts on acyl-carrier-protein thioesters of fatty acids from C12 to C18, but the derivative of oleic acid is hydrolysed much more rapidly than any other compound tested.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
12:0-ACP thioesterase
-
-
-
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
Elaeis sp.
-
-
12:0-ACP thioesterase
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
-
12:0-ACP thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
-
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
Elaeis sp.
-
-
12:0-acyl-carrier protein thioesterase
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
12:0-acyl-carrier protein thioesterase
-
-
14:0-ACP thioesterase
-
-
-
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
Elaeis sp.
-
-
14:0-ACP thioesterase
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
-
14:0-ACP thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
-
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
Elaeis sp.
-
-
14:0-acyl-carrier protein thioesterase
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
14:0-acyl-carrier protein thioesterase
-
-
16:0-ACP thioesterase
-
-
-
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
Elaeis sp.
-
-
16:0-ACP thioesterase
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
-
16:0-ACP thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
-
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
Elaeis sp.
-
-
16:0-acyl-carrier protein thioesterase
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
16:0-acyl-carrier protein thioesterase
-
-
18:0-ACP thioesterase
-
-
-
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
Elaeis sp.
-
-
18:0-ACP thioesterase
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
-
18:0-ACP thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
-
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
Elaeis sp.
-
-
18:0-acyl-carrier protein thioesterase
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
18:0-acyl-carrier protein thioesterase
-
-
acyl-ACP TE
-
acyl-ACP TE
-
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
-
acyl-ACP thioesterase
myristic acid biased
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
Helianthus annuus RHA-274
-
-
-
acyl-ACP thioesterase
-
acyl-ACP thioesterase
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
-
-
acyl-ACP thioesterase
lauric acid biased
acyl-ACP thioesterase B
-
-
acyl-ACP thioesterase FatA
-
-
acyl-ACP thioesterase FatA type
-
acyl-ACP thioesterase FatB type
-
acyl-ACP-hydrolase
-
-
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
Elaeis sp.
-
-
acyl-ACP-hydrolase
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-hydrolase
-
-
acyl-ACP-thioesterase
-
-
acyl-acyl carrier protein (ACP) thioesterase
-
acyl-acyl carrier protein hydrolase
-
-
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
Elaeis sp.
-
-
acyl-acyl carrier protein hydrolase
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein hydrolase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
Helianthus annuus RHA-274
-
-
-
acyl-acyl carrier protein thioesterase
-
acyl-acyl carrier protein thioesterase
-
acyl-acyl carrier protein thioesterase
Populus tomentosa Carr
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase
-
-
acyl-acyl carrier protein thioesterase B1
-
-
acyl-acyl carrier protein-thioesterase
-
-
acyl-acyl-carrier protewin thioesterase
-
-
Acyl-[acyl-carrier protein] hydrolase
-
-
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
Elaeis sp.
-
-
acyl-[acyl-carrier-protein] hydrolase
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acyl-[acyl-carrier-protein] hydrolase
-
-
acylACP thioesterase
-
acylacyl carrier protein (ACP) thioesterase
-
AH
-
-
-
-
AH
Elaeis sp.
-
-
Ah FatA
-
-
BnFatB(2)
-
-
BTE
-
-
-
-
BTE
Elaeis sp.
-
-
C12:0 ACP TE
-
-
-
-
C12:0 ACP TE
-
C12:0 ACP TE
-
-
C12:0 ACP TE
-
-
C12:0 ACP TE
-
-
C12:0 ACP TE
Elaeis sp.
-
-
C12:0 ACP TE
-
-
C12:0 ACP TE
-
-
C12:0 ACP TE
-
-
C18:1-ACP thioesterase
-
-
-
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
Elaeis sp.
-
-
C18:1-ACP thioesterase
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
-
C18:1-ACP thioesterase
-
-
Deubiquitinating enzyme 14
-
-
-
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
Elaeis sp.
-
-
Deubiquitinating enzyme 14
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
-
Deubiquitinating enzyme 14
-
-
FatA-type thioesterase
-
-
FatA-type thioesterase
Helianthus annuus RHA-274
-
-
-
FatA1 thioesterase
-
-
FatA1 thioesterase
Helianthus annuus RHA-274
-
-
-
fatty acyl-ACP thioesterase
-
-
fatty acyl-ACP thioesterase
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
Elaeis sp.
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
hydrolase, oleoyl-[acyl carrier protein]
-
-
LC-FAT
-
oleoyl-[acyl carrier protein] specific
oleoyl-ACP thioesterase
-
-
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
oleoyl-ACP thioesterase
Elaeis sp.
-
-
oleoyl-ACP thioesterase
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-ACP thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
-
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
Elaeis sp.
-
-
oleoyl-acyl carrier protein thioesterase
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
oleoyl-acyl carrier protein thioesterase
-
-
OPSpTE
-
codon optimised SpTE containing replaced native codons from Escherichia coli MG1655- and W3110-favored codons
OTE
-
-
-
-
OTE
Elaeis sp.
-
-
palmitoyl-ACP thioesterase
-
palmitoyl-acyl carrier protein thioesterase
Elaeis sp.
-
-
palmitoyl-acyl carrier protein thioesterase
-
PATE
-
-
-
-
PATE
Elaeis sp.
-
-
PO-FAT
-
palmitoyl-/oleoyl-[acyl carrier protein] specific
PtFATB
Populus tomentosa Carr
-
-
saturated acyl ACP thioesterase type B
-
seed-specific acyl-acyl carrier protein thioesterase
-
STE
-
-
-
-
STE
Elaeis sp.
-
-
stearoyl/oleoyl specific fatty acyl-acyl carrier protein thioesterase
-
stearoyl/oleoyl-specific Fat
-
Ubiquitin thiolesterase 14
-
-
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
Elaeis sp.
-
-
Ubiquitin thiolesterase 14
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin thiolesterase 14
-
-
Ubiquitin-specific processing protease 14
-
-
-
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
Elaeis sp.
-
-
Ubiquitin-specific processing protease 14
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
-
Ubiquitin-specific processing protease 14
-
-
CAS REGISTRY NUMBER
COMMENTARY
105669-84-9
-
68009-83-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
At FatA1 and At FatB1
-
-
Manually annotated by BRENDA team
ecotype Col-2
-
-
Manually annotated by BRENDA team
gene fatB
Uniprot
Manually annotated by BRENDA team
isozyme FatA and FatB
-
-
Manually annotated by BRENDA team
cv. Pusa Bold
SwissProt
Manually annotated by BRENDA team
Bn FatA1
-
-
Manually annotated by BRENDA team
cv. Quantum
-
-
Manually annotated by BRENDA team
Br FatA1
-
-
Manually annotated by BRENDA team
safflower, Ct FatA1 and Ct FatA2
-
-
Manually annotated by BRENDA team
Cs FatA1
-
-
Manually annotated by BRENDA team
isozyme FatA
-
-
Manually annotated by BRENDA team
Coriandrum sativum Cs FatA1
Cs FatA1
-
-
Manually annotated by BRENDA team
isozymes AH1, AH2, AH3
-
-
Manually annotated by BRENDA team
var. mesostemon
SwissProt
Manually annotated by BRENDA team
Ch FatA1 and Ch FatB1 and Ch FatB2
-
-
Manually annotated by BRENDA team
Cl FatB1
-
-
Manually annotated by BRENDA team
fragment of precursor
SwissProt
Manually annotated by BRENDA team
i.e. Brassica butyracea, 2 enzyme forms: a palmitoyl-/oleoyl-[acyl carrier protein] specific and a oleoyl-[acyl carrier protein] specific one, i.e. PO-FAT and LC-FAT, respectively
-
-
Manually annotated by BRENDA team
Elaeis sp.
oil palm
-
-
Manually annotated by BRENDA team
Gm FatA1 and Gm FatA2
-
-
Manually annotated by BRENDA team
cv. Paymaster HS26, gene FatB
SwissProt
Manually annotated by BRENDA team
line RHA-274 with normal fatty acid content and high-palmitic seed oil line CAS-5, 2 isozymes: FatA type and FatB type
-
-
Manually annotated by BRENDA team
strain RHA-274, gene fatA1
-
-
Manually annotated by BRENDA team
Helianthus annuus RHA-274
strain RHA-274, gene fatA1
-
-
Manually annotated by BRENDA team
fragment of precursor
UniProt
Manually annotated by BRENDA team
var. Fuerte, avocado
-
-
Manually annotated by BRENDA team
Populus tomentosa Carr
Carr
SwissProt
Manually annotated by BRENDA team
Um FatB1
-
-
Manually annotated by BRENDA team
bay, UcFatB1
-
-
Manually annotated by BRENDA team
Uc FatB1 and Uc FatB2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
overexpression of ClFATB1 in transgenic tobaccos upregulates thioesterase activities of crude proteins against 16:0-ACP and 18:0-ACP by 20.3 and 5.7%, respectively, and results in an increase in the contents of palmitic and stearic acids by 15.4 and 10.5%, respectively. Ectopic expression of this gene decreases the substrate specificities of crude proteins to unsaturated 18:1-ACP by 12.7% in transgenic tobacco and lowers the contents of oleic, linoleic, and linolenic acids in transgenic leaves
malfunction
-
expressed in Escherichia coli the transformants disply an increased decanoic acid and lauric acid contents
malfunction
-
heterologous expression of AhFatA in Escherichia coli affect bacterial growth and change the fatty acid profiles of the membrane lipid, resulting in directed accumulation towards palmitoleic acid and oleic acid
malfunction
-
simultaneous over-expression of a cDNA encoding a native FatB thioesterase and down-regulation of SAD gene expression, leads to plants containing up to 46% saturated fatty acids
malfunction
-
when overexpressed in transformant tobacco plants CpFATB affects the fatty acid composition of transformants and is able to increase drought tolerance in these plants
malfunction
-
overexpression of ClFATB1 in transgenic tobaccos upregulates thioesterase activities of crude proteins against 16:0-ACP and 18:0-ACP by 20.3 and 5.7%, respectively, and results in an increase in the contents of palmitic and stearic acids by 15.4 and 10.5%, respectively. Ectopic expression of this gene decreases the substrate specificities of crude proteins to unsaturated 18:1-ACP by 12.7% in transgenic tobacco and lowers the contents of oleic, linoleic, and linolenic acids in transgenic leaves
malfunction
-
null, wild type and high-efficiency alleles are transiently expressed in tobacco leaves to check their effect on lipid biosynthesis. Expression of active FatA thioesterases alters the composition of leaf triacylglycerols but does not alter total lipid content. The expression of the wild type and the high-efficiency alleles in Arabidopsis thaliana transgenic seeds results in a strong reduction in oil content and an increase in total saturated fatty acid content
metabolism
-
the main change in the Escherichia coli fatty acid composition when CsFatA is expressed is the reduction in unsaturated fatty acids, mainly cis-vaccenic acid (18:1 omega7). This reduction is compensated for by an increase of palmitoleic acid (16:1 omega7); the main change in the Escherichia coli fatty acid composition when CsFatB is expressed is a decrease in saturated fatty acids and in particular, that of palmitic acid (16:0) that is compensated for with an increase in stearic acid (18.0)
physiological function
-
deduced amino acid sequence displays high homology to choroplastic palmitoyl-acyl carrier protein thioesterases from other plants
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
role in controlling the acyl flux through the eucaryotic and procaryotic pathway in plants
-
-
-
decanoyl-[acyl-carrier protein] + H2O
decanoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
decanoyl-[acyl-carrier protein] + H2O
decanoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
-
dodecenoyl-[acyl-carrier protein] + H2O
dodecenoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-CoA + H2O
lauric acid + CoA
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
low activity
-
?
myristoleoyl-[acyl-carrier protein] + H2O
myristoleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
myristoleoyl-[acyl-carrier protein] + H2O
myristoleic acid + acyl-carrier protein
show the reaction diagram
-
low activity
-
?
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
-
-
-
-
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
-
-
?
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
-
low activity
-
?
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
-
activity of the TE 3-2 clone expressed in E. coli
-
?
myristoyl-[acyl-carrier protein] + H2O
myristic acid + acyl-carrier protein
show the reaction diagram
-
activity of the medium-chain acyl-ACP thioesterase
-
?
oleoyl-ACP-ACP + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
activity of the TE 3-2 clone expressed in E. coli
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
activity of the oleoyl-ACP thioesterase
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
activity of the clones pCGN3270 and pCGN3271 expressed in E. coli
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
most reactive substrate
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of FatA type isozyme, low activity with FatB type isozyme
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of recombinant chimeric mutant FatAB
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of the oleoyl-[acyl carrier protein] specific enzyme form LC-FAT
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
substrates are long chain acyl-acyl carrier proteins, role in fatty acid biosynthesis
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
high efficiency of FatB
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
maximum catalytic efficiency of FatA
-
?
oleoyl-[acyl-carrier protein] + H2O
oleate + acyl-carrier protein
show the reaction diagram
-
substrates are long chain acyl-acyl carrier proteins
-
?
oleoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
endpoint of de novo fatty acid synthesis in plastids
-
-
-
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
-
preferred substrate
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
-
preferred substrate
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
preferred substrate
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
-
preferred substrate of wild-type and recombinant enzyme
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
Helianthus annuus RHA-274
-
preferred substrate, preferred substrate of wild-type and recombinant enzyme
-
?
oleoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + oleic acid
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + oleic acid
show the reaction diagram
-
-
?
oleoyl-[acyl-carrier-protein] + H2O
oleic acid + acyl-carrier-protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier-protein] + H2O
oleic acid + acyl-carrier-protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier-protein] + H2O
oleic acid + acyl-carrier-protein
show the reaction diagram
88.2% of the activity with stearoyl-[acyl-carrier-protein]
-
?
palmitoleoyl-ACP-ACP + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + palmitoleate
show the reaction diagram
Helianthus annuus, Helianthus annuus RHA-274
-
-
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
show the reaction diagram
-
-
-
-
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
show the reaction diagram
-
-
-
-
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
show the reaction diagram
high catalytic efficiency of FatA
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
palmitoleate + acyl-carrier protein
show the reaction diagram
high efficiency of FatB
-
?
palmitoleoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + palmitoleic acid
show the reaction diagram
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
best substrate
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
activity of the TE 3-2 clone expressed in E. coli
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
10% of the activity with oleoyl-[acyl-carrier-protein]
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
activity of the clone pCGN3271 expressed in E. coli
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of FatB type isozyme
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of recombinant chimeric mutant FatBA, isozyme FatB prefers [acyl-carrier-protein]-substrate of spinach before the Escherichia coli derived
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of the palmitoyl-/oleoyl-[acyl carrier protein] specific enzyme form PO-FAT
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
enzyme plays an important role in the control of seed-oil composition, determination of fatty acid chain growth
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
best substrate
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
best substrate, role in fatty acid biosynthesis in flowers and seeds, overview about the fatty acid composition of the plants
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
FATB1 is a palmitoyl-acyl-acyl carrier protein thioesterase. Seed-specific expression of FATB1 in Arabidopsis leads to a 3-4fold increase in the proportion of accumulated palmitate (up to 40.9 mol%) and moderate increases in the proportions of other saturated fatty acids, with associated reductions in the proportions of unsaturated fatty acids. Residues H317 and C352 are essential for catalytic activity
-
?
palmitoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + palmitate
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + palmitic acid
show the reaction diagram
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + palmitic acid
show the reaction diagram
-
relative specific activity to oleoyl-[acyl-carrier protein]: 1.8%
-
?
palmitoyl-[acyl-carrier-protein] + H2O
palmitic acid + acyl-carrier-protein
show the reaction diagram
12% of the activity with stearoyl-[acyl-carrier-protein]
-
?
stearoyl-ACP + H2O
stearate + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
activity of the TE 3-2 clone expressed in E. coli
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
10% of the activity with oleoyl-[acyl-carrier-protein]
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
activity of the clone pCGN3271 expressed in E. coli
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
highly specific for, leaf epidermis enzyme
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
-
preferred substrate of FatB type isozyme, low activity with FatA type isozyme
-
?
stearoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
synthesis of fatty acids for wax biosynthesis
-
-
-
stearoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + stearate
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + stearate
show the reaction diagram
-
low activity
-
?
stearoyl-[acyl-carrier protein] + H2O
stearate + acyl-carrier protein
show the reaction diagram
-
-
?
stearoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + stearic acid
show the reaction diagram
-
-
?
stearoyl-[acyl-carrier protein] + H2O
acyl-carrier protein + stearic acid
show the reaction diagram
-
relative specific activity to oleoyl-[acyl-carrier protein]: 35%
-
?
stearoyl-[acyl-carrier-protein] + H2O
stearic acid + acyl-carrier-protein
show the reaction diagram
-
-
?
undecanoyl-[acyl-carrier protein] + H2O
undecanoic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
lauroyl-[acyl-carrier protein] + H2O
lauric acid + acyl-carrier protein
show the reaction diagram
-
activity of the medium-chain acyl-ACP thioesterase
-
?
additional information
?
-
substrate specificity
-
-
?
additional information
?
-
-
Fat A thioesterases are specific for unsaturated substrates e.d. 18:1-ACP, FatB thioesterases form two groups are either specific for saturated acyl chain of C-8 to C-14, i.e. medium-chain length, or specific for C-14 to C-18 acyl chain length
-
-
-
additional information
?
-
-
the reaction rate increases with increasing chain length of acyl from C-12 to C-18
-
-
-
additional information
?
-
-
substrate specificities of isozymes and chimeric mutants, kcat is strongly influenced by substrate chain length and double bonds
-
-
?
additional information
?
-
Elaeis sp.
-
the recombinant enzyme hydrolyzes medium- and long-chain acyl-CoAs with a 10fold lower activity compared to acyl-[acyl-carrier-protein]s
-
-
?
additional information
?
-
-
the substrate specificity determinants are located at the N-terminus of the isozymes, kcat is strongly influenced by substrate chain length and double bonds
-
-
?
additional information
?
-
enzyme is the chain-length-determining enzyme in de novo biosynthesis of plant fatty acids
-
-
?
additional information
?
-
-
the recombinant enzyme mediates polyhydroxyalkanoate biosynthesis to expressing Escherichia coli, biosynthesis pathway overview
-
-
?
additional information
?
-
-
the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds, substrate specificity of recombinant enzyme, the enzyme shows preference for monounsaturated acyl-ACPs
-
-
-
additional information
?
-
-
the enzyme is long-chain fatty acid specific
-
-
-
additional information
?
-
FatA enzyme displays low levels of activity on saturated stearoyl- and palmitoyl-[acyl-carrier protein] derivatives
-
-
-
additional information
?
-
-
recombinant ClFATB1 in Escherichia coli has higher specific activities against saturated 16:0- and 18:0-ACPs than on unsaturated 18:1-ACP
-
-
-
additional information
?
-
Helianthus annuus RHA-274
-
the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds, substrate specificity of recombinant enzyme, the enzyme shows preference for monounsaturated acyl-ACPs
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
Q9FT16
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
oleoyl-[acyl-carrier protein] + H2O
oleic acid + acyl-carrier protein
show the reaction diagram
-
role in fatty acid biosynthesis
-
?
oleoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
endpoint of de novo fatty acid synthesis in plastids
-
-
-
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
-
preferred substrate
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
Q9XEL9
preferred substrate
-
?
oleoyl-[acyl-carrier protein] + H2O
[acyl-carrier protein] + oleate
show the reaction diagram
Helianthus annuus RHA-274
-
preferred substrate
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
-
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitic acid + acyl-carrier protein
show the reaction diagram
Elaeis sp.
-
enzyme plays an important role in the control of seed-oil composition, determination of fatty acid chain growth
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
best substrate
-
?
palmitoyl-[acyl-carrier protein] + H2O
palmitate + acyl-carrier protein
show the reaction diagram
-
best substrate, role in fatty acid biosynthesis in flowers and seeds, overview about the fatty acid composition of the plants
-
?
stearoyl-[acyl-carrier protein] + H2O
stearic acid + acyl-carrier protein
show the reaction diagram
Q9SQI3
-
-
?
stearoyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
synthesis of fatty acids for wax biosynthesis
-
-
-
acyl-[acyl-carrier protein] + H2O
?
show the reaction diagram
-
role in controlling the acyl flux through the eucaryotic and procaryotic pathway in plants
-
-
-
additional information
?
-
Q9FT16
enzyme is the chain-length-determining enzyme in de novo biosynthesis of plant fatty acids
-
-
?
additional information
?
-
-
the recombinant enzyme mediates polyhydroxyalkanoate biosynthesis to expressing Escherichia coli, biosynthesis pathway overview
-
-
?
additional information
?
-
Helianthus annuus, Helianthus annuus RHA-274
-
the enzyme is responsible for the biosynthesis of storage and membrane lipids in seeds
-
-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoate)
-
-
5,5'-dithiobis(2-nitrobenzoate)
-
-
diethyldicarbonate
-
-
N-ethylmaleimide
-
-
oleoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
palmitoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
stearoyl-[acyl-carrier protein]
-
competitive to other acyl-[acyl-carrier protein] substrates
iodoacetamide
-
-
additional information
-
no inhibition by phenylmethylsulfonylfluoride
-
additional information
-
no inhibition by phenylmethylsulfonylfluoride
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0006
(9Z)-hexadec-9-enoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.0023
decanoyl-[acyl-carrier protein]
-
-
0.0031
decanoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.004
decanoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0049
dodecanoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.0146
lauroyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0029
myristoleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.0139
myristoleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.0028
myristoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.004
myristoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.0059
myristoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0067
myristoyl-[acyl-carrier protein]
-
-
0.0089
myristoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.0024
octanoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.029
octanoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.00008
oleoyl-[acyl-carrier protein]
pH 8.0, 22C
0.00042
oleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00067
oleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00074
oleoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 30 days after flowering
0.0008
oleoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 15 days after flowering
0.0013
oleoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0014
oleoyl-[acyl-carrier protein]
-
pH 8.0, 22C
0.0021
oleoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.0026
oleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.00297
oleoyl-[acyl-carrier protein]
pH 8.0, 22C
0.0031
oleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.004
oleoyl-[acyl-carrier protein]
-
pH 9, 37C
0.0048
oleoyl-[acyl-carrier protein]
-
-
0.0074
oleoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.008
oleoyl-[acyl-carrier protein]
-
enzyme form PO-FAT, pH 9.0, 37C
3
oleoyl-[acyl-carrier protein]
-
pH 9.0, 35C
0.0006
oleoyl-[acyl-carrier-protein]
-
pH 8, 20C, mutant L118W; pH 8, 20C, mutant T182W
0.0009
oleoyl-[acyl-carrier-protein]
-
pH 8, 20C, wild-type
0.0022
oleoyl-[acyl-carrier-protein]
-
pH 8, 20C, mutant Q215W
0.0031
oleoyl-[acyl-carrier-protein]
-
pH 8, 20C
0.0039
oleoyl-[acyl-carrier-protein]
-
pH 8, 20C
0.0212
oleoyl-[acyl-carrier-protein]
-
pH 8, 20C, mutant M206W
0.0001
palmitoleoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant Q215W
0.0002
palmitoleoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant M206W
0.0003
palmitoleoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant L118W; pH 8, 20C, wild-type
0.00035
palmitoleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00035
palmitoleoyl-[acyl-carrier protein]
pH 8.0, 22C
0.0005
palmitoleoyl-[acyl-carrier protein]
-
pH 8, 20C
0.0006
palmitoleoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant T182W
0.00086
palmitoleoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.0022
palmitoleoyl-[acyl-carrier protein]
-
pH 8, 20C
0.0026
palmitoleoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.0049
palmitoleoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.00551
palmitoleoyl-[acyl-carrier protein]
pH 8.0, 22C
0.00028
palmitoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 15 days after flowering
0.00053
palmitoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 30 days after flowering
0.00054
palmitoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.00056
palmitoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.0007
palmitoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant L118W
0.0009
palmitoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant Q215W; pH 8, 20C, mutant T182W
0.00096
palmitoyl-[acyl-carrier protein]
pH 8.0, 22C
0.001
palmitoyl-[acyl-carrier protein]
-
pH 8, 20C, wild-type
0.0012
palmitoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant M206W
0.0013
palmitoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.0015
palmitoyl-[acyl-carrier protein]
-
pH 8, 20C
0.0036
palmitoyl-[acyl-carrier protein]
-
-
0.0036
palmitoyl-[acyl-carrier protein]
-
enzyme form PO-FAT, pH 9.0, 37C
0.0036
palmitoyl-[acyl-carrier protein]
pH 8.0, 22C
0.0038
palmitoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.0041
palmitoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.0043
palmitoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.0049
palmitoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.0066
palmitoyl-[acyl-carrier protein]
-
pH 8, 20C
0.00034
stearoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 15 days after flowering
0.0004
stearoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant M206W
0.0005
stearoyl-[acyl-carrier protein]
-
pH 8, 20C
0.00054
stearoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.0007
stearoyl-[acyl-carrier protein]
-
pH 8, 20C, wild-type
0.00088
stearoyl-[acyl-carrier protein]
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.0009
stearoyl-[acyl-carrier protein]
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.00092
stearoyl-[acyl-carrier protein]
-
pH 8.5, 25C, 30 days after flowering
0.001
stearoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant L118W
0.0015
stearoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant T182W
0.0025
stearoyl-[acyl-carrier protein]
-
pH 8, 20C, mutant Q215W
0.0025
stearoyl-[acyl-carrier protein]
-
medium-chain acyl-ACP thioesterase
0.003
stearoyl-[acyl-carrier protein]
-
isozyme FatB, pH 8.0, 22C
0.003
stearoyl-[acyl-carrier protein]
-
pH 9, 37C
0.0034
stearoyl-[acyl-carrier protein]
-
enzyme form PO-FAT, pH 9.0, 37C
0.0039
stearoyl-[acyl-carrier protein]
-
pH 8, 20C
0.005
stearoyl-[acyl-carrier protein]
-
isozyme FatA, pH 8.0, 22C
0.00563
stearoyl-[acyl-carrier protein]
pH 8.0, 22C
0.0068
stearoyl-[acyl-carrier protein]
-
oleoyl-ACP thioesterase
0.00954
stearoyl-[acyl-carrier protein]
pH 8.0, 22C
0.01
stearoyl-[acyl-carrier protein]
-
-
0.0067
undecanoyl-[acyl-carrier protein]
-
-
0.0166
lauroyl-[acyl-carrier protein]
-
-
additional information
additional information
-
Km is slightly influenced by substrate chain length and double bonds
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.004
myristoleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatA, pH 8.0, 22C
0.22
myristoleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
0.008
myristoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatA, pH 8.0, 22C
0.02
myristoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
0.25
oleoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
0.67
oleoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.98
oleoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
1.23
oleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
1.94
oleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatA, pH 8.0, 22C
2.94
oleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatA, pH 8.0, 22C
6.08
oleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
7.99
oleoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
11.4
oleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.032
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, mutant T182W
0.0854
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, mutant Q215W
0.1258
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, mutant M206W
1
oleoyl-[acyl-carrier-protein]
Camelina sativa
-
pH 8, 20C
2.2
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, mutant L118W
7
oleoyl-[acyl-carrier-protein]
Camelina sativa
-
pH 8, 20C
35.6
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, wild-type
0.0016
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant M206W
0.0062
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant Q215W
0.016
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant T182W
0.06
palmitoleoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
0.1
palmitoleoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
0.12
palmitoleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatA, pH 8.0, 22C
0.15
palmitoleoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
0.22
palmitoleoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.45
palmitoleoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
0.6
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant L118W
0.74
palmitoleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
1.62
palmitoleoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
6.08
palmitoleoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
8
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, wild-type
0.0005
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant M206W
0.002
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant T182W
0.0024
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant Q215W
0.03
palmitoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
0.04
palmitoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatA, pH 8.0, 22C
0.2
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant L118W
0.3
palmitoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
0.41
palmitoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.44
palmitoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
0.46
palmitoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.5
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8.0, 22C, recombinant His-tagged enzyme
1.1
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, wild-type
2.4
palmitoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
4.4
palmitoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
0.0002
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant M206W
0.003
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant T182W
0.0041
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant Q215W
0.15
stearoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatA, pH 8.0, 22C
0.18
stearoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
0.2
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant L118W
0.38
stearoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.4
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8.0, 22C, recombinant His-tagged enzyme
0.4
stearoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
0.56
stearoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
0.6
stearoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
0.8
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, wild-type
0.88
stearoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
0.91
stearoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
6.08
stearoyl-[acyl-carrier protein]
Arabidopsis thaliana
-
isozyme FatB, pH 8.0, 22C
0.9
(9Z)-hexadec-9-enoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8.0, 22C, recombinant His-tagged enzyme
additional information
additional information
Arabidopsis thaliana
-
recombinant chimeric mutants FatAB and FatBA
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
80
oleoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
1933
990
oleoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
1933
12910
oleoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
1933
19030
oleoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
1933
50
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, mutant T182W
67097
300
oleoyl-[acyl-carrier-protein]
Camelina sativa
-
pH 8, 20C
67097
1800
oleoyl-[acyl-carrier-protein]
Camelina sativa
-
pH 8, 20C
67097
3700
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, mutant L118W
67097
5900
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, mutant M206W
67097
39000
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, wild-type
67097
39300
oleoyl-[acyl-carrier-protein]
Helianthus annuus
-
pH 8, 20C, mutant Q215W
67097
28
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant T182W
3103
30
palmitoleoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
3103
30
palmitoleoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
3103
250
palmitoleoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
3103
300
palmitoleoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
3103
1300
palmitoleoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
3103
1800
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant L118W
3103
4630
palmitoleoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
3103
6600
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant M206W
3103
23400
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, wild-type
3103
48000
palmitoleoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant Q215W
3103
3
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant T182W
1598
30
palmitoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
1598
120
palmitoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
1598
200
palmitoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
1598
300
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant L118W
1598
400
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant M206W
1598
600
palmitoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
1598
760
palmitoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
1598
830
palmitoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
1598
1100
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, wild-type
1598
2800
palmitoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant Q215W
1598
2
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant T182W
1770
30
stearoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
1770
90
stearoyl-[acyl-carrier protein]
Macadamia tetraphylla
D2E6T0, D5FFZ2
pH 8.0, 22C
1770
200
stearoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
1770
200
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant L118W
1770
440
stearoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
1770
500
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant M206W
1770
750
stearoyl-[acyl-carrier protein]
Camelina sativa
-
pH 8, 20C
1770
1030
stearoyl-[acyl-carrier protein]
Ricinus communis
A9XK92, A9XUG5
in 50 mM Tris-HCl buffer, pH 8.0, 5 mM DTT, at 22C
1770
1200
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, wild-type
1770
1600
stearoyl-[acyl-carrier protein]
Helianthus annuus
-
pH 8, 20C, mutant Q215W
1770
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.043
palmitoyl-[acyl-carrier protein], pH 8.0, 22C
0.144
-
enzyme from leaf epidermis
0.18
-
substrate stearoyl-[acyl-carrier protein], pH 9, 37C
0.262
palmitoleoyl-[acyl-carrier protein], pH 8.0, 22C
0.324
stearoyl-[acyl-carrier protein], pH 8.0, 22C
0.437
oleoyl-[acyl-carrier protein], pH 8.0, 22C
0.69
-
seed 65 days after flowering, enzyme form PO-FAT
0.744
palmitoleoyl-[acyl-carrier protein], pH 8.0, 22C
0.778
palmitoyl-[acyl-carrier protein], pH 8.0, 22C
1.45
stearoyl-[acyl-carrier protein], pH 8.0, 22C
1.61
oleoyl-[acyl-carrier protein], pH 8.0, 22C
11
-
isozyme AH1
15
-
recombinant enzyme in Escherichia coli, substrate lauroyl-CoA
192
-
purified enzyme form PO-FAT
1500
-
recombinant enzyme in Escherichia coli, substrate lauroyl-[acyl-carrier-protein]
additional information
-
-
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 9
-
medium-chain acyl-ACP thioesterase
8
-
assay at
8
-
assay at
8.5
-
assay at
8.5
-
enzyme from leaf epidermis
9
-
enzyme form PO-FAT
9.3
-
oleoyl-ACP thioesterase
9.8
-
isozymes AH1, AH2
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.8 - 9
-
medium-chain acyl-ACP thioesterase
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
20
-
assay at
20
-
assay at
22
-
room temperature, assay at
22
-
assay at room temperature
25
-
assay at
36
-
around 35-37C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.57
-
sequence analysis
6.7
calculated from sequence
7
calculated from sequence
7
-
chromatofocusing
8.1
-
amino acid sequence calculation
8.2
-
enzyme form LC-FAT, chromatofocusing
9.7
-
enzyme form PO-FAT, chromatofocusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
developing fruit
Manually annotated by BRENDA team
Elaeis sp.
-
-
Manually annotated by BRENDA team
-
high expression; specifically expressed in flower petals
Manually annotated by BRENDA team
developing kernel; developing kernel
Manually annotated by BRENDA team
Elaeis sp.
-
high level expression
Manually annotated by BRENDA team
Populus tomentosa Carr
-
-
-
Manually annotated by BRENDA team
-
stearoyl-ACP thioestersae is predominantly expressed in epidermis
Manually annotated by BRENDA team
Elaeis sp.
-
15-18 weeks old tissue, high level expression, 8-12 weeks old tissue, low level expression
Manually annotated by BRENDA team
-
lowest level
Manually annotated by BRENDA team
-
specifically expressed in root endodermal and peridermal cells
Manually annotated by BRENDA team
Populus tomentosa Carr
-
-
-
Manually annotated by BRENDA team
-
developing, period from 45 to 93 days after flowering
Manually annotated by BRENDA team
-
highest enzyme activity during seed development 15 days after flowering
Manually annotated by BRENDA team
-
developing, fatty acid composition, overview
Manually annotated by BRENDA team
; CcFATB1 is seed specific
Manually annotated by BRENDA team
-
highest level
Manually annotated by BRENDA team
-
highest expression
Manually annotated by BRENDA team
-
; strong expression in the immature seeds harvested at 60 days after pegging
Manually annotated by BRENDA team
Helianthus annuus RHA-274
-
-
-
Manually annotated by BRENDA team
Elaeis sp.
-
germinating, low level expression
Manually annotated by BRENDA team
15-60 days after flowering
Manually annotated by BRENDA team
expression of PtFATB is in a constitutive fashion
Manually annotated by BRENDA team
Populus tomentosa Carr
-
expression of PtFATB is in a constitutive fashion
-
Manually annotated by BRENDA team
-
high expression; specifically expressed in stem epidermal cells
Manually annotated by BRENDA team
additional information
Elaeis sp.
-
no expression in kernel tissues
Manually annotated by BRENDA team
additional information
no detectable mRNA in root, leaf, and hypocotyl
Manually annotated by BRENDA team
additional information
-
tissue-specific expression pattern
Manually annotated by BRENDA team
additional information
CcFATB2 is expressed ubiquitously
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
putative chloroplast transit peptide at the N-terminal
Manually annotated by BRENDA team
-
nuclear gene encoded, targeted to the plastids, soluble
Manually annotated by BRENDA team
additional information
Elaeis sp.
-
the gene contains a putative transit peptide sequence
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
28000
-
medium-chain acyl-ACP thioesterase, gel filtration
81006
33400
-
calculated from cDNA
732615
34800
-
calculated from cDNA
732615
36800
recombinant histidine-tagged FatB, gel filtration
710244
37000
-
SDS-PAGE
732664
37250
-
calculation from sequence of cDNA
81008
37520
recombinant histidine-tagged FatA, gel filtration
710244
40500
-
SDS-PAGE
731878
41960
-
precusor form, encoded by the pNL2 gene, calculation from sequence of cDNA
81007
41980
-
precusor form, encoded by the pNL3 gene, calculation from sequence of cDNA
81007
42200
FatA open reading frames, calculated from sequence
710244
45000
-
gel filtration
653557
46500
FatB open reading frames, calculated from sequence
710244
48080
-
sequence analysis
710647
51000
-
isozymes AH1, AH2, TSKgel G3000SW gel filtration
81009
53000
-
oleoyl-ACP thioesterase, gel filtration
81006
57000
-
isozyme AH3, TSKgel G3000SW gel filtration
81009
59000
-
isozyme AH1, gel filtration
81009
61000
-
SDS-PAGE, recombinant protein
731325
70000 - 80000
-
gel filtration
80999
72000
-
gel filtration
666658
72000
-
SDS-PAGE
732192
78000
-
gel filtration
716463
84000
-
enzyme from leaf epidermis, gel filtration
81001
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
Elaeis sp.
-
x * 66000, recombinant glutathione S-transferase fusion protein, SDS-PAGE
?
-
x * 41200, about, amino acid sequence calculation, x * 36900, recombinant His-tagged enzyme without signal peptide, SDS-PAGE
?
x * 42200, FatA open reading frames, calculated from sequence; x * 46500, FatA open reading frames, calculated from sequence
?
-
x * 48080, calculated from sequence
?
x * 45900, calculated; x * 46200, calculated
?
Helianthus annuus RHA-274
-
x * 41200, about, amino acid sequence calculation, x * 36900, recombinant His-tagged enzyme without signal peptide, SDS-PAGE
-
dimer
-
2 * 38000, SDS-PAGE
dimer
-
2 * 40000, enzyme from leaf epidermis, SDS-PAGE
dimer
-
2 * 35000, SDS-PAGE
homodimer
-
2 * 40000, Western blot analysis
monomer
-
1 * 42500-44000, SDS-PAGE
additional information
sequence-predicted three-dimensional structure, comprises two helix/4-stranded sheet domains, the N-terminal domain contains residues that affect specificity, and the C-terminal domain contains the catalytic residues, modelling, PDB code 1XXY, structure comparisons to other plant thioesterases, overview
additional information
-
secondary structure and predicted three-dimensional structure, modeling and analysis
additional information
Helianthus annuus RHA-274
-
secondary structure and predicted three-dimensional structure, modeling and analysis
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
proteolytic modification
-
the 40 kDa protein is cleaved to a 34 kDa protein within 3 h. This degradation does not change activity, it is unknown whether this degradation is of physiological significance
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
35
-
activity relatively stable at 35C
716463
50
-
20 min stable
80999
60
-
15 min, enzyme form LC-FAT is mostly inactivated, while enzyme form PO-FAT is activated by 15-20%
653557
60
-
60% activity lost after 15 min at 60C, 75% activity lost after 30 min at 60C
716463
70
-
15 min, enzyme form PO-FAT is heat-stable, after 45 min the enzyme retains 30% of the original activity
653557
80
-
15 min, 30% remaining activity
653557
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol, 25%, is required for stability during purification for the enzyme form PO-FAT
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, enzyme form PO-FAT, ammonium sulfate pellet, stable
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from leaf epidermis
-
native enzyme to homogeneity from developing seeds by hydroxyapatite, ion exchange, and [acyl carrier protein] affinity chromatographies, and preparative native PAGE
-
using affinity chromatography
-
partial purification of the isozymes and the recombinant chimeric mutants
-
isozyme AH1
-
two thioesterase activities: medium-chain acyl-ACP thioesterase and oleoyl-ACP thioesterase
-
enzyme form PO-FAT, 499fold
-
recombinant His-tagged enzyme from Escherichia coli by metal affinity chromatography
-
using metal affinity chromatography
-
immobilized metal ion affinity chromatography (Ni2+); immobilized metal ion affinity chromatography (Ni2+)
ammonium sulfate precipitation, ion exchange chromatography (DEAE), [acyl-carrier protein]-affinity chromatography
-
var. Fuerte
-
to homogeneity in a single step by gel filtration; to homogeneity in a single step by gel filtration
partial purification
-
Uc FatB1 and mutants
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in seeds of Arabidopsis thaliana under control of a seed-specific promotor via Agrobacterium tumefaciens infection resulting in accumulation of high amounts of palmitate, anti-sense expression of the cDNA in seeds under control of the S35 promotor from cauliflower mosaic virus leading to reduced enzyme activity in flowers and seeds but not in leaves, tissue-specific expression pattern
-
overexpression in transgenic wheat
-
recombinantly expressed in Escherichia coli
-
expressed in Escherichia coli
-
DNA sequence determination and analysis
expressed in Escherichia coli
-
expression in Escherichia coli
-
expressed in Nicotiana tabacum
-
expressed in Phaeodactylum tricornutum
recombinantly expressed in Escherichia coli
-
expression in Escherichia coli
-
overexpression of wild-type isozymes and chimeric mutants in Escherichia coli Bl21(DE3), the chimeric mutants are constructed by combination of the N-terminus of FatA with the C-terminus of FatB resulting in FatAB, or vice versa resulting in FatBA
-
overexpression of CcFATB1 in developing seeds of Arabidopsis partially redirects fatty acid synthesis toward medium-chain FA (12:0, 14:0, and 16:0). Overexpression of CcFATB1 cDNA complemented the low-palmitate (16:0) but not the low-stearate (18:0) phenotype in leaves and seeds of an Arabidopsis fatb-2 mutant; overexpression of CcFATB2 in developing seeds of Arabidopsis complements the low-palmitate (16:0) and the low-stearate (18:0) phenotype in leaves and seeds of an Arabidopsis fatb-2 mutant
expression in Escherichia coli
DNA and amino acid sequence determination and analysis, construction of a cDNA library from kernel DNA
single gene, partial DNA sequence determination and DNA sequence analysis, expression in Escherichia coli BL21 (DE3) as glutathione S-transferase fusion protein
Elaeis sp.
-
expression of FatB as soluble enzyme in diverse Escherichia coli strains and in vitro transcription and translation lead to 2 different polypeptides by alternative initiation, a full-length 46 kDa form and a truncated 35 kDa form
expressed in Tobacco, Arabidopsis thaliana and Escherichia coli
-
gene fatA1, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression as soluble His-tagged enzyme in Escherichia coli strain BL21 and DH5alpha, lipid analysis in recombinant Escherichia coli cells
-
coding region (including the chloroplast targeting peptide) of FATB1 cDNA amplified, then ligated into the 2S::GUS plasmid vector, using XbaI/SacI to place the gusA gene behind the seed-specific promoter. The 2S::JcFATB1 construct transferred into Agrobacterium tumefaciens GV3101, and Arabidopsis plants for overexpression
-
expressed in Escherichia coli K27fadD88
-
expressed in Escherichia coli
-
a His-tagged version expressed in Escherichia coli XL-1 blue; a His-tagged version expressed in Escherichia coli XL-1 blue
expression in Escherichia coli. The ectopically expressed MlFatB gene causes alteration in the fatty acid profile of Escherichia coli cells
PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatA overexpressed in Escherichia coli XL1-Blue; PCR fragments cloned into the pMBL-T vector, subcloned into the BamHI-HindIII or SacI-KpnI sites of the pQE-80L vector. Resulting construct pQERcFatB overexpressed in Escherichia coli XL1-Blue
expressed in Escherichia coli
-
expressed in Escherichia coli MG1655
-
expressed in Phaeodactylum tricornutum
expression of medium-chain acyl-acyl carrier protein thioesterase in Escherichia coli
-
expression of UcFatB1 and mutants in Escherichia coli
-
functional expression in Escherichia coli, diverse recombinant mutant strains
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
highly expressed in developing seed endosperm; highly expressed in developing seed endosperm and leaf
-
is most strongly expressed in seeds, in which its expression peaks in late developmental stages
-
highest expression in the developing seeds 75 days after flowering
-
transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation; transcript accumulation profile is temporally regulated during embryo development. Displays maximum level of transcription in the period of maximum oil accumulation
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
K216A
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
K216E
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
L118W
-
mutant displays three orders of magnitude lower catalytic activity compared to wild-type. Km value similar to wild-type
M206W
-
mutant displays lower catalytic activity compared to wild-type. Km value similar to wild-type, only for substrate 18:1-ACP Km value highly increased compared to wild-type
Q215W
-
mutant displays equivalent or improved activities towards all substrates with marked 2 and 2.6fold increased activities for palmitoleoyl-ACP and palmitoyl-ACP, respectively, compared to wild-type. Expression of mutant in Nicotiana benthamiana leads to a higher average values of acyl-CoA. Expressed in Arabidopsis mutant displays displays a 40% decrease in oil content relative to wild-type. Mutant produces an increase in the content of saturated fatty acids, palmitic and stearic acids, with a concomitant decrease in oleic acid derivatives
R143A/K144A
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
R143E/K144E
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
R212A/R213A
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
R212E/R213E
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
T182W
-
mutant displays lower catalytic activity compared to wild-type. Km value similar to wild-type. Leaf triacylglycerols are increased 2 to 4fold when expressed in Nicotiana benthamiana compared to expression of mutant T182W and wild-type, respectively
K216A
Helianthus annuus RHA-274
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
-
R143A/K144A
Helianthus annuus RHA-274
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
-
R143E/K144E
Helianthus annuus RHA-274
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
-
R212A/R213A
Helianthus annuus RHA-274
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
-
C320A
-
no activity
C320S
-
slightly reduced activity
H140A
-
reduced activity
H140R
-
reduced activity
H285A
-
no activity
H285R
-
reduced activity
H345A
-
reduced activity
H345R
-
reduced activity
additional information
-
metabolic and morphological effects of transgenic expression of the enzyme together with the glycerol-3-phosphate acyltransferase from Pisum sativum in wheat plants
R212E/R213E
Helianthus annuus RHA-274
-
site-directed mutagenesis, the mutant shows latered substrate specificity compared to the wild-type enzyme in recombinant Escherichia coli cells, lipid composition, overview
-
additional information
-
using codon-optimized Streptococcus pyogenes acyl-ACP TE (OPSpTE, containing replaced native codons from Escherichia coli MG1655- and W3110-favored codons) to improve the total free fatty acids (FFAs) and to tailor the composition of FFAs, high-specificity production of saturated fatty acids (C12, C14) and unsaturated fatty acids (C18:1 C18:2) is achieved in recombinant Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
the new MbFatB gene isolated from Madhuca butyracea can be used in future for transgenic development of oil-seed Brassica, a widely cultivated crop that expresses predominantly oleoyl-ACP thioesterase (FatA) in its seed tissue and has high amount of unwanted erucic acid in edible oil in order to alter the fatty acid profile in a desirable way
industry
-
FATB1 can function as a saturated acyl-ACP TE and may potentially modify the seed oil to increase its levels of palmitate
industry
contribution of FatA to the synthesis of castor oil; contribution of FatB to the synthesis of castor oil