Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | the enzyme a possible target for therapeutic intervention in solid tumours | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | a Ca2+/calmodulin-dependent protein kinase | Homo sapiens | |
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | Homo sapiens | - |
ADP + [elongation factor 2] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O00418 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | autophosphorylation site is Thr348 in eEF2K | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
carcinoma cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [elongation factor 2] | - |
Homo sapiens | ADP + [elongation factor 2] phosphate | - |
? | |
additional information | the enzyme performs autophosphorylation on residue Thr348 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
eEF2K | - |
Homo sapiens |
eukaryotic elongation factor 2 kinase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | kinase-dead and other activity-deficient mutants of eEF2K are stabilized, as is a mutant lacking a critical autophosphorylation site (Thr348 in eEF2K), which is thought to be required for eEF2K and other alpha-kinases to achieve their active conformations. In contrast, application of small-molecule eEF2K inhibitors does not stabilize the protein. Achieving an active conformation, rather than eEF2K activity per se, is required for its susceptibility to degradation | Homo sapiens |
metabolism | eEF2K is degraded by a proteasome-dependent pathway in response to genotoxic stress requiring a phosphodegron that includes an autophosphorylation site. The enzyme is also degraded under stress like including acidosis or treatment of cells with 2-deoxyglucose not requiring a phosphodegron | Homo sapiens |
physiological function | eukaryotic elongation factor 2 kinase (eEF2K) is an atypical protein kinase which negatively regulates protein synthesis, is activated under stress conditions and plays a role in cytoprotection, e.g. in cancer cells | Homo sapiens |