Crystallization (Comment) | Organism |
---|---|
hanging drop method, crystallographic analysis of wild-type and S136A mutant enzyme, 1.8 A resolution data | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the C-terminal domain reduces the optimal temperature from 90 to 80°C. The lack of the C-terminal domain also causes the protein to be less thermostable. However, the deletion also changes the optimal pH and pH stability of the recombinant enzyme. It reduces the optimal pH from 10.0 to 9.0 and causes the protein to be less pH stable. Without the C-terminal domain, the enzyme loses its function to hydrolyze long-chain ester substrates | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28511 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl octanoate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl palmitate + H2O | - |
Archaeoglobus fulgidus | 4-nitrophenol + palmitate | - |
? | |
tricaprylin + 3 H2O | the apparent rate of hydrolysis correlates with the degree of micellar formation. Consistent with observations from other lipases, the lid conformation may change from the closed to the open form in the presence of lipid interface. Together with the evidence of the lid structure in in this enzyme, it is classified as a lipase rather than as a carboxylesterase | Archaeoglobus fulgidus | 3 octanoate + glycerol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Archaeoglobus fulgidus |
90 | - |
- |
Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | 30°C: about 50% of maximal activity, 90°C: optimum, recombinant enzyme | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
30 min, about 5% loss of activity | Archaeoglobus fulgidus |
90 | - |
30 min, about 95% loss of activity | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Archaeoglobus fulgidus |
10 | - |
at 60°C | Archaeoglobus fulgidus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | pH 5.0: about 55% of maximal activity, pH 11.0: about 85% of maximal activity | Archaeoglobus fulgidus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
60°C, activity after incubation for 2 h at pH 5.0 is about 20% lower compared to activity at pH 10.0 | Archaeoglobus fulgidus |
10 | - |
60°C, 2 h, pH of highest stability | Archaeoglobus fulgidus |
11 | - |
60°C, activity after incubation for 2 h at pH 5.0 is about 30% lower compared to activity at pH 10.0 | Archaeoglobus fulgidus |