Information on EC 2.7.1.40 - pyruvate kinase

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The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.7.1.40
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RECOMMENDED NAME
GeneOntology No.
pyruvate kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + pyruvate = ADP + phosphoenolpyruvate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
1-butanol autotrophic biosynthesis (engineered)
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anaerobic energy metabolism (invertebrates, cytosol)
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Bifidobacterium shunt
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Biosynthesis of antibiotics
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Biosynthesis of secondary metabolites
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C4 and CAM-carbon fixation
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Entner-Doudoroff pathway II (non-phosphorylative)
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Entner-Doudoroff pathway III (semi-phosphorylative)
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gluconeogenesis II (Methanobacterium thermoautotrophicum)
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glycerol degradation to butanol
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glycolysis
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Glycolysis / Gluconeogenesis
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glycolysis I (from glucose 6-phosphate)
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glycolysis II (from fructose 6-phosphate)
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glycolysis III (from glucose)
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glycolysis IV (plant cytosol)
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glycolysis V (Pyrococcus)
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heterolactic fermentation
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Metabolic pathways
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Microbial metabolism in diverse environments
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mixed acid fermentation
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photosynthetic 3-hydroxybutanoate biosynthesis (engineered)
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Purine metabolism
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Pyruvate metabolism
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Rubisco shunt
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superpathway of glucose and xylose degradation
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SYSTEMATIC NAME
IUBMB Comments
ATP:pyruvate 2-O-phosphotransferase
UTP, GTP, CTP, ITP and dATP can also act as donors. Also phosphorylates hydroxylamine and fluoride in the presence of CO2.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-59-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
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Manually annotated by BRENDA team
Amaranthus sp.
-
-
-
Manually annotated by BRENDA team
American eel, kinase resembles mammalian M2-isozyme
-
-
Manually annotated by BRENDA team
Antarctic fish
-
-
-
Manually annotated by BRENDA team
wild-type strain WG 096 (yA2, paba A1) and transformant 9/402
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-
Manually annotated by BRENDA team
A-5
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-
Manually annotated by BRENDA team
strain ATCC 19213
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Manually annotated by BRENDA team
strain ATCC 21616
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-
Manually annotated by BRENDA team
strain 168
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
Brassica juncea Pusa Bold
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-
Manually annotated by BRENDA team
Busycotypus canaliculatum
Cardium tuberculatum
cockle
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-
Manually annotated by BRENDA team
Loenberg, ice-fish
-
-
Manually annotated by BRENDA team
chickpea
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
strain ATCC 13032 lysCfbr
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-
Manually annotated by BRENDA team
sea bass, marine teleost
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-
Manually annotated by BRENDA team
strain PaPt36
SwissProt
Manually annotated by BRENDA team
strain PaPt36
SwissProt
Manually annotated by BRENDA team
rumen ciliates
-
-
Manually annotated by BRENDA team
strain JM101
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-
Manually annotated by BRENDA team
Escherichia coli K-12 W3110
isozymes PykA and PykF encoded by genes pykA and pykF
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-
Manually annotated by BRENDA team
strain K12 or B <45,60>
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-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
strain 9
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-
Manually annotated by BRENDA team
strain 9
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Manually annotated by BRENDA team
African land tortoise
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-
Manually annotated by BRENDA team
strain C10
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Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
lamprey
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-
Manually annotated by BRENDA team
nordic krill, isoforms PK I and PK II
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-
Manually annotated by BRENDA team
crustacean
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-
Manually annotated by BRENDA team
strain SN-G42 and 124A
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-
Manually annotated by BRENDA team
strain SN-G42 and 124A
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-
Manually annotated by BRENDA team
dimorphic phycomycete
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-
Manually annotated by BRENDA team
Musa cavendishii
banana
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-
Manually annotated by BRENDA team
strain CDC 46
-
-
Manually annotated by BRENDA team
strain CDC 46
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-
Manually annotated by BRENDA team
strain H37Rv
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-
Manually annotated by BRENDA team
strain H37Rv
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Manually annotated by BRENDA team
mussle
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Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
no activity in Clostridium thermocellum
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
chlorophyll-free mutant No. 20
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-
Manually annotated by BRENDA team
Alaskan king crab
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Manually annotated by BRENDA team
limpet
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-
Manually annotated by BRENDA team
cockroach
-
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Manually annotated by BRENDA team
Pigeon
-
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
Rhodosporidium toruloides
strain CBS 14
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Manually annotated by BRENDA team
Rhodosporidium toruloides CBS 14
strain CBS 14
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-
Manually annotated by BRENDA team
strain JT20454
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-
Manually annotated by BRENDA team
strain LT-2, highest activity in cells grown on glycolytic substrates, e.g. glucose, galactose, fructose or glycerol
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Manually annotated by BRENDA team
strain LT-2, highest activity in cells grown on glycolytic substrates, e.g. glucose, galactose, fructose or glycerol
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Manually annotated by BRENDA team
mollusc
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-
Manually annotated by BRENDA team
ruminal bacterium
Uniprot
Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
-
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-
Manually annotated by BRENDA team
a methicillin-resistant strain, gene pyk
UniProt
Manually annotated by BRENDA team
genes encoding pyruvate kinase and phosphofructokinase are cotranscribed and their expression is regulated at the transcriptional level in response to the sugars supplied
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Manually annotated by BRENDA team
strain JC2
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Manually annotated by BRENDA team
strain JC2
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
cyanobacterium, strain PCC 6301
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
archaebacterium
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Manually annotated by BRENDA team
strain K-100
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Manually annotated by BRENDA team
strain K-100
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Manually annotated by BRENDA team
mung bean
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Manually annotated by BRENDA team
dimorphic yeast
Uniprot
Manually annotated by BRENDA team
isozymes PykA and PykF encoded by genes pykA and pykF
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-
Manually annotated by BRENDA team
isozymes PykA and PykF encoded by genes pykA and pykF
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP + phosphoenolpyruvate
ATP + pyruvate
show the reaction diagram
ATP + prothymosin alpha
ADP + phospho-prothymosin alpha
show the reaction diagram
ATP + pyruvate
ADP + phosphoenolpyruvate
show the reaction diagram
CDP + phosphoenolpyruvate
CTP + pyruvate
show the reaction diagram
dADP + phosphoenolpyruvate
dATP + pyruvate
show the reaction diagram
dCDP + phosphoenolpyruvate
dCTP + pyruvate
show the reaction diagram
dGDP + phosphoenolpyruvate
dGTP + pyruvate
show the reaction diagram
dTDP + phosphoenolpyruvate
dTTP + pyruvate
show the reaction diagram
epsilon-ADP + phosphoenolpyruvate
?
show the reaction diagram
-
poor substrate
-
-
?
GDP + phosphoenolpyruvate
GTP + pyruvate
show the reaction diagram
IDP + phosphoenolpyruvate
ITP + pyruvate
show the reaction diagram
TDP + phosphoenolpyruvate
TTP + pyruvate
show the reaction diagram
-
poor substrate
-
-
?
UDP + phosphoenolpyruvate
UTP + pyruvate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ADP + phosphoenolpyruvate
ATP + pyruvate
show the reaction diagram
ATP + prothymosin alpha
ADP + phospho-prothymosin alpha
show the reaction diagram
ATP + pyruvate
ADP + phosphoenolpyruvate
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
UDP
-
activation
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cd2+
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multiphasical activation, at pH below physiological value, inhibits at physiological pH
Cu2+
-
divalent cation required, at 1 mM Cu2+ activation results in 86% of the activity with Mg2+
Divalent cation
Fe2+
-
the enzyme is dependent on Mn2+ ion for its activity. Fe2+ and Mg2+ show 70 and 20% of the Mn2+ activity, respectively. No activity is detected without metal or with Zn2+, Cu2+, Co2+, and Ni2+
Mg
-
required for activity
Monovalent cation
Ni2+
-
divalent cation required, at 1 mM Ni2+ activation results in 2% of the activity with Mg2+
sulfate
each monomer in the asymmetric unit of CpPyK binds two sulfate ions at equivalent positions, one in the C-domain and the other at the interface of the A and C domains, binding structure, overview. The sulfate ion in the C-domain occupies a position corresponding to the 6-phosphate of the effector molecule in different PyKs
Tl+
-
wild-type enzyme and the three mutant enzymes T298S, T298C and T298A show no measurable activity in the presence of K+ or Tl+. Tl+ can activate wild-type enzyme to 85% the activity in the presence of K+. With T298S, T298, and T298A, Tl+ is 1.2-1.8fold better activator than is K+ based on the measured turnover number values
additional information