Information on EC 2.7.2.3 - phosphoglycerate kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.2.3
-
RECOMMENDED NAME
GeneOntology No.
phosphoglycerate kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1-butanol autotrophic biosynthesis (engineered)
-
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Bifidobacterium shunt
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Biosynthesis of antibiotics
-
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Biosynthesis of secondary metabolites
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Calvin-Benson-Bassham cycle
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Carbon fixation in photosynthetic organisms
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formaldehyde assimilation III (dihydroxyacetone cycle)
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gluconeogenesis I
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gluconeogenesis III
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glycerol degradation to butanol
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glycolysis
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Glycolysis / Gluconeogenesis
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glycolysis I (from glucose 6-phosphate)
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glycolysis II (from fructose 6-phosphate)
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glycolysis III (from glucose)
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glycolysis IV (plant cytosol)
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heterolactic fermentation
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Metabolic pathways
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Microbial metabolism in diverse environments
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photosynthesis
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sucrose biosynthesis I (from photosynthesis)
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superpathway of glucose and xylose degradation
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SYSTEMATIC NAME
IUBMB Comments
ATP:3-phospho-D-glycerate 1-phosphotransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9001-83-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bream
-
-
Manually annotated by BRENDA team
Bufo vulgaris
toad
-
-
Manually annotated by BRENDA team
gene PGK1; strain 3153A
SwissProt
Manually annotated by BRENDA team
strain 3153A
SwissProt
Manually annotated by BRENDA team
Chrysophrys guttulatus
red snapper
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
carp
-
-
Manually annotated by BRENDA team
nubian mutant
-
-
Manually annotated by BRENDA team
HM1:IMSS
-
-
Manually annotated by BRENDA team
HM1:IMSS
-
-
Manually annotated by BRENDA team
strain K12
-
-
Manually annotated by BRENDA team
Esox sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Frog
-
-
-
Manually annotated by BRENDA team
NCA 1503
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Pacific halibut
-
-
Manually annotated by BRENDA team
2 isozymes, cytosolic and chloroplastic; cv. Golden Promise
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-
Manually annotated by BRENDA team
strain NCC88
-
-
Manually annotated by BRENDA team
Western grey kangaroo
-
-
Manually annotated by BRENDA team
Eastern grey kangaroo
-
-
Manually annotated by BRENDA team
2 isoforms PGK1 and PGK2, probably cytosolic and chloroplastic form
-
-
Manually annotated by BRENDA team
Mus musculus DBA/2J
DBA/2J
-
-
Manually annotated by BRENDA team
IFO 14297
-
-
Manually annotated by BRENDA team
ATCC 25978T
-
-
Manually annotated by BRENDA team
ringtail possum
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-
Manually annotated by BRENDA team
TACII18
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Pyrococcus woesei Vul 4
strain Vul 4, DSM 3773
SwissProt
Manually annotated by BRENDA team
-
Uniprot
Manually annotated by BRENDA team
strain JCM2524
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-
Manually annotated by BRENDA team
strain JCM2524
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-
Manually annotated by BRENDA team
strain BY4741
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Spirulina geitleri
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
formerly Thermoanaerobium brockii strain Rt8.G4; strain Rt8.G4
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-
Manually annotated by BRENDA team
strain Rt8.G4
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-
Manually annotated by BRENDA team
strain HB8
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-
Manually annotated by BRENDA team
trout
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-
-
Manually annotated by BRENDA team
Vombatus sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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targeted disruption of Pgk2 by homologous recombination eliminates PGK activity in sperm and testis, and severely impairs male fertility, but does not block spermatogenesis. Mating behavior, reproductive organ weights (testis, excurrent ducts, and seminal vesicles), testis histology, sperm counts, and sperm ultrastructure are indistinguishable between Pgk2(-/-) and wild-type mice. Sperm motility and ATP levels are markedly reduced in males lacking PGK2. Pgk2(-/-) males sire occasional pups. Alternative pathways that bypass the PGK step of glycolysis exist. One of these bypass enzymes, acylphosphatase, is active in mouse sperm, perhaps contributing to phenotypic differences between mice lacking GAPDHS or PGK2
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-didehydro-2',3'-dideoxythymidine triphosphate + 3-phospho-D-glycerate
2',3'-didehydro-2',3'-dideoxythymidine diphosphate + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine 5'-triphosphate + 3-phospho-D-glycerate
2',3'-dideoxy-2',3'-didehydro-beta-L(-)-5-fluorodeoxycytidine 5'-diphosphate + 1,3-diphosphoglycerate
show the reaction diagram
2',3'-dideoxy-GTP + 3-phospho-D-glycerate
2',3'-dideoxy-GDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
2'-dATP + 3-phospho-D-glycerate
2'-dADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
2'-deoxycytidine 5'-triphosphate + 3-phospho-D-glycerate
2'-deoxycytidine 5'-diphosphate + 1,3-diphosphoglycerate
show the reaction diagram
2'-dGTP + 3-phospho-D-glycerate
2'-dGDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
2'-dTTP + 3-phospho-D-glycerate
2'-dTDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
3'-deoxy-3'-azidothymidine triphosphate + 3-phospho-D-glycerate
3'-deoxy-3'-azidothymidine diphosphate + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl 1-phosphate
show the reaction diagram
acyclovir triphosphate + 3-phospho-D-glycerate
acyclovir diphosphate + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
ADP + 1,3-bisphosphoglycerate
ATP + 3-phosphoglycerate
show the reaction diagram
-
using the stopped-flow method it is shown that substrate binding kinetics that lead to the formation of the catalytic PGK-1,3-bisphosphoglycerate-ADP complexes are mutually antagonistic with D-ADP, but much less so with L-ADP. A situation that is similar to that for the formation of the abortive PGK-3-phosphoglycerate-ADP complexes
-
-
?
ADP + 3-phospho-D-glyceroyl 1-phosphate
3-phospho-D-glycerate
show the reaction diagram
ADP + 3-phospho-D-glyceroyl 1-phosphate
ATP + 3-phospho-D-glycerate
show the reaction diagram
ADP + 3-phospho-D-glyceroyl phosphate
ATP + 3-phospho-D-glycerate
show the reaction diagram
ATP + 3-phospho-D-glycerate
ADP + 1,3-diphosphoglycerate
show the reaction diagram
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl 1-phosphate
show the reaction diagram
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl phosphate.
show the reaction diagram
beta-L(-)-dioxolanecytidine 5'-triphosphate + 3-phospho-D-glycerate
beta-L(-)-dioxolanecytidine 5'-diphosphate + 1,3-diphosphoglycerate
show the reaction diagram
beta-L-2',3'-dideoxy-3'-thiacytidine 5'-triphosphate + 3-phospho-D-glycerate
beta-L-2',3'-dideoxy-3'-thiacytidine 5'-diphosphate + 1,3-diphosphoglycerate
show the reaction diagram
CTP + 3-phospho-D-glycerate
CDP + 1,3-diphosphoglycerate
show the reaction diagram
D-ADP + 1,3-bisphosphoglycerate
?
show the reaction diagram
-
-
-
-
?
D-ATP + 1,3-bisphospho-D-glycerate
?
show the reaction diagram
-
-
-
-
?
D-ATP + 3-phospho-D-glycerate
D-ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
D-CDP + 1,3-bisphospho-D-glycerate
?
show the reaction diagram
-
-
-
-
?
D-CTP + 3-phospho-D-glycerate
D-CDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
D-GTP + 3-phospho-D-glycerate
D-GDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
dATP + 3-phospho-D-glycerate
dADP + 1,3-diphosphoglycerate
show the reaction diagram
dGTP + 3-phospho-D-glycerate
dGDP + 1,3-diphosphoglycerate
show the reaction diagram
dITP + 3-phospho-D-glycerate
dIDP + 1,3-diphosphoglycerate
show the reaction diagram
GTP + 3-phospho-D-glycerate
GDP + 1,3-diphosphoglycerate
show the reaction diagram
ITP + 3-phospho-D-glycerate
IDP + 1,3-diphosphoglycerate
show the reaction diagram
L-2'-dATP + 3-phospho-D-glycerate
L-2'-dADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
L-2'-dGTP + 3-phospho-D-glycerate
L-2'-dGDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
L-2'-dTTP + 3-phospho-D-glycerate
L-2'-dTDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
L-ADP + 1,3-bisphosphoglycerate
?
show the reaction diagram
-
with L-ADP, the transient burst phase of ATP is more clear-cut than with D-ADP, suggesting that the product release steps are slower with L-ADP than with D-ADP. This is in accordance with the lower kcat measured with L-ADP compared to D-ADP
-
-
?
L-ADP + 3-phospho-D-glyceroyl phosphate
ATP + 3-phospho-D-glycerate
show the reaction diagram
-
L-MgADP is almost as a good substrate for hPGK as the natural D-MgADP
-
-
?
L-ATP + 1,3-bisphospho-D-glycerate
?
show the reaction diagram
-
-
-
-
?
L-ATP + 3-phospho-D-glycerate
L-ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
L-CDP + 1,3-bisphospho-D-glycerate
?
show the reaction diagram
-
-
-
-
?
L-CTP + 3-phospho-D-glycerate
L-CDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
L-GTP + 3-phospho-D-glycerate
L-GDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
L-UTP + 3-phospho-D-glycerate
L-UDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
UTP + 3-phospho-D-glycerate
UDP + 1,3-diphosphoglycerate
show the reaction diagram
UTP + 3-phospho-D-glycerate
UDP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + 3-phospho-D-glyceroyl phosphate
ATP + 3-phospho-D-glycerate
show the reaction diagram
ATP + 3-phospho-D-glycerate
ADP + 3-phospho-D-glyceroyl phosphate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
in the presence of either beta,gamma-imido-adenosine 5'-triphosphate or ADP binding of 3-phospho-D-glycerate is weakened and the contribution of the entropy-factor to its binding is increased relative to the enthalpy factor
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Divalent cation
KCl
-
maximal activation at 100 mM
KH2PO4
NaH2PO4
-
maximal activation at 25-50 mM
NaNO2
-
inhibition at high concentration, acceleration of activity at low concentrations
additional information