Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Staphylococcus xylosus |
Protein Variants | Comment | Organism |
---|---|---|
G311D | site-directed mutagenesis, the mutant shows reduced activity and a distinct pH profile compared to the wild-type enzyme | Staphylococcus xylosus |
G311K | site-directed mutagenesis, the mutant shows reduced activity and a distinct pH profile compared to the wild-type enzyme | Staphylococcus xylosus |
G311L | site-directed mutagenesis, the mutant shows reduced activity but increased pH and thermal stability compared to the wild-type enzyme, the mutant also shows phospholipase activity with phosphatidylcholine in contrast to the wild-type enzyme | Staphylococcus xylosus |
G311W | site-directed mutagenesis, inactive mutant | Staphylococcus xylosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311D | Staphylococcus xylosus | |
5 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311D | Staphylococcus xylosus | |
13.9 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311K | Staphylococcus xylosus | |
18.9 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311L | Staphylococcus xylosus | |
20.4 | - |
tributyrin | pH 8.0, 37°C, recombinant wild-type enzyme | Staphylococcus xylosus | |
27.8 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311K | Staphylococcus xylosus | |
35.7 | - |
olive oil | pH 8.0, 37°C, recombinant wild-type enzyme | Staphylococcus xylosus | |
100 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311L | Staphylococcus xylosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, recombinant enzyme, SDS-PAGE | Staphylococcus xylosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus xylosus | Q2TPV1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Staphylococcus xylosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | residue Gly311 is essential for catalytic activity | Staphylococcus xylosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
purified recombinant mutant G311K, substrate olive oil | Staphylococcus xylosus |
25 | - |
purified recombinant mutant G311D, substrate olive oil | Staphylococcus xylosus |
30 | - |
purified recombinant mutant G311K, substrate tributyrin | Staphylococcus xylosus |
100 | - |
purified recombinant mutant G311D, substrate tributyrin | Staphylococcus xylosus |
220 | - |
purified recombinant mutant G311L, substrate olive oil | Staphylococcus xylosus |
280 | - |
purified recombinant mutant G311L, substrate tributyrin | Staphylococcus xylosus |
850 | - |
purified recombinant wild-type enzyme, substrate olive oil | Staphylococcus xylosus |
1500 | - |
purified recombinant wild-type enzyme, substrate tributyrin | Staphylococcus xylosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
tributyrin + H2O | - |
Staphylococcus xylosus | dibutyrin + butyrate | - |
? | |
trioctanoin + H2O | - |
Staphylococcus xylosus | dioctanoin + octanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, recombinant enzyme, SDS-PAGE | Staphylococcus xylosus |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Staphylococcus xylosus |
SXL | - |
Staphylococcus xylosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus xylosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | - |
Staphylococcus xylosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant mutant G311L shows increased half-life of 280 min, while the mutant G311D and G311K show 30% and 35% remaining activity, respectively, after 30 min at 50°C | Staphylococcus xylosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311K | Staphylococcus xylosus | |
30 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311K | Staphylococcus xylosus | |
37.9 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311D | Staphylococcus xylosus | |
50 | - |
olive oil | pH 8.0, 37°C, recombinant mutant G311L | Staphylococcus xylosus | |
75.8 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311D | Staphylococcus xylosus | |
250 | - |
tributyrin | pH 8.0, 37°C, recombinant mutant G311L | Staphylococcus xylosus | |
500 | - |
olive oil | pH 8.0, 37°C, recombinant wild-type enzyme | Staphylococcus xylosus | |
1500 | - |
tributyrin | pH 8.0, 37°C, recombinant wild-type enzyme | Staphylococcus xylosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
mutant G311D | Staphylococcus xylosus |
8 | - |
wild-type enzyme | Staphylococcus xylosus |
9.5 | - |
mutant G311K | Staphylococcus xylosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | - |
Staphylococcus xylosus |