Information on EC 2.3.1.48 - histone acetyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
2.3.1.48
-
RECOMMENDED NAME
GeneOntology No.
histone acetyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
ping-pong mechanism
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
A group of enzymes with differing specificities towards histone acceptors
-
-
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
ping-pong mechanism, enzyme form NII
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
acetylation of lysine 5, 8, 12, and 16 of free histone H4
Q8WYB5
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
acetylation of lysine 5, 8, 12, and 16 of free histone H4
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
acetylation of lysine 5, 8, 12, and 16 of free histone H4
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
acetylation of lysine 5, 8, 12, and 16 of free histone H4
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
rapid equilibrium ordered bireactant mechanism
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
mechanisms
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
mechanisms
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
mechanisms
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
MORF protein is also active as transcription factor
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
ternary complexed mechanism for PCAF and Gnc5
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
ternary complexed mechanism for PCAF and Gnc5
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
enzymes are also active as transcription coactivators and corepressors of transcription factors in gene regulation, acetylation of transcription factors, overview
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
PCAF and Gcn5 protein, fully ordered Bi-Bi kinetic mechanism with acetyl-CoA binding before histone H3
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
PCAF and Gcn5 protein, fully ordered Bi-Bi kinetic mechanism with acetyl-CoA binding before histone H3
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
binding motifs
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
ordered sequential catalytic mechanism of the MYST HAT with a direct-attack mechanism and direct acetyl transfer mechanism, not mediated by Cys304, within an Esa1acetyl-CoAhistone ternary complex, acetyl-CoA binds first and CoA is the last product released
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
conserved catalytic mechanisms of HATs, overview. p300 utilizes a special type of sequential mechanism with a short-lived ternary complex, known as a Theorell-Chance mechanism
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
conserved catalytic mechanisms of HATs, overview. p300 utilizes a special type of sequential mechanism with a short-lived ternary complex, known as a Theorell-Chance mechanism. For Gcn5, p/CAF, and p300, only 3 to 5 residues on either side of the substrate lysine are required for efficient binding and catalysis by the catalytic domain
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
non-processive mechanism, where the NuA4 histone acetyltransferasecomplex dissociates from substrate after each acetylation event
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
sequential mechanism in which acetyl-CoA and H3 bind to a complex of isoform Rtt109 and histone chaperone Vps75 without obligate order, followed by the direct attack of the unprotonated epsilon-amino group on acetyl-CoA, transferring the acetyl-group to H3 lysine residues The chemical attack of substrate lysine on the bound acetyl-CoA is the rate-limiting step of catalysis
Q07794
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
enzymes are also active as transcription coactivators and corepressors of transcription factors in gene regulation, acetylation of transcription factors, overview, mechanisms, binding motifs
Homo sapiens GCN5
-
-
acetyl-CoA + histone = CoA + acetylhistone
show the reaction diagram
mechanisms, binding motifs
Tetrahymena thermophila GCN5
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:histone acetyltransferase
A group of enzymes with differing specificities towards histone acceptors.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
acetyltransferase, histone
-
-
-
-
histone acetokinase
-
-
-
-
histone acetylase
-
-
-
-
histone transacetylase
-
-
-
-
nucleosome-histone acetyltransferase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
9054-51-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Columbia-0 ecotype
-
-
Manually annotated by BRENDA team
ecotype Wassilewskija, genes gcn5-1 and gcn5-2
-
-
Manually annotated by BRENDA team
genes ham1 and ham2
-
-
Manually annotated by BRENDA team
HAM1; HAM1 or HAG4
UniProt
Manually annotated by BRENDA team
HAM2; HAM2 or HAG5
UniProt
Manually annotated by BRENDA team
Artemia nauplii
3 enzyme forms: I, II and III
-
-
Manually annotated by BRENDA team
2 enzyme forms: A and B; calf
-
-
Manually annotated by BRENDA team
3 enzyme forms: A, B, C; calf
-
-
Manually annotated by BRENDA team
enzyme forms A, B and DB
-
-
Manually annotated by BRENDA team
clinical isolate SC5314, ORF19.7491
-
-
Manually annotated by BRENDA team
zMoz
-
-
Manually annotated by BRENDA team
Danio rerio zMoz
zMoz
-
-
Manually annotated by BRENDA team
HBO1, Mof, Enok, and chameau
-
-
Manually annotated by BRENDA team
Herpes simplex virus
-
-
-
Manually annotated by BRENDA team
ATAC2
UniProt
Manually annotated by BRENDA team
catalytic subunit of the male-specific lethal hiostone acetyltransferase complex
UniProt
Manually annotated by BRENDA team
fragment EST clone of MORF protein, i.e. monocytic leukemia zinc finger protein-related factor
SwissProt
Manually annotated by BRENDA team
GCN5
-
-
Manually annotated by BRENDA team
GCN5; PCAF protein
-
-
Manually annotated by BRENDA team
GCN5; recombinant enzyme hPCAF, i.e. human p300/CBP associated factor
-
-
Manually annotated by BRENDA team
gene Hbo1
-
-
Manually annotated by BRENDA team
gene taf1
-
-
Manually annotated by BRENDA team
MORF protein, i.e. monocytic leukemia zinc finger protein-related factor
-
-
Manually annotated by BRENDA team
PCAF protein
-
-
Manually annotated by BRENDA team
Tip60, p/CAF, and p300
-
-
Manually annotated by BRENDA team
Homo sapiens GCN5
GCN5
-
-
Manually annotated by BRENDA team
i.e. HIV; recombinant from COS cell line and from Escherichia coli
-
-
Manually annotated by BRENDA team
C57BL/6 mice
-
-
Manually annotated by BRENDA team
gene cbp
-
-
Manually annotated by BRENDA team
gene Gcn5
-
-
Manually annotated by BRENDA team
MNof, Tip60, Qkf, Moz, and Morf
-
-
Manually annotated by BRENDA team
PCAF and CBP
-
-
Manually annotated by BRENDA team
Swiss albino mice, 2 months old
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6
C57BL/6 mice
-
-
Manually annotated by BRENDA team
3 enzyme forms: A1, A2, B
-
-
Manually annotated by BRENDA team
2 enzyme forms: A and B
-
-
Manually annotated by BRENDA team
cv. Lincoln; enzyme form B
-
-
Manually annotated by BRENDA team
enzyme form B
-
-
Manually annotated by BRENDA team
gene PF11_0192, putative
UniProt
Manually annotated by BRENDA team
2 enzyme forms NI and NII from nucleus and 2 enzyme forms CI and CII from cytoplasm
-
-
Manually annotated by BRENDA team
2 enzyme forms: A and B
-
-
Manually annotated by BRENDA team
3 enzyme forms: A, B1, B2
-
-
Manually annotated by BRENDA team
male sprague-dawley rats
-
-
Manually annotated by BRENDA team
diverse strains
-
-
Manually annotated by BRENDA team
enzyme form A and B
-
-
Manually annotated by BRENDA team
genes/proteins Sas2, Sas4, Sas5 forming the SAS complex
-
-
Manually annotated by BRENDA team
nucleosomal H2A/H4 acetyltransferase, i.e. NuA4 protein
-
-
Manually annotated by BRENDA team
p300, Gcn5, Rtt109, and Esa1
-
-
Manually annotated by BRENDA team
several strains
-
-
Manually annotated by BRENDA team
several strains, overview, gene HAT1
-
-
Manually annotated by BRENDA team
strain BY4705
-
-
Manually annotated by BRENDA team
wild-type strain BY4741 and deletion derivatives
-
-
Manually annotated by BRENDA team
wild-type strain W303-1a, gene hat1
-
-
Manually annotated by BRENDA team
yeast piccolo NuA4 complex
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae BY4705
strain BY4705
-
-
Manually annotated by BRENDA team
diverse strains, overview
-
-
Manually annotated by BRENDA team
several strains, overview, gene HAT1
Uniprot
Manually annotated by BRENDA team
from infected african green monkey kidney cell line CV-1; i.e. SV40
-
-
Manually annotated by BRENDA team
enzyme form type I and type II
-
-
Manually annotated by BRENDA team
protein p55
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
Tetrahymena sp. GCN5
GCN5
-
-
Manually annotated by BRENDA team
transcription-associated enzyme form A, containing protein p55 as catalytic subunit; yeast-NuA4-like enzyme form
-
-
Manually annotated by BRENDA team
Tetrahymena thermophila GCN5
GCN5
-
-
Manually annotated by BRENDA team
enzyme form B
Uniprot
Manually annotated by BRENDA team
enzyme form B; strain Cuzco
-
-
Manually annotated by BRENDA team
Zea mays Cuzco
strain Cuzco
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
cells lacking RTT109 have a high level of CAG/CTG repeat contractions and a twofold increase in breakage at CAG/CTG repeats
malfunction
Q9H7Z6
disturbance of normal acetylation of K16 in histone H4 together with trimethylation of Lys20 in histone H4 is associated with early stages of tumor development
malfunction
-
dysfunction is associated with diseases like asthma, cardiovascular disorders, diabetes, and cancer
malfunction
-
dysfunction of histone acetyltransferases leads to several diseases including cancer, diabetes, and asthma
malfunction
-
H4K16 hyperacetylation is associated with hyperexpression of the single male X chromosome in flies and, contrasting accordingly, the inactivated X chromosome in human cells is hypoacetylated at the same histone residue. HBO1 appears to function predominantly in transcriptional repression
malfunction
-
HBO1 depletion reduces the rate of DNA synthesis, the amount of MCM complex bound to chromatin, and progression through S phase
malfunction
-
MOZ generates fusion genes, such as MOZ-TIF2, MOZ-CBP and MOZ-p300, in acute myeloid leukemia by chromosomal translocation leading to repressed differentiation, hyper-proliferation, and self-renewal of myeloid progenitors through deregulation of MOZ-regulated target gene expression. Roles of MOZ and MOZ fusion genes in normal and malignant hematopoiesis, mechanism, overview
malfunction
-
the haploinsufficient enzyme causes the Rubinstein-Taybi syndrome, a genetic disorder with cognitive dysfunction, by disrupting the control mechanism of neural precursor competency to differentiate
malfunction
-
expression of mutant TgMYST-B produces no growth defect and fails to protect against DNA damage, overexpression of recombinant, tagged TgMYST-B reduces growth rate in vitro and confers protection from a DNA-alkylating agent. Cells overexpressing TgMYST-B have increased levels of ataxia telangiectasia mutated (ATM) kinase and phosphorylated H2AX and that TgMYST-B localizes to the ATM kinase gene. Pharmacological inhibitors of ATM kinase or KATs reverse the slow growth phenotype seen in parasites overexpressing TgMYST-B
malfunction
Saccharomyces cerevisiae BY4705
-
cells lacking RTT109 have a high level of CAG/CTG repeat contractions and a twofold increase in breakage at CAG/CTG repeats
-
metabolism
-
beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1) is acetylated in seven lysine residues that face the lumen of the ER and ER Golgi intermediate compartment (ERGIC)
metabolism
-
histone and non-histone protein acetylation is involved, directly and indirectly, in controlling the duration, strength and specificity of the NF-kappaB-activating signaling pathway at multiple levels. Overview of the NF-kB-signaling pathway. Different signaling pathways can interfere with one another by modulating the availability of HATs or HDACs for a particular transcription complex
metabolism
-
high mobility group domain-containing protein And-1 overexpression stabilizes Gcn5 through protein-protein interactions in vivo
metabolism
-
the ablation of Cullin4-RING E3 ubiquitin ligase CRL4 leads to the stabilization of isoform Gcn5 in cells with depleted And-1, and Cdc10-dependent transcript 2, i.e. Cdt2, serves as a substrate receptor protein of CRL4. Overexpression of Cdt2 reduces the isoform Gcn5 protein levels, and CRLCdt2 is sufficient to ubiquitinate Gcn5 both in vivo and in vitro. And-1 stabilizes Gcn5 by impairing the interaction between Gcn5 and CRLCdt2 and thereby preventing Gcn5 ubiquitination and degradation. The degradation of Gcn5 is not dependent on proliferating cell nuclear antigen
physiological function
-
ATAC2 not only carries out an enzymatic function but also plays an architectural role in the stability of mammalian ATAC
physiological function
Q9H8E8
ATAC2 not only carries out an enzymatic function but also plays an architectural role in the stability of mammalian ATAC
physiological function
-
CBP regulates neurobehavioural development, the enzyme activity and histone acetylation is required for control of neural cortical precursor competency to differentiate, regulation via environmental factors
physiological function
-
Esa1 catalytic HAT activity is essential in yeast binding acetyl-CoA or lysine substrates and positively regulating the activities of NuA4 and Piccolo NuA4, Esa1 is involved in DNA damage repair
physiological function
-
Esa1 mediates increased H4 acetylation and enhanced chromatin remodeling complex RSC occupancy and histone eviction in coding sequences and stimulates the rate of transcription elongation by polymerase II
physiological function
-
GCN5 has a general repressive effect on microRNAs, miRNAs, that guide sequence-specific posttranscriptional gene silencing, but is required for expression of a subset of MIRNA genes, overview
physiological function
-
genome-wide increase in histone acetylation stimulates replication independently of transcription in follicle cells. Enok is essential for mushroom body development, the mushroom bodies are the sites of olfactory learning and memory and in this function equivalent to the mammalian brain. Mof is required for sex chromosome dosage compensation acting in the MSL complex
physiological function
-
HBO1 histone acetylase is important for DNA replication licensing in a Cdt1-dependent manner, overview. HBO1 plays a direct role at replication origins as a coactivator of the Cdt1 licensing factor. As HBO1 is also a transcriptional coactivator, it has the potential to integrate internal and external stimuli to coordinate transcriptional responses with initiation of DNA replication. HBO1 is not required for Cdt1 association with replication origins
physiological function
-
Hbo1 plays a role as chromatin factor serving as a positive regulator of DNA replication , chromatin structure plays an important role in DNA replication initiation
physiological function
-
histone acetylation by the enzyme plays an integral role in the epigenetic regulation of gene expression
physiological function
-
histone acetylation is one of the major epigenetic mechanisms to regulate gene expression. MOZ is essential for the generation and maintenance of hematopoietic stem cells and for the appropriate development of myeloid, erythroid and B-lineage cell progenitors. MOZ is also required for self-renewal of hematopoietic stem cells
physiological function
-
histone acetyltransferases and deacetylases play critical roles in the regulation of chromatin structure and gene expression
physiological function
-
histone and non-histone protein acetylation is involved, directly and indirectly, in controlling the duration, strength and specificity of the NF-kappaB-activating signaling pathway at multiple levels. Overview of the NF-kB-signaling pathway, IkappaBalpha and some members of the IKK complex have a nuclear function involving HAT and HDAC recruitment
physiological function
-
intrinsic HAT activity of p300 plays an important role in the transcriptional coactivation of CREB, c-Jun, c-Fos, c-Myb, p53, Stats, nuclear receptors, RelA GATA, p73, and others. The enzyme is involved in post-translational modifications of chromatin that play a key role in the regulation of gene expression, cell growth, and differentiation
physiological function
-
Mst1 is essentially required for damage response and chromosome segregation, it plays essential roles in maintenance of genome stability and recovery from DNA damage
physiological function
-
MYST1 plays a role in tumor suppression mechanisms, functional composition and mechanisms of MYST1-containing complexes, overviewS
physiological function
Q9H7Z6
MYST1 plays a role in tumor suppression mechanisms, functional composition and mechanisms of MYST1-containing complexes, overviewS
physiological function
-
p300 plays a key role in NFkappaB subunit acetylation
physiological function
-
Qkf/Morf requirement in neural stem cell/neural progenitor self-renewal with an additional role in some other cell types such as osteoblasts and germ cells. Qkf in adult neurogenesis in vivo, overview
physiological function
-
role for Rtt109 and H3K56 acetylation in maintaining repetitive DNA sequences in Saccharomyces cerevisiae
physiological function
-
Rtt109 is important for repairing replication-associated lesions and has functions in addition to maintaining genome stability
physiological function
-
Rtt109 is important for yeast model organisms to survive DNA damage and maintain genome integrity, and Rtt109 is particularly important for fungal pathogenicity
physiological function
-
Sas2 is required for subtelomeric reporter transgene silencing, but also for transcriptional activity of transgenes integrated into rDNA, for transcriptional activation of a mutated HMRE silent mating type locus and for protection of euchromatin from heterochromatin spreading
physiological function
-
the enzyme activity of MOZ is critical for the proliferation of hematopoietic precursors, overview
physiological function
-
the SAGA complex contains the histone ubiquitin protease Ubp8 and the histone acetyltransferase Gcn5 and is responsible for efficient transcription of SAGA regulated genes such as GAL1 and ADH2
physiological function
-
Tip60 plays multiple roles in chromatin remodeling processes. Tip60 is a partner of the epigenetic integration platform interplayed by UHRF1, DNMT1 and HDAC1 involved in the epigenetic code replication
physiological function
-
a histone modifying complex, composed of the Lsy-12 MYST-type histone acetyltransferase, the Ing-family plant homeodomain protein Lsy-13, and plant homeodomain/bromodomain protein Lin-49, is required to first initiate and then actively maintain lateralized gene expression in the gustatory system. A combination of transcription factors, which recognize DNA in a sequence-specific manner, and a histone modifying enzyme complex are responsible for inducing and maintaining neuronal laterality. In lsy-12 mutants the normally ASER-specific gcy-5 gene is expressed bilaterally from the onset of its expression in threefold embryos
physiological function
-
co-expression of histone acetyltransferase E1A binding protein p300 dramatically enhances Pax5-mediated transcriptional activation. The p300-mediated enhancement is dependent on its intrinsic histone acetyltransferase activity. Moreover, p300 interacts with the C terminus of Pax5 and acetylates multiple lysine residues within the paired box DNA-binding domain of Pax5. Mutations of lysine residues 67 and 87/89 to alanine within Pax5 abolish p300-mediated enhancement of Pax5-induced Luc-CD19 reporter expression in HEK-293 cells
physiological function
-
coexpressing isoform TIP60 decreases the transcriptional activation ability of c-Myb in functional reporter assays. TIP60 binds to the c-Myb target gene c-Myc promoter in a c-Myb-dependent manner. Knockdown of isoform Tip60 expression by siRNA increases endogenous c-Myc expression. c-Myb is associated with histone deacetylases HDAC1 and HDAC2, known to interact with TIP60 and repress transcription
physiological function
Q8III2
enzyme is essential for asexual intraerythrocytic growth. Overexpression of the long, active or a truncated, non-active version of the protein by stable integration of the expression cassette in the parasite genome results in changes of H4 acetylation and cell cycle progression. Overexpressing parasites shows changes in sensitivity to DNA-damaging agents
physiological function
-
high mobility group domain-containing protein And-1 forms a complex with both histone H3 and isoform Gcn5. Downregulation of And-1 results in Gcn5 degradation, leading to the reduction of histone H3K9 and H3K56 acetylation. And-1 overexpression stabilizes Gcn5 through protein-protein interactions in vivo. And-1 expression is increased in cancer cells in a manner correlating with increase in Gcn5 and acetylation of H3K9 and H3K56
physiological function
-
histone acetyltransferase CLOCK is a component of the transcriptional complex that includes transcriptional factor TFIID, and infected cell proteins ICP4, ICP27, and ICP22. CLOCK histone acetyltransferase is a component of the viral transcriptional machinery throughout the replicative cycle of the virus and ICP27 and ICP22 initiate their involvement in viral gene expression as components of viral transcriptome
physiological function
-
histone acetyltransferase Mof plays an essential role in the maintenance of embryonic stem cell self-renewal and pluripotency. Embryonic stem cells with Mof deletion lose characteristic morphology, alkaline phosphatase staining, and differentiation potential. They also have aberrant expression of the core transcription factors Nanog, Oct4, and Sox2. The phenotypes of Mof null embryonic stem cells can be partially suppressed by Nanog overexpression, supporting the idea that Mof functions as an upstream regulator of Nanog in embryonic stem cells. Mof is an integral component of the embryonic stem cell core transcriptional network and Mof primes genes for diverse developmental programs. Mof is also required for Wdr5 recruitment and histone H3K4 methylation at key regulatory loci
physiological function
F4IXE7
isoform IDM1is a regulator of DNA demethylation. IDM1 is required for preventing DNA hypermethylation of highly homologous multicopy genes and other repetitive sequences that are normally targeted for active DNA demethylation by Repressor of Silencing 1 and related 5-methylcytosine DNA glycosylases. IDM1 binds methylated DNA at chromatin sites lacking histone H3K4 di- or trimethylation and acetylates H3 to create a chromatin environment permissible for 5-methylcytosine DNA glycosylases to function
physiological function
-
MYST protein acetyltransferase activity requires active site lysine autoacetylation
physiological function
Q9AR19
plants homozygous for T-DNA disruption alleles of GCN5 encoding a histone acetyltransferase show diminished expression of cold-regulated genes COR during cold acclimation. H3 acetylation at COR gene promoters is stimulated upon cold acclimation in gcn5 plants as in wild type plants, but the decrease in nucleosome occupancy is diminished. Thus, GCN5 is not the enzyme responsible for histone acetylation at COR gene promoters during cold acclimation
physiological function
Q93ZR1
protein mediates establishment of leaf polarity independently of ASYMMETRIC LEAVES2 and the trans-acting small interfering RNA-related pathway. Treatment with an inhibitor of histone deacetylases causes additive polarity defects in as2-1 east1-1 mutant plants. Isoform ELO3 may be involved, independent of the HDAC pathway, in the determination of polarity
physiological function
Q9VQZ6
targeted reduction of ELP3 specifically in the developing Drosophila nervous system leads to a hyperactive phenotype with increase in climbing and locomotor activities and sleep loss in the adult flies, a significant expansion in synaptic bouton number and axonal length and branching in the larval neuromuscular junction as well as the misregulation of genes involved in sleep, vesicle transport and fusion, and protein chaperone activity. Ubiquitous reduction of ELP3 results in lethality
physiological function
-
transcriptional coactivator gcn5 gene replacement mutants show a mild growth deficiency. Gcn5 is required for adaptation to stresses mediated by KCl and CaCl2, calcoflour white, MnCl2 and caffeine. The histone acetyltransferase activity of Gcn5 is required for its role in stress response
physiological function
-
transcriptional coactivytor gcn5 gene replacement mutants show a mild growth deficiency. Gcn5 is required for adaptation to stresses mediated by KCl and CaCl2, calcoflour white, MnCl2 and caffeine. The histone acetyltransferase activity of Gcn5 is required for its role in stress response. Gcn5-dependent KCl response genes include membrane transporter VMR1 and heat-shock-response gene SSA4. The FLO8 gene, which encodes a transcriptional regulator, is up-regulated in the mutant. During KCl stress adaptation, Gcn5 shows a tendency for redistribution from short genes to the transcribed regions of long genes
physiological function
Saccharomyces cerevisiae BY4705
-
role for Rtt109 and H3K56 acetylation in maintaining repetitive DNA sequences in Saccharomyces cerevisiae
-
physiological function
Mus musculus C57BL/6
-
the enzyme activity of MOZ is critical for the proliferation of hematopoietic precursors, overview
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4 acetyl-CoA + histone H4
4 CoA + tetraacetylhistone H4
show the reaction diagram
-
-
NuA4 randomly acetylates free and nucleosomal H4, with a small preference for lysines 5, 8, and 12 over 16
-
?
acetyl-CoA + 1,4-butanediamine
?
show the reaction diagram
-
enzyme form A and B, low activity, i.e. putrescine
-
-
?
acetyl-CoA + 1,5-pentanediamine
?
show the reaction diagram
-
enzyme form A and B, low activity, i.e. cadaverine
-
-
?
acetyl-CoA + 1,6-hexanediamine
?
show the reaction diagram
-
enzyme form A shows low activity , B not
-
-
?
acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1
CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1
show the reaction diagram
-
50 mM Tris-HCl, pH 8.0, 30C
-
-
?
acetyl-CoA + c-Myc
CoA + acetylated c-Myc
show the reaction diagram
-
acetylation by Tip60 increases c-Myc protein stability in transfected H-1299 human lung carcinoma cells, acetylation by Tip60
-
-
?
acetyl-CoA + H3 peptide
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
ir
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
-
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Q8LPU4
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
-
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
enzyme form B nearly exclusively acetylates histones H4 and H2a
formation of N6-acetyllysine as the only acetylation product
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Q8WYB5
calf thymus and HeLa cell histones
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
pea histones, enzyme form A and B
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone H2A is a substrate for enzyme form A1
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
free histones
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
free histones
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
chicken erythrocyte histones, enzyme form A and B
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
chicken erythrocyte histones, enzyme form A and B
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
nucleosome-histones, enzyme form A, not enzyme form B
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone f1, enzyme form A, histone f2a1, enzyme form B1 and B2
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
nucleosome-histones, enzyme form A and B
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Q8WYB5
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Artemia nauplii
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
acetylates histones H2A, H3, and H4, but not histone 2B
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
free pea and chicken histones H4, enzyme form B
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
or chymotryptic peptides of histone
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
chymostatic peptides of histones
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone H1 is a better substrate than H3 or H4
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone H1 poor substrate
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone H2B: poor substrate for enzyme form A2
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
acetylation of lysine 5, 8, 12, and 16 of free histone H4 with increasing preference
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone H1 is not acetylated in vivo
-
-
-
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
Esa1 protein is involved in cell cycle regulation
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
involved in chromatin remodeling and DNA repair
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone H3 is the preferred substrate
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
neutralization of positively charged lysine residues by acetylation lowering the affinity of histone octamers for the negatively charged DNA
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
involved in dynamic equilibrium of core histone acetylation
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
most likely involved in acetylation of newly synthesized histones in cytoplasm prior to chromatin assembly
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
important role of the enzyme for chromatin modulating activity
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Q9EQQ9
the bifunctional enzyme NCOAT, nuclear cytoplasmic O-GlcNacase and acetyltransferase, may be regulated to reduce the state of glycosylation of transcriptional activators while increasing the acetylation of histones to allow for concerted activation of eukaryotic gene transcription
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone acetylation on Lys16 by Sas2
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
histone H1 is not acetylated in vivo
-
-
-
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
a group of enzymes with differing specificity towards histone acceptors, specificity of different enzyme forms, neutralization of positively charged lysine residues by acetylation lowering the affinity of histone octamers for the negatively charged DNA, the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
-
-
ir
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
involved in chromatin remodeling and DNA repair, histone H3 is the preferred substrate
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Tetrahymena thermophila GCN5
-
-
-
-
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Tetrahymena thermophila GCN5
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Zea mays Cuzco
-
-
-
?
acetyl-CoA + histone H
CoA + acetylhistone H
show the reaction diagram
-
histone acetyltransferase AtGCN5 is required to regulate the floral meristem activity through the WUS/AG pathway
-
-
?
acetyl-CoA + histone H1
CoA + acetylhistone H1
show the reaction diagram
-
histone H1 is a better substrate than H3 or H4
-
-
?
acetyl-CoA + histone H1
CoA + acetylhistone H1
show the reaction diagram
-
histone H1 poor substrate
-
-
?
acetyl-CoA + histone H1
CoA + acetylhistone H1
show the reaction diagram
-
histone H1 poor substrate
-
-
?
acetyl-CoA + histone H1
CoA + acetylhistone H1
show the reaction diagram
-
acetylation of histone H1 only in vitro
-
-
?
acetyl-CoA + histone H1
CoA + acetylhistone H1
show the reaction diagram
Homo sapiens GCN5
-
histone H1 poor substrate, acetylation of histone H1 only in vitro
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
-
-
-
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
-
-
-
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
-
-
-
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
Q9H7Z6
-
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
enzyme form B nearly exclusively acetylates histones H4 and H2a
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
histone H2A is a substrate for enzyme form A1
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
NuA4-like protein acetylates histone H4 and H2A
-
-
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
NuA4-like protein acetylates histone H4 and H2A
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
MYST-related histone acetyltransferase complex Tip60
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
acetylation at Lys5 by Tip60
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
Mus musculus C57BL/6
-
-
-
-
?
acetyl-CoA + histone H2A
Nalpha-acetylated-histone H2A + CoA
show the reaction diagram
Q04751
-
-
-
?
acetyl-CoA + histone H2B
CoA + acetylhistone H2B
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H2B
CoA + acetylhistone H2B
show the reaction diagram
-
histone H2B: poor substrate for enzyme form A2
-
?
acetyl-CoA + histone H2B
CoA + acetylhistone H2B
show the reaction diagram
-
histone H2B: preferred substrate of enzyme form A
-
?
acetyl-CoA + histone H2B
CoA + acetylhistone H2B
show the reaction diagram
-
GNAT-related histone acetyltransferase complexes SAGA, ADA or H2B
-
-
?
acetyl-CoA + histone H2B
CoA + acetylhistone H2B
show the reaction diagram
-
GNAT-related histone acetyltransferase complexes STAGA or TFTC
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
r
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Q8WYB5
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Q92831
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Q9H7Z6
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Q07794
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
F4IXE7
-
residues K14, K18, and K23 of H3 are acetylated by domain C1 of isoform Idm1 in vitro
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
histone H1 is a better substrate than H3 or H4
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
PCAF protein
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Gcn5 protein: specific for Lys14 of histone H3
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Gcn5 protein: preferred substrate, acetylation at Lys14
-
r
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
histone H3: preferred substrate
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
histone H3: preferred substrate
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
histone H3: preferred substrate
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
enzyme form B, very low activity
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
histone H3 preferred substrate of enzyme form A
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
histone H3 is the preferred substrate of enzyme form A1 and A2
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
recombinant and native SAS complex acetylates Lys14
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
GNAT-related histone acetyltransferase complexes PCAF, STAGA or TFTC
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
GNAT-related histone acetyltransferase complexes SAGA, ADA or HAT-A2, MYST-related histone acetyltransferase complex NuA3
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
strong preference for free histones relative to chromatin substrate
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys14 by tGCN5 in the consensus sequence QTARKSTGGK14APRKLASK
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
CBP binds and acetylates histones at neural promoters, and regulates Corpus Callosum development. CBP binds to neuronal and glial promoters and globally promotes histone acetylation in the embryonic cortex, e.g. the beta-actin promoter, overview
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
H4R3 methylation, catalyzed by PRMT1, facilitates beta-globin transcription by regulating histone acetyltransferase binding, and histone H3 and H4 acetylation, overview
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
regulation, detailed overview. Acetylation and deacetylation events, in combination with other post-translational protein modifications, generate an NF-kappaB-signaling code and regulate NF-kappaB-dependent gene transcription in an inducer- and promoter-dependent manner, overview
-
-
r
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Rtt109 association with distinct histone chaperones directs substrate selection between N-terminal lysines, H3K9, H3K23, and those within the histone fold domain, H3K56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Rtt109 is specific for histone H3, acetylation at Lys9 and Lys56. RTT109 has functions in addition to maintaining genome stability
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation at Lys14 of histone H3
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys14 by tGCN5 in the consensus sequence QTARKSTGGK14APRKLASK. Phe125 and Phe164 interact with the substrate, but are not directly involved in the acetylation reaction, while residues Glu122, Val123 and Tyr160 are critical for catalysis
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys9 and Lys14
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys9 and Lys14 by PCAF. PCAF binds to dimethyl-Arg3 at histone H4 tails, dimethyl H4R3 provides a binding surface for PCAF and directly enhances histone H3 and H4 acetylation in vitro
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Rtt109 association with distinct histone chaperones directs substrate selection between N-terminal lysines, H3K9, H3K23, and those within the histone fold domain, H3K56. The sequence G-K-X-P within histone H3, which includes the primary Gcn5 substrate K14, makes several key contacts within the active site that are conserved with other GNAT members
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Rtt109 is specific for histone H3, acetylation at Lys9 and Lys56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
substrate is a H3 peptide
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
the sequence G-K-X-P within histone H3 makes several key contacts within the active site that are conserved in GNAT members including p/CAF
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
the sequence G-K-X-P within histone H3, which includes the primary Gcn5 substrate K14, makes several key contacts within the active site that are conserved in GNAT members
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Saccharomyces cerevisiae BY4705
-
acetylation of Lys56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Homo sapiens GCN5
-
PCAF protein
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Tetrahymena sp. GCN5
-
the sequence G-K-X-P within histone H3, which includes the primary Gcn5 substrate K14, makes several key contacts within the active site that are conserved in GNAT members
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Zea mays Cuzco
-
enzyme form B, very low activity
-
?
acetyl-CoA + histone H3
peptide CoA + acetylhistone H3 peptide
show the reaction diagram
-
preferred substrate, the N-terminal substrate region plays an importsant role in enhanced affinity of the Gcn5/PCAF proteins for histone H3
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone
show the reaction diagram
Q09472
-
-
-
?
acetyl-CoA + histone H3 N-terminal tail
CoA + acetylated histone H3 N-terminal tail
show the reaction diagram
-
50 mM Tris-HCl, pH 8.0, 30C
-
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF
-
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
peptide H3p20
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
peptide H3p20
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
peptide H3p20
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
-
peptide H3p20
-
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
Homo sapiens GCN5
-
peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF, peptide H3p20
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
Homo sapiens GCN5
-
peptide H3p20
-
?
acetyl-CoA + histone H3 peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
Tetrahymena thermophila GCN5
-
peptides H3p19, H3p27, H3p11 are substrates for the catalytic domain of Gcn5 and PCAF, peptide H3p20
-
-
?
acetyl-CoA + histone H3 peptide
CoA + actylhistone H3
show the reaction diagram
-
residues 1-21 of human histone H3
-
-
-
acetyl-CoA + histone H3 tail peptide
CoA + acetylhistone H3 peptide
show the reaction diagram
Q07794
H3 peptide substrate, amino acid sequence ARTKQTARKSTGGKAPRKQL
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
r
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q9H7Z6
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q8III2
-
acetylates histone H4 in vitro at K5, K8, K12 and K16
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
highly specific for
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
highly specific for
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
enzyme form B nearly exclusively acetylates histones H4 and H2a
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
Gcn5 protein acetylates H4 when purified and presented separately to the enzyme at Lys8 and Lys16
-
r
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
NuA4-like protein acetylates histone H4 and H2A
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
SAS complex, native and recombinant, acetylates Lys16
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
histone H4: all of the acetate groups are introduced within the NH2-terminal amino acids 4 to 17
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
enzyme form B has a marked specificity for histone H4
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
enzyme form B
mono-, di- and triacetylated products
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
enzyme form B
mono- and diacetylated products
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
enzyme form B is specific for histone H4
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
enzyme form B is specific for histone H4
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
histone H4 is a poor substrate
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
PCAF protein
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
histone H4 is the preferred substrate
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q8WYB5
histone H4 is the preferred substrate
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
histone H4 is the preferred substrate
-
-
-
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
histone H4 is the preferred substrate
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
histone H4 is the preferred substrate
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
P53930
acetylation of histone H4 by NuA4 is required for the cellular resistance to spindle stress. The NuA4 histone acetyltransferase subunit Yaf9, is required for the cellular response to spindle stress in yeast
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
exclusively acetylates of Lys16 of histone H4, the enzyme is required for bulk of H4 lysine 16 acetylation in vivo, role of SAS complex in antagonizing the speading of Sir proteins at silent loci in Saccharomyces cerevisiae
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
RmtA is specific for histone H4 with Arg3 as the methylation site. Methylation of histone H4 by recombinant RmtA affects the acetylation by p300/CBP, supporting aninterrelation of histone methylation and acetylation in transcriptional regulation. Important role of the enzyme for chromatin modulating activity
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
specific acetylation of Lys16, reversible acetylation of histones play an important role in regulation of chromatin structure and function. HMOF has a role in DNA damage responseduring cell cycle progression
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
the level of HAT-B-dependent acK12H4 may be very low under normal growth condition
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
acetyltion of Lys16, acetylates free histones and weakly acetylates nucleosomes
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
MYST-related histone acetyltransferase complex NuA4 or SAS(SAS-I)
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
MYST-related histone acetyltransferase complex Tip60, GNAT-related histone acetyltransferase complex PCAF
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
RmtA is specific for histone H4 with Arg3 as the methylation site
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
specifically acetylates Lys12, and to a lesser extent Lys5 of free, non-chromatin-bound histone H4
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
acetylation at Lys16 by MYST1 is essential for chromatin remodeling and is used for regulation of gene expression in eukaryotes. The nucleosome is a disc-shaped octamer consisting of two heterotetramers formed by histones H3/H4 and histones H2A and H2B
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
both Lys12 and Lys5 of soluble, non-chromatin-bound histone H4 are in vivo targets of acetylation for the yeast HAT-B enzyme. Lys12/Lys5-acetylated histone H4 is bound to the HAT-B complex in the soluble cell fraction. Exchange of Lys for Arg at position 12 of histone H4 do not interfere with histone H4 association with the complex, but prevented acetylation on Lys5 by the HAT-B enzyme, in vivo as well as in vitro
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
H4R3 methylation, catalyzed by PRMT1, facilitates beta-globin transcription by regulating histone acetyltransferase binding, and histone H3 and H4 acetylation, overview
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
Hat1 is a primary enzyme for di-acetylating cytosolic histone H4 at Lys5 and Lys12 in the cytosol
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
HBO1 is an H4-specific histone acetylase, and is a coactivator of the DNA replication licensing factor Cdt1. HBO1 acetylase activity is essential for DNA licensing of replication origins, where it controls H4 acetylation at the origins. H4 acetylation at origins is cell-cycle regulated, with maximal activity at the G1/S transition
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
HBO1 regulates global histone H4 acetylation
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
Mof is required for sex chromosome dosage compensation acting in the MSL complex, which also contains Msl1-3, Mle, and RNA, to acetylate H4K16 and to increase gene transcription from the single male X chromosome
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
Mof is solely responsible for H4K16 acetylation in mouse blastocysts. Tip60 plays essential roles in cell cycle progression in vitro
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q9H7Z6
MSL-associated MOF acetylates nucleosomal histone H4 almost exclusively on Lys16, while NSL-associated MOF exhibits a relaxed specificity and also acetylates nucleosomal histone H4 on Lys5 and Lys8
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q9H7Z6
MYST1 specificity to Lys16 of histone H4 is not absolute, because in experiments in vitro the protein is also able to acetylate histones H3 and H2A, whereas in vivo only modification of histone H4 is specific. Acetylation at Lys16 by MYST1 is essential for chromatin remodeling and is used for regulation of gene expression in eukaryotes. The nucleosome is a disc-shaped octamer consisting of two heterotetramers formed by histones H3/H4 and histones H2A and H2B. All human autosomes are susceptible to histone H4 acetylation by Lys16 residue and acetyltransferase MYST1
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
acetylation at Lys16
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
acetylation at Lys16 by MYST1
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q9H7Z6
acetylation at Lys16 by MYST1
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
activity with synthetic histone H4 tail peptide substrate of p300 that shows different degrees of autoacetylation, overview. Tyr1467 appears to serve as a general acid protonating the departing coenzyme A sulfur
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
full-length histone H4 is acetylated 2000fold faster than histone tail peptides
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
PCAF binds to dimethyl-Arg3 at histone H4 tails, dimethyl H4R3 provides a binding surface for PCAF and directly enhances histone H3 and H4 acetylation in vitro
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
recombinant ATAC2 has a weak HAT activity directed to histone H4
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q9H8E8
recombinant ATAC2 has a weak HAT activity directed to histone H4
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
recombinant Hbo1 can acetylate nucleosomal histone H4 in vitro, with a preference for Lys5 and Lys12, mapping of acetylation sites, overview
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
substrate is a H4 peptide
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
substrate is H4 peptide
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
substrates are synthetic N-terminal H4 peptides. The HAT-B complex acetylates only Lys12, recombinant Hat1 is able to modify Lys12 and Lys5. Exchange of Lys for Arg at position 12 of histone H4 does not interfere with histone H4 association with the complex, but prevents acetylation on Lys5 by the HAT-B enzyme, in vivo as well as in vitro
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Homo sapiens GCN5
-
histone H4 is the preferred substrate
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Homo sapiens GCN5
-
PCAF protein
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Tetrahymena thermophila GCN5
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Zea mays Cuzco
-
enzyme form B
mono- and diacetylated products
?
acetyl-CoA + histone H4
CoA + diacetylhistone H4
show the reaction diagram
-
-
mono-, di- and triacetylated products
?
acetyl-CoA + histone H4
CoA + diacetylhistone H4
show the reaction diagram
Zea mays, Zea mays Cuzco
-
enzyme form B
mono- and diacetylated products
?
acetyl-CoA + histone H4
CoA + triacetylhistone H4
show the reaction diagram
-
enzyme form B
mono-, di- and triacetylated products
?
acetyl-CoA + histone H4
Nalpha-acetylated-histone H4 + CoA
show the reaction diagram
Q04751
acetylation of N-terminal Ser
-
-
?
acetyl-CoA + histone H4
peptide CoA + acetylhistone H4 peptide
show the reaction diagram
P53930
-
-
-
?
acetyl-CoA + histone H4
peptide CoA + acetylhistone H4 peptide
show the reaction diagram
-
specific acetylation of Lys16
-
-
?
acetyl-CoA + histone H4 peptide
CoA + acetylpeptide of histone H4
show the reaction diagram
O14929
residues 1-20 of histone H4
-
-
?
acetyl-CoA + histone H4 peptide
CoA + actylhistone H4
show the reaction diagram
-
residues 2-24 of human histone H4
-
-
-
acetyl-CoA + NF-kB p65
CoA + acetyl-NF-kB p65
show the reaction diagram
-
acetylation of the subunit at Lys310 by p300 or PCAF
-
-
?
acetyl-CoA + non-histone chromatin high-mobility group protein
CoA + acetylated non-histone chromatin high-mobility group protein
show the reaction diagram
-
or chymotryptic peptides of
-
-
?
acetyl-CoA + p50 protein
CoA + acetyl-p50 protein
show the reaction diagram
-
acetylation of p50 by p300 independent of shear stress, acetylation of histones H4 at the site of SSRE within the eNOS promoter
-
-
?
acetyl-CoA + p53
CoA + acetyl-p53
show the reaction diagram
Q9H7Z6
p53 protein-acetylation of the Lys120 residue, p53 protein-acetylation of the Lys120 residue is carried out by acetyltransferases MYST1 and TIP60 to approximately equal extent
-
-
?
acetyl-CoA + p65 protein
CoA + acetyl-p65 protein
show the reaction diagram
-
acetylation of p65 by p300 during translocation into the nuclei in response to shear stress, acetylation of histones H3 at the site of SSRE within the eNOS promoter
-
-
?
acetyl-CoA + poly-L-lysine
CoA + N6-acetyllysine
show the reaction diagram
-
enzyme form A, not enzyme form B1 and B2
-
?
acetyl-CoA + protamine sulfate
?
show the reaction diagram
-
enzyme form A, not enzyme form B
-
-
?
acetyl-CoA + protamine sulfate
?
show the reaction diagram
-
enzyme form A, not enzyme form B
-
-
?
acetyl-CoA + protein p53
CoA + acetylprotein p53
show the reaction diagram
-
-
-
-
r
acetyl-CoA + protein p53
CoA + acetylprotein p53
show the reaction diagram
Q09472
-
-
-
?
acetyl-CoA + protein p53
CoA + acetylprotein p53
show the reaction diagram
-
substrate is a DNA-binding transcription activator and a tumor suppressor
-
-
?
acetyl-CoA + protein p53
CoA + acetylprotein p53
show the reaction diagram
-
peptide of p53 is a substrate for PCAF catalytic domain
-
-
?
acetyl-CoA + spermidine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + spermidine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + spermidine
?
show the reaction diagram
-
enzyme form B, not enzyme form A
-
-
?
acetyl-CoA + spermine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + spermine
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + spermine
?
show the reaction diagram
-
enzyme form A inactive, enzyme form B active
-
-
?
acetyl-CoA + transcription factor TFIIE
CoA + acetylated transcription factor TFIIE
show the reaction diagram
-
substrate is a basal transcription factor
-
-
?
acetyl-CoA + transcription factor TFIIF
CoA + acetylated transcription factor TFIIF
show the reaction diagram
-
substrate is a basal transcription factor
-
-
?
activated RNA polymerase II transcriptional coactivator p15 + 4-pentynoyl-CoA
?
show the reaction diagram
-
-
-
-
?
alpha-tubulin + acetyl-CoA
acetyl-alpha-tubulin + CoA
show the reaction diagram
-
-
-
-
?
androgen receptor + acetyl-CoA
acetylated androgen receptor + CoA
show the reaction diagram
-
receptor signaling in prostate cancer cells is augmented by the androgen receptor coactivator p300, which transactivates and acetylates the androgen receptor in the presence of 100 nM dihydrotestosterone, involvement of p300 in neuropeptide activation of androgen receptor signaling, overview, the KLKK motif of androgen receptor protein is both necessary and sufficient for acetylation by p300 in the presence of 100 nM dihydrotestosterone
-
-
?
ATM kinase + acetyl-CoA
acetylated ATM kinase + CoA
show the reaction diagram
-
ATM protein kinase regulates the cells response to DNA damage through the phosphorylation of proteins involved in cell-cycle checkpoints and DNA repair, suppression of Tip60 blocks the activation of ATMs kinase activity, ATM autophosphorylation e.g. at Ser1981, and prevents the ATM-dependent phosphorylation of p53 and chk2, inactivation of Tip60 sensitizes cells to ionizing radiation, overview, the ataxia telangiectasia mutant, i.e. ATM, protein kinase, ATM forms a stable complex with Tip60 through the conserved, highly required FATC domain of ATM, the interaction between ATM and Tip60 is not regulated in response to DNA damage, but is specifically induced by DNA damage, overview, mutations in the FATC domain that abolish the interaction between ATM and Tip60
-
-
?
H4 peptide + acetyl-CoA
?
show the reaction diagram
-
-
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
-
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
Q08649
-
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
histone acetylation is an important posttranslational modification correlated with gene activation, the HAC1 is involved in the regulation of flowering time via repression of flowering locus C, the enzyme participates in many physiological processes, including proliferation, differentiation, and apoptosis
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
key enzyme in post-translational modification of histones associated with transcriptionally active genes
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
phosphorylation of p300 at Ser1834 by the kinase Akt is essential for its histone acetyltransferase and transcriptional activity
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
histones from the Hela cell core
-
-
?
histone + propionyl-CoA
propionyl-histone + CoA
show the reaction diagram
Q08649
-
-
-
?
histone H2A + acetyl-CoA
acetyl-histone H2A + CoA
show the reaction diagram
-
acetylation of the tail of the histone, the enzyme is organized in the NuA4 subcomplex acting on the nucleosome, overview
-
-
?
histone H2B + acetyl-CoA
acetyl-histone H2B + CoA
show the reaction diagram
-
acetylation of the tail of the histone, the enzyme is organized in the catalytic Ada2/Ada3/Gcn5 subcomplex of SAGA acting on the nucleosome, overview
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
-
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
-
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
regulation
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
acetylation of Lys9, and Lys14
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
acetylation of Lys9, and Lys14
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
the enzyme is involved in ethanol-induced acetylation of histone H3 in hepatocytes, potential mechanism for gene expression activation by the enzyme, overview
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
acetylation at Lys9
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
acetylation at Lys9 and Lys14
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
acetylation of the tail of the histone, the enzyme is organized in the catalytic Ada2/Ada3/Gcn5 subcomplex of SAGA acting on the nucleosome, overview
-
-
?
histone H3 tail peptide + acetyl-CoA
acetyl-histone H3 tail peptide + CoA
show the reaction diagram
-
-
-
-
?
histone H3.2 + 4-pentynoyl-CoA
?
show the reaction diagram
-
-
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
-
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
-
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
Q08649
-
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
Q9UTM7
-
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
acetylation of Lys5, Lys8, and Lys12, Gcn5 and transcriptional adaptor Ada2a are involved in nucleosomal histone H4 acetylation
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
Q9UTM7
acetylation at Lys5 and Lys12, acetylation by Hat1 requires positively charged amino acids at positions 8 and 16 of the H4 tail
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
acetylation at Lys8, NCOAT has the ability to directly associate with both an acetylated and unacetylated histone H4 tail in vitro without tequiring acetyl-lysine contacts, binding and interaction mechanism, overview
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
acetylation of Lys5, Lys8, and Lys12
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
acetylation of Lys5, Lys8, and Lys12
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
acetylation of the tail of the histone, the enzyme is organized in the NuA4 subcomplex acting on the nucleosome, overview
-
-
?
histone H4 N-terminal peptide + acetyl-CoA
acetyl-histone H4 N-terminal peptide + CoA
show the reaction diagram
Q9UTM7
N-terminal peptide of histone H4 of different length and sequence, prepared in HeLa cell extract, overview, acetylation by Hat1 requires positively charged amino acids at positions 8 and 16 of the H4 tail, substituting glutamine for lysine at Lys8 and Lys16 dramatically reduces the ability of yHat1p to acetylate the H4 tail peptide, phosphorylation of Ser1 also reduces the acetylation of H4 peptides
-
-
?
isoform 1 of DNA polymerase zeta catalytic subunit + 4-pentynoyl-CoA
?
show the reaction diagram
-
-
-
-
?
isoform 1 of transcription factor BTF3 + 4-pentynoyl-CoA
?
show the reaction diagram
-
-
-
-
?
isoform 2 of protein SET + 4-pentynoyl-CoA
?
show the reaction diagram
-
-
-
-
?
nucleolin + 4-pentynoyl-CoA
?
show the reaction diagram
-
-
-
-
?
piccoloNuA4 peptide + acetyl-CoA
acetyl-piccoloNuA4 peptide + CoA
show the reaction diagram
Q08649
the peptide is part of the physiologic enzme complex, overview
-
-
?
piccoloNuA4 peptide + propionyl-CoA
propionyl-piccoloNuA4 peptide + CoA
show the reaction diagram
Q08649
-
-
-
?
promyelotic leukemia zinc finger gene + acetyl-CoA
acetylated promyelotic leukemia zinc finger gene + CoA
show the reaction diagram
-
i.e. PLZF, a direct and specific substrate of the p300 HAT, no activity with the substrate deletion mutant lacking zinc fingers 6 to 9
-
-
?
isoform long of antigen KI-67 + 4-pentynoyl-CoA
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
diaminodipropylamine and 1,3-propanediamine are no substrates
-
-
-
additional information
?
-
-
HeLa nucleosome or core histones are no substrate for recombinant SAS complex
-
-
-
additional information
?
-
-
protamine, bovine serum albumin, and ubiquitin are no substrates
-
-
-
additional information
?
-
-
for acetylation activity of Sas2, Sas4 is absolutely required, while Sas5 stimulate
-
-
-
additional information
?
-
-
Gcn5 is a coactivator of transcription
-
-
-
additional information
?
-
-
Gcn5 and PCAF protein are transcription cofactors
-
-
-
additional information
?
-
-
Gcn5 and PCAF protein are transcription cofactors
-
-
-
additional information
?
-
-
Gcn5 and PCAF protein are transcription cofactors
-
-
-
additional information
?
-
-
Gcn5 and PCAF protein are transcription cofactors
-
-
-
additional information
?
-
-
enzyme activity is regulated by phosphorylation and interaction with other regulating protein factors
-
-
-
additional information
?
-
-
MOZ and MORF genes are rearranged by chromosome abnormalities associated with several types of leukemia
-
-
-
additional information
?
-
-
MYST-related histone acetyltransferase complex NuA4: required for cell growth, required for p53-dependent transcription activation in yeast, presumably through its Yng2 subunit, homolog of the tumor suppressor ING1. GNAT-related histone acetyltransferase complex SAGA can stimulate Gal4-VP16 activation in a manner dependent on HAT activity. DSAGA can be recruited by several yeast activators. SAGA is targeted to promoter regions proximal to the activator binding site. Once targeted, SAGA acetylates histone h3 in the vicinity of the promoter. Targeted acetylation by SAGA stabilizes its binding and that of a targeted SWI/SNF chromatin-remodeling complex. SAGA is required for both activation of the yeast ARG1 promoter by Gcn4 activator and repression by the ArgR/Mcm1 repressor complex
-
-
-
additional information
?
-
-
MYST-related histone acetyltransferase complex Tip60 also acts as a transcriptional coactivator in several systems including class I nuclear hormone receptors, NF-kappaB and at the superoxide dismutase gene. Tip 60 has been implicated in Alzheimer disease because it stimulates transcription when asociated with the cleaved cytoplasmic tail fragment of the amyloid-beta precursor protein
-
-
-
additional information
?
-
-
Tip60 plays a role in the control of cell-related events
-
-
-
additional information
?
-
Q04751
no acetylation of adrenocorticotropin or a H3 peptide
-
-
-
additional information
?
-
-
acetylation of proteins by the enzyme plays a critical role in the regulation of gene expression
-
-
-
additional information
?
-
-
androgen Src kinase and PKCd kinase are involved in the regulation of p300 HAT activity via bombesin, overview
-
-
-
additional information
?
-
-
deregulated HAT activity plays a role in the development of a range of cancers
-
-
-
additional information
?
-
-
the enzyme is involved in Sp1 activation of the cyclin D1 promoter, TAF1-dependent histone acetylation facilitates transcription factor binding to the Sp1 sites, thereby activating cyclin D1 transcription and ultimately G1-to-S-phase progression, regulation, overview
-
-
-
additional information
?
-
-
the enzyme modulates gene expression in liver nuclei in an epigenetic manner at high blood alcohol levels, no alteratins of MAP kinase levels, overview
-
-
-
additional information
?
-
-
the enzyme plays a role in chromatin structure and gene expression regulation as a catalytic component of multiprotein complexes, some of which also contain Ada2-type transcriptional coactivators
-
-
-
additional information
?
-
-
the histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic leukemia zinc finger protein
-
-
-
additional information
?
-
-
in absence of acceptor substrate the enzyme performs autoacetylation, identification of 13 autoacetylation sites and mechanism, autoacetylation catalyzed by p300 HAT is about 1000-fold more efficient than p300/CREB-binding protein-associated factor-mediated acetylation of catalytically defective p300 HAT, overview
-
-
-
additional information
?
-
Q08649
in the absence of histone acceptor, slow rates of enzyme autoacetylation and of CoA formation occur
-
-
-
additional information
?
-
Q9UTM7
substrate specificity and order of acetylation of histone H4 by Hat1, overview
-
-
-
additional information
?
-
-
the nuclear cytoplasmic O-GlcNAcase and acetyltransferase, NCOAT, is a bifunctional enzyme with both glycoside hydrolase and alkyltransferase activity and contains a zinc finger-like motif responsible for substrate recognition, via making contacts with the histone tails within nucleosomes, essential for activity, overview
-
-
-
additional information
?
-
-
MOZ specifically interacts and associates with transcription factors such as AML1, PU.1, p53, Runx2 and NF-kappaB, functioning as their transcriptional coactivator and cooperatively activating target gene transcription
-
-
-
additional information
?
-
-
a homologue of Moz, zMoz, occurs in zebrafish to perform a potential Moz function in the trunk region
-
-
-
additional information
?
-
-
affects the inflorescence meristem and stamen development in Arabidopsis thaliana
-
-
-
additional information
?
-
-
ATAC2 associates with GCN5 and other proteins linked to chromatin metabolism
-
-
-
additional information
?
-
Q9H8E8
ATAC2 associates with GCN5 and other proteins linked to chromatin metabolism
-
-
-
additional information
?
-
Q9H7Z6
besides the male-specific lethal, MSL, HAT complex, MOF is also a component of the second HAT complex, designated the non-specific lethal, NSL complex, substrate specificity of the NSL complex, overview. Assembly of the MOF HAT into MSL or NSL complexes controls its substrate specificity
-
-
-
additional information
?
-
-
Esa1 is the catalytic subunit of at least two multiprotein complexes, NuA4 and Piccolo NuA4, picNuA4
-
-
-
additional information
?
-
-
HATs perform a conserved mechanism of acetyl-transfer, where the lysine-containing substrate directly attacks enzyme-bound acetyl-CoA. The ability of HATs to form distinct multi-subunit complexes provide a means to regulate HAT activity by altering substrate specificity, targeting to specific loci, enhancing acetyltransferase activity, restricting access of non-target proteins, and coordinating the multiple enzyme activities of the complex
-
-
-
additional information
?
-
-
HBO1 histone acetylase is involved in DNA replication licensing and associates with replication origins, located within the HPRT1 coding sequence, specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor geminin
-
-
-
additional information
?
-
-
HBO1 occurs as a component of a multiprotein complex with histone H3 and H4 acetyltransferase activity in 293 cells. The mammalian complex corresponding to the yeast NuA4 complex contains the MYST HAT Tip60
-
-
-
additional information
?
-
-
HBO1, Sas2 and Sas3 are involved in transcriptional repression enhancing Sir1-mediated epigenetic gene silencing. NuA3 and NuA4 complexes contain the MYST HATs Sas3 and Esa1, respectively. Sas2 histone acetylation of H4K16 opposed by Sir2 deacetylation of H4K16 at the euchromatin/heterochromatin interface maintains the boundary between regions of transcriptionally active and silent telomeric chromatin. Esa1 plays a role in maintaining the integrity of the DNA, rather than open chromatin structure and high-level transcriptional activity
-
-
-
additional information
?
-
-
histone acetylation and deacetylation is an epigenetic mechanism in volved in regulation of mIRNA production. GCN5 has a general repressive effect on microRNAs, miRNAs, and it targets a subset of MIRNA genes, GCN5 is required for acetylation of histone H3 lysine 14 at these loci, overview
-
-
-
additional information
?
-
-
histones H3 and H4 and their chaperone Asf1, including RbAp48, a regulatory subunit of Hat1 enzyme, are associated with Hat1 in the cytosol of chicken cells. Hat1 regulates integrity of cytosolic histone H3-H4 containing complex, effect of Hat1 on status for the cytosolic histones H3/H4 pre-deposition complexes with respective chaperone proteins, overview
-
-
-
additional information
?
-
-
in addition to Asf1, Rtt109 is also functionally linked to Rtt101, Mms1, and Mms22
-
-
-
additional information
?
-
-
in response to DNA damage, Tip60 acetylates ATM, a DNA damage related kinase, allowing for phosphorylation of Chk2 and p53 by ATM. HATs perform a conserved mechanism of acetyl-transfer, where the lysine-containing substrate directly attacks enzyme-bound acetyl-CoA. The ability of HATs to form distinct multi-subunit complexes provide a means to regulate HAT activity by altering substrate specificity, targeting to specific loci, enhancing acetyltransferase activity, restricting access of non-target proteins, and coordinating the multiple enzyme activities of the complex
-
-
-
additional information
?
-
-
increase in eNOS mRNA, caused by shear stress, is completely blocked by pharmacological inhibition of p300/HAT activity with curcumin or by p300 small interfering RNA
-
-
-
additional information
?
-
-
mechanistically, p300 acts as a transcriptional coactivator through the direct interaction with a diverse set of transcription factors and RNA polymerase II transcription machinery
-
-
-
additional information
?
-
-
Mst1 interacts with a number of proteins involved in chromosome integrity and centromere function, including the methyltransferase Skb1, the recombination mediator Rad22, Sc Rad52, the chromatin assembly factor Hip1, Sc Hir1, and the Msc1 protein related to a family of histone demethylases, detailed interaction analysis, overview
-
-
-
additional information
?
-
-
MYST1 is a part of multiprotein complexes that accomplish functions of male X chromosome activation and thereby functions of dosage compensation in Drosophila and, in mammals, global acetylation of histone H4 K16. Functional links between MYST1 and proteins ATM and p53. Interactions between MSL1 and MYST1 within the MSL complex in Drosophila melanogaster, the compensasome includes proteins MSL1, MSL2, MSL3, MLE, MOF, a histone acetyltransferase homologous to MYST1, JIL1, and two non-coding RNA: roX1 and roX2, structure and function of the compensasome, detailed overview. Cell interactome fragments including protein homologs of hampin and MYST1, overview
-
-
-
additional information
?
-
Q9H7Z6
MYST1 is a part of multiprotein complexes that accomplish functions of male X chromosome activation and thereby functions of dosage compensation in Drosophila and, in mammals, global acetylation of histone H4 K16. Functional links between MYST1 and proteins ATM and p53. MYST1 interacts with WDR5. Cell interactome fragments including protein homologs of hampin and MYST1, overview
-
-
-
additional information
?
-
-
PCAF is present in USF1/PRMT1 complexes
-
-
-
additional information
?
-
-
Rtt109 facilitates error-free replication to prevent CAG/CTG repeat contractions. The Rtt107/Rtt101 complex is recruited to stalled replication forks in an Rtt109-dependent manner
-
-
-
additional information
?
-
-
soluble histone H4 Hat1-dependently acetylated on Lys12 is present in cells arrested at all cell cycle stages, G1, S, G2/M and also G0. Histone H3 seems to be no substrate for the HAT-B complex
-
-
-
additional information
?
-
-
specific role of MOZ-driven acetylation in controlling a desirable balance between proliferation and differentiation during hematopoiesis. MOZ also shows activity either as Runx1 coactivator or in the induction of leukemic transformation via transcriptional intermediary factor 2, TIF2, but is not essentially required
-
-
-
additional information
?
-
-
the mammalian complex corresponding to the yeast NuA4 complex contains the MYST HAT Tip60. Myc recruits the Tip60 complex to the chromatin in Rat1 wild-type cells, but not in Rat1 Myc mutant cells. Hbo1 appears to function predominantly in transcriptional repression
-
-
-
additional information
?
-
-
Tip60 is part of the evolutionarily conserved NuA4 complex
-
-
-
additional information
?
-
-
Tip60, in complex with homologues of the mammalian Tip60 complex, exhibits functional redundancy with two other groups of genes, known as synthetic multivulva A and B genes, synMUV. Therefore, the genes encoding proteins of the Tip60 complex are termed class C synMUV genes. SynMUV A and B counteract EGF to Ras to MAPK signaling and the Tip60 complex is a chromatin-modifying complex
-
-
-
additional information
?
-
-
Gcn5 and p300 appear to be constituitive HATs that do not require helper proteins to exhibit full catalytic activity. Esa1 and Rtt109 represent low-activity HATs that are stimulated by regulatory helper proteins, Yng2-Epl1 and Vps75/Asf1, respectively. p300/CBP exhibits the broadest protein specificity, p300 prefers histone acetylation sites with a positive charge in the -3 or +4 position. Ability of some HATs to utilize longer chain acyl-CoA, i.e. propionyl-CoA
-
-
-
additional information
?
-
-
Gcn5 appears to be constituitive HAT that does not require helper proteins to exhibit full catalytic activity
-
-
-
additional information
?
-
-
HBO1 interacts both with transcriptional activator proteins and with MCM2 and ORC1
-
-
-
additional information
?
-
Q9H7Z6
MYST1 specificity to Lys16 of histone H4 is not absolute, because in experiments in vitro the protein is also able to acetylate histones H3 and H2A, whereas in vivo only modification of histone H4 is specific
-
-
-
additional information
?
-
Q9H7Z6
no activity with histones H3, H2A and H2B by recombinant MOF in HeLa nucleosomes
-
-
-
additional information
?
-
-
p/CAF and p300 appear to be constitutive HATs that do not require helper proteins to exhibit full catalytic activity
-
-
-
additional information
?
-
-
p300 performs autoacetylation, overview
-
-
-
additional information
?
-
Q9AR19
isoform GCN5 is not the enzyme responsible for histone acetylation at cold-regulated genes COR promoters during cold acclimation
-
-
-
additional information
?
-
Q9H7Z6
assembly of the enzyme into male-specific lethal, MSL, or non-specific lethal, NSL, complexes controls its substrate specificity. Although MSL-associated enzyme acetylates nucleosomal histone H4 almost exclusively on lysine 16, NSL-associated enzyme exhibits a relaxed specificity and also acetylates nucleosomal histone H4 on lysines 5 and 8
-
-
-
additional information
?
-
O14929
Glu187 and Glu276 could act as the general catalytic base and together with Asp277 have a cumulative effect on deprotonation of the ?-amino group of substrate Lys12 of the histone H4 peptide, which could be mediated by water molecules
-
-
-
additional information
?
-
-
histone chaperone Vps75 acts as activiating subunit. The rate-determining step of the activated complex is the transfer of the acetyl group from acetyl-CoA to the acceptor lysine residue. Vps75 stimulates catalysis more than 250fold, not by contributing a catalytic base, but by stabilizing the catalytically active conformation of enzyme Rtt109
-
-
-
additional information
?
-
Q8III2
protein participates in var gene activation
-
-
-
additional information
?
-
-
removal of lysine residues does not substantially affect the ability of NuA4 histone actyltransferase complex to acetylate remaining sites, and insertion of an additional lysine into the substrate histone H4 tail leads to rapid quintuple-acetylation. Conversion of the native histone H2A tail to an H4-like sequence results in enhanced multi-site acetylation. NuA4's site selectivity is dictated by accessibility on the nucleosome surface, the relative proximity from the histone fold domain, and a preference for intervening glycine residues with a minimal (n + 2) spacing between lysines
-
-
-
additional information
?
-
Saccharomyces cerevisiae BY4705
-
Rtt109 facilitates error-free replication to prevent CAG/CTG repeat contractions. The Rtt107/Rtt101 complex is recruited to stalled replication forks in an Rtt109-dependent manner
-
-
-
additional information
?
-
Homo sapiens GCN5
-
Gcn5 and PCAF protein are transcription cofactors, enzyme activity is regulated by phosphorylation and interaction with other regulating protein factors
-
-
-
additional information
?
-
Homo sapiens GCN5
-
Gcn5 is a coactivator of transcription, Gcn5 and PCAF protein are transcription cofactors
-
-
-
additional information
?
-
Mus musculus C57BL/6
-
specific role of MOZ-driven acetylation in controlling a desirable balance between proliferation and differentiation during hematopoiesis. MOZ also shows activity either as Runx1 coactivator or in the induction of leukemic transformation via transcriptional intermediary factor 2, TIF2, but is not essentially required
-
-
-
additional information
?
-
Danio rerio zMoz
-
a homologue of Moz, zMoz, occurs in zebrafish to perform a potential Moz function in the trunk region
-
-
-
additional information
?
-
Tetrahymena sp. GCN5
-
Gcn5 appears to be constituitive HAT that does not require helper proteins to exhibit full catalytic activity
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4 acetyl-CoA + histone H4
4 CoA + tetraacetylhistone H4
show the reaction diagram
-
-
NuA4 randomly acetylates free and nucleosomal H4, with a small preference for lysines 5, 8, and 12 over 16
-
?
acetyl-CoA + beta-site amyloid precursor protein-cleaving enzyme 1
CoA + acetylated beta-site amyloid precursor protein-cleaving enzyme 1
show the reaction diagram
-
-
-
-
?
acetyl-CoA + c-Myc
CoA + acetylated c-Myc
show the reaction diagram
-
acetylation by Tip60 increases c-Myc protein stability in transfected H-1299 human lung carcinoma cells
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
-
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone H1 is not acetylated in vivo
-
-
-
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
Esa1 protein is involved in cell cycle regulation
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
involved in chromatin remodeling and DNA repair
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone H3 is the preferred substrate
-
r
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
neutralization of positively charged lysine residues by acetylation lowering the affinity of histone octamers for the negatively charged DNA
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
involved in dynamic equilibrium of core histone acetylation
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
most likely involved in acetylation of newly synthesized histones in cytoplasm prior to chromatin assembly
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
important role of the enzyme for chromatin modulating activity
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Q9EQQ9
the bifunctional enzyme NCOAT, nuclear cytoplasmic O-GlcNacase and acetyltransferase, may be regulated to reduce the state of glycosylation of transcriptional activators while increasing the acetylation of histones to allow for concerted activation of eukaryotic gene transcription
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
-
histone acetylation on Lys16 by Sas2
-
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
histone H1 is not acetylated in vivo
-
-
-
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
neutralization of positively charged lysine residues by acetylation lowering the affinity of histone octamers for the negatively charged DNA, the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling
-
?
acetyl-CoA + histone
CoA + acetylhistone
show the reaction diagram
Homo sapiens GCN5
-
involved in chromatin remodeling and DNA repair, histone H3 is the preferred substrate
-
r
acetyl-CoA + histone H
CoA + acetylhistone H
show the reaction diagram
-
histone acetyltransferase AtGCN5 is required to regulate the floral meristem activity through the WUS/AG pathway
-
-
?
acetyl-CoA + histone H2A
CoA + acetylhistone H2A
show the reaction diagram
-
acetylation at Lys5 by Tip60
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys14 by tGCN5 in the consensus sequence QTARKSTGGK14APRKLASK
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
acetylation of Lys56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
CBP binds and acetylates histones at neural promoters, and regulates Corpus Callosum development. CBP binds to neuronal and glial promoters and globally promotes histone acetylation in the embryonic cortex, e.g. the beta-actin promoter, overview
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
H4R3 methylation, catalyzed by PRMT1, facilitates beta-globin transcription by regulating histone acetyltransferase binding, and histone H3 and H4 acetylation, overview
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
regulation, detailed overview. Acetylation and deacetylation events, in combination with other post-translational protein modifications, generate an NF-kappaB-signaling code and regulate NF-kappaB-dependent gene transcription in an inducer- and promoter-dependent manner, overview
-
-
r
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Rtt109 association with distinct histone chaperones directs substrate selection between N-terminal lysines, H3K9, H3K23, and those within the histone fold domain, H3K56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
-
Rtt109 is specific for histone H3, acetylation at Lys9 and Lys56. RTT109 has functions in addition to maintaining genome stability
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Saccharomyces cerevisiae BY4705
-
acetylation of Lys56
-
-
?
acetyl-CoA + histone H3
CoA + acetylhistone H3
show the reaction diagram
Tetrahymena sp. GCN5
-
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
-
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
P53930
acetylation of histone H4 by NuA4 is required for the cellular resistance to spindle stress. The NuA4 histone acetyltransferase subunit Yaf9, is required for the cellular response to spindle stress in yeast
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
exclusively acetylates of Lys16 of histone H4, the enzyme is required for bulk of H4 lysine 16 acetylation in vivo, role of SAS complex in antagonizing the speading of Sir proteins at silent loci in Saccharomyces cerevisiae
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
RmtA is specific for histone H4 with Arg3 as the methylation site. Methylation of histone H4 by recombinant RmtA affects the acetylation by p300/CBP, supporting aninterrelation of histone methylation and acetylation in transcriptional regulation. Important role of the enzyme for chromatin modulating activity
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
specific acetylation of Lys16, reversible acetylation of histones play an important role in regulation of chromatin structure and function. HMOF has a role in DNA damage responseduring cell cycle progression
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
the level of HAT-B-dependent acK12H4 may be very low under normal growth condition
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
acetylation at Lys16 by MYST1 is essential for chromatin remodeling and is used for regulation of gene expression in eukaryotes. The nucleosome is a disc-shaped octamer consisting of two heterotetramers formed by histones H3/H4 and histones H2A and H2B
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
both Lys12 and Lys5 of soluble, non-chromatin-bound histone H4 are in vivo targets of acetylation for the yeast HAT-B enzyme. Lys12/Lys5-acetylated histone H4 is bound to the HAT-B complex in the soluble cell fraction. Exchange of Lys for Arg at position 12 of histone H4 do not interfere with histone H4 association with the complex, but prevented acetylation on Lys5 by the HAT-B enzyme, in vivo as well as in vitro
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
H4R3 methylation, catalyzed by PRMT1, facilitates beta-globin transcription by regulating histone acetyltransferase binding, and histone H3 and H4 acetylation, overview
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
Hat1 is a primary enzyme for di-acetylating cytosolic histone H4 at Lys5 and Lys12 in the cytosol
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
HBO1 is an H4-specific histone acetylase, and is a coactivator of the DNA replication licensing factor Cdt1. HBO1 acetylase activity is essential for DNA licensing of replication origins, where it controls H4 acetylation at the origins. H4 acetylation at origins is cell-cycle regulated, with maximal activity at the G1/S transition
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
HBO1 regulates global histone H4 acetylation
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
Mof is required for sex chromosome dosage compensation acting in the MSL complex, which also contains Msl1-3, Mle, and RNA, to acetylate H4K16 and to increase gene transcription from the single male X chromosome
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
-
Mof is solely responsible for H4K16 acetylation in mouse blastocysts. Tip60 plays essential roles in cell cycle progression in vitro
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q9H7Z6
MSL-associated MOF acetylates nucleosomal histone H4 almost exclusively on Lys16, while NSL-associated MOF exhibits a relaxed specificity and also acetylates nucleosomal histone H4 on Lys5 and Lys8
-
-
?
acetyl-CoA + histone H4
CoA + acetylhistone H4
show the reaction diagram
Q9H7Z6
MYST1 specificity to Lys16 of histone H4 is not absolute, because in experiments in vitro the protein is also able to acetylate histones H3 and H2A, whereas in vivo only modification of histone H4 is specific. Acetylation at Lys16 by MYST1 is essential for chromatin remodeling and is used for regulation of gene expression in eukaryotes. The nucleosome is a disc-shaped octamer consisting of two heterotetramers formed by histones H3/H4 and histones H2A and H2B. All human autosomes are susceptible to histone H4 acetylation by Lys16 residue and acetyltransferase MYST1
-
-
?
acetyl-CoA + p50 protein
CoA + acetyl-p50 protein
show the reaction diagram
-
acetylation of p50 by p300 independent of shear stress
-
-
?
acetyl-CoA + p53
CoA + acetyl-p53
show the reaction diagram
Q9H7Z6
p53 protein-acetylation of the Lys120 residue
-
-
?
acetyl-CoA + p65 protein
CoA + acetyl-p65 protein
show the reaction diagram
-
acetylation of p65 by p300 during translocation into the nuclei in response to shear stress
-
-
?
androgen receptor + acetyl-CoA
acetylated androgen receptor + CoA
show the reaction diagram
-
receptor signaling in prostate cancer cells is augmented by the androgen receptor coactivator p300, which transactivates and acetylates the androgen receptor in the presence of 100 nM dihydrotestosterone, involvement of p300 in neuropeptide activation of androgen receptor signaling, overview
-
-
?
ATM kinase + acetyl-CoA
acetylated ATM kinase + CoA
show the reaction diagram
-
ATM protein kinase regulates the cells response to DNA damage through the phosphorylation of proteins involved in cell-cycle checkpoints and DNA repair, suppression of Tip60 blocks the activation of ATMs kinase activity, ATM autophosphorylation e.g. at Ser1981, and prevents the ATM-dependent phosphorylation of p53 and chk2, inactivation of Tip60 sensitizes cells to ionizing radiation, overview
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
-
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
Q08649
-
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
histone acetylation is an important posttranslational modification correlated with gene activation, the HAC1 is involved in the regulation of flowering time via repression of flowering locus C, the enzyme participates in many physiological processes, including proliferation, differentiation, and apoptosis
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
key enzyme in post-translational modification of histones associated with transcriptionally active genes
-
-
?
histone + acetyl-CoA
acetyl-histone + CoA
show the reaction diagram
-
phosphorylation of p300 at Ser1834 by the kinase Akt is essential for its histone acetyltransferase and transcriptional activity
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
-
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
-
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
regulation
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
acetylation of Lys9, and Lys14
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
acetylation of Lys9, and Lys14
-
-
?
histone H3 + acetyl-CoA
acetyl-histone H3 + CoA
show the reaction diagram
-
the enzyme is involved in ethanol-induced acetylation of histone H3 in hepatocytes, potential mechanism for gene expression activation by the enzyme, overview
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
-
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
-
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
Q9UTM7
-
-
-
?
piccoloNuA4 peptide + acetyl-CoA
acetyl-piccoloNuA4 peptide + CoA
show the reaction diagram
Q08649
the peptide is part of the physiologic enzme complex, overview
-
-
?
promyelotic leukemia zinc finger gene + acetyl-CoA
acetylated promyelotic leukemia zinc finger gene + CoA
show the reaction diagram
-
-
-
-
?
histone H4 + acetyl-CoA
acetyl-histone H4 + CoA
show the reaction diagram
-
acetylation of Lys5, Lys8, and Lys12, Gcn5 and transcriptional adaptor Ada2a are involved in nucleosomal histone H4 acetylation
-
-
?
additional information
?
-
-
enzyme activity is regulated by phosphorylation and interaction with other regulating protein factors
-
-
-
additional information
?
-
-
MOZ and MORF genes are rearranged by chromosome abnormalities associated with several types of leukemia
-
-
-
additional information
?
-
-
MYST-related histone acetyltransferase complex NuA4: required for cell growth, required for p53-dependent transcription activation in yeast, presumably through its Yng2 subunit, homolog of the tumor suppressor ING1. GNAT-related histone acetyltransferase complex SAGA can stimulate Gal4-VP16 activation in a manner dependent on HAT activity. DSAGA can be recruited by several yeast activators. SAGA is targeted to promoter regions proximal to the activator binding site. Once targeted, SAGA acetylates histone h3 in the vicinity of the promoter. Targeted acetylation by SAGA stabilizes its binding and that of a targeted SWI/SNF chromatin-remodeling complex. SAGA is required for both activation of the yeast ARG1 promoter by Gcn4 activator and repression by the ArgR/Mcm1 repressor complex
-
-
-
additional information
?
-
-
MYST-related histone acetyltransferase complex Tip60 also acts as a transcriptional coactivator in several systems including class I nuclear hormone receptors, NF-kappaB and at the superoxide dismutase gene. Tip 60 has been implicated in Alzheimer disease because it stimulates transcription when asociated with the cleaved cytoplasmic tail fragment of the amyloid-beta precursor protein
-
-
-
additional information
?
-
-
Tip60 plays a role in the control of cell-related events
-
-
-
additional information
?
-
-
acetylation of proteins by the enzyme plays a critical role in the regulation of gene expression
-
-
-
additional information
?
-
-
androgen Src kinase and PKCd kinase are involved in the regulation of p300 HAT activity via bombesin, overview
-
-
-
additional information
?
-
-
deregulated HAT activity plays a role in the development of a range of cancers
-
-
-
additional information
?
-
-
the enzyme is involved in Sp1 activation of the cyclin D1 promoter, TAF1-dependent histone acetylation facilitates transcription factor binding to the Sp1 sites, thereby activating cyclin D1 transcription and ultimately G1-to-S-phase progression, regulation, overview
-
-
-
additional information
?
-
-
the enzyme modulates gene expression in liver nuclei in an epigenetic manner at high blood alcohol levels, no alteratins of MAP kinase levels, overview
-
-
-
additional information
?
-
-
the enzyme plays a role in chromatin structure and gene expression regulation as a catalytic component of multiprotein complexes, some of which also contain Ada2-type transcriptional coactivators
-
-
-
additional information
?
-
-
the histone acetyltransferase activity of p300 is required for transcriptional repression by the promyelocytic leukemia zinc finger protein
-
-
-
additional information
?
-
-
MOZ specifically interacts and associates with transcription factors such as AML1, PU.1, p53, Runx2 and NF-kappaB, functioning as their transcriptional coactivator and cooperatively activating target gene transcription
-
-
-
additional information
?
-
-
a homologue of Moz, zMoz, occurs in zebrafish to perform a potential Moz function in the trunk region
-
-
-
additional information
?
-
-
affects the inflorescence meristem and stamen development in Arabidopsis thaliana
-
-
-
additional information
?
-
-
ATAC2 associates with GCN5 and other proteins linked to chromatin metabolism
-
-
-
additional information
?
-
Q9H8E8
ATAC2 associates with GCN5 and other proteins linked to chromatin metabolism
-
-
-
additional information
?
-
Q9H7Z6
besides the male-specific lethal, MSL, HAT complex, MOF is also a component of the second HAT complex, designated the non-specific lethal, NSL complex, substrate specificity of the NSL complex, overview. Assembly of the MOF HAT into MSL or NSL complexes controls its substrate specificity
-
-
-
additional information
?
-
-
Esa1 is the catalytic subunit of at least two multiprotein complexes, NuA4 and Piccolo NuA4, picNuA4
-
-
-
additional information
?
-
-
HATs perform a conserved mechanism of acetyl-transfer, where the lysine-containing substrate directly attacks enzyme-bound acetyl-CoA. The ability of HATs to form distinct multi-subunit complexes provide a means to regulate HAT activity by altering substrate specificity, targeting to specific loci, enhancing acetyltransferase activity, restricting access of non-target proteins, and coordinating the multiple enzyme activities of the complex
-
-
-
additional information
?
-
-
HBO1 histone acetylase is involved in DNA replication licensing and associates with replication origins, located within the HPRT1 coding sequence, specifically during the G1 phase of the cell cycle in a manner that depends on the replication licensing factor Cdt1, but is independent of the Cdt1 repressor geminin
-
-
-
additional information
?
-
-
HBO1 occurs as a component of a multiprotein complex with histone H3 and H4 acetyltransferase activity in 293 cells. The mammalian complex corresponding to the yeast NuA4 complex contains the MYST HAT Tip60
-
-
-
additional information
?
-
-
HBO1, Sas2 and Sas3 are involved in transcriptional repression enhancing Sir1-mediated epigenetic gene silencing. NuA3 and NuA4 complexes contain the MYST HATs Sas3 and Esa1, respectively. Sas2 histone acetylation of H4K16 opposed by Sir2 deacetylation of H4K16 at the euchromatin/heterochromatin interface maintains the boundary between regions of transcriptionally active and silent telomeric chromatin. Esa1 plays a role in maintaining the integrity of the DNA, rather than open chromatin structure and high-level transcriptional activity
-
-
-
additional information
?
-
-
histone acetylation and deacetylation is an epigenetic mechanism in volved in regulation of mIRNA production. GCN5 has a general repressive effect on microRNAs, miRNAs, and it targets a subset of MIRNA genes, GCN5 is required for acetylation of histone H3 lysine 14 at these loci, overview
-
-
-
additional information
?
-
-
histones H3 and H4 and their chaperone Asf1, including RbAp48, a regulatory subunit of Hat1 enzyme, are associated with Hat1 in the cytosol of chicken cells. Hat1 regulates integrity of cytosolic histone H3-H4 containing complex, effect of Hat1 on status for the cytosolic histones H3/H4 pre-deposition complexes with respective chaperone proteins, overview
-
-
-
additional information
?
-
-
in addition to Asf1, Rtt109 is also functionally linked to Rtt101, Mms1, and Mms22
-
-
-
additional information
?
-
-
in response to DNA damage, Tip60 acetylates ATM, a DNA damage related kinase, allowing for phosphorylation of Chk2 and p53 by ATM. HATs perform a conserved mechanism of acetyl-transfer, where the lysine-containing substrate directly attacks enzyme-bound acetyl-CoA. The ability of HATs to form distinct multi-subunit complexes provide a means to regulate HAT activity by altering substrate specificity, targeting to specific loci, enhancing acetyltransferase activity, restricting access of non-target proteins, and coordinating the multiple enzyme activities of the complex
-
-
-
additional information
?
-
-
increase in eNOS mRNA, caused by shear stress, is completely blocked by pharmacological inhibition of p300/HAT activity with curcumin or by p300 small interfering RNA
-
-
-
additional information
?
-
-
mechanistically, p300 acts as a transcriptional coactivator through the direct interaction with a diverse set of transcription factors and RNA polymerase II transcription machinery
-
-
-
additional information
?
-
-
Mst1 interacts with a number of proteins involved in chromosome integrity and centromere function, including the methyltransferase Skb1, the recombination mediator Rad22, Sc Rad52, the chromatin assembly factor Hip1, Sc Hir1, and the Msc1 protein related to a family of histone demethylases, detailed interaction analysis, overview
-
-
-
additional information
?
-
-
MYST1 is a part of multiprotein complexes that accomplish functions of male X chromosome activation and thereby functions of dosage compensation in Drosophila and, in mammals, global acetylation of histone H4 K16. Functional links between MYST1 and proteins ATM and p53. Interactions between MSL1 and MYST1 within the MSL complex in Drosophila melanogaster, the compensasome includes proteins MSL1, MSL2, MSL3, MLE, MOF, a histone acetyltransferase homologous to MYST1, JIL1, and two non-coding RNA: roX1 and roX2, structure and function of the compensasome, detailed overview. Cell interactome fragments including protein homologs of hampin and MYST1, overview
-
-
-
additional information
?
-
Q9H7Z6
MYST1 is a part of multiprotein complexes that accomplish functions of male X chromosome activation and thereby functions of dosage compensation in Drosophila and, in mammals, global acetylation of histone H4 K16. Functional links between MYST1 and proteins ATM and p53. MYST1 interacts with WDR5. Cell interactome fragments including protein homologs of hampin and MYST1, overview
-
-
-
additional information
?
-
-
PCAF is present in USF1/PRMT1 complexes
-
-
-
additional information
?
-
-
Rtt109 facilitates error-free replication to prevent CAG/CTG repeat contractions. The Rtt107/Rtt101 complex is recruited to stalled replication forks in an Rtt109-dependent manner
-
-
-
additional information
?
-
-
soluble histone H4 Hat1-dependently acetylated on Lys12 is present in cells arrested at all cell cycle stages, G1, S, G2/M and also G0. Histone H3 seems to be no substrate for the HAT-B complex
-
-
-
additional information
?
-
-
specific role of MOZ-driven acetylation in controlling a desirable balance between proliferation and differentiation during hematopoiesis. MOZ also shows activity either as Runx1 coactivator or in the induction of leukemic transformation via transcriptional intermediary factor 2, TIF2, but is not essentially required
-
-
-
additional information
?
-
-
the mammalian complex corresponding to the yeast NuA4 complex contains the MYST HAT Tip60. Myc recruits the Tip60 complex to the chromatin in Rat1 wild-type cells, but not in Rat1 Myc mutant cells. Hbo1 appears to function predominantly in transcriptional repression
-
-
-
additional information
?
-
-
Tip60 is part of the evolutionarily conserved NuA4 complex
-
-
-
additional information
?
-
-
Tip60, in complex with homologues of the mammalian Tip60 complex, exhibits functional redundancy with two other groups of genes, known as synthetic multivulva A and B genes, synMUV. Therefore, the genes encoding proteins of the Tip60 complex are termed class C synMUV genes. SynMUV A and B counteract EGF to Ras to MAPK signaling and the Tip60 complex is a chromatin-modifying complex
-
-
-
additional information
?
-
Q9AR19
isoform GCN5 is not the enzyme responsible for histone acetylation at cold-regulated genes COR promoters during cold acclimation
-
-
-
additional information
?
-
Saccharomyces cerevisiae BY4705
-
Rtt109 facilitates error-free replication to prevent CAG/CTG repeat contractions. The Rtt107/Rtt101 complex is recruited to stalled replication forks in an Rtt109-dependent manner
-
-
-
additional information
?
-
Homo sapiens GCN5
-
enzyme activity is regulated by phosphorylation and interaction with other regulating protein factors
-
-
-
additional information
?
-
Mus musculus C57BL/6
-
specific role of MOZ-driven acetylation in controlling a desirable balance between proliferation and differentiation during hematopoiesis. MOZ also shows activity either as Runx1 coactivator or in the induction of leukemic transformation via transcriptional intermediary factor 2, TIF2, but is not essentially required
-
-
-
additional information
?
-
Danio rerio zMoz
-
a homologue of Moz, zMoz, occurs in zebrafish to perform a potential Moz function in the trunk region
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
Ca2+ required at low concentration (5 mM), inhibition at 10-20 mM
Mg2+
-
Mg2+ required at low concentration (5 mM), inhibition at 10-20 mM
NaCl
-
10 mM: 100% activity, 100 mM: 50% of activity maximum, 250 mM: less than 10% of activity maximum
NaCl
-
7fold decrease in activity between 0.15 and 1 M
additional information
-
addition of Mg2+ and Mn2+ has no effect on activity
additional information
-
the enzyme does not bind Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2R,3S)-4-methylidene-5-oxo-2-propyltetrahydrofuran-3-carboxylic acid
-
i.e. butyrolactone MB-3, a GCN5 inhibitor
(NH4)2SO4
-
competitive against both acetyl-CoA and histones
1,7-bis(3-bromo-4-hydroxyphenyl)-1,6-heptadiene-3,5-dione
-
-
1-(S-coenzyme A)hex-6-ene
-
-
-
1-(S-coenzyme A)propan-2-one
-
-
-
2,5-diphenylisothiazol-3(2H)-one
-
-
2,6-bis(3-bromo-4-hydroxybenzylidene)cyclohexanone
-
-
2-(2-pyridyl)-isothiazol-3(2H)-one
-
-
2-(3-chloro-4-fluorophenyl)isothiazol-3(2H)-one
-
-
2-(3-methoxyphenyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
antiproliferative effects in cancer cells, GI50 value 0.007 mM with SK-N-SH cell
2-(3-pyridyl)-isothiazol-3(2H)-one
-
-
2-(4-(trifluoromethyl)benzyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
antiproliferative effects in cancer cells, GI50 value 0.0005 mM with SK-N-SH cell, 0.030 mM with MCF-7 cell
2-(4-dimethylaminoaniline)-isothiazol-3(2H)-one
-
-
2-(4-fluorophenyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
antiproliferative effects in cancer cells, GI50 value 0.010 mM with SK-N-SH cell, 0.037 mM with MCF-7 cell
2-(4-methylphenyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
antiproliferative effects in cancer cells, GI50 value 0.006 mM with SK-N-SH cell
2-(4-morpholinoaniline)-isothiazol-3(2H)-one
-
-
2-(4-pyridyl)-isothiazol-3(2H)-one
-
-
2-(phenyl)-isothiazolo[5,4-b]pyridin-3(2H)-one
-
-
2-(S-coenzyme A)acetaldehyde
-
-
2-(S-coenzyme A)acetic acid
-
-
2-(S-coenzyme A)acetic acid thiophenyl ester
-
-
2-decyl-4-hydroxyquinoline-3-carboxylic acid
-
-
2-dodecylmalonate
-
compound induces hyperacetylation of specific lysine residues of histone H3, in particular residues K9 and K18, and raises the level of pan-acetylated H4. Compound inhibits the acetylation of all the other H3 and H4 lysines, with the exception of residue K8Ac of histone H4
2-ethylisothiazol-3(2H)-one
-
-
2-pentylisothiazol-3(2H)-one
-
-
2-phenyl-5-(trityloxymethyl)isothiazol-3(2H)-one
-
-
2-phenylisothiazol-3(2H)-one
-
-
2-tert-butyl-5-(dodecylthio)isothiazol-3(2H)-one-1-oxide
-
-
2-tert-butyl-5-chloroisothiazol-3(2H)-one 1-oxide
-
-
2-tridecylmalonate
-
inhibition of the acetylation of histone H3 residuesK9/K18, being practically inactive in all the other assays
2-undecylmalonate
-
compound exhibits a significant inhibition of the acetylation of almost any lysine residue explored, with the sole exception of residue K8 of H4. Compound reduces the level of K5Ac of H4 and, more markedly, K16Ac of H4
3-(Z)-(benzylsulfanyl)propenoic acid
-
-
3-(Z)-(benzylsulfinyl)-2-N-(4-dimethylaminoanilino)-propenamide
-
-
3-(Z)-(benzylsulfinyl)-2-N-(4-morpholinoanilino)-propenamide
-
-
3-(Z)-(benzylsulfinyl)-N-(2-pyridyl)propenamide
-
-
3-(Z)-(benzylsulfinyl)-N-(3-pyridyl)propenamide
-
-
3-(Z)-(benzylsulfinyl)-N-(4-pyridyl)propenamide
-
-
3-pentadecylidenepentane-2,4-dione
-
inhibition of the acetylation of histone H3 residuesK9/K14, being practically inactive in all the other assays
3-quinolinecarboxylic acid ethyl ester
-
effects in vivo, overview
3-quinolinecarboxylic acid ethyl ester
-
effects in vivo, inhibitory effect on the transcription is not fully GCN5-specific, overview
3-Z-benzylsulfanyl-4-trityloxy-but-2-enoic acid phenylamide
-
-
3-Z-benzylsulfinyl-4-trityloxy-but-2-enoic acid phenylamide
-
-
4,5-dichloro-2-ethylisothiazol-3(2H)-one
-
-
4,5-dichloro-2-ethylisothiazol-3(2H)-one-1-oxide
-
-
4-azidomethyl-2-phenyl-isothiazol-3(2H)-one
-
-
4-bromomethyl-2-phenylisothiazol-3(2H)-one
-
-
4-hydroxy-2-methylquinoline-3-carboxylic acid
-
-
4-hydroxy-2-pentadecylquinoline-3-carboxylic acid
-
-
4-hydroxy-2-pentylquinoline-3-carboxylate
-
effects in vivo, overview, in vitro clear reduction of the acetylation extents of both histone H3 and alpha-tubulin at 0.1 mM
4-hydroxy-2-pentylquinoline-3-carboxylic acid
-
-
4-hydroxy-2-pentylquinoline-3-carboxylic acid
-
-
4-methoxymethyl-2-phenyl-isothiazol-3(2H)-one
-
-
4-methyl-2-phenylisothiazol-3(2H)-one
-
-
4-methyl-5-methoxy-2-phenyl-isothiazol-3(2H)-one
-
-
4-trityloxy-but-2-ynoic acid phenylamide
-
-
5-acetoxymethyl-2-phenylisothiazol-3(2H)-one
-
-
5-azidomethyl-2-phenylisothiazol-3(2H)-one
-
-
5-chloro-2-(3-chloro-4-fluorophenyl)isothiazol-3(2H)-one
-
-
5-chloro-2-ethyl-4-methylisothiazol-3(2H)-one
-
-
5-chloro-2-ethyl-4-methylisothiazol-3(2H)-one-1-oxide
-
-
5-chloro-2-ethylisothiazol-3(2H)-one
-
-
5-chloro-2-ethylisothiazol-3(2H)-one-1-oxide
-
-
5-chloro-2-pentylisothiazol-3(2H)-one
-
-
5-chloro-4-methyl-2-phenylisothiazol-3(2H)-one
-
-
5-hydroxymethyl-2-phenylisothiazol-3(2H)-one
-
-
5-methyl-2-phenylisothiazol-3(2H)-one
-
-
5-phenylureidomethyl-2-phenylisothiazol-3(2H)-one
-
-
acetylated histone H3 peptide
-
acetylated at Lys14, product inhibition of PCAF protein, noncompetitive against both substrates
-
acetylated histone H3 peptide
-
noncompetitive versus acetyl-CoA and histone H3
-
allspice hot water extract
-
leads to a potent anti-HAT activity since the allspice hot water extract possesses a strong inhibitory effect on p300 and CBP (40% at 0.1 ng/ml). Chromatin immunoprecipitation indicates that the acetylation of histone H3 in the PSA and B2M promoter regions was also repressed
-
anacardic acid
-
-
anacardic acid
-
anarcardic acid supresses TNF-induced HAT activity
anacardic acid
-
reversibly and noncompetitively inhibits HAT activity with a 50% inhibitory concentration of 0.03 nM. The parasiticidal effect of anacardic acid is at least partially associated with its inhibition of PfGCN5 HAT, resulting in the disturbance of the transcription program in the parasites
anacardic acid
-
-
anacardic acid
-
10 microM inhibit histidine-tagged recombinant p300 with purified human HeLa core histone as substrate by about 95%
anacardic acid
-
a p300HAT inhibitor
benzyl [2-(5-chloro-3-oxoisothiazol-2(3H)-yl)ethyl]carbamate
-
-
bisubstrate analogue histone H3-peptide-coenzyme A
-
potent inhibitor, competitive versus acetyl-CoA, non-competitive versus histone H3-peptide
-
bisubstrate analogue histone H3-peptide-coenzyme A
-
peptide consisting of 20 and 7 amino acid residues
-
bisubstrate analogue histone H3-peptide-coenzyme A
-
-
-
bisubstrate analogue methyl-histone H3-peptide-coenzyme A
-
peptide consisting of 20 and 7 amino acid residues
-
Brij-58
-
no inhibition
Ca2+
-
Mg2+ or Ca2+ required at low concentration of 5 mM, inhibition at 10-20 mM
CCT004463
-
in vivo cell proliferation inhibition
CCT004464
-
in vivo cell proliferation inhibition
CCT004465
-
in vivo cell proliferation inhibition
CCT004466
-
in vivo cell proliferation inhibition
CCT004467
-
in vivo cell proliferation inhibition
CCT077791
-
IC50: 0.0022-0.0073 mM, in vivo cell proliferation inhibition, reduces acetylation of histones H3 and H4 and alpha-tubulin in cancer cell lines
CCT077792
-
IC50: 0.0027-0.015 mM, in vivo cell proliferation inhibition
CCT077796
-
IC50: 0.0187-0.0202 mM, in vivo cell proliferation inhibition
CCT079769
-
IC50: 0.0547 mM, in vivo cell proliferation inhibition
chondroitin sulfate
-
-
CoA
-
reversible
CoA
-
competitive
CoA
-
product inhibition of PCAF protein, competitive against acetyl-CoA
CoA
-
competitive versus acetyl-CoA, Gnc5 protein
CoA
Q07794
CoA binds competitively with acetyl-CoA
Cu2+
-
5 mM, enzyme form B
curcumin
-
-
curcumin
-
-
curcumin
Herpes simplex virus
-
curcumin affects VP16-mediated recruitment of RNA polymerase II to IE gene promoters by a mechanism independent of p300/CBP histone acetyltransferase activity
curcumin
-
increase in eNOS mRNA, caused by shear stress, is completely blocked by p300 small interfering RNA; increase in eNOS mRNA, caused by shear stress, is completely blocked by pharmacological inhibition of p300/HAT activity with curcumin
curcumin
-
25 microM inhibit histidine-tagged recombinant p300 with purified human HeLa core histone as substrate by about 75%
desulfo-coenzyme A
-
dead-end inhibitor, noncompetitive versus histone H3-peptide and competitive versus actyl-CoA
desulfo-coenzyme A
-
dead-end inhibitor, competitive versus acetyl-CoA, Gcn5 protein
diethyl 2-tetradecylidenemalonate
-
compound exhibits a significant inhibition of the acetylation of almost any lysine residue explored, with the sole exception of residue K8 of H4. Compound reduces the level of K5Ac of H4 and, more markedly, K16Ac of H4
dimethyl sulfoxide
-
irreversible at 2% v/v
dithiothreitol
-
10 mM: stimulation, 100 mM: inhibition
DNA
-
added DNA forming complexes with the histones inhibits activity
DNA
-
in vitro, enzyme form A inhibited, enzyme form B relatively insensitive
DNA
-
enzyme form A activated by low concentrations, enzyme form B inhibited
DNA
-
acetylation of histone H1by the enzymes PCAF and GNC5 is inhibited in vivo by complexing of H1 with DNA
EDTA
-
weak
EDTA
-
high concentrations
ethanol
-
irreversible at 2% v/v
ethyl 2-decyl-4-hydroxyquinoline-3-carboxylate
-
-
ethyl 2-methylquinoline-3-carboxylate
-
effects in vivo, overview
ethyl 2-methylquinoline-3-carboxylate
-
effects in vivo, inhibitory effect on the transcription is fully GCN5-specific, overview
ethyl 3-(5-chloro-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
ethyl 4-hydroxy-2-methylquinoline-3-carboxylate
-
-
ethyl 4-hydroxy-2-pentadecylquinoline-3-carboxylate
-
-
ethyl 4-hydroxy-2-pentylquinoline-3-carboxylate
-
-
Fe2+
-
5 mM, enzyme form B
garcinol
-
10 microM inhibit histidine-tagged recombinant p300 with purified human HeLa core histone as substrate by about 80%
garcinol
-
competitive inhibitor versus both acetyl-CoA and histone, docking to the p300 HAT domain encompasses amino acid residues 1284-1673, and inhibition mechanism, overview. Also inhibits PCAF HAT activity, 90% inhibition at 0.04 mM
garcinol
-
both the acetylation and induction of the inflammatory proteins in elevated glucose levels are significantly inhibited by inhibitors of histone acetyltransferase, such as garcinol and antisense against the histone acetylase, p300
geminin
-
a Cdt1 repressor, inhibits HBO1 acetylase activity in a a Cdt1-dependent manner in the context of a Cdt1-HBO1 complex, and it associates with origins and inhibits H4 acetylation and licensing in vivo, but geminin does not block the interaction of Cdt1 with HBO1 in vitro or Cdt1-dependent recruitment of HBO1 to replication origins in vivo
-
H3-CoA-20
-
IC50: 0.034-0.064 mM
-
H3-CoA-20-Tat
-
IC50: 0.012 mM, recombinant enzyme
-
HC toxin
Q8LPU4
cyclic tetrapeptide, decreases enzyme form B expression
heparan sulfate proteoglycans
-
heparan sulfate proteoglycans isolated from corneal and pulmonary fibroblasts inhibit HAT activity with similar effectiveness as heparin
-
heparin
-
ability of heparin to inhibit HAT is dependent upon its size and structure: small heparin-derived oligosaccharides (above 8 sugars) and N-desulfated or O-desulfated heparin show reduced inhibitory activity. Heparin is shown to bind to pCAF. Enzyme assays indicate that heparin shows the characteristics of a competitive-like inhibitor causing a 50fold increase in the Km of pCAF for histone H4
histone
-
inhibits spermidine acetylation, enzyme forms A and B
histone
-
histones H2A, H2B, and H3
histone
-
histone H1 acetylation is inhibited by all other histone fractions
histone H3-peptide mutant K14A
-
dead-end inhibitor analogue, mutant histone H3 -peptide consisting of amino acid residues 3-20 K14A
-
hyaluronic acid
-
-
iodoacetamide
-
-
iodoacetamide
-
enzyme form A is more sensitive than enzyme form B
isogarcinol
-
10 microM inhibit histidine-tagged recombinant p300 with purified human HeLa core histone as substrate by about 70%
isogarcinol
-
competitive inhibitor versus both acetyl-CoA and histone, docking to the p300 HAT domain encompasses amino acid residues 1284-1673, and inhibition mechanism, overview. Also inhibits PCAF HAT activity to maximally 50%
Isopropanol
-
irreversible at 2% v/v
isothiazolone
-
binds irreversibly to proteins via thiol interactions
K+
-
175 mM: 50% inhibition
K+
-
strong
Keratan sulfate
-
-
LTK14
-
20 microM inhibit histidine-tagged recombinant p300 with purified human HeLa core histone as substrate by about 70%
LTK14
-
noncompetitive inhibitor versus both acetyl-CoA and histone, docking to the p300 HAT domain encompasses amino acid residues 1284-1673, and inhibition mechanism, overview
Lys-CoA
-
autoacetylation IC50: 100 nM, below
Lys-CoA
-
IC50: 310-420 nM
Lys-CoA-Tat
-
IC50: 250 nM, recombinant enzyme, complete inhibtion of acetylation of the promyelotic leukemia zinc finger gene
methyl 2-(5-chloro-3-oxoisothiazol-2(3H)-yl)ethanoate
-
-
methyl 3-(3-oxoisothiazol-2(3H)-yl)propanoate
-
-
methyl 3-(4,5-dichloro-1-oxido-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
methyl 3-(4,5-dichloro-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
methyl 3-(5-chloro-1-oxido-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
methyl 3-(5-chloro-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
methyl 3-(5-chloro-3-oxoisothiazol-2(3H)-yl)propanoic acid
-
-
methyl 3-(5-chloro-4-methyl-1-oxido-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
methyl 3-(5-chloro-4-methyl-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
methyl 3-[(5-chloroisothiazol-3-yl)amino]propanoate
-
-
methyl 3-[4-chloro-5-(dodecylthio)-1-oxido-3-oxoisothiazol-2(3H)-yl]propanoate
-
-
methyl 3-[4-{[(benzyloxy)carbonyl]amino}-5-chloro-3-oxoisothiazol-2(3H)-yl]propanoate
-
-
methyl 3-[5-(dodecylthio)-1-oxido-3-oxoisothiazol-2(3H)-yl] propanoate
-
-
methyl 4-(3-oxoisothiazol-2(3H)-yl)butanoate
-
-
methyl 4-(5-chloro-3-oxoisothiazol-2(3H)-yl)butanoate
-
-
methyl 5-(5-chloro-3-oxoisothiazol-2(3H)-yl)pentanoate
-
-
methyl 6-(5-chloro-3-oxoisothiazol-2(3H)-yl)hexanoate
-
-
Mg2+
-
no inhibition
Mg2+
-
37 mM: 50% inhibition
Mg2+
-
Mg2+ or Ca2+ required at low concentration, 5 mM, inhibition at 10-20 mM
Mn2+
-
no inhibition
N-(4-cyano-3-(trifluoromethyl)phenyl)-2-decyl-6-ethoxybenzamide
-
inhibits human p300 recombinant enzyme similar to anacardic acid
N-(4-cyano-3-(trifluoromethyl)phenyl)-2-ethoxy-6-octylbenzamide
-
inhibits human p300 recombinant enzyme similar to anacardic acid, moreover this inhibitor induces significant apoptosis at 0.05 nM in U937 leukemia cells
N-ethylmaleimide
-
-
N-ethylmaleimide
-
-
N-ethylmaleimide
-
enzyme form A is more sensitive than enzyme form B
N-ethylmaleimide
-
-
N-[2-(S-Coenzyme A)acetyl]spermidine amide
-
strong
-
N6-Acetyllysine
-
competitive
Na+
-
160 mM: 50% inhibition
Na+
-
competitive against both acetyl-CoA and histones
Na+
-
strong
p-chloromercuribenzoate
-
strongly inhibits activity of enzyme form B, formation of acyl-enzyme complex
p-chloromercuribenzoate
-
enzyme form B less sensitive than enzyme form A
p-chloromercuribenzoate
-
-
Plumbagin
-
RTK1, naturally occurring hydroxynaphthoquinone, isolated from Plumbago rosea roots, inhibits histone acetylation, and induces apoptosis at higher concentrations, it inhibits p300/CBP-mediated acetylation of p53 lysine 373 non-competitively, 25 microM inhibit histidine-tagged recombinant p300 with purified human HeLa core histone as substrate by about 60% compared to control; RTK1, naturally occurring hydroxynaphthoquinone, isolated from Plumbago rosea roots, it does not inhibit PCAF acetylation of p53 lysine 320 in vivo in HEK-293 cells (pretreated with acetylation inducer doxorubicin), but 10, 25, and 50 microM inhibit FLAG-tagged recombinant PCAF in vitro (30C) with purified human HeLa core histone as substrate
Plumbagin
-
RTK1, naturally occurring hydroxynaphthoquinone, isolated from Plumbago rosea roots
Polyarginine
-
-
Polyglutamic acid
-
-
potassium phosphate
-
90 mM
Sea urchin sperm chromatin
-
-
-
siRNA
-
silencing of enzyme gene
-
spermidine
-
inhibits histone acetylation at high concentrations, enzyme forms A and B
Triton X-100
-
-
Triton X-100
-
no inhibition
Zn2+
-
5 mM, enzyme form B
montelukast
-
decreases HAT activity by attenuating the activating effect of TNF-alpha
additional information
-
monovalent cations cause a 50% decrease in activity at an average concentration of 51 mM, divalent cations at 15 mM
-
additional information
-
screening for small molecule inhibitors reducing the cell growth, overview
-
additional information
-
inhibitory potency of N-substituted isothiazolone-based compounds, in vivo cell proliferation inhibition, overview
-
additional information
-
although downregulation of UHRF1 by RNA interference enhances Tip60 expression, a significant decrease of the level of acetylated H2AK5 is observed
-
additional information
-
relative inhibition of p300, CBP, GCN5, and PCAF, overview
-
additional information
-
effects of three monofluorinated phenylalanine analogs p-fluorophenylalanine, m-fluorophenylalanine, and o-fluorophenylalanine on the stability and enzymatic activity of tGCN5, overview
-
additional information
-
5-chloroisothiazoline inhibitors: design, synthesis and study of inhibitory potencies and inhibition of cell growth, molecular modeling, overview; N-methyl-5-chloroisothiazolone/N-methylisothiazolone in a ratio of 3:1 in Kathon TM CG, a preservative in cosmetics, that inhibits PCAF and the growth of cell lines A2780 and HEK-293
-
additional information
-
protein factors such as E1A and Nap1 can modulate p300/CBP HAT activity
-
additional information
-
inhibitory potencies of isothiazolones and isothiazolone-1-oxides on PCAF and growth inhibition of Hep-G2 cancer cells, overview
-
additional information
-
HBO1 dissociation from origins is either triggered by proteolytic degradation of a key licensing cofactor or by post-translational event(s) induced by HU and/or actinomycin D treatment(s)
-
additional information
-
no inhibition of p300 by 5-methoxy-2-methyl-1,4-naphthoquinone (RTK2, alkyl substitution of hydroxyl group), 5-ethoxy-2-methyl-1,4-naphthoquinone (RTK3, alkyl substitution of hydroxyl group), 5-isopropoxy-2-methyl-1,4-naphthoquinone (RTK4, alkyl substitution of hydroxyl group), and 5-[2-(dimethylamino)-ethoxy]-2-methyl-1,4-naphthoquinone (RTK10, N,N-dimethylamine substitution of hydroxyl group), less than 10% inhibition with 6-methyl-5,8-dioxo-5,8-dihydronaphthalen-1-yl acetate (RTK5, acetyl substitution of hydroxyl group), 6-methyl-5,8-dioxo-5,8-dihydronaphthalen-1-yl methanesulfonate (RTK6, sulfonyl substitution of hydroxyl group), 2-methyl-5-(2-piperidin-1-ylethoxy)-1,4-naphthoquinone (RTK7, piperidine substitution of hydroxyl group), 2-methyl-5-(2-morpholin-4-ylethoxy)-1,4-naphthoquinone (RTK8, morpholine substitution of hydroxyl group), and ethyl [(6-methyl-5,8-dioxo-5,8-dihydronaphthalen-1-yl)-oxy]acetate (RTK9, ester substitution of hydroxyl group)
-
additional information
-
effect of garcinol and its derivative on PCAF stability, overview. Isothermal titration calorimetry studies and molecular mechanisms of p300 HAT inhibition by specific and nonspecific HAT inhibitors: garcinol, isogarcinol. Residues LTK14, S1396, Y1397, G1626, and R1627 contact the inhibitors, overview
-
additional information
-
PPARgamma suppresses eotaxin, an NF-kappaB target, gene expression by direct inhibition of p65-associated HAT activity, for example, by competing with p65 for limited amounts of CBP and/or by recruiting HDAC to the p65-HAT complex
-
additional information
-
both the acetylation and induction of the inflammatory proteins in elevated glucose levels are significantly inhibited by inhibitors of histone acetyltransferase, such as garcinol and antisense against the histone acetylase, p300
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-chloro-4-trifluoromethyl benzamide
-
activates the enzyme, the relative position of the -CF3 and -Cl is crucial for p300 HAT activity enhancement, overview
2-dodecylmalonate
-
compound induces hyperacetylation of specific lysine residues of histone H3, in particular residues K9 and K18, and raises the level of pan-acetylated H4. Compound inhibits the acetylation of all the other H3 and H4 lysines, with the exception of residue K8Ac of histone H4
4-chloro-2-trifluoromethyl benzamide
-
activates the enzyme, the relative position of the -CF3 and -Cl is crucial for p300 HAT activity enhancement, overview
Asf1
-
a histone chaperone that activates the enzyme
-
Bleomycin
-
activation of the ATM-associated enzyme by bleomycin, the free, non-ATM-associated, Tip60 activity is not activated by bleomycin
CDT1
-
licensing factor Cdt1 stabilizes HBO1 at origins. HBO1 directly interacts with Cdt1, and it enhances Cdt1-dependent rereplication
-
diethyl 2-pentadecylidenemalonate
-
compound induces hyperacetylation of specific lysine residues of histone H3, in particular residues K9 and K18, and raises the level of pan-acetylated H4
dimethyl-arginine 3-histone H4
-
dimethyl H4R3 provides a binding surface for PCAF and directly enhances histone H3 and H4 acetylation in vitro
-
dithiothreitol
-
10 mM: stimulation, 100 mM: inhibition
DNA
-
enzyme form A activated by low concentration, enzyme form B inhibited
ethanol
-
ethanol induces the enzyme expression in the liver and activates the enzyme at specific lysine residues, e.g. Lys9 or Lys14, mechanisms of ethanol-induced histone H3 acetylation, overview
ethanol
-
induces and activates the enzyme and histone acetylation
IKKalpha
-
after cytokine stimulation, IKKalpha also phosphorylates CBP at Ser1382 and Ser1386, thus enhancing CBP-binding affinity for p65 and CBP HAT activity and, consequently, histone H3 acetylation
-
laminar shear stress
-
laminar shear stress stimulates acetylation of histones 3 and 4 at the region of the eNOS promoter SSRE and extends 3' toward the eNOS coding region. Laminar shear stress induces p300 binding to p65 and leads to increase of p300 histone acetyltransferase activity by 2.5fold and to increase of acetylation of p65, but not of p50 acetylation, overview
-
membrane transporter
-
for acetyl-CoA and acetyltransferase from cytosol into lumen of the ER/ER Golgi intermediate compartment
-
NuA4
-
cooperation with the NuA4 complex to enhance its functions but independent contribution to acetylation
-
p65
-
p65-associated HAT activity. TNFalpha-dependent phosphorylation of p65 at either Ser276 or Ser536 increases its interaction with p300 and its subsequent acetylation at Lys310 of the NFkappaB subunit, indicating that p65 phosphorylation regulates subsequent acetylation
-
SAGA
-
cooperation with the Gcn5 complex to enhance its functions but independent contribution to acetylation
-
Sas4
-
absolutely required for acetylation activity of Sas2 in SAS complex
-
Sas5
-
stimulates acetylation activity of Sas2 in SAS complex
-
spermine
Artemia nauplii
-
activates acetylation of H3
TNF-alpha
-
-
-
TNF-alpha
-
a significant induction of NF-kB p65-associated HAT activity by TNF-alpha is demonstrated, which is attenuated by the presence of montelukast in a concentration-dependent manner
-
VPS75
-
Rtt109's H3-K9 acetylation activity in vitro is enhanced strongly by histone chaperone Vps75, Asf1, and Vps75 are both required for acetylation of Lys9 on H3 in vivo by Rtt109, whereas acetylation of Lys56 on H3 in vivo requires only Asf1
-
VPS75
-
histone chaperone, acts as activating subunit
-
Vps75/Asf1
-
helper proteins required by Rtt109 for full catalytic activity. Stimulation of Rtt109 activity by Vps75 results in 50fold increase of the kcat value with unaltered Km
-
Yng2/Epl1
-
helper proteins required by Esa1 for full catalytic activity. Esa1 minimally requires Yng2 and Epl1 for full catalytic activity and nucleosome recognition
-
methylated histone
-
stimulates interaction of NuA4 complex with histone
additional information
-
requires free SH-groups
-
additional information
-
phosphorylation of histone H3 enhances Gcn5 enzyme activity
-
additional information
-
no activation by 3-chloro-2-trifluoromethyl benzamide and 2-chloro-3-trifluoromethyl benzamide
-
additional information
-
bombesin induces p300 expression 2fold, as part of the Src and PKCdelta pathways, a neutral endopeptidase, which degrades bombesin, or bombesin receptor antagonists, or p300 siRNA block bombesin-induced p300 HAT activity, overview
-
additional information
-
DNA damage induces the rapid acetylation of ATM by Tip60
-
additional information
-
HATs form multisubunit complexes, and HAT interacting partners appear to regulate HAT activity by altering substrate specificity, targeting to specific loci, enhancing acetyltransferase activity, restricting access of non-target proteins, and coordinating the multiple enzyme activities of the complex
-
additional information
-
protein factors such as E1A and Nap1 can modulate p300/CBP HAT activity. Rtt109 requires a histone chaperone for efficient catalysis. HATs form multisubunit complexes, and HAT interacting partners appear to regulate HAT activity by altering substrate specificity, targeting to specific loci, enhancing acetyltransferase activity, restricting access of non-target proteins, and coordinating the multiple enzyme activities of the complex
-
additional information
-
Hat1 and Hat2, but not Hif1, of the HAT-B complex are required for the Lys12/Lys5-specific acetylation and for histone H4 binding
-
additional information
-
HBO1 interacts both with transcriptional activator proteins and with MCM2 and ORC1
-
additional information
-
partial loop deletion or autoacetylation of up to 17 sites in p300 HAT leads to an increase in catalytic activity by 4-10fold
-
additional information
-
coexpression of HBO1 and Jade-1 increases histone acetylation and MCM complex loading, loading, whereas overexpression of HBO1 alone does not
-
additional information
-
CBP is activated by phosphorylation at Ser1382 and Ser1386
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0002
acetyl-CoA
-
enzyme form B
0.00023
acetyl-CoA
-
enzyme form DB
0.00027
acetyl-CoA
-
pH 7.5, 25C, mutants D639E and L503P/D601G/Y612C, with substrate histone H3
0.00028
acetyl-CoA
-
pH 7.5, 25C, wild-type P/CAF enzymem, with substrate histone H3
0.00029
acetyl-CoA
-
pH 7.5, 25C, mutant Y612C/P655R, with substrate histone H3
0.0003
acetyl-CoA
Q07794
wild-type, pH 7.5, 25C
0.00033
acetyl-CoA
-
pH 7.5, 25C, mutant L503P, with substrate histone H3
0.00034
acetyl-CoA
-
pH 7.5, 25C, mutants D601G and V582A/D639E, with substrate histone H3
0.00036
acetyl-CoA
-
pH 7.5, 25C, mutant V582A, with substrate histone H3
0.00041
acetyl-CoA
-
pH 7.5, 25C, mutant Y612C, with substrate histone H3
0.00049
acetyl-CoA
Q07794
mutant D288N, pH 7.5, 25C
0.00058
acetyl-CoA
-
Gcn5
0.00062
acetyl-CoA
-
Gcn5
0.0007
acetyl-CoA
-
Gcn5 mutant A190T
0.0007
acetyl-CoA
Q07794
mutant D287N, pH 7.5, 25C
0.0009
acetyl-CoA
-
-
0.00098
acetyl-CoA
-
PCAF protein
0.0012
acetyl-CoA
-
pH 7.5, 25C, mutant E338Q
0.0015
acetyl-CoA
-
pH 7.5, 25C, mutant C304A
0.0016
acetyl-CoA
-
recombinant PCAF catalytic domain mutant Y638A; recombinant wild-type PCAF catalytic domain
0.002
acetyl-CoA
-
pH 7.5, 25C, mutant C304S
0.0021
acetyl-CoA
-
Gcn5 mutant A190S
0.0023
acetyl-CoA
-
wild-type, presence of 0.4 equivalents of chaperone Vps75, pH 7.5, 25C
0.0025
acetyl-CoA
-
Gcn5 protein
0.0025
acetyl-CoA
-
pH 7.5, 25C, wild-type enzyme
0.0033
acetyl-CoA
-
autoacetylation
0.0039
acetyl-CoA
-
wild-type, presence of 0.2 equivalents of chaperone Vps75, pH 7.5, 25C
0.0063
acetyl-CoA
-
wild-type, presence of 1 equivalent of chaperone Vps75, pH 7.5, 25C
0.0065
acetyl-CoA
-
wild-type, presence of 2 equivalents of chaperone Vps75, pH 7.5, 25C; wild-type, presence of 8 equivalents of chaperone Vps75, pH 7.5, 25C
0.0067
acetyl-CoA
O14929
wild-type, pH not specified in the publication, temperature not specified in the publication
0.009
acetyl-CoA
-
-
0.01
acetyl-CoA
-
+ spermidine, enzyme form B
0.015
acetyl-CoA
-
+ spermidine, enzyme form A
0.046
acetyl-CoA
-
PCAF catalytic domain
0.007
Histone H3
Q07794
wild-type, pH 7.5, 25C
-
0.039
Histone H3
-
pH 7.5, 25C, mutant D639E
-
0.053
Histone H3
-
pH 7.5, 25C, wild-type P/CAF enzyme
-
0.054
Histone H3
-
pH 7.5, 25C, mutant L503P
-
0.055
Histone H3
-
pH 7.5, 25C, mutant D601G
-
0.062
Histone H3
-
pH 7.5, 25C, mutant Y612C/P655R
-
0.064
Histone H3
-
pH 7.5, 25C, mutant L503P/D601G/Y612C
-
0.165
Histone H3
-
pH 7.5, 25C, mutant Y612C
-
0.24
Histone H3
Q09472
wild-type, pH 8.0, temperature not specified in the publication
-
0.273
Histone H3
-
Gcn5 mutant A190S
-
0.352
Histone H3
-
Gcn5 mutant A190T
-
0.357
Histone H3
-
Gcn5
-
0.43
Histone H3
Q09472
mutant having m-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication
-
0.46
Histone H3
-
pH 7.5, 25C, mutant V582A/D639E
-
0.471
Histone H3
-
Gcn5
-
0.49
Histone H3
-
Gcn5
-
0.532
Histone H3
-
PCAF
-
0.56
Histone H3
-
pH 7.5, 25C, mutant V582A
-
0.69
Histone H3
-
recombinant p-fluorophenylalanine-substituted tGCN5
-
0.7
Histone H3
Q09472
mutant having p-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication
-
0.8
Histone H3
-
recombinant o-fluorophenylalanine-substituted wild-type tGCN5
-
1.05
Histone H3
-
recombinant wild-type tGCN5
-
2.09
Histone H3
-
recombinant m-fluorophenylalanine-substituted wild-type tGCN5
-
0.044
histone H3 tail peptide
Q07794
mutant D287A/D288A, pH 7.5, 25C
-
0.055
histone H3 tail peptide
Q07794
mutant D288N, pH 7.5, 25C
-
0.062
histone H3 tail peptide
Q07794
mutant D287N, pH 7.5, 25C
-
0.112
histone H3 tail peptide
Q07794
wild-type, pH 7.5, 25C
-
0.05
histone H3-peptide
-
PCAF catalytic domain
-
0.49
histone H3-peptide
-
Gcn5 protein
-
1.12
histone H3-peptide p19
-
PCAF catalytic domain
-
0.75
histone H3-peptide p27
-
PCAF catalytic domain
-
0.0000053
histone H4
-
in the absence of heparin
0.000259
histone H4
-
in the presence of heparin
0.11
histone H4
-
pH 7.5, 25C, mutant L503P/D601G/Y612C
0.12
histone H4
-
pH 7.5, 25C, mutant Y612C
0.14
histone H4
-
pH 7.5, 25C, mutant Y612C/P655R
0.15
histone H4
-
pH 7.5, 25C, mutant D639E
0.19
histone H4
-
pH 7.5, 25C, wild-type P/CAF enzyme
0.21
histone H4
-
pH 7.5, 25C, mutant L503P
0.25
histone H4
-
pH 7.5, 25C, mutant D601G
0.31
histone H4
-
pH 7.5, 25C, mutant V582A/D639E
0.5
histone H4
-
pH 7.5, 25C, mutant V582A
0.0208
histone H4 peptide
O14929
wild-type, pH not specified in the publication, temperature not specified in the publication
-
0.0595
histone H4 peptide
O14929
mutant W199A, pH not specified in the publication, temperature not specified in the publication
-
0.086
histone H4 peptide
O14929
mutant D62A, pH not specified in the publication, temperature not specified in the publication
-
0.099
histone H4 peptide
O14929
mutant D277N, pH not specified in the publication, temperature not specified in the publication
-
0.1
histone H4 peptide
O14929
mutant E276Q, pH not specified in the publication, temperature not specified in the publication
-
0.1267
histone H4 peptide
O14929
mutant E64A, pH not specified in the publication, temperature not specified in the publication
-
0.135
piccoloNuA4 peptide
-
pH 7.5, 25C, mutant E338Q
-
0.182
piccoloNuA4 peptide
-
pH 7.5, 25C, mutant C304S
-
0.216
piccoloNuA4 peptide
-
pH 7.5, 25C, wild-type enzyme
-
0.372
piccoloNuA4 peptide
-
pH 7.5, 25C, mutant C304A
-
1.28
protein p53
Q09472
mutant having p-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication
-
4.63
protein p53
Q09472
wild-type, pH 8.0, temperature not specified in the publication
-
0.18
spermidine
-
enzyme form B
0.2
spermidine
-
-
0.27
spermidine
-
enzyme form A
0.197
histone H4 peptide
O14929
mutant E187Q, pH not specified in the publication, temperature not specified in the publication
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
Gcn5 protein mutants
-
additional information
additional information
-
steady-state kinetic analyses, kinetic mechanism
-
additional information
additional information
-
kinetics, site-specific kinetic analysis of loop autoacetylation, PCAF-mediated autoacetylation, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0011
acetyl-CoA
-
mutant E374A/E378A/D301K, pH 7.5, 25C
0.0021
acetyl-CoA
-
mutant E374K/E378K, pH 7.5, 25C
0.0023
acetyl-CoA
-
wild-type, pH 7.5, 25C
0.0024
acetyl-CoA
-
mutant E374A/E378A, pH 7.5, 25C
0.0028
acetyl-CoA
Q07794
mutant D288N, pH 7.5, 25C
0.0091
acetyl-CoA
-
pH 7.5, 25C, mutant E338Q
0.0117
acetyl-CoA
-
PCAF protein
0.012
acetyl-CoA
Q07794
mutant D287N, pH 7.5, 25C
0.0267
acetyl-CoA
-
mutant A190S and A190T of Gcn5
0.0267
acetyl-CoA
-
Gcn5
0.0283
acetyl-CoA
-
Gcn5 protein
0.0283
acetyl-CoA
-
Gcn5 protein
0.031
acetyl-CoA
-
mutant E374A/E378A/D301K, presence of chaperone Vps75, pH 7.5, 25C
0.035
acetyl-CoA
-
mutant E374K/E378K, presence of chaperone Vps75, pH 7.5, 25C
0.0383
acetyl-CoA
-
PCAF catalytic domain
0.0383
acetyl-CoA
-
PCAF
0.0467
acetyl-CoA
-
PCAF catalytic domain
0.07
acetyl-CoA
-
wild-type, wild-type, presence of 0.2 equivalents of chaperone Vps75, pH 7.5, 25C
0.08
acetyl-CoA
-
wild-type, presence of 0.4 equivalents of chaperone Vps75, pH 7.5, 25C
0.11
acetyl-CoA
Q07794
wild-type, pH 7.5, 25C
0.14
acetyl-CoA
-
pH 7.5, 25C, mutant C304S
0.16
acetyl-CoA
-
mutant E374A/E378A, presence of chaperone Vps75, pH 7.5, 25C
0.28
acetyl-CoA
-
wild-type, presence of 1 equivalent of chaperone Vps75, pH 7.5, 25C
0.48
acetyl-CoA
-
wild-type, presence of 2 equivalents of chaperone Vps75, pH 7.5, 25C
0.62
acetyl-CoA
-
wild-type, presence of 8 equivalents of chaperone Vps75, pH 7.5, 25C
0.76
acetyl-CoA
-
pH 7.5, 25C, mutant C304A
0.8
acetyl-CoA
-
enzyme form type I
1.6
acetyl-CoA
-
pH 7.5, 25C, wild-type enzyme
4.14
acetyl-CoA
O14929
wild-type, pH not specified in the publication, temperature not specified in the publication
12
acetyl-CoA
-
enzyme form type II
0.8
histone
-
enzyme form type I
12
histone
-
enzyme form type II
0.0267
Histone H3
-
mutant A190S and A190T of Gcn5
-
0.0267
Histone H3
-
Gcn5
-
0.18
Histone H3
-
pH 7.5, 25C, mutants V582A and Y612C
-
0.2
Histone H3
-
pH 7.5, 25C, wild-type P/CAF enzyme, and mutants L503P, D601G, D639E, Y612C/P655R, and L503P/D601G/Y612C
-
0.22
Histone H3
-
pH 7.5, 25C, mutant V582A/D639E
-
0.62
Histone H3
Q07794
wild-type, pH 7.5, 25C
-
0.0011
histone H3 tail peptide
Q07794
mutant D287A/D288A, pH 7.5, 25C
-
0.0021
histone H3 tail peptide
Q07794
mutant D288N, pH 7.5, 25C
-
0.0087
histone H3 tail peptide
Q07794
mutant D287N, pH 7.5, 25C
-
0.11
histone H3 tail peptide
Q07794
wild-type, pH 7.5, 25C
-
0.0117
histone H3-peptide
-
PCAF protein
-
0.0283
histone H3-peptide
-
Gcn5 protein
-
0.0283
histone H3-peptide
-
Gcn5 protein
-
0.0383
histone H3-peptide
-
PCAF catalytic domain
-
0.0383
histone H3-peptide
-
PCAF
-
0.0467
histone H3-peptide
-
PCAF catalytic domain
-
0.0667
histone H3-peptide
-
PCAF catalytic domain
-
0.131
histone H3-peptide p19
-
PCAF catalytic domain
-
0.00317
histone H3-peptide p20
-
PCAF catalytic domain, recombinant mutant Y638A
-
0.0583
histone H3-peptide p20
-
PCAF catalytic domain, recombinant wild-type
-
0.17
histone H3-peptide p27
-
PCAF catalytic domain
-
0.0018
histone H4
-
pH 7.5, 25C, mutant Y612C
0.003
histone H4
-
pH 7.5, 25C, mutant L503P/D601G/Y612C
0.0035
histone H4
-
pH 7.5, 25C, mutant Y612C/P655R
0.0048
histone H4
-
pH 7.5, 25C, wild-type P/CAF enzyme and mutant V582A
0.0053
histone H4
-
pH 7.5, 25C, mutant L503P
0.0055
histone H4
-
pH 7.5, 25C, mutant D601G
0.0058
histone H4
-
pH 7.5, 25C, mutant D639E
0.0062
histone H4
-
pH 7.5, 25C, mutant V582A/D639E
0.15
histone H4 peptide
O14929
mutant E276Q, pH not specified in the publication, temperature not specified in the publication
-
0.28
histone H4 peptide
O14929
mutant E187Q, pH not specified in the publication, temperature not specified in the publication
-
0.41
histone H4 peptide
O14929
mutant W199A, pH not specified in the publication, temperature not specified in the publication
-
0.5
histone H4 peptide
O14929
mutant E64A, pH not specified in the publication, temperature not specified in the publication
-
0.53
histone H4 peptide
O14929
mutant D277N, pH not specified in the publication, temperature not specified in the publication
-
1.99
histone H4 peptide
O14929
mutant D62A, pH not specified in the publication, temperature not specified in the publication
-
4.28
histone H4 peptide
O14929
wild-type, pH not specified in the publication, temperature not specified in the publication
-
additional information
additional information
-
Gcn5 protein mutants
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.025
acetyl-CoA
Q07794
mutant D287A/D288A, pH 7.5, 25C
29
0.6
acetyl-CoA
Q07794
mutant D288N, pH 7.5, 25C
29
17
acetyl-CoA
Q07794
mutant D287N, pH 7.5, 25C
29
18
acetyl-CoA
-
wild-type, presence of 0.2 equivalents of chaperone Vps75, pH 7.5, 25C
29
35
acetyl-CoA
-
wild-type, presence of 0.4 equivalents of chaperone Vps75, pH 7.5, 25C
29
44
acetyl-CoA
-
wild-type, presence of 1 equivalent of chaperone Vps75, pH 7.5, 25C
29
73
acetyl-CoA
-
wild-type, presence of 2 equivalents of chaperone Vps75, pH 7.5, 25C
29
97
acetyl-CoA
-
wild-type, presence of 8 equivalents of chaperone Vps75, pH 7.5, 25C
29
390
acetyl-CoA
Q07794
wild-type, pH 7.5, 25C
29
619
acetyl-CoA
O14929
wild-type, pH not specified in the publication, temperature not specified in the publication
29
0.053
Histone H3
-
recombinant o-fluorophenylalanine-substituted tGCN5
0
0.14
Histone H3
-
recombinant p-fluorophenylalanine-substituted tGCN5
0
0.25
Histone H3
-
recombinant m-fluorophenylalanine-substituted tGCN5
0
0.87
Histone H3
-
recombinant wild-type tGCN5
0
2.73
Histone H3
Q09472
mutant having p-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication
0
7.48
Histone H3
Q09472
mutant having m-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication
0
9.73
Histone H3
Q09472
wild-type, pH 8.0, temperature not specified in the publication
0
90
Histone H3
Q07794
wild-type, pH 7.5, 25C
0
0.025
histone H3 tail peptide
Q07794
mutant D287A/D288A, pH 7.5, 25C
0
0.038
histone H3 tail peptide
Q07794
mutant D288N, pH 7.5, 25C
0
0.14
histone H3 tail peptide
Q07794
mutant D287N, pH 7.5, 25C
0
0.98
histone H3 tail peptide
Q07794
wild-type, pH 7.5, 25C
0
1.41
histone H4 peptide
O14929
mutant E187Q, pH not specified in the publication, temperature not specified in the publication
0
1.5
histone H4 peptide
O14929
mutant E276Q, pH not specified in the publication, temperature not specified in the publication
0
3.95
histone H4 peptide
O14929
mutant E64A, pH not specified in the publication, temperature not specified in the publication
0
4.88
histone H4 peptide
O14929
mutant W199A, pH not specified in the publication, temperature not specified in the publication
0
5.36
histone H4 peptide
O14929
mutant D277N, pH not specified in the publication, temperature not specified in the publication
0
23.9
histone H4 peptide
O14929
mutant D62A, pH not specified in the publication, temperature not specified in the publication
0
205
histone H4 peptide
O14929
wild-type, pH not specified in the publication, temperature not specified in the publication
0
0.036
protein p53
Q09472
wild-type, pH 8.0, temperature not specified in the publication
0
0.068
protein p53
Q09472
mutant having p-fluorophenylalanine incorporated, pH 8.0, temperature not specified in the publication
0
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
8
(NH4)2SO4
-
versus acetyl-CoA
12
(NH4)2SO4
-
versus histone
0.000014
1-(S-coenzyme A)hex-6-ene
-
-
-
0.0198
2,5-diphenylisothiazol-3(2H)-one
-
PCAF
0.019
2-(2-pyridyl)-isothiazol-3(2H)-one
-
PCAF
0.028
2-(2-pyridyl)-isothiazol-3(2H)-one
-
p300
0.0065
2-(3-pyridyl)-isothiazol-3(2H)-one
-
PCAF
0.0144
2-(3-pyridyl)-isothiazol-3(2H)-one
-
p300
0.0054
2-(4-dimethylaminoaniline)-isothiazol-3(2H)-one
-
PCAF
0.0075
2-(4-morpholinoaniline)-isothiazol-3(2H)-one
-
PCAF
0.0099
2-(4-morpholinoaniline)-isothiazol-3(2H)-one
-
p300
0.0015
2-(4-pyridyl)-isothiazol-3(2H)-one
-
PCAF
0.003
2-(4-pyridyl)-isothiazol-3(2H)-one
-
p300
0.032
2-(S-coenzyme A)acetic acid
-
-
0.0013
2-(S-coenzyme A)acetic acid thiophenyl ester
-
-
0.023
2-phenylisothiazol-3(2H)-one
-
p300
0.028
2-phenylisothiazol-3(2H)-one
-
PCAF
0.0427
4-azidomethyl-2-phenyl-isothiazol-3(2H)-one
-
PCAF
0.102
4-methoxymethyl-2-phenyl-isothiazol-3(2H)-one
-
PCAF
0.032
4-methyl-2-phenylisothiazol-3(2H)-one
-
PCAF
0.2
4-methyl-5-methoxy-2-phenyl-isothiazol-3(2H)-one
-
above, PCAF
0.162
5-acetoxymethyl-2-phenylisothiazol-3(2H)-one
-
PCAF
0.058
5-azidomethyl-2-phenylisothiazol-3(2H)-one
-
PCAF
0.2
5-chloro-4-methyl-2-phenylisothiazol-3(2H)-one
-
above, PCAF
0.109
5-hydroxymethyl-2-phenylisothiazol-3(2H)-one
-
PCAF
0.2
5-methyl-2-phenylisothiazol-3(2H)-one
-
above, PCAF
0.2
5-phenylureidomethyl-2-phenylisothiazol-3(2H)-one
-
above, PCAF
0.00066
acetylated histone H3 peptide
-
PCAF catalytic domain
-
0.00044
coenzyme A
-
PCAF catalytic domain
2.77
coenzyme A
-
complexed with acetyl-CoA, versus histone
5.43
coenzyme A
-
-
0.0049
garcinol
-
-
0.0039
isogarcinol
-
-
175
K+
-
-
0.0051
LTK14
-
-
0.00001
N-[2-(S-Coenzyme A)acetyl]spermidine amide
-
below
-
1.96
N6-Acetyllysine
-
complexed with acetyl-CoA, versus histone
2.5
N6-Acetyllysine
-
complexed with histone, versus acetyl-CoA
30
Na+
-
versus acetyl-CoA
34
Na+
-
versus histone
0.00002
Lys-CoA
-
inhibition of peptide acetylation
additional information
additional information
-
-
-
additional information
additional information
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.005
1,7-bis(3-bromo-4-hydroxyphenyl)-1,6-heptadiene-3,5-dione
-
-
0.033
2,6-bis(3-bromo-4-hydroxybenzylidene)cyclohexanone
-
-
0.00503
2-(3-methoxyphenyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
substrate histone H3 peptide, pH 7.5, 30C
0.0255
2-(4-(trifluoromethyl)benzyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
substrate histone H3 peptide, pH 7.5, 30C
0.13
2-(4-(trifluoromethyl)benzyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
substrate histone H3 peptide, pH 7.5, 30C
0.00072
2-(4-fluorophenyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
substrate histone H4 peptide, pH 7.5, 30C
0.00164
2-(4-fluorophenyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
substrate histone H3 peptide, pH 7.5, 30C
0.00463
2-(4-methylphenyl)isothiazolo[5,4-b]pyridin-3(2H)-one
-
substrate histone H3 peptide, pH 7.5, 30C
0.01
2-tert-butyl-5-(dodecylthio)isothiazol-3(2H)-one-1-oxide
-
above
0.01
2-tert-butyl-5-chloroisothiazol-3(2H)-one 1-oxide
-
above
0.0024
4,5-dichloro-2-ethylisothiazol-3(2H)-one
-
-
0.01
4,5-dichloro-2-ethylisothiazol-3(2H)-one-1-oxide
-
above
0.01
5-chloro-2-ethyl-4-methylisothiazol-3(2H)-one
-
above
0.01
5-chloro-2-ethyl-4-methylisothiazol-3(2H)-one-1-oxide
-
above
0.003
5-chloro-2-ethylisothiazol-3(2H)-one
-
-
0.01
5-chloro-2-ethylisothiazol-3(2H)-one-1-oxide
-
above
0.026
anacardic acid
-
inhibition of recombinant PfGCN5
0.0022 - 0.0073
CCT077791
-
IC50: 0.0022-0.0073 mM, in vivo cell proliferation inhibition, reduces acetylation of histones H3 and H4 and alpha-tubulin in cancer cell lines
0.0027 - 0.015
CCT077792
-
IC50: 0.0027-0.015 mM, in vivo cell proliferation inhibition
0.0187 - 0.0202
CCT077796
-
IC50: 0.0187-0.0202 mM, in vivo cell proliferation inhibition
0.0547
CCT079769
-
IC50: 0.0547 mM, in vivo cell proliferation inhibition
0.4
curcumin
-
value above 0.4
0.034 - 0.064
H3-CoA-20
-
IC50: 0.034-0.064 mM
-
0.012
H3-CoA-20-Tat
-
IC50: 0.012 mM, recombinant enzyme
-
0.0001
Lys-CoA
-
autoacetylation IC50: 100 nM, below
0.00031 - 0.00042
Lys-CoA
-
IC50: 310-420 nM
0.03
Lys-CoA
-
-
0.00025
Lys-CoA-Tat
-
IC50: 250 nM, recombinant enzyme, complete inhibtion of acetylation of the promyelotic leukemia zinc finger gene
0.01
methyl 3-(4,5-dichloro-1-oxido-3-oxoisothiazol-2(3H)-yl)propanoate
-
above
0.0026
methyl 3-(4,5-dichloro-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
0.0056
methyl 3-(5-chloro-1-oxido-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
0.0018
methyl 3-(5-chloro-3-oxoisothiazol-2(3H)-yl)propanoate
-
-
0.01
methyl 3-(5-chloro-4-methyl-1-oxido-3-oxoisothiazol-2(3H)-yl)propanoate
-
above
0.01
methyl 3-(5-chloro-4-methyl-3-oxoisothiazol-2(3H)-yl)propanoate
-
above
0.01
methyl 3-[4-chloro-5-(dodecylthio)-1-oxido-3-oxoisothiazol-2(3H)-yl]propanoate
-
above
0.002
Plumbagin
-
recombinant minimal HAT domain of p300
0.02
Plumbagin
-
recombinant full-length p300
0.01
methyl 3-[5-(dodecylthio)-1-oxido-3-oxoisothiazol-2(3H)-yl] propanoate
-
above
additional information
additional information
-
10, 25, 50 microM inhibit FLAG-tagged recombinant PCAF in vitro with an IC50 beyond 50 microM with purified human HeLa core histone as substrate; Hep-G2 hepatocarcinoma cells: with 5 microM plumbagin 50% reduction of histone H3 acetylation, with 25 microM 90% reduction, significant overall acetylation status of histones, prominent reduction in H3 and H4, immunofluorescence analysis (anti-acetylated histone H3 polyclonal antibodies) of HeLa cells (treated with deacetylase inhibitors to induce histone acetylation) confirm the inhibitory effect of plumbagin with 5 microM inhibitor, with 25 microM almost complete reduction in acetylation level
-
additional information
additional information
-
significant decrease of histone acetylation in plumbagin treated mouse liver in vivo 6 h after intraperitoneal injection of 25 mg plumbagin/kg body mass
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0000077
Artemia nauplii
-
enzyme form I
0.0000118
Artemia nauplii
-
enzyme forms II and III
0.00115
-
enzyme form A
0.0014
-
enzyme form B
0.0014
-
purified enzyme
0.00186
-
enzyme form B
0.00196
-
enzyme form A
0.0057
-
purified enzyme form B
0.068
-
purified enzyme
additional information
-
-
additional information
-
-
additional information
-
methods for the analysis of histone acetyltransferase activity in vitro
additional information
-
activation of p300 HAT activity and acetylation of the AR by bombesin in PC cells
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.9
-
assay at
7.4
-
assay at
7.4
-
assay at
7.5
-
-
7.5
-
assay at
7.8 - 8.8
-
enzyme form B, substrate spermidine
7.8
-
-
7.8
-
enzyme form B, substrate histone
7.8
-
assay at
7.9 - 8.6
-
enzyme form B
7.9
Q9H8E8
assay at
8 - 9.2
-
assay at
8
-
assay at; PCAF protein
8
-
assay at; tGnc5 domain
8
-
assay at
8
Q9UTM7
assay at
8.1
-
enzyme form A
8.2 - 8.5
-
enzyme form B
8.2
-
assay at
8.3
-
assay at
8.5 - 8.8
-
enzyme form A, substrate spermidine
8.7
-
enzyme form B1 and B2
8.7
-
spermidine
8.8
-
enzyme form A, substrate histine
additional information
-
-
additional information
-
pI: 7.8-8.2
additional information
-
reaction kinetic is pH-dependent
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 9.5
-
PCAF protein
6.5 - 9.5
-
less than 10% of activity maximum below pH 6.5 and above pH 9.5
6.6 - 8.4
-
about 50% of activity maximum at pH 6.6 and pH 8.4
additional information
-
pH profiles of wild-type and mutant enzymes
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 37
-
-
22 - 37
-
assay at
22
-
assay at room temperature
22
-
assay at room temperature
30
Q8WYB5
MORF protein, assay at
30
-
NuA4-like protein, assay at
30
-
PCAF protein, assay at
30
-
tGcn5 domain
30
-
assay at
30
-
assay at
30
-
assay at
37
-
about
37
-
assay at
37
-
assay at
37
-
assay at
37
-
assay at
37
-
enzyme form B
37
Q9UTM7
assay at
37
Q9H8E8
assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4 - 47
-
4-37C: activity maximum, 47C: 50% of activity maximum
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
of embryos
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
-
mushroom bodies, expression of Enok
Manually annotated by BRENDA team
-
the Qkf gene, in addition to being strongly expressed in the developing cerebral cortex and in adult neural stem cells, is also expressed in post-mitotic cells such as neurons
Manually annotated by BRENDA team
-
african green monkey kidney cell line CV-1 infected with SV40
Manually annotated by BRENDA team
-
H4 acetylation at origins by HBO1 is cell-cycle regulated, with maximal activity at the G1/S transition
Manually annotated by BRENDA team
-
cortex, CBP is expressed throughout embryogenesis, enzyme levels are decreasing postnatally
Manually annotated by BRENDA team
-
E14.1, 129/Ola cells
Manually annotated by BRENDA team
-
primary dermal
Manually annotated by BRENDA team
-
HAM1 or the HAM2 genes display an overlapping expression pattern, mainly in growing organs such as shoots and flower buds
Manually annotated by BRENDA team
Artemia nauplii
-
dormant
Manually annotated by BRENDA team
-
in the embryonic cortex, nestin-positive precursors and betaII-tubulin positive neurons from E12-E13 primary cortical precursor cell culture
Manually annotated by BRENDA team
-
colon tumor cell line
Manually annotated by BRENDA team
-
zMoz expression is restricted to the zebrafish head
Manually annotated by BRENDA team
Danio rerio zMoz
-
zMoz expression is restricted to the zebrafish head
-
Manually annotated by BRENDA team
-
p53 modulation assay
Manually annotated by BRENDA team
-
liver cancer cell line, histones are hyperacetylated in hepatocarcinomas
Manually annotated by BRENDA team
-
tissue culture cell, HTC cells
Manually annotated by BRENDA team
-
colon tumor cell line
Manually annotated by BRENDA team
-
meristem and stamen
Manually annotated by BRENDA team
-
Tip60 co-localizes with the UHRF1/DNMT1 complex
Manually annotated by BRENDA team
-
human embryonic kidney A293 cells
Manually annotated by BRENDA team
-
expressed in embryonic lens. The lens-specific chromatin domain contains both promoter localized CBP on the background of locus spread-presence of CBP and p300
Manually annotated by BRENDA team
-
fetal, expression of Moz
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
fetal
-
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
-
e.g. GFAP-positive astrocyytes and A2B5-positive oligodendrocyte precursors
Manually annotated by BRENDA team
-
the embryonic cortex, nestin-positive precursors and betaII-tubulin positive neurons from E12-E13 primary cortical precursor cell culture
Manually annotated by BRENDA team
-
the Qkf gene, in addition to being strongly expressed in the developing cerebral cortex and in adult neural stem cells, is also expressed in post-mitotic cells such as neurons
Manually annotated by BRENDA team
Q8LPU4
meristematic part of root of embryos
Manually annotated by BRENDA team
-
axe, i.e. embryo
Manually annotated by BRENDA team
Zea mays Cuzco
-
i.e. embryo
-
Manually annotated by BRENDA team
-
neuroblastoma
Manually annotated by BRENDA team
-
HAM1 or the HAM2 genes display an overlapping expression pattern, mainly in growing organs such as shoots and flower buds
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6
-
-
-
Manually annotated by BRENDA team
-
leukemia cell line
Manually annotated by BRENDA team
additional information
Q8LPU4
preformed enzyme form B is stored during embryogenesis to be available at the very early germination peroid
Manually annotated by BRENDA team
additional information
-
ubiquitous expression of MORF protein in human tissue
Manually annotated by BRENDA team
additional information
-
fairly constant levels of Hat1 protein throughout the cell cycle, soluble histone H4 Hat1-dependently acetylated on Lys12 is present in cells arrested at all cell cycle stages, G1, S, G2/M and also G0
Manually annotated by BRENDA team
additional information
-
Moz gene is expressed throughout the developing embryo and in most adult organs
Manually annotated by BRENDA team
additional information
-
organ-specific expression patterns of HAT genes, overview
Manually annotated by BRENDA team
additional information
-
semiquantitative determination of enzyme in primary cancers of different tissues, overview. Hbo1 is approximately equimolar with the number of active replication origins in normal human fibroblasts but is an order of magnitude more abundant in both MCF7 and Saos-2 established cancer cell lines. Strong Hbo1 protein expression in carcinomas of the testis, ovary, breast, stomach/esophagus, and bladder
Manually annotated by BRENDA team
additional information
-
Tip60 is part of the evolutionarily conserved NuA4 complex
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
enzyme form A
Manually annotated by BRENDA team
Artemia nauplii
-
enzyme form I
Manually annotated by BRENDA team
Q8LPU4
a minor part of enzyme form B is chromatin associated
Manually annotated by BRENDA team
-
enzyme form CI and CII
Manually annotated by BRENDA team
Zea mays Cuzco
-
enzyme form B
-
Manually annotated by BRENDA team
-
cytosolic Hat1-RbAp48 associate with histones H3 and H4, and Asf1 in vivo
Manually annotated by BRENDA team
-
catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC)
Manually annotated by BRENDA team
-
closely associated with, enzyme forms B and DB
Manually annotated by BRENDA team
Artemia nauplii
-
enzyme forms II and III
Manually annotated by BRENDA team
-
enzyme forms B and DB
Manually annotated by BRENDA team
-
enzyme forms NI and NII
Manually annotated by BRENDA team
-
macronucleus, enzyme form A
Manually annotated by BRENDA team
-
enzyme form A and B
Manually annotated by BRENDA team
-
enzyme forms I and II
Manually annotated by BRENDA team
-
enzyme form A1 and A2
Manually annotated by BRENDA team
-
HAT-B complex is mainly localized in
Manually annotated by BRENDA team
-
recruitment of Hat1p to chromatin is linked to DNA double-strand breaks, e.g. at the MAT locus, nuclear Hat1p-associated histone chaperone Hif1p is also recruited to an endonuclease HO-induced double-strand break with a similar distribution, the enzyme influences the chromatin structure at double-strand breaks, its recruitment to double-strand breaks is independent of recombinational repair, overview
Manually annotated by BRENDA team
-
HBO1 is targeted to origins through a direct interaction with the licensing regulator Cdt1
Manually annotated by BRENDA team
Q8III2
and cytoplasm
Manually annotated by BRENDA team
Mus musculus C57BL/6, Danio rerio zMoz
-
-
-
Manually annotated by BRENDA team
-
catalytic site facing the lumen of the endoplasmic reticulum/endoplasmic reticulum Goli intermediate compartment (ER/ERGIC)
Manually annotated by BRENDA team
additional information
-
recruitment of HATs and HDACs by IkappaBalpha, IKKalpha and IKKgamma
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Human immunodeficiency virus type 1 group M subtype B
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)