Literature summary for 3.1.1.3 extracted from

Carrasco-Lopez, C.; Godoy, C.; de las Rivas, B.; Fernandez-Lorente, G.; Palomo, J.M.; Guisan, J.M.; Fernandez-Lafuente, R.; Martinez-Ripoll, M.; Hermoso, J.A.
Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganism Bacillus thermocatenulatus (2008), Acta Crystallogr. Sect. F, 64, 1043-1045.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21 (DE3)	Geobacillus thermocatenulatus

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, mixture of 13% 2-methyl-2,4-pentanediol and 0.2 M ammonium acetate in 0.05 M sodium citrate pH 5.5-5.6, at 18°C	Geobacillus thermocatenulatus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
43000	-	SDS-PAGE	Geobacillus thermocatenulatus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus thermocatenulatus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
octyl-Sepharose column chromatography, Q-Sepharose column chromatography, and SP-550 Toyopearl column chromatography	Geobacillus thermocatenulatus

Synonyms

Synonyms	Comment	Organism
BTL2	-	Geobacillus thermocatenulatus
lipase 2	-	Geobacillus thermocatenulatus
triacylglycerol acylhydrolase	-	Geobacillus thermocatenulatus

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Release 2023.1 (January 2023)