Information on EC 2.7.1.101 - tagatose kinase

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The expected taxonomic range for this enzyme is: Mycobacterium

EC NUMBER
COMMENTARY
2.7.1.101
-
RECOMMENDED NAME
GeneOntology No.
tagatose kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + D-tagatose = ADP + D-tagatose 6-phosphate
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
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-
-
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PATHWAY
KEGG Link
MetaCyc Link
Galactose metabolism
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SYSTEMATIC NAME
IUBMB Comments
ATP:D-tagatose 6-phosphotransferase
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SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-tagatose 6-phosphate kinase
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-
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kinase, tagatose 6-phosphate (phosphorylating)
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tagatose 6-phosphate kinase (phosphorylating)
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tagatose-6-phosphate kinase
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CAS REGISTRY NUMBER
COMMENTARY
39434-00-9
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Mycobacterium butyricum
dulcitol-grown
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-tagatose
ADP + D-tagatose 6-phosphate
show the reaction diagram
Mycobacterium butyricum
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highly specific for D-tagatose, phosphorylates D-tagatose at C-6
-
ir
ATP + D-tagatose
ADP + D-tagatose 6-phosphate
show the reaction diagram
Mycobacterium butyricum
-
part of the dulcitol degradation pathway in saprophytic mycobacteria
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-
?
additional information
?
-
Mycobacterium butyricum
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no substrates: D-glucose, D-mannose, D-galactose, D-fructose, D-galactosamine, D-2-deoxygalactose, dulcitol, L-sorbose, L-rhamnose, D-ribose, D-xylose, D-lyxose, D-xylulose or glycerol
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + D-tagatose
ADP + D-tagatose 6-phosphate
show the reaction diagram
Mycobacterium butyricum
-
part of the dulcitol degradation pathway in saprophytic mycobacteria
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Fe2+
Mycobacterium butyricum
-
2.5 mM FeSO4, activates, can replace Mg2+ with 24% efficiency
Mg2+
Mycobacterium butyricum
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requirement, maximum activity in the presence of 3 mM MgCl2, inhibitory above 3 mM
Mn2+
Mycobacterium butyricum
-
2.5 mM MnCl2, activates, can replace Mg2+ with 63% efficiency
additional information
Mycobacterium butyricum
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not activated by CaCl2, CoSO4, NiSO4, CdSO4 or ZnSO4
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
CuSO4
Mycobacterium butyricum
-
1 mM, 50% inhibition
HgCl2
Mycobacterium butyricum
-
2 mM, 32% inhibition
p-chloromercuribenzoate
Mycobacterium butyricum
-
2 mM, 15% inhibition
p-Chloromercuriphenylsulfate
Mycobacterium butyricum
-
2 mM, 21% inhibition
ZnSO4
Mycobacterium butyricum
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1 mM, 38% inhibition
MgCl2
Mycobacterium butyricum
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above 3 mM
additional information
Mycobacterium butyricum
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not inhibited by 1 mM N-ethylmaleimide, 1 mM iodoacetate, 1 mM NaF, 1 mM EDTA or 1 mM 1,10-phenanthroline
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KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1
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ATP
Mycobacterium butyricum
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at 22C, pH 7.5
0.8
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D-tagatose
Mycobacterium butyricum
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at 22C, pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.92
-
Mycobacterium butyricum
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at 37C, pH 7.5
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.5
-
Mycobacterium butyricum
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22C
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.4
8.4
Mycobacterium butyricum
-
about half-maximal activity at pH 6.4 and 8.4
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
Mycobacterium butyricum
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assay at, spectrophotometric method, measurement of ADP formation
37
-
Mycobacterium butyricum
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assay at, measurement of the rate of disappearance of tagatose
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
63000
-
Mycobacterium butyricum
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gel filtration
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
48
-
Mycobacterium butyricum
-
1 min, stable, rapid inactivation above
50
-
Mycobacterium butyricum
-
rapidly loses activity on heating above
56
-
Mycobacterium butyricum
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t1/2: 1 min
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
room temperature, phosphate buffer, pH 6.3-8, 1 day, stable
Mycobacterium butyricum
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Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
106fold, partial
Mycobacterium butyricum
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