Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | inhibition at 10-30 mM | Aspergillus niger | |
chymotrypsin | proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme | Aspergillus niger | |
Cu2+ | strong inhibition, 40% residual activity at 50 mM | Aspergillus niger | |
endoprotease Glu-C | proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme | Aspergillus niger | |
Fe2+ | strong inhibition, 30% residual activity at 50 mM | Aspergillus niger | |
Fe3+ | strong inhibition, 25% residual activity at 50 mM | Aspergillus niger | |
additional information | no inhibition by EDTA 50 mM | Aspergillus niger | |
papain | proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme | Aspergillus niger | |
thrombin | proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme | Aspergillus niger | |
Trypsin | proteolytic cleavage only of the heat-denatured enzyme, not of the native enzyme | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity is not affected by Mg2+, K+, Na+, Ca2+, Mn2+, NH4+, and Zn2+ up to 1.0 M | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35500 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 2.8% sugar, which is not essentail for activity | Aspergillus niger |
Purification (Comment) | Organism |
---|---|
50fold, to homogeneity | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
208.2 | - |
purified enzyme | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate is olive oil, the enzyme is 1,3-specific | Aspergillus niger | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | - |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | - |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
60 min stable | Aspergillus niger |
60 | - |
50% remaining activity after 60 min | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | - |
Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | isoelectric focusing | - |
4.4 |