Cloned (Comment) | Organism |
---|---|
gene GCN5B, DNA and amino acid sequence determination and analysis, real-time PCR enzyme expression analysis, recombinant expression of N-terminally HA-tagged enzyme TgGCN5b from parasites overexpressing an ectopic enzyme copy | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
garcinol | garcinaol inhibits TgGCN5b and mediates changes in the parasite transcriptome. Treatment of tachyzoites with garcinol leads to a reduction of global lysine acetylation, particularly on histone H3 and TgGCN5b itself | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Toxoplasma gondii | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone H3 | Toxoplasma gondii | preferred substrate | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3 | Toxoplasma gondii ME49 | preferred substrate | CoA + acetylhistone H3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | S8F3V2 | TgGCN5b | - |
Toxoplasma gondii ME49 | S8F3V2 | TgGCN5b | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally HA-tagged enzyme TgGCN5b from parasites overexpressing an ectopic enzyme copy by immunoaffinity chromatography | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + histone H3 | - |
Toxoplasma gondii | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3 | preferred substrate | Toxoplasma gondii | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3 | - |
Toxoplasma gondii ME49 | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3 | preferred substrate | Toxoplasma gondii ME49 | CoA + acetylhistone H3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCN5b | - |
Toxoplasma gondii |
histone acetyltransferase | - |
Toxoplasma gondii |
KAT | - |
Toxoplasma gondii |
lysine acetyltransferase | - |
Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Toxoplasma gondii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
malfunction | the dysregulated gene expression induced by garcinol inhibition of the enzyme significantly inhibits Toxoplasma tachyzoite replication without being toxic to the human host cell. Garcinol inhibits TgGCN5b KAT activity and reduces global lysine acetylation in vivo in treated parasites, including its preferred substrate, histone H3 | Toxoplasma gondii |
physiological function | lysine acetylation is a critical post-translational modification that influences protein activity, stability, and binding properties. The acetylation of histone proteins in particular is a feature of gene expression regulation. TgGCN5b is the only nuclear GCN5-family KAT known to be required for Toxoplasma tachyzoite replication | Toxoplasma gondii |