Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 1,6-bisphosphate | 20.5% increase of activity at 2.5 mM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H391Y | pyruvate kinase M2 isozyme missense mutation found in cells from Bloom syndrome patients prone to develop cancer, the mutant protein maintains its homotetrameric structure, similar to the wild type protein, but shows a loss of activity of 20%, the mutant shows a 6fold increase in affinity for phosphoenolpyruvate and behaves like a non-allosteric protein with compromised cooperative binding | Homo sapiens |
K422R | pyruvate kinase M2 isozyme missense mutation found in cells from Bloom syndrome patients prone to develop cancer, the mutant protein maintains its homotetrameric structure, similar to the wild type protein, but shows a loss of activity of 75%, the affinity for phosphoenolpyruvate is lost significantly in K422R | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Phe | 23.5% activity left at 1 mM L-Phe in the absence of D-fructose 1,6-bisphosphate | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P14618 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and DEAE-Sepharose column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Homo sapiens | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
pyruvate kinase M2 | isozyme | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
the wild type enzyme and mutant enzyme K422R shows complete loss of activity after 15 min at 4°C, the mutant enzyme H391Y shows 36% loss of activity after 15 min at 4°C | Homo sapiens |
37 | - |
the wild type enzyme shows 56% loss of activity after 3 h at 37°C, the mutant enzyme K422R shows 80% loss of activity after 3 h at 37°C, the mutant enzyme H391Y shows 1% loss of activity after 3 h at 37°C | Homo sapiens |
60 | - |
the wild type enzyme and mutant enzyme K422R show complete loss of activity after 10 min at 60°C, while mutant enzyme H391Y displays only 2% loss of activity after 10 min at 60°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
L-Phe | mutant enzyme K422R, in the absence of D-fructose 1,6-bisphosphate | Homo sapiens | |
0.162 | - |
L-Phe | mutant enzyme K422R, in the presence of 2.5 mM D-fructose 1,6-bisphosphate | Homo sapiens | |
0.176 | - |
L-Phe | wild type enzyme, in the absence of D-fructose 1,6-bisphosphate | Homo sapiens | |
0.261 | - |
L-Phe | wild type enzyme, in the presence of 2.5 mM D-fructose 1,6-bisphosphate | Homo sapiens | |
1.431 | - |
L-Phe | mutant enzyme H391Y, in the absence of D-fructose 1,6-bisphosphate | Homo sapiens | |
1.78 | - |
L-Phe | mutant enzyme H391Y, in the presence of 2.5 mM D-fructose 1,6-bisphosphate | Homo sapiens |