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a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine
a 5-methyl-2'-deoxycytidine in DNA + H+ + S-adenosyl-L-homocysteine
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
DNA fragment RPS + S-adenosyl-L-methionine
DNA fragment RPS containing 5-methylcytosine + S-adenosyl-L-homocysteine
RPS is a repetitive hypermethylated DNA fragment from Petunia hybrida. CG methylation, CNG methylation, and CNN methylation. MET1 maintains CG methylation, and DRM1/2 and CMT3 act redundantly to enforce non-CG methylation, unusual cooperative activity of all three DNA methyltransferases is therefore required for maintenance of both CG and non-CG methylation in RPS. Arabidopsis thaliana does not contain any RPS homologues. Methylation at the CCmTGG site also requires DRM1/2, MET1, and, to a lesser extent, CMT3
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?
plasmid pSDTV28 + S-adenosyl-L-methionine
plasmid pSDTV28 containing 5-methylcytosine + S-adenosyl-L-homocysteine
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?
poly(dG-mdC)-poly(dG-dC) + S-adenosyl-L-methionine
?
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-
-
?
poly(dI-mdC)-poly(dI-dC) + S-adenosyl-L-methionine
?
-
-
-
?
S-adenosyl-L-methionine + (CGG*CCG)12
?
-
-
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?
S-adenosyl-L-methionine + (CGG*CCG)73
?
-
-
-
?
S-adenosyl-L-methionine + (CGG*CGG)12
?
-
-
-
?
S-adenosyl-L-methionine + (CMeGG-CCG)12
S-adenosyl-L-homocysteine + (CMeGG-CCG)12 containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + CpA
S-adenosyl-L-homocysteine + CpA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + CpG
S-adenosyl-L-homocysteine + CpG containing 5-methylcytosine
S-adenosyl-L-methionine + CpNpG
?
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-
-
-
?
S-adenosyl-L-methionine + CpNpN
?
-
-
-
-
?
S-adenosyl-L-methionine + CpT
S-adenosyl-L-homocysteine + CpT containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + dGdC
?
-
-
-
?
S-adenosyl-L-methionine + dIdC
?
-
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-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
S-adenosyl-L-methionine + hemimethylated CpG
?
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-
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-
?
S-adenosyl-L-methionine + linear pRW 3602 DNA containing cytosine
S-adenosyl-L-homocysteine + linear pRW 3602 DNA containing 5-methylcytosine
-
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-
?
S-adenosyl-L-methionine + Micrococcus luteus DNA containing cytosine
S-adenosyl-L-homocysteine + Micrococcus luteus DNA containing 5-methylcytosine
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-
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-
?
S-adenosyl-L-methionine + mononucleosomal DNA containing cytosine
S-adenosyl-L-homocysteine + mononucleosomal DNA containing 5-methylcytosine
-
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?
S-adenosyl-L-methionine + native Micrococcus lysodeikticus DNA containing cytosine
S-adenosyl-L-homocysteine + native Micrococcus lysodeikticus DNA containing 5-methylcytosine
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-
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-
?
S-adenosyl-L-methionine + P815 DNA containing cytosine
S-adenosyl-L-homocysteine + P815 DNA containing 5-methylcytosine
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-
-
-
?
S-adenosyl-L-methionine + poly (dI-dC)
S-adenosyl-L-homocysteine + poly (dI-dC) containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + poly(dG-dC)
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dG-dC)-poly(dG-dC)
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dI*dC-dI*dC) of chain length 100
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dI*dC-dI*dC) of chain length 2000
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dI*dC-dI*dC) of chain length 500
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dI*dC-dI*dC) of chain length 5000
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dI-dC)
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dI-dC)*poly(dI-dC)
?
S-adenosyl-L-methionine + poly(dI-dC)-poly(dI-dC)
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dI-dC)/poly(dI-dC)
S-adenosyl-L-homocysteine + poly(dI-dC)/poly(dI-dC) containing 5-methylcytosine
-
-
-
-
?
S-adenosyl-L-methionine + poly(dIdC:dIdC)
?
-
-
-
-
?
S-adenosyl-L-methionine + poly-(dI-dC)*poly(dI-dC)
?
-
-
-
?
S-adenosyl-L-methionine + poly-(dI-dC)/poly(dI-dC)
?
-
-
-
-
?
S-adenosyl-L-methionine + relaxed circular pRW 3602 DNA containing cytosine
S-adenosyl-L-homocysteine + relaxed circular pRW 3602 DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + supercoiled pRW 3602 DNA containing cytosine
S-adenosyl-L-homocysteine + supercoiled pRW 3602 DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + tRNAAspGUC
S-adenosyl-L-homocysteine + tRNAAspGUC containing 5-methylcytosine
-
-
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-
?
S-adenosyl-L-methionine + tRNAGlyGCC
S-adenosyl-L-homocysteine + tRNAGlyGCC containing 5-methylcytosine
-
-
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-
?
S-adenosyl-L-methionine + tRNAValAAC
S-adenosyl-L-homocysteine + tRNAValAAC containing 5-methylcytosine
-
-
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?
S-adenosyl-L-methionine + unmethylated 30-mer DNA containing cytosine
S-adenosyl-L-homocysteine + unmethylated 30-mer DNA containing 5-methylcytosine
additional information
?
-
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
the enzyme has distinct target sequences but no preferred methylation sites of promoters or other regulatory elements
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
substrate is genomic DNA of Drosophila melanogaster
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
the Z2389 DNAcytosine methyltransferase confers full protection to NotI sites by methylation of the first cytosine residue
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA methyltransferase M.EcoHK31I methylates the inner cytosine of 5'-YGGCCR-3'
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
Z2389 methylates NotI site , i.e. GCGGCCGC, and EagI site, both at the first cytosine residue
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
cytosine DNA methyltransferase M.HhaI modifies the internal cytosine within its cognate recognition site 5'-GCGC-3'. Binding of either unmethylated or hemimethylated cognate DNA to the cofactor-bound binary complex leads to dramatic changes in the 1H-15N correlation spectrum, analysis of hemimethylated and methylated, cognate and noncognate substrate binding , detailed overview
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
double-stranded DNA substrates with CpG site, overview
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
both DNMT3A and DNMT3B are involved in de novo DNA methylation. 5'-Cytosine methylation is a common epigenetic modification in eukaryotic genomes
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
DNA cytosine methylation is one of the major epigenetic gene silencing marks in the human genome facilitated by DNA methyltransferases
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
Dnmt1 methylates DNA at CG sites. Substrate binding and involved motifs in Dnmt1, overview
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
double-stranded DNA substrates with CpG site containing 8-oxo-7,8-dihydroguanine, resulting in diminished enzymatic methylation of its 5' neighboring cytosine, or 8-oxoG or O6-methylguanine in the CpG site, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
poly(dI-dC) substrate DNA. The enzyme contains the zinc-finger CXXC domain, a zinc-coordinating domain that selectively binds unmethylated DNA and is crucial for enzymatic activity
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
a hemimethylated 5MeCpG target site is generated using Sau3AI restriction enzyme
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
both DNMT3A and DNMT3B are involved in de novo DNA methylation. 5'-Cytosine methylation is a common epigenetic modification in eukaryotic genomes
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNMT1 participates in epigenetic reprogramming through its ability to distinguish different categories of methylated sequences. Genomic imprinting is a mammalian epigenetic process that distinguishes maternal and paternal alleles to ensure parent-specific, monoallelic expression of imprinted genes. Preimplantation DNMT1-dependent maintenance mechanism specifically targets DMD sequences, e.g. of IAP, alpha-actin, Snurf/Snrpn, H19, Gnas, and Gtl2 DMD, overview
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
substrate is chloroplast DNA, no activity with mitochondrial DNA. Substrate sources are etiolated leaf-rice DNA, dry embryo-rice DNA, 10-day-old shoot-rice DNA, mature leaf-rice DNA. The purified protein prefers hemi-methylated DNA substrate, substrate specificity, the enzyme is specific for d(C-G) sites, overview
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNMT1 is crucial for cell survival
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine, molecular mechanism of substrate recognition, overview
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?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
M.SssI is the only known prokaryotic C5-MTase, which recognizes the short sequence CG and thus has the same specificity as mammalian MTases
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-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
structural basis for DNA recognition and base flipping, overview
-
-
?
S-adenosyl-L-methionine + CpG
S-adenosyl-L-homocysteine + CpG containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + CpG
S-adenosyl-L-homocysteine + CpG containing 5-methylcytosine
-
-
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?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Bacillus subtilis phage PHI3T
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?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Bacillus subtilis phage PHI3T
-
enzyme methylates the cytosine of the DNA target sequence TCGA
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?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Bacillus subtilis phage rho11S
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?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Bacillus subtilis phage rho11S
-
enzyme methylates the cytosine of the DNA target sequence TCGA
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?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
about 60% of the total methylation occurs in the 5'd(C-G)3' doublet. Single-stranded and hemi-methylated DNAs are methylated at an elevated rate
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?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
cellular organism
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?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
the enzyme is unable to make de novo methylation in double stranded DNA, methylation occurs at maximal rate on hemimethylated ds DNA
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
nonselective cytosine methylation activity. Chloroplast DNA methylation by DMT1 is one of the factors influencing maternal inheritance of chloroplast genes
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?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
nonselective cytosine methylation activity
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-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
ir
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
transfer of methyl groups to heterologous DNA
-
ir
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
site-specificity
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
methylation targets of M.BssHII: ACGCGT/CCGCGG MluI/SacII, PuGCGCPy HaeII, PuCCGGPy Cfr10I and GCGCGC BssHII
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
methylation targets of M.BssHII: ACGCGT/CCGCGG MluI/SacII, PuGCGCPy HaeII, PuCCGGPy Cfr10I and GCGCGC BssHII
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
the enzyme is able to methylate homologues HeLa DNA, altough to a lesser extent than heterologous DNA. The enzyme can methylate single-stranded DNA three times higher than that of the corresponding double-stranded DNA, the 5' neighbor can be either G or C while the 3' neighbor is always G, enzyme can methylate poly(dG,dC)
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
methylation of double-stranded Micrococcus luteus DNA. The enzyme transfers methyl groups to both double-stranded and single-stranded natural DNAs. The methyl-accepting activity of the DNA is correlated to their GC content, homologous DNAs are poor substrates. Very low rate of methylation with poly[(dA-dC)*(dG-dT)], highest methyl-accepting ability with poly[(dG-dC)*(dG-dC)]
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
the enzyme methylates DNA from various sources in native and heat-denatured forms. The synthetic copolymer poly(dG-dC)*poly(dG-dC) is methylated in B-conformation and in Z-conformation to about the same extent, determination of maintenance DNA methylase activity with P815 DNA and determination of de novo DNA methylase activity with 5-methylcytosine-free procaryotic DNA
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
methylation of a series of snapback oligodeoxynucleotides of differing stem length, each containing a centrally located CG dinucleotide recognition site. The enzyme requires a minimum of 22 base pairs in the stem for maximum activity. Extrahelical cytosines in slipped duplexes that are 30 base pairs in length act as effective methyl acceptors and are more rapidly methylated than cytosines that are Watson-Crick paired. Duplexes containing hairpins of CCG repeats in cruciform structures in which the enzyme recognition sequence is disrupted by a C-C mispair are also more rapidly methylated than the control Watson-Crick-paired duplexes
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
poly(dI-dC)*poly(dI-dC) and unmethylated and hemimethylated 36-mer and 75-mer oligonucleotides. The preference of the enzyme for hemimethylated, over unmethylated DNA is 7-21fold. Poly(dI-dC)poly(dI-dC)
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
DNA topology strongly influences the reaction rate, which increases with increasing negative superhelical tension
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
DNMT1 interacts with hSNF2H chromatin remodeling enzyme and binds mononucleosomes with higher affinity in the presence of hSNF2H
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
DNMT3B interacts with several components of the condensin complex (hCAP-C,hCAP-E and hCAP-G) and KIF4A. Condensin mediates genome-wide chromosome condensation at the onset of mitosis and is critical for proper segregation of sister chromatids. KIF4A is proposed to be a motor protein carrying DNA as cargo. DNMT3B also interacts with histone deacetylase 1 (HDAC1), the co-repressor SIN3A and the ATP-dependent chromatin remodeling enzyme hSNF2H. DNMT3B co-localizes with condensin and KIF4A on condensed chromosomes throughout mitosis, direct link between the machineries regulating DNA methylation and mitotic chromosome condensation. DNMT3B may have a previously unrecognized function during the mitotic phase of the cell cycle
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
hypermethylation of CpG islands in the promoter regions is an important mechanism to silence the expression of many important genes in cancer
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
control of Dnmt1, the predominant maintainance methyltransferase, is significantly suppressed in aging cells and contributes to the reduced genomic methylation of these cells. The paradoxical sporadic gene hypermethylation in aging cells appears to be related to transcriptional up-regulation of the Dnmt3b gene. Changes in transcriptional control of the Dnmts are the likely cause for the known alterations in DNA methylation in aging cells and in cells undergoing tumorigenesis
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
free DNA or assembled into a mononucleosome. The catalytic efficiency decreases on the mononucleosome 17fold
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
free DNA or assembled into a mononucleosome. The catalytic efficiency decreases on the mononucleosome 8fold
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
methylation is observed at CG sites in a loose ttnCGga(g/a) consensus sequence
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
poly-(dI-dC)/poly(dI-dC), wild-type enzyme and N-terminal deletion mutants lacking 121, 501, 540, or 580 amino acids from the N-terminus methylate CG sequences, prefer hemimethylated to unmethylated DNA
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
the enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine onto the 5'-position of the cytosine ring of the DNA
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
the enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine onto the 5 position of the cytosine ring of the DNA
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
the enzyme recognizes the sequence 5'-CCGG-3' and methylates the outer cytosine residue
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
both native and denatured DNA are methylated, with calf thymus DNA the double stranded form is the better substrate but the enzyme preferentially methylates single stranded E. coli DNA even in the native preparation. Homologous ascites DNA can be methylated, but only to about 5% of the level of the best substrate, undermethylated mouse L929 cell DNA. DNA isolated from growing cells or tissues is a better substrate than DNA from normal liver or pancreas or from stationary cells
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
methylation of double-stranded Micrococcus luteus DNA. The enzyme transfers methyl groups to both double-stranded and single-stranded natural DNAs. The methyl-accepting activity of the DNA is correlated to their GC content, homologous DNAs are poor substrates. Very low rate of methylation with poly[(dA-dC)*(dG-dT)], highest methyl-accepting ability with poly[(dG-dC)*(dG-dC)]
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
the purified enzyme has at least a 30fold higher catalytic efficiency with hemimethylated double-stranded oligodeoxyribonucleotide substrates than unmethylated substrates and is most active with small oligodeoxyribonucleotide substrates
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
poly(dIdC:dIdC) is efficiently methylated
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
higher methylating activity with chemically synthesized hemimethylated oligodeoxynucleotide than with poly(dI*dC)*poly(dI*dC)
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
poly(dI*dC-dI*dC) of chain length 100, 500, 2000 or 5000 base pairs
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
specificity is confined to the sequence 5'-CpG-3'. Any sequence-specific de novo methylation mediated by Dnmt1 is either under the control of regulatory factors that interact
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
isoenzymes Dnmt3a, Dnmt3b1 and Dnmt3b2 show similar activity towards poly(dG-dC)-poly(dG-dC)
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Dnmt1 plays an essential role in the faithful and efficient maintenance of methylation patterns in the mammalian genome
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
major enzyme in maintenance of the pattern of DNA methylation after DNA replication
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
the enzyme is a de novo-type DNA methyltransferase
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
methylates DNA processively with high preference for hemimethylated target sites. Dnmt1 methylates a hemimethylated 958-mer substrate in a highly processive reaction. Fully methylated and unmethylated CG sites do not inhibit processive methylation of the DNA. Unmethylated sites embedded in a hemimethylated context are modified at an approximately 24fold reduced rate. Completely unmodified DNA is methylated even more slowly. Dnmt1 is not able to methylate hemimethylated CG sites on different strands of the DNA in a processive manner, indicating that Dnmt1 keeps its orientation with respect to the DNA while methylating the CG sites on one strand of the DNA
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
P-19 cells possess catalytically active Dnmt1, Dnmt2, Dnmt3a and Dnmt3b. Dnmt1 and Dnmt3b have the greatest amount of methylation activity directed at the endogenous genome
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
poly(dGdC)-poly(dGdC) or poly(dIdC)-poly(dIdC)
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
preference for single stranded DNA substrates is increased up to 50fold by the presence of a proximal 5-methyl cytosine. This modulation is distance-dependent and is due to an enhanced binding affinity and minor changes in catalytic efficiency. No modulation of activity is observed with double stranded DNA
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
unmethylated DNA substrates: poly(dIdC)-poly(dIdC), poly(dGdC)-poly(dGdC) or a 520-bp DNA fragment from the p2HhaBsp. Full-length Dnmt1 methylates hemimethylated DNA with high processivity and a fidelity of over 95%, but unmethylated DNA with much less processivity. Dnmt1-(2911620), the truncated form of Dnmt1, shows identical properties to full-length Dnmt1 indicating that the N-terminal 290-amino acid residue region of Dnmt1 is not required for preferential activity toward hemimethylated sites or for processivity of the enzyme. Dnmt1 methylates hemimethylated CpG sites on one strand of double-stranded DNA during a single processive run
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
NtDRM1 is a de novo cytosine methyltransferase which actively excludes CpG substrate
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
poly(dI-dC) is a better substrate than poly(dG-dC), methylation activity toward unmethylated synthetic as well as native DNA. Activity with hemimethylated DNA is lower than that for unmethylated substrates. Methylation of about 70% of the cytosines in methylatable CpNpN and CpNpG sequences but only 10% in CpG. The enzyme non-selectively methylates any cytosines except in CpG, regardless of the adjacent nucleotide at both 5' and 3' ends
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
native plant DNA or hemimethylated poly(dI-MedC)*poly(dI-dC)
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
native and heat-denatured DNA from ethionine-treated synchronized Novikoff cells. The native DNA sample is about 3.5 fold better as a methyl acceptor than the heat-denatured sample
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
the enzyme is able to methylate single stranded and double stranded DNA. Previously unmethylated double-stranded DNA from prokaryotes, Micrococcus luteus, as well as from eukaryotes, Ascaris suis, can serve as substrates. The synthetic copolymers (dG-dC)n*(dC-dG)n and (dG,dC)n are also methylated. SV40 DNA is almost not methylated. PM2 DNA is a good substrate even in the supercoiled form. The enzyme methylates 1 in 17 bases in heterologous Micrococcus luteus DNA, but only 1 in 590 in homologous rat liver DNA
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
methylation of cytosine residues in double-stranded DNAs isolated from wheat, maize, calf thymus or bacteria. Single-stranded DNAs from all sources are methylated to only about one-fourth to one-third the degree of double-stranded substrate DNA. Synthetic polydeoxyribonucleotides of alternating base sequence, poly[d(G-C)]*poly[d(G-C)], poly[d(I-C)]*poly[d(I-C)], poly[d(A-C)]*poly[d(G-T)], are methylated to low but clearly measurable extent. Preference for endogenous, double-stranded DNA
-
?
S-adenosyl-L-methionine + poly(dI-dC)*poly(dI-dC)
?
-
-
-
-
?
S-adenosyl-L-methionine + poly(dI-dC)*poly(dI-dC)
?
-
-
-
?
S-adenosyl-L-methionine + unmethylated 30-mer DNA containing cytosine
S-adenosyl-L-homocysteine + unmethylated 30-mer DNA containing 5-methylcytosine
-
-
-
?
S-adenosyl-L-methionine + unmethylated 30-mer DNA containing cytosine
S-adenosyl-L-homocysteine + unmethylated 30-mer DNA containing 5-methylcytosine
-
-
-
?
additional information
?
-
DNA fragment RPS belongs to a group of middle repetitive, dispersed and hypermethylated homologues. Repetitiveness, however, is not a prerequisite for hypermethylation, as RPS transgenes are efficient methylation targets in Arabidopsis, which lacks any significant RPS homology
-
-
?
additional information
?
-
-
DNA fragment RPS belongs to a group of middle repetitive, dispersed and hypermethylated homologues. Repetitiveness, however, is not a prerequisite for hypermethylation, as RPS transgenes are efficient methylation targets in Arabidopsis, which lacks any significant RPS homology
-
-
?
additional information
?
-
-
tRNA binding studies show that EhMeth interacts with a RNA substrate only containing 17 nucleotides of the tRNA anticodon stem-loop (including the substrate cytosine C38), but requires a full-length tRNA for stable complex formation
-
-
?
additional information
?
-
-
using a yeast two-hybrid screen enolase (EC 4.2.1.11) is identified as a Dnmt2-binding protein, acting as a Dnmt2 inhibitor
-
-
?
additional information
?
-
-
site-based methylation is dependent on the relative position of the zinc finger binding sites and the target methylation site, methylation specificity for the target site also depends on zinc finger binding
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
-
reorganization of an essential catalytic loop, residues 80-100, is regulated by sequence-specific protein-DNA interactions, DNA-dependent positioning of the catalytic loop
-
-
?
additional information
?
-
-
the enzyme is capable of protecting plasmid DNA in vivo against action of the cognate restriction endonuclease
-
-
?
additional information
?
-
the entire reaction mechanism of DNMT is divided into six steps, which are scanning, recognition, flipping, loop locking, methylation, and releasing.
-
-
-
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
role of the enzyme in maintaining the methylation patterns throughout development, the enzyme may be involved in the etiology of fragile X, a syndrome characterized by de novo methylation of a greatly expanded CGG-CCG triplet repeat sequence
-
-
?
additional information
?
-
-
role of the enzyme in maintaining the methylation patterns throughout development, the enzyme may be involved in the etiology of fragile X, a syndrome characterized by de novo methylation of a greatly expanded CGG-CCG triplet repeat sequence
-
-
?
additional information
?
-
-
upon activation of ER-target gene expression, CpG dinucleotides of promoters undergo cyclical demethylation and remethylation with a cycle time of roughly 2 h, cyclical recruitment of DNMT3A and DNMT3B DNA methyltransferases to the promoter regions of estrogen receptoralpha target genes. In cancer cells, DNMT3A and DNMT3B might posses deaminase activity and be involved in a dynamic demethylation-methylation pathway that operates during gene transcription, overview
-
-
?
additional information
?
-
-
homology docking and molecular dynamics, overview
-
-
?
additional information
?
-
no activity with single-stranded DNA with one Cm5 site, double-stranded fully methylated DNA, and poly(dA)-poly(T), pol(G), poly(dC)-poly(dG) or poly(dA-dT)
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
-
intrinsic sequence-specificity of Dnmt1 on linear duplex DNA is unlikely to be important in the establishment of genomic methylation patterns
-
-
?
additional information
?
-
-
the enzyme is essential for viable mammalian development and has a central function in the determination and maintenance of epigenetic methalation pattern
-
-
?
additional information
?
-
-
enzymatic DNA methylation of carbon 5 of cytosines is an epigenetic modification that plays a role in regulating gene expression, differentiation, and tumorigenesis. DNA (cytosine-C5)-methyltransferase-1 is the enzyme responsible for maintaining established methylation patterns during replication in mammalian cells
-
-
?
additional information
?
-
no activity with CpT and CpC
-
-
?
additional information
?
-
no activity with CpT and CpC
-
-
?
additional information
?
-
-
no activity with CpT and CpC
-
-
?
additional information
?
-
scarcely methylates CpC
-
-
?
additional information
?
-
scarcely methylates CpC
-
-
?
additional information
?
-
-
scarcely methylates CpC
-
-
?
additional information
?
-
interactions between Dnmt3b and both Tdg and Mbd4, i.e. G/T mismatch-specific thymine-DNA glycosylase and methyl-CpG binding domain protein 4, two thymine glycosylases involved in reduction of the impact of 5mC deamination, that can both excise uracil or thymine at U-G and T-G mismatches to initiate base excision repair, overview. Interaction with Tdg via two separate Dnmt3b domains, but MTase motif I of the catalytic domain of Dnmt3b is sufficient for interaction with Tdg and Mbd4
-
-
?
additional information
?
-
-
upon activation of ER? target gene expression, CpG dinucleotides of promoters undergo cyclical demethylation and remethylation with a cycle time of roughly 2 h, cyclical recruitment of DNMT3A and DNMT3B DNA methyltransferases to the promoter regions of estrogen receptoralpha target genes. In cancer cells, DNMT3A and DNMT3B might posses deaminase activity and be involved in a dynamic demethylation-methylation pathway that operates during gene transcription, overview
-
-
?
additional information
?
-
MarII is a GCGC site recognizing DNA methyltransferase analogous to M.HhaI, not M.MarI, overview
-
-
?
additional information
?
-
-
MarII is a GCGC site recognizing DNA methyltransferase analogous to M.HhaI, not M.MarI, overview
-
-
?
additional information
?
-
MarII is a GCGC site recognizing DNA methyltransferase analogous to M.HhaI, not M.MarI, overview
-
-
?
additional information
?
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
Q10C15
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
Q8H854
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
methylation of genomic DNA is involved in the basic mechanism of gene inactivation, chromatin organization, X-chromosome inactivation, and genomic imprinting
-
-
?
additional information
?
-
-
the enzyme catalyzes the methylation of DNA during replication, in NGF-induced PC12 cell. The enzyme activity is sharply reduced 4 days after induction of differentiation
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
enzyme can additionally deaminate cytosine to uracil in a slow reaction in the absence of SAM, and the rate of this reaction can be increased by the SAM analogue 5'-amino-5'-deoxyadenosine. SssI-catalyzed deamination of C5-methylcytosine is not detected
-
-
?
additional information
?
-
enzyme can additionally deaminate cytosine to uracil in a slow reaction in the absence of SAM, and the rate of this reaction can be increased by the SAM analogue 5'-amino-5'-deoxyadenosine. SssI-catalyzed deamination of C5-methylcytosine is not detected
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
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DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
additional information
?
-
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
the enzyme has distinct target sequences but no preferred methylation sites of promoters or other regulatory elements
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
the Z2389 DNAcytosine methyltransferase confers full protection to NotI sites by methylation of the first cytosine residue
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
both DNMT3A and DNMT3B are involved in de novo DNA methylation. 5'-Cytosine methylation is a common epigenetic modification in eukaryotic genomes
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
DNA cytosine methylation is one of the major epigenetic gene silencing marks in the human genome facilitated by DNA methyltransferases
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
both DNMT3A and DNMT3B are involved in de novo DNA methylation. 5'-Cytosine methylation is a common epigenetic modification in eukaryotic genomes
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNMT1 participates in epigenetic reprogramming through its ability to distinguish different categories of methylated sequences. Genomic imprinting is a mammalian epigenetic process that distinguishes maternal and paternal alleles to ensure parent-specific, monoallelic expression of imprinted genes. Preimplantation DNMT1-dependent maintenance mechanism specifically targets DMD sequences, e.g. of IAP, alpha-actin, Snurf/Snrpn, H19, Gnas, and Gtl2 DMD, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNA GC content, CpG frequency and methylation status, overview
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
DNMT1 is crucial for cell survival
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
SinI DNA methyltransferase, a component of the SinI restriction-modification system, recognizes the sequence GG(A/T)CC and methylates the inner cytosine to produce 5-methylcytosine
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
-
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
-
M.SssI is the only known prokaryotic C5-MTase, which recognizes the short sequence CG and thus has the same specificity as mammalian MTases
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
nonselective cytosine methylation activity. Chloroplast DNA methylation by DMT1 is one of the factors influencing maternal inheritance of chloroplast genes
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
DNMT1 interacts with hSNF2H chromatin remodeling enzyme and binds mononucleosomes with higher affinity in the presence of hSNF2H
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
DNMT3B interacts with several components of the condensin complex (hCAP-C,hCAP-E and hCAP-G) and KIF4A. Condensin mediates genome-wide chromosome condensation at the onset of mitosis and is critical for proper segregation of sister chromatids. KIF4A is proposed to be a motor protein carrying DNA as cargo. DNMT3B also interacts with histone deacetylase 1 (HDAC1), the co-repressor SIN3A and the ATP-dependent chromatin remodeling enzyme hSNF2H. DNMT3B co-localizes with condensin and KIF4A on condensed chromosomes throughout mitosis, direct link between the machineries regulating DNA methylation and mitotic chromosome condensation. DNMT3B may have a previously unrecognized function during the mitotic phase of the cell cycle
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
hypermethylation of CpG islands in the promoter regions is an important mechanism to silence the expression of many important genes in cancer
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
the enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine onto the 5'-position of the cytosine ring of the DNA
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
the enzyme catalyzes the transfer of a methyl group from S-adenosyl-L-methionine onto the 5 position of the cytosine ring of the DNA
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Dnmt1 plays an essential role in the faithful and efficient maintenance of methylation patterns in the mammalian genome
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
major enzyme in maintenance of the pattern of DNA methylation after DNA replication
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
the enzyme is a de novo-type DNA methyltransferase
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
NtDRM1 is a de novo cytosine methyltransferase which actively excludes CpG substrate
-
-
?
additional information
?
-
DNA fragment RPS belongs to a group of middle repetitive, dispersed and hypermethylated homologues. Repetitiveness, however, is not a prerequisite for hypermethylation, as RPS transgenes are efficient methylation targets in Arabidopsis, which lacks any significant RPS homology
-
-
?
additional information
?
-
-
DNA fragment RPS belongs to a group of middle repetitive, dispersed and hypermethylated homologues. Repetitiveness, however, is not a prerequisite for hypermethylation, as RPS transgenes are efficient methylation targets in Arabidopsis, which lacks any significant RPS homology
-
-
?
additional information
?
-
-
tRNA binding studies show that EhMeth interacts with a RNA substrate only containing 17 nucleotides of the tRNA anticodon stem-loop (including the substrate cytosine C38), but requires a full-length tRNA for stable complex formation
-
-
?
additional information
?
-
-
using a yeast two-hybrid screen enolase (EC 4.2.1.11) is identified as a Dnmt2-binding protein, acting as a Dnmt2 inhibitor
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
-
the enzyme is capable of protecting plasmid DNA in vivo against action of the cognate restriction endonuclease
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
role of the enzyme in maintaining the methylation patterns throughout development, the enzyme may be involved in the etiology of fragile X, a syndrome characterized by de novo methylation of a greatly expanded CGG-CCG triplet repeat sequence
-
-
?
additional information
?
-
-
role of the enzyme in maintaining the methylation patterns throughout development, the enzyme may be involved in the etiology of fragile X, a syndrome characterized by de novo methylation of a greatly expanded CGG-CCG triplet repeat sequence
-
-
?
additional information
?
-
-
upon activation of ER-target gene expression, CpG dinucleotides of promoters undergo cyclical demethylation and remethylation with a cycle time of roughly 2 h, cyclical recruitment of DNMT3A and DNMT3B DNA methyltransferases to the promoter regions of estrogen receptoralpha target genes. In cancer cells, DNMT3A and DNMT3B might posses deaminase activity and be involved in a dynamic demethylation-methylation pathway that operates during gene transcription, overview
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
-
intrinsic sequence-specificity of Dnmt1 on linear duplex DNA is unlikely to be important in the establishment of genomic methylation patterns
-
-
?
additional information
?
-
-
the enzyme is essential for viable mammalian development and has a central function in the determination and maintenance of epigenetic methalation pattern
-
-
?
additional information
?
-
-
enzymatic DNA methylation of carbon 5 of cytosines is an epigenetic modification that plays a role in regulating gene expression, differentiation, and tumorigenesis. DNA (cytosine-C5)-methyltransferase-1 is the enzyme responsible for maintaining established methylation patterns during replication in mammalian cells
-
-
?
additional information
?
-
interactions between Dnmt3b and both Tdg and Mbd4, i.e. G/T mismatch-specific thymine-DNA glycosylase and methyl-CpG binding domain protein 4, two thymine glycosylases involved in reduction of the impact of 5mC deamination, that can both excise uracil or thymine at U-G and T-G mismatches to initiate base excision repair, overview. Interaction with Tdg via two separate Dnmt3b domains, but MTase motif I of the catalytic domain of Dnmt3b is sufficient for interaction with Tdg and Mbd4
-
-
?
additional information
?
-
-
upon activation of ER? target gene expression, CpG dinucleotides of promoters undergo cyclical demethylation and remethylation with a cycle time of roughly 2 h, cyclical recruitment of DNMT3A and DNMT3B DNA methyltransferases to the promoter regions of estrogen receptoralpha target genes. In cancer cells, DNMT3A and DNMT3B might posses deaminase activity and be involved in a dynamic demethylation-methylation pathway that operates during gene transcription, overview
-
-
?
additional information
?
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
Q10C15
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
Q8H854
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
-
MTase performs two basic kinds of methylation activities: maintenance methylation, i.e. addition of methyl groups to cytosines of a hemimethylated DNA after DNA replication, and de novo methylation, i.e. methylation of cytosines in nonmethylated DNA
-
-
?
additional information
?
-
methylation of genomic DNA is involved in the basic mechanism of gene inactivation, chromatin organization, X-chromosome inactivation, and genomic imprinting
-
-
?
additional information
?
-
-
the enzyme catalyzes the methylation of DNA during replication, in NGF-induced PC12 cell. The enzyme activity is sharply reduced 4 days after induction of differentiation
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
additional information
?
-
-
enzyme induces DNA bending, a mechanism to establish specific interface between proteins and DNA. Enzymes recognizing a cytosine 3' to the target cytosine tend to induce greater bends than enzymes with guanine in this position
-
-
?
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(-)-epigallocatechin-3-gallate
-
competitive, the inhibitor can form hydrogen bonds with Pro1223, Glu1265, Cys1225, Ser1229 and Arg1309. Hypermethylation of CpG islands in the promoter regions is an important mechanism to silence the expression of many important genes in cancer. (-)-Epigallocatechin-3-gallate can inhibit DNMT activity and reactivate methylation-silenced genes in cancer cells
(N4-fluoroacetyl-5-azacytidine)
efficient inhibitor of DNA methylation in human tumor cell lines
2'-dC
-
binding modelling, overview
2'-deoxy-5-aza-cytidine
-
binding modelling, overview
2-(1H)-pyrimidinone riboside
-
i.e. zebularine, is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds. The ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-chloro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-fluoro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methyl-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
2-amino-4-[([(2S,3S,4R,5R)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-3,4-dihydroxytetrahydrofuran-2-yl]methyl)sulfanyl]butanoic acid
-
2-pyrimidinone
forms a part of inhibitor 1,2-dihydropyrimidin-2-one-5-methylene-(methylsulfonium)-adenosyl, bindng structure and mechanism, overview
5'-(3-aminopropylthio)-5'-deoxyadenosine
5'-(3-carboxypropylthio)-5'-deoxyadenosine
5-Azacytosine
-
mechanism-based inhibitor
asCEBPA-1 RNA
competitive with respect to substrate (CMeGG-CCG)12
-
asCEBPA-2 RNA
mixed inhibition
-
asCEPA-1HPE RNA
competitive with respect to substrate (CMeGG-CCG)12
-
asCEPA-2HPE RNA
competitive with respect to substrate (CMeGG-CCG)12
-
decitabine
-
binding modelling, overview
Mg2+
1 mM, slight inhibition, Dnmt3a; 1 mM, slight inhibition, Dnmt3b
miR-127-3p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-145-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-146a-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-155-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12; micro-RNA. Exogenous miR-155-5p in cells inhibits DNMT1 and induces aberrant DNA methylation of the genome
-
miR-16-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-17-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-19b-3p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-20a-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-21-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-373-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-9-3p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-9-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-92-1-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-92a-1-5p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
miR-92a-3p
micro-RNA, competitive with respect to substrate (CMeGG-CCG)12
-
Mn2+
strong inhibition, Dnmt3a; strong inhibition, Dnmt3b
native DNA
-
non-competitive inhibitor against S-adenosyl-L-methionine
-
Ni2+
strong inhibition, Dnmt3a; strong inhibition, Dnmt3b
Nicotine
-
decreases glutamic acid decarboxylase 67 promoter methylation by DMT1 in GABAergic interneurons
NSC 106084
-
inhibition of Dnmt1
NSC 137546
-
inhibition of Dnmt1
NSC 138419
-
inhibition of Dnmt1
NSC 14778
-
inhibition of Dnmt1; inhibition of Dnmt3b
NSC 158324
-
inhibition of Dnmt1
NSC 319745
-
inhibition of Dnmt1
NSC 348926
-
slight inhibition of Dnmt1
NSC 408488
-
inhibition of Dnmt1
NSC 54162
-
inhibition of Dnmt1
NSC 56071
-
inhibition of Dnmt1
NSC 57893
-
inhibition of Dnmt1
NSC 622444
-
inhibition of Dnmt1
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone
-
oligodeoxyribonucleotides containing 2-aminopurine
-
-
-
oligodeoxyribonucleotides containing 5-azacytidine
-
5-azacytosine oligodeoxyribonucleotides form complexes with C5 DNA methyltransferases that are irreversible when the 5-azacytosine ring is intact, overview
-
poly[d(G-5-azacytidine)]
-
-
-
procainamide
-
binding modelling, overview
procaine
-
binding modelling, overview
putrescine
-
1 mM, 42% inhibition
retinoblastoma gene product
-
a negative regulator of DNA methylation, binds to the allosteric site of hDNMT1 and inhibits methylation, it may regulate methylation spreading
-
RG108
-
slight inhibition of Dnmt1
S-(1-deazaadenosyl)-L-homocysteine
-
S-(2'-deoxy-b -D-ribofuranosyladenosin-5'-yl)-L-homocysteine
-
S-(2'-deoxy-beta-D-ribofuranosyladenosin-5'-yl)-L-homocysteine
-
S-(3-deazaadenosyl)-L-homocysteine
-
S-(8-azaadenosyl)-L-homocysteine
-
S-(N-(2-biphenyl-4-ylethyl)-2-chloroadenosyl)-L-homocysteine
-
S-(N-(2-biphenyl-4-ylethyl)adenosyl)-L-homocysteine
-
S-(N-(3,5-dimethoxybenzyl)adenosyl)-L-homocysteine
-
S-(N-(pyridin-4-ylmethyl)adenosyl)-L-homocysteine
-
S-(N-benzyladenosyl)-L-homocysteine
-
S-(N-phenyladenosyl)-L-homocysteine
-
S-(N-phenylethyladenosyl)-L-homocysteine
-
S-(N-phenylpropyladenosyl)-L-homocysteine
-
S-(N6 -benzoyladenosin-5'-yl)-L-homocysteine
-
S-(N6-benzoyladenosin-5'-yl)-L-homocysteine
-
S-adenosin-5'-yl-L-homocysteine methylamide
S-adenosyl-L-homocysteine
S-cytid-5'-yl-L-homocysteine
S-inosin-5'-yl-L-homocysteine
S-nebularinehomocysteine
-
S-Tubercidinylhomocysteine
-
[1,2-dihydropyrimidin-2-one]-5-methylene-(methylsulfonium)-adenosyl
with 2-pyrimidinone ring
5'-(3-aminopropylthio)-5'-deoxyadenosine
-
5'-(3-aminopropylthio)-5'-deoxyadenosine
-
5'-(3-carboxypropylthio)-5'-deoxyadenosine
-
5'-(3-carboxypropylthio)-5'-deoxyadenosine
-
5-aza-2'-deoxycytidine
-
-
5-aza-2'-deoxycytidine
-
-
5-azacytidine
-
5-azacytidine
-
zebularine is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds
5-azacytidine
-
binding modelling, overview
5-fluorocytosine
-
mechanism-based inhibitor
6-thioguanine
-
incorporation of 6-thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivo. Presence of 6-thioguanine at the unmethylated CpG site abolished almost completely the methylation of its 5' adjacent cytosine by HpaII
6-thioguanine
-
incorporation of 6-thioguanine perturbs cytosine methylation at the CpG dinucleotide site by DNA methyltransferases in vitro and acts as a DNA demethylating agent in vivo. Presence of 6-thioguanine at the unmethylated CpG site abolished almost completely the methylation of its 5' adjacent cytosine by DNMT1
hydralazine
-
inhibition of Dnmt1
hydralazine
-
binding modelling, overview
KCl
-
above 100 mM
KCl
-
above 0.1 M, inhibition may be simply due to dissociation of the DNA-enzyme complex
NaCl
-
incubation of native calf thymus DNA in presence of 40 mM NaCl results in 50% inhibition, more than 90% inhibition at 200 mM. With denatured calf thymus DNA, low concentrations of NaCl, up to 90 mM stimulate, 50% inhibition at 175 mM
NaCl
Dnmt3a, inhibits activity around physiological ionic strength; Dnmt3b, inhibits activity around physiological ionic strength
NaCl
-
above 0.1 M, inhibition may be simply due to dissociation of the DNA-enzyme complex
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone
-
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone at the enzymatic target site are competitive inhibitors of both prokaryotic and mammalian DNA C5 methyltransferases, overview
-
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone
-
-
-
S-adenosin-5'-yl-L-homocysteine methylamide
-
S-adenosin-5'-yl-L-homocysteine methylamide
-
S-adenosyl-L-ethionine
-
non-competitive inhibitor against DNA
S-adenosyl-L-ethionine
-
IC50: 0.05 mM
S-adenosyl-L-homocysteine
-
-
S-adenosyl-L-homocysteine
-
S-adenosyl-L-homocysteine
-
S-adenosyl-L-homocysteine
-
S-adenosyl-L-homocysteine
-
product inhibition of Dnmt1; product inhibition of Dnmt3b
S-adenosyl-L-homocysteine
-
potent competitive inhibitor
S-adenosyl-L-homocysteine
-
half-inhibition at 0.02 mM
S-cytid-5'-yl-L-homocysteine
-
S-cytid-5'-yl-L-homocysteine
-
S-inosin-5'-yl-L-homocysteine
-
S-inosin-5'-yl-L-homocysteine
-
spermidine
-
1 mM, 59% inhibition
spermidine
-
at millimolar concentrations
spermine
-
1 mM, 97% inhibition
spermine
-
at millimolar concentrations
additional information
-
methylation specificity is affected by gene copy number and induction levels
-
additional information
-
inhibitor binding kinetics
-
additional information
synthesis of 2-amino-3-cyano-4-halopyridine-C-nucleosides (dXPCN) and oligodeoxyribonucleotides containing dXPCN, as inhibitors of DNMT. Oligodeoxyribonucleotides have a cyano group as an electron-withdrawing group at the C3 position of the pyridine ring. Oligodeoxyribonucleotides containing dXPCN effectively form a complex with DNMTs and exhibit cell antiproliferation activity against human cancer cells
-
additional information
not inhibited by 0.045 mM 2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
additional information
not inhibited by 0.045 mM 2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
additional information
-
not inhibited by 0.045 mM 2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
additional information
-
no inhibition of Dnmt3b by NSC 56071, NSC 348926, NSC 106084, NSC 622444, NSC 408488, NSC 137546, NSC 345763, NSC 54162, NSC 154957, NSC 158324, NSC 57893, and NSC 138419; virtual inhibitor screeening, molecular modeling with Dnmt1, overview. No inhibition of Dnmt1 by NSC 345763, NSC 154957, and NSC 158324
-
additional information
-
inhibitor screening by molecular docking and molecular dynamics simulations
-
additional information
-
stimulating proteins from murine P815 mastocytoma cells stimulate both de novo and maintenance activity of DNA methyltransferase about 3fold. They enhance the methylation of any natural DNA and of poly[(dI-dC)*(dI-dC)] but inhibit methylation of poly[(dG-dC)*(dG-dC)]
-
additional information
Dnmt3a is fully active at physiological potassium concentration of K+
-
additional information
Dnmt3a is fully active at physiological potassium concentration of K+
-
additional information
-
Dnmt3a is fully active at physiological potassium concentration of K+
-
additional information
inhibitory effects by methyl donor analogs, base analogs, cations, and cationic amines on rice DNA MTase; inhibitory effects by methyl donor analogs, base analogs, cations, and cationic amines on rice DNA MTase
-
additional information
inhibitory effects by methyl donor analogs, base analogs, cations, and cationic amines on rice DNA MTase; inhibitory effects by methyl donor analogs, base analogs, cations, and cationic amines on rice DNA MTase
-
additional information
-
inhibitory effects by methyl donor analogs, base analogs, cations, and cationic amines on rice DNA MTase; inhibitory effects by methyl donor analogs, base analogs, cations, and cationic amines on rice DNA MTase
-
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0.045
1-deaza-S-adenosyl-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0029 - 0.0572
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-chloro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.0025 - 0.0068
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-fluoro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.021 - 0.256
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.075
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.001 - 0.006
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methyl-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.216 - 0.5
2-amino-4-[([(2S,3S,4R,5R)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-3,4-dihydroxytetrahydrofuran-2-yl]methyl)sulfanyl]butanoic acid
0.045
3-deaza-S-adenosyl-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.008
8-aza-S-adenosyl-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.045
S-(1-deazaadenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0068
S-(3-deazaadenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.003
S-(8-azaadenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0067 - 0.029
S-(N-(2-biphenyl-4-ylethyl)-2-chloroadenosyl)-L-homocysteine
0.0006 - 0.0054
S-(N-(2-biphenyl-4-ylethyl)adenosyl)-L-homocysteine
0.0009 - 0.018
S-(N-(3,5-dimethoxybenzyl)adenosyl)-L-homocysteine
0.0036 - 0.022
S-(N-(pyridin-4-ylmethyl)adenosyl)-L-homocysteine
0.001 - 0.061
S-(N-benzyladenosyl)-L-homocysteine
0.002 - 0.154
S-(N-phenyladenosyl)-L-homocysteine
0.002 - 0.027
S-(N-phenylethyladenosyl)-L-homocysteine
0.0006 - 0.0074
S-(N-phenylpropyladenosyl)-L-homocysteine
0.05
S-adenosyl-L-ethionine
Triticum aestivum
-
IC50: 0.05 mM
0.0003 - 0.002
S-adenosyl-L-homocysteine
0.028 - 0.3
S-nebularinehomocysteine
0.0003 - 0.0015
S-Tubercidinylhomocysteine
0.0029
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-chloro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0572
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-chloro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0025
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-fluoro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0068
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-fluoro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.021
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.256
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.001
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methyl-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.006
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methyl-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.216
2-amino-4-[([(2S,3S,4R,5R)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-3,4-dihydroxytetrahydrofuran-2-yl]methyl)sulfanyl]butanoic acid
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.5
2-amino-4-[([(2S,3S,4R,5R)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-3,4-dihydroxytetrahydrofuran-2-yl]methyl)sulfanyl]butanoic acid
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.017
NSC 14778
Homo sapiens
-
isozyme Dnmt3b
0.092
NSC 14778
Homo sapiens
-
isozyme Dnmt1
0.0067
S-(N-(2-biphenyl-4-ylethyl)-2-chloroadenosyl)-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.029
S-(N-(2-biphenyl-4-ylethyl)-2-chloroadenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0006
S-(N-(2-biphenyl-4-ylethyl)adenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0054
S-(N-(2-biphenyl-4-ylethyl)adenosyl)-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0009
S-(N-(3,5-dimethoxybenzyl)adenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.018
S-(N-(3,5-dimethoxybenzyl)adenosyl)-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0036
S-(N-(pyridin-4-ylmethyl)adenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.022
S-(N-(pyridin-4-ylmethyl)adenosyl)-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.001
S-(N-benzyladenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.061
S-(N-benzyladenosyl)-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.002
S-(N-phenyladenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.154
S-(N-phenyladenosyl)-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.002
S-(N-phenylethyladenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.027
S-(N-phenylethyladenosyl)-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0006
S-(N-phenylpropyladenosyl)-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0074
S-(N-phenylpropyladenosyl)-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0003
S-adenosyl-L-homocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.002
S-adenosyl-L-homocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
1
S-inosinylhomocysteine
Homo sapiens
IC50 above 1 mM, isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
1
S-inosinylhomocysteine
Homo sapiens
IC50 above 1 mM, isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.028
S-nebularinehomocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.3
S-nebularinehomocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0003
S-Tubercidinylhomocysteine
Homo sapiens
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
0.0015
S-Tubercidinylhomocysteine
Homo sapiens
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37°C
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