Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Phenylthiourea | binding and inhibition mode | Streptomyces antibioticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, the activation free energy is dominated by the enthalpic term, the value of which lies in the relatively narrow range of 61 kJmol-1 irrespective of substrate or reaction type as monophenolase, EC 1.14.18.1, or diphenolase | Streptomyces antibioticus | |
0.22 | - |
4-hydroxyphenylpropionic acid | pH 6.8, 10°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
0.28 | - |
methyl 3-hydroxy-L-tyrosinate | pH 6.8, 10°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
0.38 | - |
methyl 3-hydroxy-L-tyrosinate | pH 6.8, 25°C, in aqueous Hepes buffer | Streptomyces antibioticus | |
0.4 | - |
dopamine | pH 6.8, 10°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
0.44 | - |
4-hydroxyphenylpropionic acid | pH 6.8, 25°C, in aqueous Hepes buffer | Streptomyces antibioticus | |
0.51 | - |
tyramine | pH 6.8, 25°C, in aqueous Hepes buffer | Streptomyces antibioticus | |
0.66 | - |
tyramine | pH 6.8, 10°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
0.8 | - |
L-Dopa | pH 6.8, 25°C, in aqueous Hepes buffer | Streptomyces antibioticus | |
0.82 | - |
methyl 3-hydroxy-L-tyrosinate | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
0.93 | - |
dopamine | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
2.1 | - |
L-Dopa | pH 6.8, 10°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
2.1 | - |
tyramine | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
2.2 | - |
dopamine | pH 6.8, 25°C, in aqueous Hepes buffer | Streptomyces antibioticus | |
3.7 | - |
L-Dopa | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
6.7 | - |
4-hydroxyphenylpropionic acid | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | dependent on, type-3 copper enzyme | Streptomyces antibioticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces antibioticus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-tyrosine + O2 = dopaquinone + H2O | the active site of the enzyme contains a pair of antiferromagnetically coupled Cu2+ ions in the met form, and during turnover both the reduced and the oxy forms are cyclically produced, the rate-limiting step is associated with cleavage of the peroxide O-O bond, reaction and kinetic mechanism | Streptomyces antibioticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpropionic acid + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
dopamine + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
L-DOPA + O2 | - |
Streptomyces antibioticus | ? | - |
? | |
L-tyrosine methyl ester + O2 + AH2 | - |
Streptomyces antibioticus | L-dopa methyl ester + H2O + A | - |
? | |
methyl 3-hydroxy-L-tyrosinate + O2 | - |
Streptomyces antibioticus | ? | - |
r | |
additional information | activity with phenolic and diphenolic substrates, also performing the reaction of tyrosinase, a ortho-hydroxylation of monophenols, EC 1.14.18.1, and the oxidation of catechols to ortho-quinones, the diphenolase activity, EC 1.10.3.1, overview | Streptomyces antibioticus | ? | - |
? | |
tyramine + O2 | - |
Streptomyces antibioticus | 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
diphenolase | - |
Streptomyces antibioticus |
More | cf. EC 1.10.3.1 | Streptomyces antibioticus |
tyrosinase | - |
Streptomyces antibioticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 25 | assay at | Streptomyces antibioticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-Dopa | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
1.9 | - |
tyramine | pH 6.8, 10°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
2.24 | - |
methyl 3-hydroxy-L-tyrosinate | pH 6.8, 10°C, in methanolglycerol cryosolvent | Streptomyces antibioticus | |
3.4 | - |
methyl 3-hydroxy-L-tyrosinate | pH 6.8, 25°C, in aqueous buffer | Streptomyces antibioticus | |
7.4 | - |
tyramine | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
8.9 | - |
methyl 3-hydroxy-L-tyrosinate | pH 6.8, 25°C, in methanolglycerol cryosolvent | Streptomyces antibioticus | |
25.9 | - |
tyramine | pH 6.8, 25°C, in aqueous buffer | Streptomyces antibioticus | |
29.9 | - |
dopamine | pH 6.8, 10°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
42 | - |
L-Dopa | pH 6.8, 10°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
43 | - |
4-hydroxyphenylpropionic acid | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
66.7 | - |
4-hydroxyphenylpropionic acid | pH 6.8, 25°C, in aqueous buffer | Streptomyces antibioticus | |
107.4 | - |
L-Dopa | pH 6.8, 25°C, in aqueous buffer | Streptomyces antibioticus | |
133.2 | - |
dopamine | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
141.1 | - |
L-Dopa | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
433 | - |
dopamine | pH 6.8, 25°C, in methanol-glycerol cryosolvent | Streptomyces antibioticus | |
439 | - |
dopamine | pH 6.8, 25°C, in aqueous buffer | Streptomyces antibioticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Streptomyces antibioticus |