Activating Compound | Comment | Organism | Structure |
---|---|---|---|
potassium acetate | 20-200 mM, 4-5fold increase in activity | Oryza sativa | |
Sodium acetate | 20 mM, 2fold increase in activity, no effect at higher concentration at pH 7.5 | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | about 70% inhibition at 2 mM | Oryza sativa | |
CHAPS | inhibitory at 0.5 mM | Oryza sativa | |
Cu2+ | about 70% inhibition at 2 mM | Oryza sativa | |
Digitonin | inhibitory at 0.5 mM | Oryza sativa | |
diisopropyl fluorophosphate | inhibits both lipase and phospholipase activities of the enzyme | Oryza sativa | |
Mg2+ | about 70% inhibition at 2 mM | Oryza sativa | |
additional information | no inhibition by Ca2+, Triton X-100 and NP-40 decreased the activity marginally at lower concentrations and then restorde the activity at 4 mM | Oryza sativa | |
SDS | 50% inhibition at 2mM | Oryza sativa | |
Zn2+ | about 70% inhibition at 2 mM | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
phosphocholine | pH 7.5, 37°C | Oryza sativa | |
6.71 | - |
triolein | pH 7.5, 37°C | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
9400 | - |
gel filtration and MALDI | Oryza sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Oryza sativa |
Purification (Comment) | Organism |
---|---|
7.6fold to homogeneity | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed coat | - |
Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00012 | - |
purified enzyme, phospholipase activity | Oryza sativa |
0.38 | - |
purified enzyme, lipase activity | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacylglycerol + H2O | - |
Oryza sativa | fatty acid + acylglycerol | - |
? | |
additional information | enzyme also provides phospholipase A2 activity, it preferentially hydrolyzes the sn-2-position of the substrate | Oryza sativa | ? | - |
? | |
phosphatidylethanolamine + H2O | - |
Oryza sativa | ? | - |
? | |
phosphocholine + H2O | - |
Oryza sativa | ? | - |
? | |
triolein + H2O | enzyme shows no positional specificity towards the triacylglycerol | Oryza sativa | diolein + oleate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Oryza sativa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Oryza sativa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | completely stable up to 40°C, 65% remaining activity at 60°C after 1 h, 76°C is the transition temperature, the enzyme is still active and retains most of its secondary structure at 90°C, 34% remaining activity at 90°C after 15 min pretreatment at 90°C, 16% remaining activity at 37°C after 15 min pretreatment at 90°C | Oryza sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
11 | - |
- |
Oryza sativa |