Information on EC 1.3.1.31 - 2-enoate reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.3.1.31
-
RECOMMENDED NAME
GeneOntology No.
2-enoate reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
butanoate + NAD+ = but-2-enoate + NADH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenation
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oxidation
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redox reaction
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reduction
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Microbial metabolism in diverse environments
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Phenylalanine metabolism
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SYSTEMATIC NAME
IUBMB Comments
butanoate:NAD+ DELTA2-oxidoreductase
An iron-sulfur-flavoprotein (FAD). Acts (in the reverse direction) on a wide range of alkyl and aryl alphabeta-unsaturated carboxylate ions; but-2-enoate was the best substrate tested.
CAS REGISTRY NUMBER
COMMENTARY hide
70712-51-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
chicory
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain ATCC 8585D-5
UniProt
Manually annotated by BRENDA team
no activity in Clostridium butyricum
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-
Manually annotated by BRENDA team
no activity in Clostridium pasteurianum
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-
-
Manually annotated by BRENDA team
no activity in Clostridium propionicum
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Manually annotated by BRENDA team
xenobiotic reductase A showing enoate reductase activity
UniProt
Manually annotated by BRENDA team
various red yeasts
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Manually annotated by BRENDA team
Rhodotorula sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain ATCC 43970
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-carvone + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 100%, ee: 97%
-
-
?
(+)-costunolide + NADPH
11(S),13-dihydrocostunolide + NADP+
show the reaction diagram
(-)-carvone + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 100%, ee: 95%
-
-
?
(2E)-2-benzylidenecyclohexanone + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 92%, ee: 51%
-
-
?
(2E)-2-benzylidenecyclopentanone + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 4%, ee: racemate
-
-
?
(2E)-2-cyano-3-phenylprop-2-enoic acid + NADPH
2-cyano-3-phenylpropanoic acid + NADP+
show the reaction diagram
conversion rate measured using HPLC after 48 h. Conversion: 83%, ee: racemic
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-
?
(2E)-2-methyl-3-phenylacrylic acid + electron donor
2-methyl-3-phenylpropanoic acid + electron acceptor
show the reaction diagram
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-
-
-
?
(2E)-2-methyl-3-phenylbut-2-enoic acid + electron donor
2-methyl-3-phenylbutanoic acid + electron acceptor
show the reaction diagram
-
-
-
-
?
(2E)-2-methylbut-2-enedioic acid + electron donor
2-methylbutanedioic aicd + electron acceptor
show the reaction diagram
-
-
-
-
?
(2E)-3-phenylacrylic acid + electron donor
3-phenylpropanoic acid + electron acceptor
show the reaction diagram
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-
-
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?
(2E)-4-methoxy-2-methyl-4-oxobut-2-enoic acid + electron donor
4-methoxy-2-methyl-4-oxobutanoic acid + electron acceptor
show the reaction diagram
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-
-
-
?
(2E)-4-methoxy-3-methyl-4-oxobut-2-enoic acid + electron donor
4-methoxy-3-methyl-4-oxobutanoic acid + electron acceptor
show the reaction diagram
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-
-
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?
(2E,4E)-2,3,5,8-tetramethylnona-2,4,7-trienoic acid + electron donor
? + electron acceptor
show the reaction diagram
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-
-
-
?
(2E,4E)-2,3-dimethylhexa-2,4-dienoic acid + electron donor
? + electron acceptor
show the reaction diagram
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-
-
-
?
(2E,6Z)-nona-2,6-dienal + NADH + H+
?
show the reaction diagram
-
relative activity: 62.7%
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?
(2Z)-3-(4-chlorophenyl)-3-cyanoprop-2-enoic acid + NADPH
(R)-3-(4-chlorophenyl)-3-cyano-propanoic acid + NADP+
show the reaction diagram
conversion rate measured using HPLC after 48 h. Conversion: 80%, ee: 94% (R)
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(E)-2,5-dimethoxycinnamate + NADH
3-(2,5-dimethoxyphenyl)propionate + NAD+
show the reaction diagram
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-
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?
(E)-2-butenoate + NADH
butanoate + NAD+
show the reaction diagram
(E)-2-decenal + NADH + H+
decanal + NAD+
show the reaction diagram
-
relative activity: 90%
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-
?
(E)-2-hexen-1-al + NADH + H+
hexanal + NAD+
show the reaction diagram
-
relative activity: 100%
-
-
?
(E)-2-hexenal + NADPH
hexanal + NADP+
show the reaction diagram
-
relative activity: 100%
-
-
?
(E)-2-methyl-2-butenal + NADH + H+
2-methylbutanal + NADH
show the reaction diagram
-
relative activity: 59.2%
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?
(E)-2-methyl-2-butenoate + 4,5-dihydroxyanthraquinone-2-carboxylic acid
2-methylbutanoate + ?
show the reaction diagram
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-
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?
(E)-2-methyl-2-butenoate + NADH
2-methylbutanoate + NAD+
show the reaction diagram
(E)-2-methyl-3-phenyl-2-propenoate + reduced methyl viologen
(R)-2-methyl-3-phenyl-2-propanoate + methyl viologen
show the reaction diagram
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?
(E)-2-methylbutenoate + NADH
(R)-2-methylbutanoate + NAD+
show the reaction diagram
(E)-2-methylbutenoate + reduced methylviologen
(R)-2-methylbutanoate + methyl viologen
show the reaction diagram
(E)-2-nonenal + NADH + H+
nonanal + NAD+
show the reaction diagram
-
relative activity: 57%
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?
(E)-2-octenal + NADH + H+
octanal + NAD+
show the reaction diagram
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relative activity: 75.4%
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?
(E)-2-oxo-4-phenyl-3-butenoate + NADH
2-oxo-4-phenyl-3-butanoate + NAD+
show the reaction diagram
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18% of the activity with butenoate
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?
(E)-2-pentenal + NADH + H+
2-pentanone + NAD+
show the reaction diagram
-
relative activity: 45%
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?
(E)-3-methyl-2-oxo-4-phenyl-3-butenoate + NADH
3-methyl-2-oxo-4-phenyl-3-butenoate + NAD+
show the reaction diagram
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27% of the activity with butenoate
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?
(E)-3-methylpentenoate + NADH
3-methylpentanoate + NAD+
show the reaction diagram
(E)-3-nonen-2-one + NADH + H+
nonan-2-one + NAD+
show the reaction diagram
-
relative activity: 36.6%
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?
(E)-3-nonen-2-one + NADPH
nonan-2-one + NADP+
show the reaction diagram
-
relative activity: 61.6%
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?
(E)-4-methyl-2-pentenoate + NADH
4-methylpentanoate + NAD+
show the reaction diagram
(E)-cinnamate + NADH
phenylpropionate + NAD+
show the reaction diagram
(E)-geraniate + NADH
?
show the reaction diagram
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-
-
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?
(E)-o-hydroxycinnamate + NADH
3-(2-hydroxyphenyl)propionate + NAD+
show the reaction diagram
(E)-p-(dimethylamino)cinnamate + NADH
3-(4-(dimethylamino)phenyl)propionate + NAD+
show the reaction diagram
(E)-p-chlorocinnamate + NADH
3-(4-chlorophenyl)propionate + NAD+
show the reaction diagram
(E)-p-methoxycinnamate + NADH
3-(4-methoxyphenyl)propionate + NAD+
show the reaction diagram
(E)-p-nitrocinnamate + NADH
3-(4-nitrophenyl)propionate + NAD+
show the reaction diagram
(E,E)-2,4-hexadienoate + NADH
? + NAD+
show the reaction diagram
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-
-
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?
(R)-(-)-carvone + NADH + H+
?
show the reaction diagram
-
relative activity: 70%
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?
(R)-carvone + NADPH
2-methyl-5-(1-methylethenyl)cyclohexanone + NADP+
show the reaction diagram
(R,S)-1-methyl-3-phenylallenecarboxylate + NADH
? + NAD+
show the reaction diagram
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-
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?
(Z)-2-(formylamino)cinnamate + NADH
3-(4-(formylamino)phenyl)propionate + NAD+
show the reaction diagram
(Z)-2-bromocinnamate + NADH
3-(2-bromophenyl)propionate + NAD+
show the reaction diagram
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-
-
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?
(Z)-2-chloro-3-(p-chlorophenyl)acrylate + NADH
2-chloro-3-(4-chlorophenyl)propionate + NAD+
show the reaction diagram
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-
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?
(Z)-2-fluorocinnamate + NADH
3-(2-fluorophenyl)propionate + NAD+
show the reaction diagram
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?
(Z)-3-chlorocinnamate + NADH
3-(3-chlorophenyl)propionate + NAD+
show the reaction diagram
-
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?
(Z)-3-cyano-3-phenyl-propenoic acid + NADPH + H+
(R)-3-cyano-3-phenylpropanoic acid + NADP+
show the reaction diagram
highest enantiomeric excess of 98% ee is achieved at a glucose concentration of 1% (w/v) with a 47% conversion for an 18 h reaction. Concentration of 2-propanol also affects the activity and selectivity of the reaction, and a concentration of 5% (v/v) results in the best substrate conversion and ee value of product. Conversion rate measured using HPLC after 48 h. Conversion: 80%, ee: 98% (R)
-
-
?
(Z)-3-methylpentenoate + NADH
3-methylpentanoate + NAD+
show the reaction diagram
(Z)-geraniate + NADH
?
show the reaction diagram
-
-
-
-
?
1,3-dimethyl-1H-pyrrole-2,5-dione + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
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-
?
1-(cyclohex-1-en-1-yl)ethanone + NADPH
?
show the reaction diagram
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biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 71%, ee: not applicable
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-
?
1-(cyclopent-1-en-1-yl)ethanone + NADPH
?
show the reaction diagram
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biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 30%, ee: not applicable
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?
1-(prop-2-en-1-yl)-1H-pyrrole-2,5-dione + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: not applicable
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-
?
1-butyl-3-methyl-1H-pyrrole-2,5-dione + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
-
?
1-ethyl-3-methyl-1H-pyrrole-2,5-dione + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
-
?
12-phenyldodec-2-enoic acid + electron donor
12-phenyldodecanoic acid + electron acceptor
show the reaction diagram
-
-
-
-
?
2,3-dimethylmaleimide + NADH
trans-dimethylsuccinimide + NAD+
show the reaction diagram
-
-
-
-
?
2,6,6-trimethyl-2-cyclohexen-1,4-dione + NADPH
?
show the reaction diagram
-
relative activity: 103%
-
-
?
2-cyclohexen-1-one + NADH
2-cyclohexanone + NAD+
show the reaction diagram
2-cyclohexen-1-one + NADH + H+
2-cyclohexanone + NAD+
show the reaction diagram
-
-
-
-
?
2-cyclohexen-1-one + NADH + H+
cyclohexanone + NAD+
show the reaction diagram
-
relative activity: 830%
-
-
?
2-cyclohexen-1-one + NADPH
?
show the reaction diagram
-
relative activity: 56.7%
-
-
?
2-isopropyl-5-methyl-2-hexenal + NADH + H+
2-isopropyl-5-methylhexanal + NAD+
show the reaction diagram
-
relative activity: 15.5%
-
-
?
2-methyl-2-butenoate + NADH
2-methylbutanoate + NAD+
show the reaction diagram
2-methyl-2-cyclopenten-1-one + NADH + H+
?
show the reaction diagram
-
relative activity: 62.7%
-
-
?
2-methyl-N-phenylmaleimide + NADH + H+
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+
show the reaction diagram
2-methyl-N-phenylmaleimide + NADPH
(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+
show the reaction diagram
2-methylbutenoate + NADH
2-methylbutanoate + NAD+
show the reaction diagram
-
-
-
-
?
2-methylcyclopent-2-enone + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 92%, ee: 52%
-
-
?
2-methylcyclopenten-1-one + NADPH
?
show the reaction diagram
-
relative activity: 12.4%
-
-
?
2-methylenesuccinic acid + NAD(P)H
(R)-2-methylsuccinic acid + NAD(P)+
show the reaction diagram
2-methylfumaric acid + NAD(P)H
2-methylsuccinic acid + NAD(P)+
show the reaction diagram
2-methylmaleic acid + NAD(P)H
(R)-2-methylsuccinic acid + NAD(P)+
show the reaction diagram
3-methyl-1-(prop-2-en-1-yl)-1H-pyrrole-2,5-dione + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
-
?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH
(R)-N-phenyl-2-methylsuccinimide + NADP+
show the reaction diagram
conversion rate measured using HPLC after 48 h. Conversion: 99%, ee: 99% (R)
-
-
?
3-methyl-1-propyl-1H-pyrrole-2,5-dione + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
-
?
3-methyl-2-butenal + NADPH
3-methylbutanal + NADP+
show the reaction diagram
3-methyl-2-cyclopentenone + NADH + H+
?
show the reaction diagram
-
relative activity: 31%
-
-
?
3-methylpentenoate + NADH
3-methylpentanoate + NAD+
show the reaction diagram
5-benzylidenethiazolidine-2,4-dione + NADH
(5R)-5-benzyl-1,3-thiazolidine-2,4-dione + NAD+
show the reaction diagram
alpha-methylmaleimide + NADH
(R)-2-methylsuccinimide + NAD+
show the reaction diagram
-
-
-
-
?
bromomaleic anhydride + NADH + H+
?
show the reaction diagram
-
relative activity: 107%
-
-
?
but-3-en-2-one + NADPH
butan-2-one + NADP+
show the reaction diagram
butenoate + NADH
butanoate + NAD+
show the reaction diagram
butenoate + reduced methyl viologen
butanoate + methyl viologen
show the reaction diagram
-
-
-
-
-
chloroacrylic acid + NADPH
(S)-2-chloropropionic acid + NADP+
show the reaction diagram
-
-
-
-
?
cinnamaldehyde + NADPH
?
show the reaction diagram
cinnamate + NADH
phenylpropionate + NAD+
show the reaction diagram
citraconic anhydride + NADH
?
show the reaction diagram
-
-
-
-
?
citral + NADPH
3-phenylpropanal + NADP+
show the reaction diagram
citral + NADPH
?
show the reaction diagram
-
relative activity: 34%
-
-
?
diethyl (2Z)-2-methylbut-2-enedioate + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 99%
-
-
?
dimethyl (2E)-2-methylbut-2-enedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + dimethyl (2S)-2-methylbutanedioate + NAD(P)+
show the reaction diagram
dimethyl (2Z)-2-methylbut-2-enedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + NAD(P)+
show the reaction diagram
dimethyl (2Z)-2-methylbut-2-enedioate + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 99%, ee: 98%
-
-
?
dimethyl 2-methylidenebutanedioate + NAD(P)H
dimethyl (2R)-2-methylbutanedioate + NAD(P)+
show the reaction diagram
dimethyl 2-methylidenebutanedioate + NADPH
?
show the reaction diagram
-
biotransformation in the presence of a NADP+/G6P/G6PDH cofactor recycling system, conversion: 19%, ee: 90%
-
-
?
ethyl (2Z)-3-nitro-2-phenylprop-2-enoate + NADPH
ethyl (2R)-3-nitro-2-phenylpropanoate + NADP+
show the reaction diagram
conversion rate measured using HPLC after 48 h. Conversion: 40%, ee: 45% (R)
-
-
?
ethyl 3-(tetrahydrofuran)propanoate + NADP+
? + NADPH
show the reaction diagram
-
-
-
-
?
ethyl 3-(tetrahydrofuran)propanoate + NADPH
? + NADP+
show the reaction diagram
hexa-2,4-dienoic acid + electron donor
? + electron acceptor
show the reaction diagram
-
-
-
-
?
indolylacrylate + NADH
indolepropionate + NAD+
show the reaction diagram
-
-
-
-
?
ketoisophorone + NAD(P)H
(6R)-levodione + NAD(P)+
show the reaction diagram
-
-
-
-
?
ketoisophorone + NADPH
(6R)-levodione
show the reaction diagram
maleic anhydride + NADPH
dihydrofuran-2,5-dione + NADP+
show the reaction diagram
maleimide + NADH
pyrrolidine-2,5-dione + NADH
show the reaction diagram
monomethyl fumarate + NADH
4-methoxy-4-oxobutanoate + NAD+
show the reaction diagram
-
-
-
-
?
N-ethylmaleimide + NADPH
N-ethylpyrrolidine-2,5-dione + NADH
show the reaction diagram
oct-2-en-4-ynoic acid + electron donor
? + electron acceptor
show the reaction diagram
-
-
-
-
?
phenylmaleic anhydride + NADH + H+
?
show the reaction diagram
-
relative activity: 402%
-
-
?
trans,trans-2,4-hexadienal + NADPH
?
show the reaction diagram
[(1E)-1-nitroprop-1-en-2-yl]benzene + NADPH
(S)-1-nitro-2-phenylpropane + NADP+
show the reaction diagram
conversion rate measured using HPLC after 48 h. Conversion: 87%, ee: 97% (S)
-
-
?
[(1E)-2-nitroprop-1-en-1-yl]benzene + NADPH
1-phenyl-2-nitropropane + NADP+
show the reaction diagram
conversion rate measured using HPLC after 48 h. Conversion: 60%, ee: racemic
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-costunolide + NADPH
11(S),13-dihydrocostunolide + NADP+
show the reaction diagram
-
part of the metabolization of (+)-costunolide to leucodin, overview
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD(P)H
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-cyclohexen-1-one
-
the addition of 260 mM 2-cyclohexen-1-one leads to a more than 30fold decrease of the half-life at 30C
2-methylbutenoate
-
in all reversed micellar solutions inhibition by the enoate is observed at an overall concentration of 0.5-5 mM, implying that a concentration of substrate equal to the Km value in aqueous solution may already cause inhibition in reversed micelles
3-Phenylpropionate
4-phenylbutanoate
Aliphatic carboxylates
-
-
aminobenzotriazole
-
slight inhibition
Benzoate
dicoumarol
dimethyl (2E)-2-methylbut-2-enedioate
-
-
dimethyl (2R)-2-methylbutanedioate
-
-
dimethyl (2S)-2-methylbutanedioate
-
-
dimethyl (2Z)-2-methylbut-2-enedioate
-
-
dimethyl 2-methylidenebutanedioate
-
-
fumarate
Mersalyl
-
1 mM, complete inhibition
methyl viologen
-
-
Metyrapone
-
slight inhibition
O2
-
in the reduced state the enzyme is inactivated in 1-2 min, about 3 h after the removal of oxygen, the activity is partially restored
p-hydroxymercuribenzoate
-
0.1 mM, complete inhibition
phenyl propionate
-
-
phenylacetate
reduced methyl viologen
-
-
additional information
-
not inhibited by 2-cyclohexanone
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
argon
-
34% activation
clotrimazole
-
slight activation
CO
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atmosphere of 80% v/v with 20% v/v O2, 40% activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.029
(2E)-2-methyl-3-phenylacrylic acid
-
-
0.037
(2E)-2-methyl-3-phenylbut-2-enoic acid
-
-
7.4
(2E)-2-methylbut-2-enedioic acid
-
-
0.15
(2E)-3-phenylacrylic acid
-
-
0.8
(E)-2-butenoate
-
-
0.82
(E)-2-hexenal
-
pH 7.0, 37C
1.67
(E)-2-methyl-2-butenoate
-
-
0.014
(E)-geraniate
-
-
0.5
(Z)-2-bromocinnamate
-
-
-
0.1
(Z)-geraniate
-
-
0.01
12-phenyldodec-2-enoic acid
-
-
1.94
2-cyclohexen-1-one
-
pH 7.0, 30C
0.0014
2-methyl-N-phenylmaleimide
pH 7.5, 30C
6
2-methylbutenoate
-
in 50 mM HEPES, pH 7.0, ionic strength 0.05 M
1.7
4-methoxy-2-methyl-4-oxobutanoic acid
-
-
16.2
4-methoxy-3-methyl-4-oxobutanoic acid
-
-
1.5
butenoate
-
-
0.013 - 0.02
cinnamate
0.012 - 0.034
NADH
0.0854 - 1.24
NADPH
1.4
oct-2-en-4-ynoic acid
-
-
0.4
reduced methyl viologen
-
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8400
2-cyclohexen-1-one
Lactobacillus casei
-
pH 7.0, 30C
5.9
2-methyl-N-phenylmaleimide
Achromobacter sp.
I3V5V6
pH 7.5, 30C
38.6 - 3200
NADH
1.97 - 19.2
NADPH
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.4
2-methyl-N-phenylmaleimide
Achromobacter sp.
I3V5V6
pH 7.5, 30C
29151
2000
NADH
Achromobacter sp.
I3V5V6
pH 7.5, 30C
8
220
NADPH
Achromobacter sp.
I3V5V6
pH 7.5, 30C
5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20
methyl viologen
-
-
30
phenyl propionate
-
-
10
reduced methyl viologen
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.46
highest specific activity with 10 mM N-ethylmaleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25C
15.81
-
highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25C
20
-
(E)-2-methylbutenoate
25.82
highest specific activity with 10 mM maleimide in 200 mM sodium phosphate, at pH 7.5, 0.2 mM NADPH, at 25C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
optimal activity in TEA buffer
7.5
highest activity using Tris-HCl
8
-
assay at
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 6.2
-
about 65% of maximal activity at pH 4.5 and at pH 6.2
5 - 9
active between pH 5.0 and pH 9.0
6
conversion rate drops to 20% at pH 6.0
7
highest conversion rate 54%, pH optimum
8
48% conversion rate
8 - 9
-
optimal pH range
9
only 30% of highest activity at pH 9
additional information
no activity at pH 11
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28
-
assay at
35
optimal temperature in potassium phosphate buffer
45
-
optimal activity
53
-
only 25% residual activity
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
about 40% of maximal activity maintained
45
about 40% of maximal activity maintained
50 - 55
-
temp. optimum
55
no activity beyond 55C
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
39240
-
calculated from cDNA
40600
calculated from cDNA
42300
-
calculated from cDNA
44000
-
SDS-PAGE
46800
-
SDS-PAGE
170000
-
gel filtration
450000
-
gel filtration
600000
gel filtration
800000
-
gel filtration
940000
-
equilibrium ultracentrifugation
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 73000, SDS-PAGE
dodecamer
-
12 * 70000, SDS-PAGE; 12 * 73000, equilibrium sedimentation in guanidine hydrochloride
hexamer
-
6 * 78000, SDS-PAGE
tetramer
-
4 * 42300, gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9.5
-
the activity drops off sharply between pH 5-6 and pH 8.5-9.5
684248
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 30
-
enzyme stable over 24 h
30
half-life of inactivation: 64.8 h
30 - 45
55
-
20 min, stable
59
-
midpoint thermal inactivation
65
-
no activity after 1 h
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme immobilized in calcium alginate gel, used in operation for 2 weeks, half-life of about 200 h
-
half-life of 150 h during operation, in the immobilized form on filter paper. Half-life: 350 h, electrode immobilized enzyme
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
relatively stable in presence of oxygen, 50% loss of activity after 30 h
-
390672
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18C, pH 7, buffer containing 10 mM phosphate and 0.1 M KCl, formation of a dissociation product sedimenting at about 10 S
-
-20C, phosphate buffer, pH 7.0, containing (E)-2-methyl-2-butenoate, EDTA, sucrose and 2-mercaptoethanol, repeatedly thawed and frozen, 50% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni2+-NTA bead chromatography
recombinant enzyme
using affinity chromatography
using ammonium sulfate precipitation and treatment in an acidic buffer
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli BL21
-
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli growing under anaerobic conditions
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
using Ni-NTA chromatography
-