Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Colipase | required | Gallus gallus | |
additional information | CPL presents the interfacial activation phenomenon determined with tripropionin or vinyl ester | Gallus gallus | |
Na-deoxycholate | bile salts are required | Gallus gallus |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and anaylsis | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | amphipathic protein, the inhibition is fully reversible by addition of bile salts and colipase | Gallus gallus | |
Triton X-100 | the inhibition is fully reversible by addition of bile salts and colipase | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49401 | - |
1 * 50000, SDS-PAGE, 1 * 49401, sequence calculation | Gallus gallus |
50000 | - |
gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | Q0QJZ3 | fragment | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | determination with the anthrone-sulfuric acid method using glucose as standard | Gallus gallus |
Purification (Comment) | Organism |
---|---|
native enzyme 94.7fold from pancreas by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and again anion exchange chromatography to homogeneity | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9000 | - |
purified native enzyme, using tributyrin as substrate in the presence of 4 mM Na-deoxycholate and an excess of colipase. | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
olive oil + H2O | the enzyme can tolerate the accumulation of long-chain free fatty acids at the interface when olive oil emulsion is used as substrate in the absence of bile salts and colipase | Gallus gallus | ? | - |
? | |
tributyrin + H2O | - |
Gallus gallus | dibutyrin + butyrate | - |
? | |
tripropionin + H2O | - |
Gallus gallus | dipropionin + propionate | - |
? | |
vinyl propionate + H2O | - |
Gallus gallus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE, 1 * 49401, sequence calculation | Gallus gallus |
More | building of a 3D structure model of CPL with the catalytic pocket using the human pancreatic lipase as template, overview | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
chicken pancreatic lipase | - |
Gallus gallus |
CPL | - |
Gallus gallus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Gallus gallus |