Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Streptomyces coelicolor |
expression in Escherichia coli | Streptomyces clavuligerus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | slight reduction of enzyme activity at high concentrations of acety-CoA | Streptomyces clavuligerus | |
acetyl-CoA | slight reduction of enzyme activity at high concentrations of acety-CoA | Streptomyces coelicolor | |
glyoxylate | slight reduction of enzyme activity at high concentrations of glyoxylate | Streptomyces clavuligerus | |
glyoxylate | slight reduction of enzyme activity at high concentrations of glyoxylate | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Streptomyces coelicolor | |
additional information | - |
additional information | - |
Streptomyces clavuligerus | |
0.00059 | - |
glyoxylate | - |
Streptomyces clavuligerus | |
0.00349 | - |
glyoxylate | - |
Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptomyces coelicolor | |
Mg2+ | required | Streptomyces clavuligerus | |
Mg2+ | optimal concentration 0.01 mM MgCl2 | Streptomyces clavuligerus | |
Mg2+ | optimal concentration 0.005 mM MgCl2 | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces clavuligerus | Q9ZH77 | NRRL3585 | - |
Streptomyces clavuligerus NRRL 3585 | Q9ZH77 | NRRL3585 | - |
Streptomyces coelicolor | Q9RKU9 | M130 | - |
Streptomyces coelicolor A3(2) | Q9RKU9 | M130 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzymes | Streptomyces coelicolor |
recombinant enzymes | Streptomyces clavuligerus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0012 | - |
- |
Streptomyces clavuligerus |
0.0262 | - |
- |
Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM | Streptomyces coelicolor | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM | Streptomyces clavuligerus | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM | Streptomyces coelicolor A3(2) | (S)-malate + CoA | - |
? | |
glyoxylate + acetyl-CoA + H2O | the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM | Streptomyces clavuligerus NRRL 3585 | (S)-malate + CoA | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 45 | - |
Streptomyces coelicolor |
20 | 45 | - |
Streptomyces clavuligerus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | significant reduction of specific activity at pH 7.0 and below | Streptomyces coelicolor |
8 | 10 | significant reduction of specific activity at pH 7.0 and below | Streptomyces clavuligerus |