Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein in Escherichia coli | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
NADPH | pH 7.0, 37°C | Gluconobacter oxydans | |
0.82 | - |
(E)-2-hexenal | pH 7.0, 37°C | Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39240 | - |
calculated from cDNA | Gluconobacter oxydans |
44000 | - |
SDS-PAGE | Gluconobacter oxydans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(E)-2-hexenal + NADPH | relative activity: 100% | Gluconobacter oxydans | hexanal + NADP+ | - |
? | |
(E)-3-nonen-2-one + NADPH | relative activity: 61.6% | Gluconobacter oxydans | nonan-2-one + NADP+ | - |
? | |
2,6,6-trimethyl-2-cyclohexen-1,4-dione + NADPH | relative activity: 103% | Gluconobacter oxydans | ? | - |
? | |
2-cyclohexen-1-one + NADPH | relative activity: 56.7% | Gluconobacter oxydans | ? | - |
? | |
2-methylcyclopenten-1-one + NADPH | relative activity: 12.4% | Gluconobacter oxydans | ? | - |
? | |
citral + NADPH | relative activity: 34% | Gluconobacter oxydans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoate reductase | - |
Gluconobacter oxydans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
only 25% residual activity | Gluconobacter oxydans |
37 | - |
assay at | Gluconobacter oxydans |
45 | - |
optimal activity | Gluconobacter oxydans |
53 | - |
only 25% residual activity | Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 30 | enzyme stable over 24 h | Gluconobacter oxydans |
59 | - |
midpoint thermal inactivation | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
optimal activity in TEA buffer | Gluconobacter oxydans |
7 | - |
optimal activity incitrate-phosphate buffer | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | required | Gluconobacter oxydans | |
NADH | - |
Gluconobacter oxydans | |
NADPH | NADPH is preferred, showing an 8fold higher specific activity | Gluconobacter oxydans |