Information on EC 1.11.1.6 - catalase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
1.11.1.6
-
RECOMMENDED NAME
GeneOntology No.
catalase
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
ethanol degradation IV
-
Glyoxylate and dicarboxylate metabolism
-
methanol oxidation to formaldehyde IV
-
superoxide radicals degradation
-
Tryptophan metabolism
-
SYSTEMATIC NAME
IUBMB Comments
hydrogen-peroxide:hydrogen-peroxide oxidoreductase
A hemoprotein. A manganese protein containing MnIII in the resting state, which also belongs here, is often called pseudocatalase. The enzymes from some organisms, such as Penicillium simplicissimum, can also act as a peroxidase (EC 1.11.1.7) for which several organic substances, especially ethanol, can act as a hydrogen donor. Enzymes that exhibit both catalase and peroxidase activity belong under EC 1.11.1.21, catalase-peroxidase.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ab-catalase
A5XB38
-
BNC
-
-
-
caperase
-
-
-
-
CAT
-
-
-
-
CAT
Geomyces pannorum
-
-
CAT
Penicillium cyclopium 1
-
-
-
CAT
Penicillium islandicum
-
-
CAT
Penicillium piceum
-
-
CAT
Penicillium piceum F-648
-
-
-
CAT
Sclerotium sp.
-
-
CAT
-
-
CAT-1
Q9C168
-
CAT-A
A3REN3
-
CAT-A
Penicillium piceum
-
-
CAT-P
Penicillium piceum
-
-
CatA
Q6RSH8
monofunctional catalase
catalase
Colwellia sp.
-
-
catalase
Colwellia sp. MH2
-
-
-
catalase
-
-
catalase
A9GRH4
-
catalase
Phaeobacter gallaeciensis BS107
A9GRH4
-
-
catalase
Pibocella sp.
-
-
catalase
Pibocella sp. MH3
-
-
-
catalase
Roseibacterium elongatum OCh 323
-
-
-
catalase
Roseisalinus antarcticus EL-88
-
-
-
catalase
Sulfitobacter sp.
-
-
catalase
Sulfitobacter sp. MH1
-
-
-
catalase
-
-
catalase
Wenxinia marina HY34
-
-
-
catalase
-
-
catalase -peroxidase KatG
-
-
catalase A
Q6RSH8
monofunctional catalase
catalase C
Q6RSH8
a bifunctional catalase-peroxidase
catalase form III
-
-
catalase P
Q6RSH8
monofunctional catalase
catalase P
Paracoccidioides brasiliensis ATCC MYA-826, Paracoccidioides brasiliensis Pb01 ATCC-MYA-826
Q8X220
-
-
catalase-1
Q9C168
-
catalase-A
A3REN3
-
catalase-peroxidase
-
-
-
-
catalase-peroxidase
-
-
catalase-peroxidase
-
-
-
catalase-peroxidase
-
-
catalase-peroxidase
Q939D2
-
catalase-peroxidase
-
-
catalase-peroxidase
-
-
catalase-peroxidase
-
-
catalase-peroxidase
Mycobacterium sp. JC1, Mycobacterium sp. JC1 DSM 3803
-
-
-
catalase-peroxidase
-
-
catalase-peroxidase
-
; bifunctional enzyme with activities of EC 1.11.1.6 and EC 1.11.1.7
catalase-peroxidase
-
bifunctional enzyme with activities of EC 1.11.1.6 and EC 1.11.1.7
catalase-peroxidase
Q08129
-
catalase-peroxidase
-
-
catalase-peroxidase
-
-
catalase-peroxidase
-
bifunctional enzyme with activities of EC 1.11.1.6 and EC 1.11.1.7
catalase-peroxidase
-
-
catalase-phenol oxidase
-
bifunctional enzyme, its major activity is the catalase-mediated decomposition of hydrogen peroxide, but it also catalyzes phenol oxidation
catalase/peroxidase
A4QUT2, A4R5S9
-
catalase/peroxidase
Magnaporthe grisea MA 829
A4QUT2, A4R5S9
-
-
CATC
Q6RSH8
a bifunctional catalase-peroxidase
CatP
Q6RSH8
monofunctional catalase
CatP
Paracoccidioides brasiliensis ATCC MYA-826
Q8X220
-
-
CATPO
Scytalidium thermophilum ATCC 16454
-
-
-
CP
Mycobacterium sp. JC1, Mycobacterium sp. JC1 DSM 3803
-
-
-
equilase
-
-
-
-
H2O2:H2O2 oxidoreductase
-
-
H2O2:H2O2 oxidoreductase
-
-
H2O2:H2O2 oxidoreductase
-
-
H2O2:H2O2 oxidoreductase
-
-
haem catalase
L8B3D7
-
haem catalase
Bifidobacterium asteroides JCM 8230
L8B3D7
-
-
HPI-A
-
catalase-peroxidase isoenzyme
HPI-B
-
catalase-peroxidase isoenzyme
HPII
-
monofunctional catalase
HTHP
Q5LLG6
peroxidase and catalase activity
hydrogen peroxide oxidoreductase
-
-
Kat E catalase
-
-
KatA
Helicobacter pylori 26695, Helicobacter pylori Taiwanese TW-34
-
-
-
KatG
A4QUT2, A4R5S9
-
KatG
Magnaporthe grisea MA 829
A4QUT2, A4R5S9
-
-
KatG
-
; bifunctional enzyme with activities of EC 1.11.1.6 and EC 1.11.1.7
KatG
-
bifunctional enzyme with activities of EC 1.11.1.6 and EC 1.11.1.7
KatG
-
bifunctional enzyme with activities of EC 1.11.1.6 and EC 1.11.1.7
KatG1
A4R5S9
located intracellularly
KatG1
Magnaporthe grisea MA 829
A4R5S9
located intracellularly
-
KatG2
A4QUT2
located extracellularly
KatG2
Magnaporthe grisea MA 829
A4QUT2
located extracellularly
-
KpA
Klebsiella pneumoniae IFO 14940
-
-
-
manganese catalase
A8CFD3
-
manganese catalase
Q6LA34
-
optidase
-
-
-
-
PktA
Psychrobacter piscatorii T-3
B3FXQ9
-
-
polyethylene glycol-catalase
-
-
-
-
tyrosine-coordinated heme protein
Q5LLG6
-
VktA
Q9FAZ3
-
manganese catalase
Pyrobaculum calidifontis VA1
Q6LA34
-
-
additional information
-
enzyme belongs to the family of monofunctional catalases
CAS REGISTRY NUMBER
COMMENTARY
9001-05-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
enzyme expression is induced under phosphate-limiting growth conditions
-
-
Manually annotated by BRENDA team
isozyme Cat1.4
-
-
Manually annotated by BRENDA team
strain OF4
-
-
Manually annotated by BRENDA team
Bacillus firmus OF4
strain OF4
-
-
Manually annotated by BRENDA team
strain ATCC 14581
-
-
Manually annotated by BRENDA team
strain 13
-
-
Manually annotated by BRENDA team
strain N2a
-
-
Manually annotated by BRENDA team
strain 13
-
-
Manually annotated by BRENDA team
strain N2a
-
-
Manually annotated by BRENDA team
Bacillus subtilis KatA
KatA
Uniprot
Manually annotated by BRENDA team
Bacillus subtilis KatA
KatA
-
-
Manually annotated by BRENDA team
Beta vulgaris var. cicla
-
-
-
Manually annotated by BRENDA team
originally isolated from honeybee intestine, gene katA
UniProt
Manually annotated by BRENDA team
Bifidobacterium asteroides JCM 8230
originally isolated from honeybee intestine, gene katA
UniProt
Manually annotated by BRENDA team
commercial preparation
-
-
Manually annotated by BRENDA team
Brassica oleracea gongylodes, kohlrabi
-
-
Manually annotated by BRENDA team
three enzyme isoforms
-
-
Manually annotated by BRENDA team
bifunctional catalase-peroxidase KatG
UniProt
Manually annotated by BRENDA team
bifunctional catalase-peroxidase KatG
-
-
Manually annotated by BRENDA team
gene katG, bifunctional enzyme KatG with catalase and peroxidase activities
SwissProt
Manually annotated by BRENDA team
Capra capra
goat
-
-
Manually annotated by BRENDA team
Colwellia sp.
strain MH2
-
-
Manually annotated by BRENDA team
Colwellia sp. MH2
strain MH2
-
-
Manually annotated by BRENDA team
Pacific oyster
-
-
Manually annotated by BRENDA team
Cucurbita sp.
pumpkin
-
-
Manually annotated by BRENDA team
microfungus isolated from soil collected in arctic tundra
-
-
Manually annotated by BRENDA team
isoform CatA
-
-
Manually annotated by BRENDA team
isoform CatB
-
-
Manually annotated by BRENDA team
bifunctional catalase-peroxidase KatG
-
-
Manually annotated by BRENDA team
gene katE
-
-
Manually annotated by BRENDA team
strain K12
-
-
Manually annotated by BRENDA team
strain O157:H7, gene katP
-
-
Manually annotated by BRENDA team
Escherichia coli K12
strain K12
-
-
Manually annotated by BRENDA team
LLR02022, contains a catalase and a bifunctional catalase-peroxidase
-
-
Manually annotated by BRENDA team
Geomyces pannorum
microfungus isolated from soil collected in arctic tundra
-
-
Manually annotated by BRENDA team
bifunctional catalase-peroxidase KatG
-
-
Manually annotated by BRENDA team
cv. Fructidor
-
-
Manually annotated by BRENDA team
catalase activity in an msr strain is one-half that of the parent strain. This difference is only observed under oxidative stress conditions, and the activity is restored to nearly wild-type levels by adding methionine sulfoxide reductase plus dithiothreitol to msr strain extracts
-
-
Manually annotated by BRENDA team
strain 26695, gene katA, enzyme KatA
-
-
Manually annotated by BRENDA team
strain Taiwanese TW-34, gene katA, enzyme KatA
-
-
Manually annotated by BRENDA team
strain 26695, gene katA, enzyme KatA
-
-
Manually annotated by BRENDA team
Helicobacter pylori Taiwanese TW-34
strain Taiwanese TW-34, gene katA, enzyme KatA
-
-
Manually annotated by BRENDA team
patients with acne vulgaris
-
-
Manually annotated by BRENDA team
strain IFO 14940
-
-
Manually annotated by BRENDA team
Klebsiella pneumoniae IFO 14940
strain IFO 14940
-
-
Manually annotated by BRENDA team
Kloeckera sp.
strain 2201
-
-
Manually annotated by BRENDA team
Kloeckera sp. 2201
strain 2201
-
-
Manually annotated by BRENDA team
recombinant enzyme
-
-
Manually annotated by BRENDA team
Listeria monocytogenes F2365
-
-
-
Manually annotated by BRENDA team
strain MA 829
UniProt
Manually annotated by BRENDA team
Magnaporthe grisea MA 829
strain MA 829
UniProt
Manually annotated by BRENDA team
ATCC 51441, ATCC 51442, and ATCC 51440
-
-
Manually annotated by BRENDA team
microfungus isolated from soil collected in arctic tundra
-
-
Manually annotated by BRENDA team
microfungus isolated from soil collected in arctic tundra, 2 strains
-
-
Manually annotated by BRENDA team
Balb/c mice
-
-
Manually annotated by BRENDA team
male C57BL/6 mice and type II diabetic mice
-
-
Manually annotated by BRENDA team
Mus musculus BALB/c
Balb/c mice
-
-
Manually annotated by BRENDA team
strain JC1, DSM 3803
-
-
Manually annotated by BRENDA team
Mycobacterium sp. JC1
strain JC1, DSM 3803
-
-
Manually annotated by BRENDA team
; enzyme KatG is a bifunctional
-
-
Manually annotated by BRENDA team
bifunctional catalase-peroxidase KatG
-
-
Manually annotated by BRENDA team
clinical isolates from Malatya, Turkey
-
-
Manually annotated by BRENDA team
enzyme KatG, gene katG
-
-
Manually annotated by BRENDA team
microfungus isolated from soil collected in arctic tundra, 2 strains
-
-
Manually annotated by BRENDA team
isoform Cat-2
SwissProt
Manually annotated by BRENDA team
isoforms Cat-1, Cat-2, Cat-3
-
-
Manually annotated by BRENDA team
no activity in Amphiprora kufferathii
-
-
-
Manually annotated by BRENDA team
subspecies Oceanobacillus oncorhynchi incaldaniensis, strain 20AG
-
-
Manually annotated by BRENDA team
CAT-A
A3REN3
UniProt
Manually annotated by BRENDA team
isoform catalase-B
UniProt
Manually annotated by BRENDA team
Pb01, ATCC-MYA-826
SwissProt
Manually annotated by BRENDA team
strain ATCC MYA-826
UniProt
Manually annotated by BRENDA team
Paracoccidioides brasiliensis ATCC MYA-826
-
SwissProt
Manually annotated by BRENDA team
Paracoccidioides brasiliensis Pb01 ATCC-MYA-826
Pb01, ATCC-MYA-826
SwissProt
Manually annotated by BRENDA team
cytochrome c oxidase with catalase activity; gene ctaG
UniProt
Manually annotated by BRENDA team
Paracoccus denitrificans AO1
cytochrome c oxidase with catalase activity; gene ctaG
UniProt
Manually annotated by BRENDA team
microfungus isolated from soil collected in arctic tundra
-
-
Manually annotated by BRENDA team
strain 1, microfungus isolated from soil collected in arctic tundra
-
-
Manually annotated by BRENDA team
Penicillium cyclopium 1
strain 1, microfungus isolated from soil collected in arctic tundra
-
-
Manually annotated by BRENDA team
Penicillium islandicum
microfungus isolated from soil collected in arctic tundra, 2 strains
-
-
Manually annotated by BRENDA team
Penicillium piceum
strain F-648
-
-
Manually annotated by BRENDA team
Penicillium piceum
strains F-648 and A-3
-
-
Manually annotated by BRENDA team
Penicillium piceum F-648
strain F-648
-
-
Manually annotated by BRENDA team
Phaeobacter gallaeciensis BS107
-
A9GRH4
UniProt
Manually annotated by BRENDA team
isoform Cat1
A9QNB6
UniProt
Manually annotated by BRENDA team
isoform Cat2
UniProt
Manually annotated by BRENDA team
isoform Cat3
UniProt
Manually annotated by BRENDA team
Pibocella sp.
strain MH3
-
-
Manually annotated by BRENDA team
Pibocella sp. MH3
strain MH3
-
-
Manually annotated by BRENDA team
enzyme expression is induced under phosphate-limiting growth conditions
-
-
Manually annotated by BRENDA team
strain PAO1, gene katA
-
-
Manually annotated by BRENDA team
strains ATCC 17400 and PAO1, gene ccmC
-
-
Manually annotated by BRENDA team
Pseudomonas aeruginosa PA14 KatA
KatA
-
-
Manually annotated by BRENDA team
strain mt-2. Expression of the katB gene encoding isoform catalase B is specific to the stationary phase and entirely dependent on the rpoS gene, while the katA gene expressed during log phase partially requires rpoS
-
-
Manually annotated by BRENDA team
Pseudomonas putida mt-2.
strain mt-2. Expression of the katB gene encoding isoform catalase B is specific to the stationary phase and entirely dependent on the rpoS gene, while the katA gene expressed during log phase partially requires rpoS
-
-
Manually annotated by BRENDA team
pv. syringae 61
-
-
Manually annotated by BRENDA team
isolated from the drain pool of a plant that uses H2O2 as a bleaching agent
UniProt
Manually annotated by BRENDA team
Psychrobacter piscatorii T-3
isolated from the drain pool of a plant that uses H2O2 as a bleaching agent
UniProt
Manually annotated by BRENDA team
strain VA1, hyperthermophilic archaeon, facultatively anaerobic, gene katPc
SwissProt
Manually annotated by BRENDA team
Pyrobaculum calidifontis VA1
strain VA1, hyperthermophilic archaeon, facultatively anaerobic, gene katPc
SwissProt
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar
-
-
Manually annotated by BRENDA team
enzyme does not show peroxidase activity
-
-
Manually annotated by BRENDA team
Rhodospirillum rubrum S1
strain S1
-
-
Manually annotated by BRENDA team
Roseibacterium elongatum OCh 323
OCh 323
-
-
Manually annotated by BRENDA team
strain EL-88
-
-
Manually annotated by BRENDA team
Roseisalinus antarcticus EL-88
strain EL-88
-
-
Manually annotated by BRENDA team
Sclerotium sp.
isolated from soil collected in arctic tundra
-
-
Manually annotated by BRENDA team
bifunctional catalase-phenol oxidase
-
-
Manually annotated by BRENDA team
type culture Humicola insolens, ATCC 16454
-
-
Manually annotated by BRENDA team
Scytalidium thermophilum ATCC 16454
gene catpo
-
-
Manually annotated by BRENDA team
Serratia marcescens SYBC08
-
UniProt
Manually annotated by BRENDA team
isoform KatA, enzyme expression is induced under phosphate-limiting growth conditions resulting in dramatic increase in H2O2 resistance of wild-type cells, but not of promoter phoB mutant cells
-
-
Manually annotated by BRENDA team
Stenotrophomonas maltophilia WZ2
strain WZ2
-
-
Manually annotated by BRENDA team
Sulfitobacter sp.
strain MH1
-
-
Manually annotated by BRENDA team
Sulfitobacter sp. MH1
strain MH1
-
-
Manually annotated by BRENDA team
strain PCC 6803
-
-
Manually annotated by BRENDA team
gene katG, KatG is a bifunctional enzyme showing both catalase and peroxidase activities
-
-
Manually annotated by BRENDA team
2 enzymes TvC-I and TvC-II
-
-
Manually annotated by BRENDA team
catalase VktA
UniProt
Manually annotated by BRENDA team
strain HY34
-
-
Manually annotated by BRENDA team
Wenxinia marina HY34
strain HY34
-
-
Manually annotated by BRENDA team
pv. phaseoli, gene katA, enzyme KatA is the major enzyme of 2 different occurring in the organism
Q8L2Z7
SwissProt
Manually annotated by BRENDA team
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
24
-
H2O2
-
pH 7.5, 30C
11283
160
-
H2O2
-
catalase covalently immobilized onto Eupergit C, in 50 mM phosphate buffer pH 7.0, at 25C
11283
21000
-
H2O2
-
free catalase, in 50 mM phosphate buffer pH 7.0, at 25C
11283
PDB
SCOP
CATH
ORGANISM
Aliivibrio salmonicida (strain LFI1238)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Enterococcus faecalis (strain ATCC 700802 / V583)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Helicobacter pylori (strain ATCC 700392 / 26695)
Helicobacter pylori (strain ATCC 700392 / 26695)
Helicobacter pylori (strain ATCC 700392 / 26695)
Helicobacter pylori (strain ATCC 700392 / 26695)
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
diffraction to 1.96 A. Comparison with catalase from Proteus mirabilis. In Aliivibrio salmonicida, the major channel leading to the catalytically essential heme group, is locally more flexible and slightly wider, explaining its enhanced catalytic efficiency. The reduced thermal stability of cold-adapted Aliivibrio salmonicida catalase may be explained by a reduced number of ion-pair networks and displacement of the four C-terminal alpha-helices
Q3LSM1
fee enzyme and enzyme complexed with ammonia or NO, hanging drop vapor diffusion method, mixing of 0.004 ml of 12-13 mg/ml protein, containing NH4OH, with an equal volume of the reservoir solution consisting of 45-60 mM magnesium formate, pH 6.7, 2-3 weeks, soaking of crystals in ligand solutions, X-ray diffraction structure determination and analysis at 1.88-1.99 A resolution
P00432
purified catalase form III, sitting drop vapour diffusion method, mixing of 40 mg/ml protein in 0.05 M sodium phosphate, pH 6.8, with reservoir solution containing 12% PEG 4000 and 0.05 M sodium phosphate, pH 6.8, X-ray diffraction structure determination and analysis at 2.69 A resolution
-
crystal structure of the D141E variant determined at 1.8 A resolution
-
purified recombinant enzyme, hanging drop vapour diffusion method, 0.001 ml of 22 mg/ml protein in potassium phosphate, pH 7.0, mixed with equal volume of reservoir solution containing 16-20% PEG 4000, 20% 2-methyl-2,4-pentanediol, and 0.1 M sodium citrate, pH 5.6, 3-5 days, 20C, X-ray diffraction strcuture determination and analysis at 1.8 A resolution, molecular replacement
Q939D2
metal-bound [FeII/FeII]-ADEec and [FeII/MnII]-ADEec enzyme, sitting drop vapor diffusion, room temperature, X-ray diffraction struccture determination and analysis at 2.63-2.8 A resolution
-
homology modeling using crystal structures of human and bovine catalases
A5XB38
mutant M244A, diffraction to 2.0 A, monoclinic space group C2, presence of a dimer in the asymmetric unit
-
ammonium sulfate precipitation
Kloeckera sp.
-
comparison with catalase from Aliivibrio salmonicida LFI1238. In Aliivibrio salmonicida, the major channel leading to the catalytically essential heme group, is locally more flexible and slightly wider, explaining its enhanced catalytic efficiency. Compared with Proteus mirabilis, the four C-terminal alpha-helices of Aliivibrio salmonicida LFI1238 catalase are displaced in the structures, explaining the reduced thermal stability
P42321
hanging drop vapour diffusion method, using 24% (w/v) PEG 2000, 10 mM NaCl, 10 mM CaCl2, and 3% (w/v) 6-aminocaproic acid in 100 mM bis-Tris buffer pH 6.5 at 18C
-
native and recombinant wild-type enzyme and enzyme mutants H82N and V123F, sitting drop vapour diffusion method, mixing of 9.4 mg/ml protein with reservoir solution containing 18% w/v PEG 2000, 0.1 M barium chloride, 0.1 M bis-tris pH 6.8 for the wild-type enzyme, and 6-16% w/v PEG 400, 0.2 M potassium chloride, 0.01 M calcium chloride, 0.05 M sodium cacodylate, pH 5.0-5.6 for the mutant H82N or PEG 4000 instead of PEG 400 for the mutant V123F, X-ray diffraction structure determination and analysis at 2.7 A, 1.4 A, 1.5 A, and 1.9 A resolution, respectively
-
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
chloroform
-
ethanol/chloroform: TvC-I and TvC-II, quite stable to
Ethanol
-
ethanol/chloroform: TvC-I and TvC-II, quite stable to
Ethanol
-
maintains 90% activity against ethanol/chloroform (enzyme solution: ethanol: chloroform = 10: 5: 3)
Ethanol
Rhodospirillum rubrum S1
-
maintains 90% activity against ethanol/chloroform (enzyme solution: ethanol: chloroform = 10: 5: 3)
-
guanidine-HCl
Q8X220
CatP is highly resistant to denaturation by guanidine-HCl. The enzyme conserves activity after 48 h exposure to 1 M guanidine-HCl. Guanidine-HCl at 1.5 M for 48 h decreases the catalase P activity to about 60%
guanidine-HCl
Paracoccidioides brasiliensis ATCC MYA-826
-
CatP is highly resistant to denaturation by guanidine-HCl. The enzyme conserves activity after 48 h exposure to 1 M guanidine-HCl. Guanidine-HCl at 1.5 M for 48 h decreases the catalase P activity to about 60%
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
not sensitive to oxidation by singlet oxygen
-
439785
oxidation by singlet oxygen
-
439772, 439772
sensitive to photooxidation
-
439790