Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant His-tagged Cat1.4 isozymes in Escherichia coli strain BL21 (DE3) | Bacillus anthracis |
Protein Variants | Comment | Organism |
---|---|---|
D270A | site-directed mutagenesis | Bacillus anthracis |
H146A | site-directed mutagenesis | Bacillus anthracis |
H215A | site-directed mutagenesis | Bacillus anthracis |
H55A | site-directed mutagenesis | Bacillus anthracis |
N376A | site-directed mutagenesis | Bacillus anthracis |
N82A | site-directed mutagenesis | Bacillus anthracis |
P391A | site-directed mutagenesis | Bacillus anthracis |
Q332A | site-directed mutagenesis | Bacillus anthracis |
R6A | site-directed mutagenesis | Bacillus anthracis |
V210A | site-directed mutagenesis | Bacillus anthracis |
W257A | site-directed mutagenesis | Bacillus anthracis |
Y117A | site-directed mutagenesis | Bacillus anthracis |
Y338A | site-directed mutagenesis | Bacillus anthracis |
Y397A | site-directed mutagenesis | Bacillus anthracis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.74 | - |
H2O2 | recombinant wild-type enzyme, pH 7.0, 25°C | Bacillus anthracis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | heme | Bacillus anthracis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, recombinant isozyme Cat1.4, SDS-PAGE | Bacillus anthracis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | Bacillus anthracis | - |
O2 + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | - |
isozyme Cat1.4 | - |
Purification (Comment) | Organism |
---|---|
recombinanat wild-type and mutant His-tagged Cat1.4 isozymes from Escherichia coli strain BL21 (DE3) to homogeneity | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 H2O2 | - |
Bacillus anthracis | O2 + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, recombinant isozyme Cat1.4, SDS-PAGE | Bacillus anthracis |
More | homology modeling of isozyme Cat1.4 | Bacillus anthracis |
Synonyms | Comment | Organism |
---|---|---|
Cat1.4 | - |
Bacillus anthracis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus anthracis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus anthracis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Bacillus anthracis |
General Information | Comment | Organism |
---|---|---|
additional information | structure-function analysis, overview. H55 and Y338 in the active site are crucial for the activity. The distal heme ligand binding domain 46RERIPERVVHAKG58 encompasses the essential distal histidine residue, and the proximal heme ligand binding domain 334R-F-Y-D340 harbors the essential proximal tyrosine residue. Other catalase specific motifs are 126VGNNTP131, 107RDXRGFAXKFYT118, and 92RFSTV96. Tyr117 from sequence 107RDXRGFAXKFYT118 is crucial for activity | Bacillus anthracis |
physiological function | catalase is responsible for the enzymatic destruction/detoxification of hydrogen peroxide, to combat its deleterious effects | Bacillus anthracis |