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Literature summary for 1.15.1.1 extracted from

  • Zhu, Y.; Wang, G.; Ni, H.; Xiao, A.; Cai, H.
    Cloning and characterization of a new manganese superoxide dismutase from deep-sea thermophile Geobacillus sp. EPT3 (2013), World J. Microbiol. Biotechnol., 30, 1347-1357.
    View publication on PubMed
Search for more literature for 1.15.1.1

Cloned(Commentary)

Cloned (Comment) Organism
gene sod, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Geobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
 Geobacillus sp.
Ba2+ inhibitory at 1 mM Geobacillus sp.
Ca2+ inhibitory at 1 mM Geobacillus sp.
CHAPS
-
 Geobacillus sp.
Co2+ inhibitory at 1 mM Geobacillus sp.
Cu2+ 50% inhibition at 1 mM Geobacillus sp.
dithiothreitol
-
 Geobacillus sp.
EDTA strong inhibition at 10 mM Geobacillus sp.
Fe2+ inhibitory at 1 mM Geobacillus sp.
guanidine hydrochloride
-
 Geobacillus sp.
Mg2+ inhibitory at 1 mM Geobacillus sp.
additional information insensitivity of the recombinant enzyme to both KCN and H2O2 Geobacillus sp.
Ni2+ inhibitory at 1 mM Geobacillus sp.
phenylmethylsulfonyl fluoride
-
 Geobacillus sp.
SDS
-
 Geobacillus sp.
Triton X-100
-
 Geobacillus sp.
Tween 20
-
 Geobacillus sp.
Urea
-
 Geobacillus sp.
Zn2+ inhibitory at 1 mM Geobacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ a Mn-SOD, Mn2+ activates, the manganese-binding site is formed by conserved residues His260, His308, Asp392, and His396 Geobacillus sp.
additional information no enzyme activity with Fe2+-reconstituted enzyme Geobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50230
-
 2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation Geobacillus sp.
59000
-
 2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation Geobacillus sp.
130700
-
 recombinant His-tagged enzyme, gel filtration Geobacillus sp.

Organism

Organism UniProt Comment Textmining
Geobacillus sp. T1T1K2 gene sod
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Geobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1204
-
 purified recombinant His-tagged enzyme, pH 7.8, 25°C Geobacillus sp.

Subunits

Subunits Comment Organism
homodimer 2 * 59000, SDS-PAGE, 2 * 50230, sequence calculation Geobacillus sp.
More modelling of the three-dimensional structure of SOD monomer, overview Geobacillus sp.

Synonyms

Synonyms Comment Organism
SOD
-
 Geobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Geobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified recombinant enzyme, stable Geobacillus sp.
80
-
 purified recombinant enzyme, 1 h, 64% activity remaining Geobacillus sp.
90
-
 purified recombinant enzyme, 1 h, 57% activity remaining Geobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Geobacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 purified recombinant enzyme, stable Geobacillus sp.

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus sp. sequence calculation
-
 4.65

General Information

General Information Comment Organism
additional information Geobacillus sp. EPT3 SOD contains the LPPLPYRYDALEP sequence, which is identical to the conserved amino acid sequence (LPXLPYXXXXLEP) found at the N-terminal region of many Mn-SODs Geobacillus sp.