Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.101 extracted from

  • Szumilo, T.
    A novel enzyme, tagatose kinase, from Mycobacterium butyricum (1981), Biochim. Biophys. Acta, 660, 366-370.
    View publication on PubMed
Search for more literature for 2.7.1.101

Inhibitors

Inhibitors Comment Organism Structure
CuSO4 1 mM, 50% inhibition Mycobacterium butyricum
HgCl2 2 mM, 32% inhibition Mycobacterium butyricum
MgCl2 above 3 mM Mycobacterium butyricum
additional information not inhibited by 1 mM N-ethylmaleimide, 1 mM iodoacetate, 1 mM NaF, 1 mM EDTA or 1 mM 1,10-phenanthroline Mycobacterium butyricum
p-chloromercuribenzoate 2 mM, 15% inhibition Mycobacterium butyricum
p-chloromercuriphenylsulfate 2 mM, 21% inhibition Mycobacterium butyricum
ZnSO4 1 mM, 38% inhibition Mycobacterium butyricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 D-tagatose at 22°C, pH 7.5 Mycobacterium butyricum
1
-
 ATP at 22°C, pH 7.5 Mycobacterium butyricum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 2.5 mM FeSO4, activates, can replace Mg2+ with 24% efficiency Mycobacterium butyricum
Mg2+ requirement, maximum activity in the presence of 3 mM MgCl2, inhibitory above 3 mM Mycobacterium butyricum
Mn2+ 2.5 mM MnCl2, activates, can replace Mg2+ with 63% efficiency Mycobacterium butyricum
additional information not activated by CaCl2, CoSO4, NiSO4, CdSO4 or ZnSO4 Mycobacterium butyricum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 gel filtration Mycobacterium butyricum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-tagatose Mycobacterium butyricum part of the dulcitol degradation pathway in saprophytic mycobacteria ADP + D-tagatose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium butyricum
-
 dulcitol-grown
-

Purification (Commentary)

Purification (Comment) Organism
106fold, partial Mycobacterium butyricum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.92
-
 at 37°C, pH 7.5 Mycobacterium butyricum

Storage Stability

Storage Stability Organism
room temperature, phosphate buffer, pH 6.3-8, 1 day, stable Mycobacterium butyricum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-tagatose highly specific for D-tagatose, phosphorylates D-tagatose at C-6 Mycobacterium butyricum ADP + D-tagatose 6-phosphate
-
 ir
ATP + D-tagatose part of the dulcitol degradation pathway in saprophytic mycobacteria Mycobacterium butyricum ADP + D-tagatose 6-phosphate
-
 ?
additional information no substrates: D-glucose, D-mannose, D-galactose, D-fructose, D-galactosamine, D-2-deoxygalactose, dulcitol, L-sorbose, L-rhamnose, D-ribose, D-xylose, D-lyxose, D-xylulose or glycerol Mycobacterium butyricum ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at, spectrophotometric method, measurement of ADP formation Mycobacterium butyricum
37
-
 assay at, measurement of the rate of disappearance of tagatose Mycobacterium butyricum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 1 min, stable, rapid inactivation above Mycobacterium butyricum
50
-
 rapidly loses activity on heating above Mycobacterium butyricum
56
-
 t1/2: 1 min Mycobacterium butyricum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 22°C Mycobacterium butyricum

pH Range

pH Minimum pH Maximum Comment Organism
6.4 8.4 about half-maximal activity at pH 6.4 and 8.4 Mycobacterium butyricum