Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | above 10 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
S-adenosyl-L-methionine | - |
Homo sapiens | |
0.0077 | - |
native Micrococcus lysodeikticus DNA containing cytosine | - |
Homo sapiens | |
0.015 | - |
P815 DNA containing cytosine | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
135000 | - |
glycerol gradient centrifugation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | soft tissue of | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000166 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, 30% glycerol, activity decays at a rate of about 15% in 2 weeks | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + DNA | - |
Homo sapiens | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + DNA | the enzyme methylates DNA from various sources in native and heat-denatured forms. The synthetic copolymer poly(dG-dC)*poly(dG-dC) is methylated in B-conformation and in Z-conformation to about the same extent, determination of maintenance DNA methylase activity with P815 DNA and determination of de novo DNA methylase activity with 5-methylcytosine-free procaryotic DNA | Homo sapiens | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + native Micrococcus lysodeikticus DNA containing cytosine | - |
Homo sapiens | S-adenosyl-L-homocysteine + native Micrococcus lysodeikticus DNA containing 5-methylcytosine | - |
? | |
S-adenosyl-L-methionine + P815 DNA containing cytosine | - |
Homo sapiens | S-adenosyl-L-homocysteine + P815 DNA containing 5-methylcytosine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Homo sapiens |