Protein Variants | Comment | Organism |
---|---|---|
W308F | mutant enzyme is fully active, thermal stability is slightly decreased with respect to wild-type enzyme | Saccharomyces cerevisiae |
W308F/W333F | mutant enzyme is fully active, thermal stability is slightly decreased with respect to wild-type enzyme | Saccharomyces cerevisiae |
General Stability | Organism |
---|---|
enzyme shows highest thermal stability in ternary complex with 3-phosphoglycerate and MgADP-, due to domain closure | Sus scrofa |
enzyme shows highest thermal stability in ternary complex with 3-phosphoglycerate and MgADP-, due to domain closure | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Sus scrofa | ADP + 3-phospho-D-glyceroyl phosphate | - |
? | |
ATP + 3-phospho-D-glycerate | - |
Saccharomyces cerevisiae | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-phosphoglycerate kinase | - |
Sus scrofa |
3-phosphoglycerate kinase | - |
Saccharomyces cerevisiae |
PGK | - |
Sus scrofa |
PGK | - |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
midpoint temperature Tm, without a ligand, carboxamidomethylated enzyme, value determined by differential scanning calometry | Sus scrofa |
48 | - |
midpoint temperature Tm, with MgATP2- as ligand, carboxamidomethylated enzyme, value determined by differential scanning calometry | Sus scrofa |
48 | - |
midpoint temperature Tm, without a ligand, mutant enzyme W308F/W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
49 | - |
midpoint temperature Tm, with MgADP- as ligand, carboxamidomethylated enzyme, value determined by differential scanning calometry | Sus scrofa |
49 | - |
midpoint temperature Tm, with MgATP2- as ligand, mutant enzyme W308F/W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
51 | - |
midpoint temperature Tm, with 3-phosphoglycerate and MgADP- as ligand, carboxamidomethylated enzyme | Sus scrofa |
51 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, mutant enzyme W308F/W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
52 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, carboxamidomethylated enzyme, value determined by differential scanning calometry | Sus scrofa |
52 | - |
midpoint temperature Tm, with MgADP- as ligand, mutant enzyme W308F/W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
53 | - |
midpoint temperature Tm, without a ligand, mutant enzyme W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
53 | - |
midpoint temperature Tm, without a ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
54 | - |
midpoint temperature Tm, mutant enzyme W308F/W333F, with 3-phosphoglycerate and MgADP- as ligand, value determined by differential scanning calometry | Saccharomyces cerevisiae |
55 | - |
midpoint temperature Tm, with MgATP2- as ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
56 | - |
midpoint temperature Tm, with MgATP2- as ligand, mutant enzyme W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
56 | - |
midpoint temperature Tm, without a ligand, wild-type enzyme, value determined by differential scanning calometry | Saccharomyces cerevisiae |
57 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, mutant enzyme W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
57 | - |
midpoint temperature Tm, with MgADP- as ligand, mutant enzyme W333F, value determined by differential scanning calometry | Saccharomyces cerevisiae |
57 | - |
midpoint temperature Tm, with MgADP- as ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
58 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
59 | - |
midpoint temperature Tm, mutant enzyme W333F, with 3-phosphoglycerate and MgADP- as ligand, value determined by differential scanning calometry | Saccharomyces cerevisiae |
59 | - |
midpoint temperature Tm, with 3-phosphoglycerate and MgADP- as ligand, unmodified enzyme, value determined by differential scanning calometry | Sus scrofa |
60 | - |
midpoint temperature Tm, with 3-phosphoglycerate as ligand, wild-type enzyme, value determined by differential scanning calometry | Saccharomyces cerevisiae |
60 | - |
midpoint temperature Tm, with MgATP2- as ligand, wild-type enzyme, value determined by differential scanning calometry | Saccharomyces cerevisiae |
61 | - |
midpoint temperature Tm, with MgADP- as ligand, wild-type enzymne, value determined by differential scanning calometry | Saccharomyces cerevisiae |
63 | - |
midpoint temperature Tm, with 3-phosphoglycerate and MgADP- as ligand, wild-type enzyme, value determined by differential scanning calometry | Saccharomyces cerevisiae |